Thymidine Phosphorylase (TYMP) (AK057214) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ECGF; ECGF1; hPD-ECGF; MEDPS1; MNGIE; MTDPS1; PDECGF; TP
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK057214, the custom clone sequence may differ by one or more nucleotides ATGGCAGCCTTGATGACCCCGGGAACCGGGGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCC AGGGACTTCCCGACCCTTCGCCAGAGCCCAAGCAGCTCCCGGAGCTGATCCGCATGAAGCGAGACGGAGG CCGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGGGCGCACAG ATCGGTGCGTGGGGAGGGTTGGGCGTTCCTGACCCCGACTGGGAGGTCAGCCCGAGAGACTTTGGGTCCC TGGGGGTGCGACGGTGCCCCACTACCAGCACCGGCCCCAGGGTGCCCCACCGCTGTGGGCTGCCACCCTC ACGCGTACCCCCACATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGAGGAGAC CTCGGTGCTGACCCAGGCCCTGGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCAGCAG CTTGTGGACAAGCATTCCACAGGGGGTGTGGGTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK057214 unedited TATTTTGGTATACGACTTACTATAGGGCGGCCGCGAATTCGCACCAGGCGAACCCTGAAC CCTACGGTCCCGACCCGCGGGCGAGGCCGGGTACCTGGGCTGGGATCCGGAGCAAGCGGG CGAGGGCAGCGCCCTAAGCAGGTACGGGCGGGGCTCAAGTCGCGAGGCGGGGAAGCGGGA GGCAGACACGGACGAGGGCGACACAGACACGGGACCGAGGGGCGGACACCGGAGAGACAC GGGAAAGGGGTCGGGACAGGAGCACGTGGCTCAGACACCGACGCCGGGAGGCCGCAGACC CCGGACGTGTCAGGCATCCCCGCAGGCCCGGAGCGATGGCAGCCTTGATGACCCCGGGAA CCGGGGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGACC CTTCGCCAGAGCCCAAGCAGCTCCCGGAGCTGATCCGCATGAAGCGAGACGGAGGCCGCC TGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGGGCG CACAGATCGGTGCGTGGGGAGGGTTGGGCGTTCCTGACCCCGACTGGGAGGTCAGCCCGA GAGACTTTGGGTCCCTGGGGGTGCGACNGTGCCCCACTACCAGCACCCGGTCCAAGGTGC CCCCACCGCTGTGGGCTGTCACCCTCACGCGTACCCCCACATACCAGGGGCCATGCTGAT GGCCATCCCAACTCCGGGCATTGGATCTGGAAGAGACCTCGGGTGCTGACCCAGGCCCTG GTTTAATCCGGACAACAACCTGAAGTGCCCACAGGCCTTGTCGCCAACAACTTTGTGGAC CAGCATTCCCAAGGGGGTGTGGGTGGAAAAGGCAAACTTGTTCTTTCNACCTGCCCTGAG GGCATTTGGCTTCGAAGGTAAAAAAACACCTTCCTTTCCAAACGGGGACTTATTCGCCAA TGTGAAAACCAATGCATTTCAAAGCGCGTN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK057214 unedited GAGTTTAAATCCTTTTTTTTTTTGTAGGAGAATCAATTTTCACTATATTACCCAGGCTGA TCTCCAACTGGGCTCCTCTGCCTCCCAGAGTGCTGGGACAGGGTCTCACTCTGTTGCCCA AGCTGGAGTGCAGTGGCATCGTCCCGGCTCACTGCAGCCTAAACCTCCCAGGCTCAAGTG ATCCTCCTGCCTCAGCCTCCTGAGTAGCTGGAACCACGTGCGTGCACCACCACACGCAGC GAACTATTTTGTAGAGATGGGGTTTTGCCTTGTTATCCAGGCTGGTTTTGAACTCCTGGG TTCAAGGGATCTGCCCTCTTCGGCCTCCCCAAGTGCTAGGACTACAGGTGTGAGCCACCG TGCCCCGCCAGCCATCTGTGATTTTGACCAACTTCCCATTTTATGATCCTAATCAAAGTT CCACTGATGATTTCGGCCCAAGCCCAGTGACTCATGAGTAACCTTGACCAGTGTTCTCAT TAGTGACCCTAATGATCCACCAGTGATCTTTTAGTGATTCTGGCCAGGGTCTCCATCATC AACCTGGATCAATGACTGATCCGTGGCGCCCCGTACCTGCTCTGGGCTCTGGATGACATT GAATCCAGGAATAGACTCCAGCTTATCCAAAGTGCCTCCTGTGTGCCCCAGACCACGTCC GCTGATCATTGGCACCTGGTGGTCAAGGATGCTGAGTACCCTGCACAGTACCCCTCCCCC ACCTCTGTGGCTCCCTTCACCTTCCCGGGCTAGGAGTGCANCTGGGATTTGGAAGTATTT TAACCANCTTTAGGGGTGCCCAACTGGTGAGGGATGGAAAAGAAGTTTCCCCGATTGAAA TCGTCATTCAGG
Restriction Sites	NotI-NotI
ACCN	AK057214
Insert Size	2250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK057214.1, BAG51886.1
RefSeq Size	2049 bp
RefSeq ORF	2049 bp
Locus ID	1890
Protein Families	Druggable Genome
Protein Pathways	Bladder cancer, Drug metabolism - other enzymes, Metabolic pathways, Pyrimidine metabolism
Gene Summary	This gene encodes an angiogenic factor which promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. It has a highly restricted target cell specificity acting only on endothelial cells. Mutations in this gene have been associated with mitochondrial neurogastrointestinal encephalomyopathy. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Apr 2012]

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