NOL5A (NOP56) (AK097186) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NOL5A; SCA36
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK097186, the custom clone sequence may differ by one or more nucleotides CGAAGGGAACTGAATGAGGACAAGCTGGAGAAGCTGGAGGAGCTGACAATGGATGGGGCCAAGGCTAAGG CTATTCTGGATGCCTCACGGTCCTCCATGGGTCAGTGCAGAGCCTGGCAACCTGCATAAGGTATGGGGCT CTAAATAGCTGGCCTCTTGCATTCACACTTGGTTTTTCCTAGGCATGGACATATCTGCCATTGACTTGAT AAACATCGAGAGCTTCTCCAGTCGTGTGGTGTCTTTATCTGAATACCGCCAGAGCCTACACACTTACCTG CGCTCCAAGATGAGCCAAGTAGCCCCCAGCCTGTCAGCCCTAATTGGGGAAGCGGTGCGTCACAGGGGAC TCAAAAATGGGAGAATAAGGACTGTTGCCATGTGCACCTGCACTGCTGTATTTCGTGACCCACCATGTCT TCCCTAGTTGTGCTTGATGGGGAGGTGGGGAGCAGGGCTGTCGTGCAACTGGGCAGGTCAGCAGTTCATT TCTCTGACTGCTTCCTTGACTCTCTCTCCAGGTAGGTGCACGTCTCATCGCACATGCTGGCAGCCTCACC AACCTGGCCAAGTATCCAGCATCCACAGTGCAGATCCTTGGGGCTGAAAAGGCCCTGTTCAGGTACCAGT GAGGGCACCTGCCCACAATCAGGTGCCACTTCTGGTGCCCACTGCTTGTTGGGGGATCACGGTGATGGCT GACCAGGGCTCCCTGACCTATACAGGCCTCTGCTATGGGGGTGATGGCCAGTCCTGGTGTCTGAGTGATT CCCAGGGCCCAGCAAAGGGACCAAGTTTCCAGGTCAGCGACATTGGATGCCTTCCCTCTGCCTCTGGGAG CTATGGGTTGGCATGCATTGGGGTAGAGATCCAATCTGGCCTGAGGCTCACTCAGGACTTCGGGGTGAGA GGAGGGGAGGAGCTGAGCTGCCTTGGCTAATGGGGTTGAAATTTCTGATCTTAAACTCTCCACTGAATAT TCTCTCAGAGCCCTGAAGACAAGGGGTAACACCCCAAAATATGGACTCATTTTCCACTCCACCTTCATTG GCCGAGCAGCTGCCAAGAACAAAGGCCGCATCTCCCGATACCTGGCAAACAAATGCAGTATTGCCTCACG AATCGATTGCTTCTCTGGTATGGGTGGGGGGGCGTTGGCAGGTGTGAGAAGGGGCTGGGTGGCTGGGTGG GGAGGCTTGCAACCATAGCTTCCACAATGATGGCAATATTTTTCGTCAACAGCAGTTCACCTAGTGAGTG TTGAGACTCTGGGTCTGAGTGAAGCTGAGGGTAGAGGGAACACAGGGTTGGGGTAGTTTCTCTCTTTGGG CTGACAGGCTTTGTCACCCACACACATCCAGAGGTGCCCACGAGTGTATTCGGGGAGAAGCTTCGAGAAC AAGTTGAAGAGCGACTGTCCTTCTATGAGACTGGAGAGATACCACGAAAGAATCTGGATGTCATGAAGGA AGCAATGGTTCAGGTCAGTTGGGCTTTGCTGGGTGTGGAGTGGCATAGCTAGCTGTTGGAGGTGATGAAC TGTCTGAGCCTGACCTTGTAGAATGGAGGCAAAAAAAACTGATTTAATGAGCCTGATCCAATAAAGCCAG AAAGGAGTCCTCAGAGCACCAGAAGTCTTCAGGCCCTTTTAGCACTTTTCTTTGACCAGGCAGAGGAAGC GGCTGCTGAGATTACTAGGAAGCTGGAGAAACAGGAGAAGAAACGCTTAAAGAAGGAAAAGAAACGGCTG GCTGCACTTGCCCTCGCGTCTTCAGAAAACAGCAGTAGTACTCCAGAGGAGTGTGAGGTCAGTAGGCAGC ACGGCCCCGGCAGAGATCCTAGGTTGTAGGATTTTCAACAGCAGAACAAAGGATATGCTGCATCAAGCTG TGGTCTTGAGTCCAGGCTTTTGGACTGAAACAAGGACCTGAAACATCTAAAACTACCTCTAGATTCTATA GGAAGGAGATAGGTGCTGAACTTGCTCAAGAGCCCAGAGAGCTGGTTGTAGCTCACACCCGTTCCCTGGG CATGTGTGTTCTGTCCTCGGCTGCCTCCCAGGAGTCCTCAACCTGGGGTAGTGTAAATTCCTGCTCTGCT TATTATCAGACGTGCGTCCGGAGGTGGTCGTGTTTCACAGTGGGGATGGGGGCAGGGAGGTCCCCAATGT GCTAAGCTACAATCATTCTCCCTGAGATTTTCATTTAGCACCCAGTTTCTTAAACAGTGTTTCAGGGCCC TGTCTGGAACTTGGCATGATGGTTCTGTTGCGACCAGCATGGTGGGTGTTTTTTAGGTTTTTTTTTTTAA TGGGCTGAGGTAATTTCTCATGACATGTTTTCCTTCTAATTTGGGACAGCCTTTGGGGTGGATTTCTAAA