RABL4 (IFT27) (AK090708) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BBS19; RABL4; RAYL
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK090708, the custom clone sequence may differ by one or more nucleotides AACACTCAGTAGATGTTAGCTTTCTTAAAGTATAAATATTGCCCCTAGAATCTTGTTTATACTGTTATTA CTTACTAAATCCTTGTATTCATGGCTTGTGCAGAGATAAATATCACCCTTGCCTCTGGTCCAATTATCAC AAATGCTAAATTAATGAAGCCCAAATCAGCTTTTCCTGTATAGAGGGTTTGCCATCTTTTCTGCAGCACA CCTTTGTGCAAAGCAGTAGGCGGTAGTGCCCTAGCAAACAGGATTTCAGTATTCTCCCTTCCTCCATTCA TTTGTGAAATTGTCCCTGCACCCCAATATTCCATATTCCCTAGAACGCTTGATAGAACTCAGAAATTAAA ACCCACCCCAACCTCCAAAACATGAGTCACCAAAGGCCAGTGGGCTACGGGTTAAACTCTGTTGAGGTGT TGAAGTGGGTGGGTGGGTGTGAACTACGCTTGAAGAGGCTGCAGTAAGCACTTGCTATTATCTGTGCAAG TTAACTAGTCCCCCCTGAGTTGAGGCTCACAGAAGCACCGCTGGCAGCTCGGTTAAGAAAGCTGTAATTA AAAAGGAAATATAAGCAAACGCAGCTTGAGACGCAGCCAAGGGCTGGCGCCAAGCTCATCTCCTCCTCTC TTCTGCCTAAACACCGCAGTGACCATCCTGCCTACCCAAAGGAGGTCCCTACAAACAAGTGCAAGAACAA TCCGCGCTGATCACTGCCCTCCCAGGCAAGCGAGGGGTGGGGAAGTAACGTTTGATGAGGGCCTACTGTG TGCTAGGTGTTTTGCACACATTATTTGTTTAGGCTTCATAACAGCCCTACAAGGTAACACTCCTGTGTTG TATAGATGACAGACTCAGAAAGGTTGTGTAGCTTGCCCTGGTTGCATAGTTAAACGAGGGCTAGAAACAG GACTAGGAGTCAGGCCTGTCCAGCTGGAAAACTTGGGTTTTCTAGAAGGGGTACCCTGGCCTCCTGCGGA GCCTGCTGTGGGACTCTGCAGAACACAATTCAAGGCCAGACTGAACACTAGCCTGAACCTGCCCTGAGAA TCCCTCTAAGCCGACCTACTCCACAGCTGTCCTGACTGTGTAAGCGAGATGATGATTAGTGATCAGACGA AAGGATTCCTGTCATTGGTAACCCTCTCAAAGTATTTGGAAAACAGTTCAATTTTCATCTATTTCAGAAG CACGCCGTGGTGTCTATTGAGGCTCACCTGCATTGAATTCCTTCCTTTTTATGTTGCGATCTCCCAAGAT TGCATTGTGGAGTGTTTTCGAATCCATTTTGAAATCCCCGTGCGTGCGCTATGCAGGCCTCAGTCTTTTT CCATTCCATTCTTAACTCTACTTTCGACGGAAGCAGTGTTTTACCCCGACACTGGCTTGCCTAGGACCTT GTGCTCTGCACAACTAGCAGGGCCCGGCAGGATGTACTGAATTCTTGCTCTCGTGTCCAGCTGGACGGTG ATGGCTTTCAAGTCCTTGGCTGTTGGGAGCTTACTATAAATGTTCGTCTTGGCTACAAACTCTCCACTCT CTCCTCGGCACTCTCTCAGCATTGCCACCACTGTCTTTCCTCTTGGCCAACTGTTTTCTTTACTTAGGCT TTCCCTTGCTAGAAAGTCCAGGTAACTTTCTCCACGGGACCTGGTTTCCTTCGCACATCCCAGCTGGCCT CGAGGAAAGGTAGCTCTTCCCAAATCAGAGAATCTGGATGCTGGGCTGGGCTCTGCACCAACCAGCTGGG CCGCTTCACCCGCTGGGCCCCAAACTACTCATCTGTGAAGCGAAGGCACCGCGCTTGATGCCTTCTGCAA