ZNF75 (ZNF75D) (AK091687) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	D8C6; ZKSCAN24; ZNF75; ZNF82; ZSCAN28
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC103538 sequence for AK091687 edited (data generated by NextGen Sequencing) ATGGCGATGAGAGAGCTGAACGCGGATTCATGCTCAAGCCCCCAGATGGGGGCTATGTGG GAGACTAGTGGGTCTGTGAAAGAGAACTCCAGTCAGAGTAAGAAATACAGCACAAAAATA GAGAATCTTGGTCCTGAGAGCGCTTGCAGGCACTTCTGGAGCTTCCGTTATCATGAAGCA ACCGGACCGCTTGAGACTATCAGCCAACTTCAGAAATTGTGCCATCAGTGGCTGAGGCCA GAGATCCACTCAAAAGAGCAGATCTTGGAAATGCTGGTGTTAGAGCAGTTCCTGAGCATT CTGCCCAAGGAGACCCAGAACTGGGTGCAGAAGCATCATCCACAGAATGTCAAACAGGCT CTGGTCCTGGTGGAATTCTTGCAGAGGGAGCCTGATGGAACAAAGAATGAGAGTTTGTTG ACATTTGAAGATGTGGCTGTGTATTTTTCTGAGGAAGAGTGGCAATTATTGAATCCTCTT GAGAAGACTCTCTACAATGATGTAATGCAGGATATCTATGAGACTGTCATCTCTCTAGGG TTAAAGCTAAAAAATGACACTGGAAATGATCATCCTATATCTGTTTCTACATCAGAAATA CAAACATCAGGATGCGAAGTATCAAAAAAGACCAGAATGAAAATTGCCCAGAAAACAATG GGCAGGGAAAATCCTGGTGATACACACAGTGTACAGAAATGGCATCGAGCTTTTCCAAGG AAGAAAAGAAAGAAACCTGCAACTTGTAAACAAGAGCTTCCAAAACTTATGGATCTTCAT GGGAAAGGCCCCACAGGGGAGAAACCTTTTAAGTGTCAGGAATGTGGGAAAAGCTTCAGA GTTAGCTCTGATCTTATTAAACACCACAGAATTCACACTGGAGAGAAACCCTATAAATGT CAACAATGTGACAGGAGGTTTAGATGGAGTTCAGATCTTAATAAGCACTTCATGACCCAT CAAGGAATAAAACCATATAGATGCTCATGGTGTGGGAAAAGCTTTAGTCATAACACAAAT CTACACACACACCAAAGAATTCACACAGGAGAGAAGCCCTTTAAATGTGATGAATGTGGA AAAAGATTCATTCAGAACTCCCACCTTATTAAACACCAGAGAACTCACACAGGTGAGCAG CCTTATACGTGTAGCTTATGCAAGAGAAACTTTAGTAGGCGATCGAGCCTTCTTAGACAC CAGAAACTCCACAGAAGAAGGGAAGCATGTCTAGTGTCTCCAAACTGA Clone variation with respect to AK091687.1 941 g=>a 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK091687 unedited GGGTGCCCATATAGTAAACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGGTCAGGT CTTCGGGAGGTGGAGGGATCGAATGAGGGAACTGATATTCCAAGACTCCCCACATCTTGG AGCTTCACTCGCCAGGCATCCCGCGCTTTCCTGACGTCGCTTCCCCTAGTGGTGCAGCAG CGGAGAGCTTTGCTACCCAGAGCAGCAGGGAGGCGTGGCCACAGAGGCAGAGGCGTAGTC GAGCCGAGGAAGAGGAAAGTGGCCTCCGGGGAAGCCAGAGCTGACTGAAGTGCTCTTGGG AAGAACAATTCATTGATTTGGTACCAACCTGATGCACCCTGATTTGTCAAAGGTGGTACC AGGTGGGTGTCAGTGTCACATACAAGAGTAAAACTGTTCCTAGAGCAAATGGCGATGAGA GAGCTGAACGCGGATTCATGCTCAAGCCCCCAGATGGGGGCTATGTGGGAGACTAGTGGG TCTGTGAAAGAGAACTCCAGTCAGAGTAAGAAATACAGCACAAAAATAGAGAATCTTGGT