GTTATACCCACACAATTAAACTATCCCAGAAACACTGGGCAATGTTAACGACACGCGTTCCCCTGCCTTG GCTACTTAATTGCTGAAGATGTAATGAGCACTGTTCTCACAGCCTGTTCCCCTGTCCTTCCCTTTAGGAG ATGAGTGAAAAACCCAAAAAGAAGAAAAAGCAAAAGCCCCAGGAGGTTCCTCAGGAGAATGGAATGGAAG ACCCATCTATCTCTTTCTCCAAACCCAAGAAAAAGAAATCTTTTTCCAAGGAGGGGTTGATGAGTAGCGA TCTTGAAGAGACCGCTGGCAGCACCAGTATTCCCAAGAGGAAGAAGTCTACACCCAAGGAGGAAACAGTT AATGACCCTGAGGAGGCAGGCCACAGAAGTGGCTCCAAGAAAAAGAGGAAATTCTCCAAAGAGGAGCCGG TCAGCAGTGGGCCTGAAGAGGCGGTTGGCAAGAGCAGCTCCAAGAAGAAGAAAAAGTTCCATAAAGCATC CCAGGAAGATTAGAATGCAAATGGGCATTCTCTGGGAGGTGGGGCATACCATAGCCCAAGGTGACATTTC CCACCCTGTGCCGTGTTCCCCAATAAAAACAAATTCACAAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK097186 unedited CCCCCCCCGGGGNNNCNCCCCNNNNCNCNNCCNCCCCTCCCCCCCNCCNCCCCNNGGGTC CGAGATTTGTNATACGACTTCACTATAGGGCGGCCGCGATTCGGCACGAGGAGGAGATGA GTGAAAAACCCAAAAAGAAGAAAAAGCAAAAGCCCCAGGAGGTTCCTCAGGAGAATGGAA TGGAAGACCCATCTATCTCTTTCTCCAAACCCAAGAAAAAGAAATCTTTTTCCAAGGAGG AGTTGATGAGTAGCGATCTTGAAGAGACCGCTGGCAGCACCAGTATTCCCAAGAGGAAGA AGTCTACACCCAAGGAGGAAACAGTTAATGACCCTGAGGAGGCAGGCCACAGAAGTGGCT CCAAGAAAAAGAGGAAATTCTCCAAAGAGGAGCCGGTCAGCAGTGGGCCTGAAGAGGCGG TTGGCAAGAGCAGCTCCAAGAAGAAGAAAAAGTTCCATAAAGCATCCCAGGAAGATTAGA ATGCAAATGGACATTCTCTGGGAGGTGGGGCATACCATAGCCCAAGGTGACATTTCCCAC CCTGTGCCGTGTTCCCCAATAAAAACAAATTCACAAGAAAAAAAANNAAAANAAAAAAAA AAAAAAAAAAACCTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATC CCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAANGTGCCACTC CAGTGCCCACCAGCCTTGTCCTAAAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTC CTTCTATATATTATGGGGGTGGAGGGGGGGTGGTATGGANCAGGGGGCAAGTTGGGAAAA ACACCTGTAGGGCCTGCGGGTCTATTGGGAACCAAGCTNAGTGCANTGGCACAATCTGGC CTCACTGCATCTCCGCCTCCTGGGTTCAAGCCGATCTCCTGCCTCAGCCTTCCGATTGTT GA
Restriction Sites	NotI-NotI
ACCN	AK097186
Insert Size	4700 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK097186.1
RefSeq Size	2981 bp
RefSeq ORF	2981 bp
Locus ID	10528
Protein Families	Stem cell - Pluripotency
Gene Summary	Nop56p is a yeast nucleolar protein that is part of a complex with the nucleolar proteins Nop58p and fibrillarin. Nop56p is required for assembly of the 60S ribosomal subunit and is involved in pre-rRNA processing. The protein encoded by this gene is similar in sequence to Nop56p and is also found in the nucleolus. Expansion of a GGCCTG repeat from 3-8 copies to 1500-2500 copies in an intron of this gene results in spinocerebellar ataxia 36. Multiple transcript variants encoding several different isoforms have been found for this gene, but the full-length nature of most of them has not been determined. [provided by RefSeq, Jul 2016]

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