NUMBL (AK096566) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK096566, the custom clone sequence may differ by one or more nucleotides CACACTCTTGCACAGTCTGACAACACACACAGACCCACATTTCTACAGACGACAGCCACACTTTCACAGA CAGCACCCGCGGGGCCAGGCTCTCACAGGGAGCTACGGTTGTGTACAAACATGCACACAGTCTCACAAAG AACACCCAGCACTCTCTCAGGCAACAGTCACACCCTTACAAATAACCCTCATGAAAACACTCTCAAAGAC AACCTCATGTTCCCACACACCCTAACGGATGCACCACTGCACACAGATACACCCTCTGTGCAATACCCAC ACACCCACACTCTAAGGCAACAGCCACAGTCACACAGGCTCACCGCCAACAGCTGCACTCTTGGACACAG ACAGTCTTGTAGATCCCTCAGATGCACTCTCCGAGGCAATGTCATCACAGACAATAGCCACACCTTCACA CAGAGACATAGCTACATGGATAACAGCCATGGACACACCCTTTGAAAGACGACAGCCTCATCTCAAACAC AGACACACCCTCACAGGCAACAGGCACTGAGCCACAAGTTCTCATAGACAACAACGGCACTCTCGGATAT AGACAGCAGACACACACTCACAACCACTCTCAGACATCGATGCCCTTCAGCAACAACCTTCAGGGCCTCA GACTCACAGACAACAACCACACTTTTGGATGCATCCCTCACGGACAGCATTCACAGATGCACACTATCCT ACACCATTCTCAGACATGCGTTCTTCCAGACAGCCTTCAGAAACACACTCACAGACATTACTTGCCTGCT CAGATATAGAGCCCCTCATAGACAGCGTTCCAGAACATACCATTGCACACTTACTTACACACACACACAC ACACACACATCCCCCTCTGCTGTTGGTGCTCCCAGTCCTGGGACTGTCAGGATGGCTGATCAGTGAAGAC TGTCCTAGTGGCTGGCCACCTCCACACCCACTCCACACACCCACAGCCCTGCCTCTGACAGTCATCAGTG CAAGGCCCCAGAAGCCTTGCCTGGGGACTAGACACAGTTGTCACAGAATGGCTGTGTCTCAGAGTTCCAG AGACAAGCCACCATCCCCCATACCCCATGCACCTGACTCCTGCACCCCTGACCCATGCAATTCCCAACTC CTCTGCTGCCCTCTGATCACCCCACTGCCTGACTCTCATACCCCTAAAACCCCTGACCGTGCGGCCCCTG ACTTCTATGCCCTCTAGCCCCCATGCCCTCCTACGTCTGATTTCCCACCCTTTGTGTCCTCACGGCCCTG GGAATGAGGAAGCATCCACCACATGGACAAGAAGCCCTCCAATGCCCTGAAGCCTGTGCCCCATACCCCC CACCCCAGACCTCCAGCCCCTTAGCCCCATGGTTCTGAATCCCCAGTGCCCAACTTTCGCACTCCCCTGG CTTCTGTTGGCTCCTTTCCTCACTGTCCCCTGACTTGAGCTGCTGATCCCATGTGCGCTGCCAACTGACC CTGTAACCCCGCTTCCAGTTATCTTGTTATCTGACCCCATGCTCCCTGAGCACTGGTCCCTCCCCGTTGA GCTCCATACCTTCTGACCCCCACCCTTGACCCACATCACTGTCCTGTGTCACCAGACGGGGCGGGCACCA TGAACAAGTTACGGCAGAGCCTGCGGCGGAGGAAGCCAGCCTACGTGCCCGAGGCGTCGCGCCCGCACCA GTGGCAGGCAGACGAGGACGCGGTGCGGAAGGGCACGTGCAGCTTCCCGGTCAGGGTGAGTGGGCGCGGC AGGGTGCGGCGGGGCGAGGGGGACGGGGGCCGCTGTGCTGTGCTGTGCGGGTGCGCGGCCTGGCTGGGAC ACCAGCCCAAGTCGTTTGAAGTTGGTGTTAAAAAGCGTGAGGCCGGCAGTCCTGGCTATCACGGTGAAAC CCCGGCTCTACTAGAAATACAACAAATTAGCCTCGCGTGGTGGCGGGCGCCTGTAGTCCCAGCTACTGGG GAGGCTGAGACAGGAGAATCGCTTGAACCCGGGAGGCGGAGATTGCAGTGAGCCGAGATAGCGCCATTGC ACTCCAGCCTGGGCGACAGAGCAGACTCTGTTTC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK096566 unedited TCACAATTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCGGTTGTGTACA AACATGCACACAGTCTCACAAAGAACACCCAGCACTCTCTCAGGCAACAGTCACACCCTT ACAAATAACCCTCATGAAAACACTCTCAAAGACAACCTCATGTTCCCACACACCCTAACG GATGCACCACTGCACACAGATACACCCTCTGTGCAATACCCACACACCCACACTCTAAGG