CBL (AK092300) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C-CBL; CBL2; FRA11B; NSLL; RNF55
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK092300, the custom clone sequence may differ by one or more nucleotides TCAGGACCCAAAGATCCTTTAGTCAGTTGTTGGGTCTTCCAAGAGCCAGACATTAATACAGATTGAACTC CATCAGTCCCCTAATTGTCAGCCTTTACCTCCCTCCCAGAGCAAGGAGTTTAGGGATTCTAAAGCTTAGT GTCCACACATCATTCTACCAGACCTTAGAGCTTTAGAAGCTCAATCTAAAATACTGTAACTCAGCATAAA CTATTACTATCACTCCTTTGAACTCAGTCTCCATGAGCAGTGTTTTGTTGGAAATACATAGAACGGCTTA ATGCCTAGAGGGTGGTGGATAGTGAAGGACGGTCAAGGTTATATTTTTGACTGCTTAGGGATTCTTTGGA TACAAGAAACAGAAATGTTCAAGCGGAATAAAGGAGGGAGTGGAGTTGTGGTAAGGATGCAGGGTATTTC GCAGAACCCAGGACGGGAAGTGCCTTTGGTTCTTGGGTGGAGCTGGAACTGCAGAGCTTTGCACCTAGTC CTTTCTCCCGCTTCACAGTCTGCTTATGGTATATGTGGCCCCCAAATAGGCACTCTAGTCCTCAAGTCTA CACCACCTTCCAACTCTGGGGATCACCATGAACAAATTCTCAATTTCCCATACTTAATTTTTTTTTTTTT TGAGATGGAGTCTCGCTGTGTCGCCCAGGCTGGAGTGCAGTGGTGCAGTCTCAACTCACCACAACCTCCG CCTCCCAGGTTCAAGCAGTTCTCTGCCTCAACCTCCCGAGTAGCTGGGATTACAGGCGCCTGCCACCATG CCCAGCTAATGTTCATATTTTTAGTAGAGACAGGGTTTCACCGTCTTGGCTAGGCTGGTCTTGAACTCCT GACCCTCATGATCCACCCACCTCGGCCTCCCAAAGTGCTAAGATTACAGGCGTGAGCCACCGCGCCCGGC CCATACTTCGTATTCTTAAAAAAAACTACACTCAGCCCAGCACATTGATCAAGTATCTATCTCTGAGCAG TTGGCCTTGCCAGGGAGAGCAGAGATGTGGCAGGCTCCTTCAGCTGGAGACAGGGAGCTTCTCAGAGAAG TGAGCAGAGACTCCACAGACACCCTAAAAAGGCTTCTACTCAAGAAGTAAAGCCACTACTCCTGCCTTTT TGCTTAGTGGACAGGAAGGCACAGGAGTTTGTCTGGGACATCATAGAAATTCTTAGGTTTAACTTAATTC TGGTCATTGTCTTCTTTATTTCCTGTTTTTCTTCCCTTTGTCAGTCTTCGCATCCAAGATTTCTTCCCTC CCTCTTGTGGGCCAGCCTGTCCTGTTCCAGAGCTAGCCTGTTCCTGGGTAGCCTTCCTTAGCCTCCATTC AGCCTCAGGTCTTTTGCCTTCTTCCGTGTTTATTTAGAGAGCAGAATCTAATAACGGGTTCCACTGTAGC CACTATCCATGGACTTCTGGGTCCTCTTCAGGTTTGAGTGCTTGAAAATGTTCATTCTCTGGGCTTGTGG CCTGTCTCCTCCACTCTCCTCCTCACCCTCTCGCTCCTTCCTGTGTGAGGGCCGCTCTGCAGTAATGTTC TCAGGCAAGCCTTCCTAGGCACCTCAGAAACTACTTTGCCAGAGCCAGTAAGAATATATAATATTGGAGC AGTTGCCAGGATAGAAATTAAATATAGATTCCAGTTTAGGATAGAGTTTTTACCGAGAGCTCTTTAGACA GTATACCTGTGTCTTCTCTGGCAATTGCTTTCATTTTAGTCCTATATAAAAGCTTTCCTTTTCTGTTTTT TTTTAAAACTATGCTTTTGCTTGCCTAAATCTTTTGATCTTATATTTCTCTCATCTCAGAGCCTGTCCTG AGTTGTAAGGTATTTCATACTGCCTTACTTAAAAGTTTTTTAAACTACTAGAGTCATTTGATACACACAG AAGTTACCTAATAATCCAAAGATGTCCATCAAGGGAGGAAGGGTGGGTCATCAGACTTTGCCTTTGATGT TGTAGACTAGGCTCCTGAGTTAAGCAGCAGAGGGACAGCAGTGCCATGTGCCTTCACTGTGTCCCAGGAA ATCTGGGTTGGTTCCAGTGGGAAATACCAGTATTTCTTGGTTCTGGAAAGTAGCAAAAGAGTAGGAGATG GGGAAATAGGGATGGGGAGAGCAAGCCCCGCATGTCCATGGCGAGTCAGGTGGGGAGCACGGGTGGAAGG GCCGGCTGTTGACAGACAGACTAAGCTGTGTGGTGCTCTTGCCGCCCCTTCCTGGGTACAGAGCTTGAGA AAAATGCAGCCGACCACTCCCTGTGTTTGTACAGAGCAAAGCCCAAAAGCCAACCTCAGATCTCCTGATT TGGCAGCTGAAGAAATCAGCAGAGTCCTGATTGCCTGATTCAGTCCCAAAAATGAATGTCAGGCCCCGCC CCCTCCCCACCAACATTGCCTCTCCTACATTCTCCTTCTGCCCCTAAATCAGACAGGAGGCCAGAGAGGA GTATTGCTCAATGCGTGCTATGTGCAACTCCTCAGGCCTTGTGCCACCTCCATGCTGAGCCCTGAAGCAG GGTGTCCTGGGTGCCTGTGTGTCAGCTCCCTCCTCTCTACCTACCTCTGACCTTCTTGTGGGTGAGGGTG