CGTTCTTCAGTTTGGAAATCCTTCTGTTTCGTTGGGGATATTTCACGGCCTCTTCTCAAGGTTGCACTTT TGCCAGCTGCCAGGGATCGTCTCAAAACAGGTTCTTAGTGCATTCATAGCTTGAGCTGCTGTCTTGAAAG TAGTACATTCCTTTTTCTGCCAACTTTTTTCTGAGAAAGTTTTTGAATGCACACGTGCACCCAACAGAGT GAGAGTGGCTGTTAAGAGAGAGGGCGCCATTTCCTTTGCCCTCCAGCCTGTCCCTGTGCACCCTGGAGGG GCCCGTTTTTTCCACCGCTTAGATAAAATCTAGGGCAAGTTCCTGAACTTCTCTTGTCTCTCTCAGGTAA CAAAAATTCTTTTGGGCTCCTTTAGTCACAAAGATATTCACGATTTCAGGTATTAAAGTGCCCAGCCCTG GGTGATTGTCAAAATTCTGAACTTGATTTAAAGTGGCACCTCCTCTCACAGTCTTCGGGAGGGAGAGACC GGAGCCAGGAGTGCAGCGTGTTTGCTGGGGTCTGTCGTGGCCCACTCCACACCTGCTGGGTGGATCCGGC TGGTGCCCCATGGGCGCCTCTGAGATGCCCCTCCCCACCCCATCAGTGGCGCTGTCTCACCTGCAGGCTG CTTCTCACAGGTGGTCCCCCCTCACTCCTCCTGCAGCCCCAGTTCCTGGCTGTTCATTCTTATTGGGACC CGTCACCCTCCTGGAGGCGGTCCCAGCCGAGCCCCCTTAAGACAGCACCAGGCTGGCTCCACTTGGCCCC CGCTGGTTCAGGGAAGTGCTGCTGCAGCCGTTTAGTTTGACAAAGGAGGCAGCGAGGCCGTCTCATTGGT AGCCCTCTCCTGGCTTGCCCAGCCACCACCTCACCTCGATTCCTCCCAGGCCTGGGTCCAGCACCAGCCT AGGAAGAGGGTGCCCCATGCTGTCTAGCTCTTCTTCGGGATGGGGGGCTCCAGGTTCCTTGGTATTTTGC TTTGGCCTTTGGAGCCTCAGTCAAAACTGAGGAAAGGTGTCATTTTCACATCTCGTCACACGTACAGTGA CTGCAACTAAAAGCACAGGCTTTGTAGAAACAGACATGGGTTCAGGCCCCAGCTCCACCATTCACAAGGT GTGTGGCTTCCTGCAAGGTACCTTCATCTCTGAGTTACCTGACTCCATCTGAGTTTCCTTCTTGTAAAAC TGGCATCCGTGAAAGTGGCTACCTCGAAGGGCGTGAAGATGAAATGAGGTGGAAAGTAGGTAGCCCCCGA ATGAGGGAAGCATTGAGTGAGAGCTGGCCCTCTGACCCTTCTAAAAGAACACAGCCAACTTTTTAAACTG TCTTTCCAGAAAGAGATGGAAAACTTCGAAGCCCCTTTCCACTGCCTTGCCAAGCAGTTCCACCAGCTGT ACCGGGAGAAGGTGGAGGTTTTCCGGGCCCTGGCATGACGAGCTGGAGCAGATCGTGCTGCACAACCGGA GAAGACAGAATTACCTCTGCTCTTTTAATATATAATGATGGCTTTAAATAAAATTAGGAGAAAATGTC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK090708 unedited CACATTGTATACGACTATTAGGCGGCCGGATTCGCCGAGCTCCCTTCTCCTTCTTTGTGA AATGTCCCTGCACCCCAATATTCCATATTCCCTAGAACGCTTGATAGAACTCAGAAATTA AAACCCACCCCAACCTCCAAAACAGGAGTCACCAAAGGCCAGTGGGCTACGGGTTAAACT CTGTTGAGGTGTTGAAGTGGGTGGGTGGGTGTGAACTACTCTTGAAGAGGCTGCAGTAAG CACTTGCTATTATCTGTGCAAGTTAACTAGTCCCCCCTGAGTTGAGGCTCACAGAAGCAC CGCTGGCAGCTCGGTTAAGAAAGCTGTAATTAAAAAGGAAATATAAGCAAACGCAGCTTG AGACGCAGCCAAGGGCTGGCGCCAAGCTCATCTCCTCCTCTCTTCTGCCTAAACACCGCA GTGACCATCCTGCCTACCCAAAGGAGGTCCCTACAAACAAGTGCAAGAACAATCCGCGCT