GLUR3 (GRIA3) (BC032004) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	dJ1171F9.1; GLUR-C; GLUR-K3; GLUR3; GLURC; glutamate receptor, ionotrophic, AMPA 3; glutamate receptor C; glutamate receptor subunit 3; MRX94; OTTHUMP00000024261; OTTHUMP00000024262
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC032004, the custom clone sequence may differ by one or more nucleotides ATGGCCAGGCAGAAGAAAATGGGGCAAAGCGTGCTCCGGGCGGTCTTCTTTTTAGTCCTGGGGCTTTTGG GTCATTCTCACGGAGGATTCCCCAACACCATCAGCATAGGTGGACTTTTCATGAGAAACACAGTGCAGGA GCACAGCGCTTTCCGCTTTGCCGTGCAGTTATACAACACCAACCAGAACACCACCGAGAAGCCCTTCCAT TTGAATTACCACGTAGATCACTTGGATTCCTCCAATAGTTTTTCCGTGACAAATGCTTGTCCTGCTGAAA GGGACTACCTGCCTTGGCCAGGAAGCATCAGGGAAAACAATTGGACAGCTCTGCCGTGCTGCAAAGATCA TGGGCTGCTGCACCTAAAATGTTCACCAGGTGGGGCCCGCCAAAACTGGGCCTATTGTATCTGGGGCGTT ACAGGTGAACTGTAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC032004 unedited GTGTCAAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGGAGAGA GAGGGAGCAAGAAAGGAAGAGAGAGCGAGCGAGAGAGAGCGAGCGAATAAGAGAGAGAGT AAGAGGGAGAGAGAAGAAGAGGAAGAAGAGGAGGCGGCGGCAGCGGAGGAGGAGGAGGAC TAGTGTGGGGTGGAAAGGAAGAGTGAGCGAGAGCAAGTTAAGGGGAGGGGGTGTAAGAGC CAGCGAATTCTTTTTCTTTTTCTATTATTATTTTGACGACTCCTGAGTTGCGCCCATGCT CTTGTCAGCTTCGTTTTAGGCGTAGCATGGCCAGGCAGAAGAAAATGGGGCAAAGCGTGC TCCGGGCGGTCTTCTTTTTAGTCCTGGGGCTTTTGGGTCATTCTCACGGAGGATTCCCCA ACACCATCAGCATAGGTGGACTTTTCATGAGAAACACAGTGCAGGAGCACAGCGCTTTCC GCTTTGCCGTGCAGTTATACAACACCAACCAGAACACCACCGAGAAGCCCTTCCATTTGA ATTACCACGTAGATCACTTGGATTCCTCCAATAGTTTTTCCGTGACAAATGCTTTCTGCT CCCAGTTCTCGAGAGGGGTGTATGCCATCTTTGGATTCTATGACCAGATGTCAATGAACA CCCTGACCTCCTTCTGTGGGGCCCTGCCACATCCTTTGTTACGCCTAGCTTCCCCACTGA CGCAGATGTGCAGTTTGTCATCCAGATGCGCCCAGCCTTGAAGGGCGCTATTCTGAGTCT TCTGGGTCATTACAAGTGGGAGAAAGTTTGTGTACCTCTATGACACAGAAACGAGGATTT TCCATTCTNNCAGCGATTATGGAAGCAGCAGTGCAAAACAACTGGNNCAGTACAGCNAGG GTCTGTGGGAAACATAAAAGGACGTNCCAGNATTCANGCGCATCATTGNAG
Restriction Sites	NotI-NotI
ACCN	BC032004
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC032004.1, AAH32004.1
RefSeq Size	1011 bp
RefSeq ORF	1011 bp
Locus ID	2892
Protein Families	Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
Protein Pathways	Long-term depression, Neuroactive ligand-receptor interaction
Gene Summary	Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing at this locus results in different isoforms, which may vary in their signal transduction properties. [provided by RefSeq, Jul 2008]

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