Glutathione S Transferase kappa 1 (GSTK1) (AK095263) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GST; GST13; GST 13-13; GST13-13; GSTK1-1; hGSTK1
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK095263, the custom clone sequence may differ by one or more nucleotides ATGGGGCCCCTGCCGCGCACCGTGGAGCTCTTCTATGACGTGCTGTCCCCCTACTCCTGGCTGGGCTTCG AGATCCTGTGCCGGTATCAGAATATCTGGAACATCAACCTGCAGTTGCGGCCCAGCCTCATAACAGGGAT CATGAAAGACAGTGGAAACAAGCCTCCAGGTCTGCTTCCCCGCAAAGGACTATACATGGCAAATGACTTA AAGCTCCTGAGACACCATCTCCAGATTCCCATCCACTTCCCCAAGGATTTCTTGTCTGTGATGCTTGAAA AAGGTGAAGAGAGTGGGATGTAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK095263 unedited ACATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCAGCATGGGGCCC CTGCCGCGCACCGTGGAGCTCTTCTATGACGTGCTGTCCCCCTACTCCTGGCTGGGCTTC GAGATCCTGTGCCGGTATCAGAATATCTGGAACATCAACCTGCAGTTGCGGCCCAGCCTC ATAACAGGGATCATGAAAGACAGTGGAAACAAGCCTCCAGGTCTGCTTCCCCGCAAAGGA CTATACATGGCAAATGACTTAAAGCTCCTGAGACACCATCTCCAGATTCCCATCCACTTC CCCAAGGATTTCTTGTCTGTGATGCTTGAAAAAGGAAGTTTGTCTGCCATGCGTTTCCTC ACCGCCGTGAACTTGGAGCATCCAGAGATGCTGGAGAAAGCGTCCCGGGAGCTGTGGATG CGCGTCTGGTCAAGGAATGAAGACATCACCGAGCCGCAGAGCATCCTGGCGGCTGCAGAG AAGGCTGGTATGTCTGCAGAACAAGCCCAGGGACTTCTGGAAAAGATCGCAACGCCAAAG GTGAAGAACCAGCTCAAGGAGACCACTGAGGCAGCCTGCAGATACGGAGCCTTTGGGCTG CCCATCACCGTGGCCCATGTGGATGGCCAAACCCACATGTTATTTGGGCTCTGACCGGAT GGAGCTGCTGGCGCACCTGCTGGGAGAGAGTGGATGGGCCCTATACCTCCAGCCGTGAAT GCCAGACTTTAAGATTGCCCGGAGGAACCAACTCTTCGTATAAAAAAGCAGCCATCTGCT TAACCCTGGCTCCCCATAGGCACTGGGACTTCGATTTCTCTTCTGATAGAGTATTTCTGT GGCCCTGGAGCTTCTGTCTTTCCCTACCCCAGATGCCAGAGACGTCCCCTAACCAGTGGA CCTTACTTATGCTCACAAGCTTTAAAACCCCATTTGTTTCCACAAAAACC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK095263 unedited ATTACTATGNACCGCGTGCCGCAAGCTCANGATCGGTTTTTTTTTTTTTTTTTTGTTTTG CCTGATGGCATTAGGTTTATTTTGTGGGAAAGCAGAATTGGGGCTCTCTGAAAGCACTTG TGAGGCATAGAAGTAAAGGTTGCCACATGGCTAATGGTGGACGTCTTCCTGGCATCCTTG GGGGTAGGGGAAAGACAGACAGCTCCCAGGGCCACAGAAAATACCTCTATCAGATAGAGA AATCCGAGTCCCAGTGCCTTATGGTGGAGCCAAGGGTTAAGCAGATGGCCTGCTTTTTTT ATACGAAGAGTTTGCTTCCTCCGGGCAATCTTAAAGTCTGGCATTCACGGCTGGAGGTAT AGGGCCCATCCACTTCTCTCCCAGCAGGTGCGCCAGCAGCTCCATCCGGTCAGAGCCAAA TAACATGTGGGTTTGGCCATCCACATGGGCCACGGTGATGGGCAGCCCAAAGGCTCCGTA TCTGCAGGCTGCCTCAGTGGTCTCCTTGAGCTGGTTCTTCACCTTTGGCGTTGCGATCTT TTCCAGAAGTCCCTGGGCTTGTTCTGCAGACATACCAGCCTTCTCTGCAGCCGCCAGGAT GCTCTGCGGCTCGGTGATGTCTTCATTCCTTGACCAGACGCGCATCCACAGCTCCCGGGA CGCTTTCTCCAGCATCTCTGGATGCTCCAAGTTCACCGCGGTGAGGAAACGCATGGCAGA CAAACTTCCTTTTTTCAGCATCACAGACCAGAAATCCTTGGGGAAATGGATGGGAATCTG GGAGAAGGTGTCTCACGAGCTTTAAGTCATTGCCCTGGTTAGTCCTTTTGCGGGAAGCAG ACCTGGAGCTTGTTTCCACTGGCTTTCTGATCCCCGTTATGAGGCTGGCCGCAACTGCCG GTGATGGTTCCAAATTCTGGTACCCGCCCAGAT
Restriction Sites	NotI-NotI
ACCN	AK095263
Insert Size	1000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK095263.1, BAG53018.1
RefSeq Size	2692 bp
RefSeq ORF	303 bp
Locus ID	373156
Domains	DSBA
Protein Pathways	Drug metabolism - cytochrome P450, Glutathione metabolism, Metabolism of xenobiotics by cytochrome P450
Gene Summary	This gene encodes a member of the kappa class of the glutathione transferase superfamily of enzymes that function in cellular detoxification. The encoded protein is localized to the peroxisome and catalyzes the conjugation of glutathione to a wide range of hydrophobic substates facilitating the removal of these compounds from cells. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009]

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