CCTGAGAGCGCTTGCAGGCACTTCTGGAGCTTCCGTTATCATGAAGCAACCGGACCGCTT GAGACTATCAGCCAACTTCAGAAATTGTGCCATCAGTGGCTGAGGCCAGAGATCCACTCA AAAGAGCAGATCTTGGAAATGCTGGGTGTAGAGCAGTTCCTGAGCATTCTGCCCAAGAGA CCAGNNACTGGTGCAGAAGCATCATCCACAGAATGTCAAACAGGCTCTGGTCCCTGTGGA ATNCCTGCAGAGGAGCCTGATGGAACAAAGATGAGTCACCAGCCATGAGCTGGAAANGNA GCAGTGCTCTTGGGAGGACAGCAGTGGCCCAAGNCTTCAAGTGAAGCCAGCANACCCCAA CCATGGNTGTGTCC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK091687 unedited CTATGGAACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTGATTTGGT AACAAAGTTTTGTTTATTGTCTAAATTTAAGATAAAATGTGTCATTTTCATTACGAAGTA CAATTTTATGGAATACTAAATAAACATATATAAATAAGCCTTGGCCAACATTATAAACCA GGATCTGACATAGATATAAATCCATGCCTTCCAACTTCCCTTGACGTCACATTATCTTCT CTTAAGAGATAGTCACTTTGGCTAATAGGGAAAAGAAAACCAGTAACATCAGAAAACAAT TTCCATCTGGCCTGGCTCTGACTGGCATCATCACTGTGAGCCATATGCCTTACTTTTCTC TGACACTTCAGGATGTTCCCTCCTGGTATCAGCTGTGCACCAAAGCTAAAAGTTCACAAG GCTCTCCTTGCCATTCTCAATAAATCAGTTTTGCAAAATCTCCAGGCCTTCTTGCAGAGT ATTCAGGCATATAGGGTTGTAGTTGATCAGCCATAAAGATGGGGCTCCCTTTAGGTCCTG CAGCAGTATGGCACCAGTTTGAAAGCCTCCATCCAGTCCACATAATGAAAGTCAGAAAAA GACCATTTACCACTTATCTGTGCTTTCTACAAAAAAAAAACCAGATACCTGGATGAGGGC CAAGACTGCCTCCCTCATCATTTCACTATATGTCTATGTTGACCCAAGTCAGCAGAGGAG AGATATCAAGTGCTTGCAAGGTCCCTAGGCCTGTCCTTACATTCAGACCTTCCCTTGCTA CTTGTAATTCCCTGACAGAAGACATTGGGTAGTGGGTACTCAGGAGAGAAGCACAGAATG GGACAATTCTCCAGGGCAACACTGAATCTCTCTTTCTGTACAGAGTAAGTCCCTGAACCT GGCCTGATTGTCAGTANACGATGATACTATGAACGTCCTAATTAGAATCTCCGG
Restriction Sites	NotI-NotI
ACCN	AK091687
Insert Size	3500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK091687.1, BAG52399.1
RefSeq Size	2976 bp
RefSeq ORF	2976 bp
Locus ID	7626
Domains	KRAB, LER, zf-C2H2
Protein Families	Transcription Factors
Gene Summary	This gene encodes a protein that likely functions as a transcription factor. The protein, which belongs to the ZNF75 family, includes an N-terminal SCAN domain, a KRAB box, and five C2H2-type zinc finger motifs. Another functional gene belonging to this family is located on chromosome 16, while pseudogenes have been identified on chromosomes 11 and 12. Alternative splicing results in multiple transcripts variants. [provided by RefSeq, Jun 2010]

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