GATCACTGCCCTCCCAGGCAAGCGAGGAGTGGGGAAGTAACGTTTGATGAGGGCCTACTG TGTGCTAGGTGTTTTGCACACATTATTTGTTTAGGCTTCATAACAGCCCTACAAGGTAAC ACTCCTGTGTTGTATAGATGACAGACTCAGAAAGGTTGTGTAGCTTGCCCTGGTTGCATA GTTAAACGAGGGCTAGAAACAGGACTAGGAGTCAGGCCTGTTCAGCTGGAAAACTTGGGT TTTCTAGAAAGGGTACCCTGGCCTTCTGCCGAGCCTGCTGTGGGACTCTGCAGAACACAA TTCAAGGCAGACTGNACACTATCCTGAACCTGCCCTGAAAATNCTCTTAGCCGACCTACT TCACAGCTGTTCTGACTGTGTTAGCGAGATGATGATTAGTGATCAGACGAAAGGATTCTG TCATTGTTACCCTTTCAAATTTTGGAAAACAGTCATNTTTATCTTTTCAAACCCCCCGTG TGCTATTGAGCTACCGC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK090708 unedited NGGGAATTACTATGNACCGCGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTTTT TTGACATTTTCTCCTAATTTTATTTAAAGCCATCATTATATATTAAAAGAGCAGAGGTAA TTCTGTCTTCTCCGGTTGTGCAGCACGATCTGCTCCAGCTCGTCATGCCAGGGCCCGGAA AACCTCCACCTTCTCCCGGTACAGCTGGTGGAACTGCTTGGCAAGGCAGTGGAAAGGGGC TTCGAAGTTTTCCATCTCTTTCTGGAAAGACAGTTTAAAAAGTTGGCTGTGTTCTTTTAG AAGGGTCAGAGGGCCAGCTCTCACTCAATGCTTCCCTCATTCGGGGGCTACCTACTTTCC ACCTCATTTCATCTTCACGCCCTTCGAGGTAGCCACTTTCATGGATGCCAGTTTTACAAG AAGGAAACTCAGATGGAGTCAGGTAACTCAGAGATGAAGGTACCTTGCAGGAAGCCACAC ACCTTGTGAATGGTGGAGCTGGGGCCTGAACCCATGTCTGTTTCTACAAAGCCTGTGCTT TTAGTTGCAGTCACTGTACGTGTGACGAGATGTGAAAATGACACCTTTCCTCAGTTTTGA CTGAGGCTCCAAAGGCCAAAGCAAAATACCAAGGAACCTGGAGCCCCCCATCCCGAAGAA GAGCTAGACAGCATGGGGCACCCTCTTCCTAGGCTGGTGCTGGACCCAGGCCTGGGAGGA ATCCAGGTGAGGTGGTGGCTGGGCAAGCCAGGAGAGGGCTACCAATGAGACGGCCTCGCT GCCTCCTTTGTCAAACTAAACGGCTGCAGCAGCACTTCCCTGAACCAGCGGGGGCCAAGT GGAGCCAGCCTGGTGCTGTCTTAAGGGGGCTCGGCTGGGACCGNCTCCAGNAGGGTGACG GGTCCC
Restriction Sites	NotI-NotI
ACCN	AK090708
Insert Size	3500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK090708.1
RefSeq Size	3358 bp
RefSeq ORF	3358 bp
Locus ID	11020
Protein Families	Druggable Genome
Gene Summary	This gene encodes a GTP-binding protein that is a core component of the intraflagellar transport complex B. Characterization of the similar Chlamydomonas protein indicates a function in cell cycle control. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]

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