CAACAGCCACAGTCACACAGGCTCACCGCCAACAGCTGCACTCTTGGACACAGACAGTCT TGTAGATCCCTCAGATGCACTCTCCGAGGCAATGTCATCACAGACAATAGCCACACCTTC ACACAGAGACATAGCTACATGGATAACAGCCATGGACACACCCTTTGAAAGACGACAGCC TCATCTCAAACACAGACACACCCTCACAGGCAACAGGCACTGAGCCACAAGTTCTCATAG ACAACAACGGCACTCTCGGATATAGACAGCAGACACACACTCACAACCACTCTCAGACAT CGATGCCCTTCAGCAACAACCTTCAGGGCCTCAGACTCACAGACAACAACCACACTTTTG GATGCATCCCTCACGGACAGCATTCACAGATGCACACTATCCTACACCATTCTCAGACAT GCGTTCTTCCAGACAGCCTTCAGAAACACACTCACAGACATTACTTGCCTGCTCAGATAT AGAGCCCCTCATAGACAGCGTTCCAGAACATACCATTGCACACTTGCTTAACACACACAC ACACACACATNNCCCTTTGCTGTTGGTGCTCCCAGTCCTGGGACTGTCANGATGGCTGAT CAGTGAAGACTGTNCTAGTGGNTGGNCACTNCACACCACTNCAACACCCACAGNCCTGCC TNTGAAGTCATAGTGCAAGCCCCAAAGCCCTGCTGGNGACANAACAGTGTCCAGAATGCT GGTCTCA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK096566 unedited GTACCGCGGCCGCATCTANATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTGAAACAGAGT CTGCTCTGTCGCCCAGGCTGGAGTGCAATGGCGCTATCTCGGCTCACTGCAATCTCCGCC TCCCGGGTTCAAGCGATTCTCCTGTCTCAGCCTCCCCAGTAGCTGGGACTACAGGCGCCC GCCACCACGCGAGGCTAATTTGTTGTATTTCTAGTAGAGCCGGGGTTTCACCGTGATAGC CAGGACTGCCGGCCTCACGCTTTTTAACACCAACTTCAAACGACTTGGGCTGGTGTCCCA GCCAGGCCGCGCACCCGCACAGCACAGCACAGCGGCCCCCGTCCCCCTCGCCCCGCCGCA CCCTGCCGCGCCCACTCACCCTGACCGGGAAGCTGCACGTGCCCTTCCGCACCGCGTCCT CGTCTGCCTGCCACTGGTGCGGGCGCGACGCCTCGGGCACGTAGGCTGGCTTCCTCCGCC GCAGGCTCTGCCGTAACTTGTTCATGGTGCCCGCCCCGTCTGGTGACACAGGACAGTGAT GTGGGTCAAGGGTGGGGGTCAGAAGGTATGGAGCTCAACGGGGAGGGACCAGTGCTCANG GAGCATGGGGTCAGATAACAAGATAACTGGAAGCGGNGTTACAGGGTCAGTTGGCAGCGC ACATGGGATCAGCAGCTCAAGTCAGGGGACAGTGAGGAAAAGAGCCCACAGAAGCCAGGG GAGTGCGAAAGTTGGCACTGGGATTCAGACCATGGGGCTAAGGGGCTGGAGTCTGGGGTG GGGGTATGGGCACAGGCTTCAGGCATTGGAGGGCTCTTGCCATGTGGGGGATGCTCCTCA TCCCAGGGCCCTGAAGACACAAGGTGGGAAATAAACTTAGAAGGCATGGGGCTAAGGGAT AAAATAGGGGCCCACGGCCGGGGTTTATGGGTTAAGATCAGCCCGGGGGATCAGAGCACC CAAGATTGGGATGCTGGTTCGGT
Restriction Sites	NotI-NotI
ACCN	AK096566
Insert Size	2000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK096566.1
RefSeq Size	2064 bp
RefSeq ORF	2064 bp
Locus ID	9253
Protein Pathways	Notch signaling pathway
Gene Summary	Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of embryonic neurogenesis. Also required postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. Negative regulator of NF-kappa-B signaling pathway. The inhibition of NF-kappa-B activation is mediated at least in part, by preventing MAP3K7IP2 to interact with polyubiquitin chains of TRAF6 and RIPK1 and by stimulating the 'Lys-48'-linked polyubiquitination and degradation of TRAF6 in cortical neurons.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.