MTHFD2L (BC032771) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FLJ13105; methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2-like; MGC45532; MGC72244
Vector	pCMV6-XL6
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC032771, the custom clone sequence may differ by one or more nucleotides ATGGACCCAAGAGTCAGCGGTATATTAGTTCAGTTACCACTACCAGACCACGTTGATGAGCGAACAATAT GCAATGGAATTGCCCCAGAAAAAGATGTAGATGGATTTCATATTATCAATATTGGAAGATTGTGCCTTGA TCAGCATTCTCTCATACCTGCCACTGCCAGTGCTGTTTGGGAAATAATAAAAAGAACAGGAATTCAAACA TTTGGAAAAAATGTGGTTGTGGCTGGAAGATCCAAGAACGTAGGGATGCCTATTGCCATGCTTTTACACA CTGATGGAGAGCATGAACGGCCAGGAGGTGATGCAACTGTGACAATAGCTCACAGATACACCCCCAAAGA GCAACTGAAGATTCATACGCAGCTGGCAGATATTATCATAGTTGCTGCAGAGAGATTTCACCATTTTGCC CAGGACCTCTCCAACTCCTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC032771 unedited CCGCCCCGTTGNCGCATAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC TCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTC GGCACGAGGCCGCCTTGGCCGAGCGCCGGCGTTGGGCAGAAGCACAGCACCCTCCGTAAG GGCACCGGGAGAGCCCGGGAGTGCGTTCCGGGGCTTTCGGAGCAGCGGTGTGAGGACCAG CAGAGAGAAGAGATTCCATCTTCCAGAGACATGAAGCCATTATTATATCAGGAACCGAAA TGGCCAAGCATATCCAGAAAGAAATACAGCGAGGTGTGGAATCATGGGTTTCCCTTGGAA ACAGAAGACCTCACCTCAGTATAATTTTAGTGGGAGATAACCCAGCAAGCCATACATATG TCAGGAATAAGATAAGAGCTGCCTCTGCTGTAGGTATTTGTAGTGAGCTCATTCTAAAAC CTAAGGATGTTTCTCAGGAAGAACTTTTGGACGTAACTGATCAATTGAATATGGACCCAA GAGTCAGCGGTATATTAGTTCAGTTACCACTACCAGACCACGTTGATGAGCGAACAATAT GCAATGGAATTGCCCCAGAAAAAGATGTAGATGGATTTCATATTATCAATATTGGAAGAT TGTGCCTTGATCAGCATTCTCTCATACCTGCCACTGCCAGTGCTGTTTGGGAAATAATAA ANAGAACAGGAATTCAAACATTTGGAAAAAATGTGGTTGTGGCTGGAAGATCCAAGAACG TANGGATGCCTATTGCCATGCTTTTACACACTGATGGAGAGCATGAACGGGCCAGNAGTG ATGCAACTGTGANCATAGCTCACAGATACACCCCCAAAGAGCAAACTGAGATTCATACGC AGCTGGCAGATATATCATAGTTGCTGCAGGTATTCCAAAGTGATACGTCTGATATGGTAA AGAGTGCTGCTGTATTGATGGGGTATCACTATGTCCGATCAGG
Restriction Sites	NotI-NotI
ACCN	BC032771
Insert Size	2300 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC032771.1, AAH32771.1
RefSeq Size	1437 bp
RefSeq ORF	1437 bp
Locus ID	441024
Domains	THF_DHG_CYH

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