GCCATGCTTATGGCCATCTTAAAACTGGAGAGGCAGAGAACTACTTATGAGTCTGTAGACCACGTGTTGT CTTCCATGGCCTGTTTCTCCTGCTGTCTGGGTGAGTGAGCCTGCAACGCAATGCCCATGAGAGTAAATGC CTCCTGACCTACCCTGCTCAGCACTGTTCTAGTGTCTTGGCCTTGAAAGAAAAGCCTGACTTCCTGCTGA CACATGTGGTAGGGGCATGGCAGCTATGAGGCACCTCCTACGTCTGTTTTCTGGCTGTGGTGACTTGGGA TTTTTAACCTTATATATCTTTTTCCTTTACTCAAAACAAAACAATTTTTAGCACACTG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK092300 unedited TCAGATTTTGTAATACGACTTACTATAGGGCGGCCGCGAAATTCGCACGAGGCTTTGATA ATGCCTGGGAGATTCCTTGGTGTAAGTGTCATGGATACCGACTGTTTTTATGTTGGAATT TGTTCCAACATAATTAGAATCTGTTTGGTGAGTTGAAAGGTAAGTTGGCTCAGAGTTGCA CAGTAGGGCATTAAATGTTTAAGCAAAGCATCTGCCCACACTCCCCTTTCCAATCTAGTG CCTTCCTTGAACTTTTTCCTGAGCTGCTACGTCCCTAATCCCCCTTGTTGGGAGGATTTT CGTATCACCCTTATGGGACCTGTCACCATGTCCTGTACTATTTGGAATTGGTTTTCCAGT CTTTCAACAACCGTTGTGGCTAACTATGTTTTAGAAGGGCTGGAGGTGTGGGCCCTGTCT TCGGGTCTCAGGACCCAAAGATCCTTTAGTCAGTTGTTGGGTCTTCCAAGAGCCAGACAT TAATACAGATTGAACTCCATCAGTCCCCTAATTGTCAGCCTTTACCTCCCTCCCAGAGCA AGGAGTTTAGGGATTCTAAAGCTTAGTGTCCACACATCATTCTACCAGACCTTAGAGCTT TAGAAGCTCAATCTAAAATACTGTAACTCAGCATAAACTATTACTATCACTCCTTTGAAC TCAGTCTCCATGAGCAGTGTTTTGTTGGAAATACATAGAACGGCTTAATGCCTANAGGGT GGTGGATAGTGAAGGACGGTCAAGGTTATATTTTTGACTGCTTANGGATTCTTTGGATAC AAGAACAGAAATGTTCAAGCGGAATAAAGNAGGAGTGGAGTTGTGGTAGGATGCAGGGTA TTTCGCAGACCCAGACGGGAAGTGCCTTTGTCTTGGTGGAGCTGAACTGCAAGCTTGCAC TAGTCTTTCTCCGCTACAGCTGCTATGGATATGTGCCCCAAAGCACCTATNCTCAGTTCA CACTTCATTTGGG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK092300 unedited GCTCATTTATGNACCGCGGCACGCAATTCTNANGATCGAGTTTTTTTTTTTTTTTTTTCA GTGTGCTAAAAATTGTTTTGTTTTGAGTAAAGGGAGAAAAGATATATAAGGTTAAAAATC CCAAGTCACCACAGCCAGAAAACAGACGTAGGAGGTGCCTCATAGCTGCCATGCCCCTAC CACATGTGTCAGCAGGAAGTCAGGCTTTTCTTTCAAGGCCAAGACACTAGAACAGTGCTG AGCAGGGTAGGTCAGGAGGCATTTACTCTCATGGGCATTGCGTTGCAGGCTCACTCACCC AGACAGCAGGAGAAACAGGCCATGGAAGACAACACGTGGTCTACAGACTCATAAGTAGTT CTCTGCCTCTCCAGTTTTAAGATGGCCATAAGCATGGCCACCCTCACCCACAAGAAGGTC AGAGGTAGGTAGAGAGGAGGGAGCTGACACACAGGCACCCAGGACACCCTGCTTCAGGGC TCAGCATGGAGGTGGCACAAGGCCTGAGGAGTTGCACATAGCACGCATTGAGCAATACTC CTCTCTGGCCTCCTGTCTGATTTAGGGGCAGAAGGAGAATGTANGATAGGCAATGTTGGT GGGGAGGGGGGCGGGGCCTGACATTCATTTTTGGGACTGAATCANGCAATCAGGACTCTG CTGATTTCTTCAGCTGCCAAATCAGGAGATCTGAGGTTGGCTTTTGGGCTTTGCTCTGTA CAAACACAGGGAGTGGTCGGCTGCATTTTTCTCAAGCTCTGTACCCAGGAAGGGGGCGGG CAGAGCACCACACAGCTTAATCTGTCTGTCAACAGCCGGGCCTGCCACCCGTGCTCCCCA CTGACTCGCATGGACATGCGGGGCTTGCTCTCCCATCCTATTTCCCATCTNCTACTCTTT GCTACTTTCA
Restriction Sites	NotI-NotI
ACCN	AK092300
Insert Size	3500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK092300.1
RefSeq Size	2928 bp
RefSeq ORF	2928 bp
Locus ID	867
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Chronic myeloid leukemia, Endocytosis, ErbB signaling pathway, Insulin signaling pathway, Jak-STAT signaling pathway, Pathways in cancer, T cell receptor signaling pathway, Ubiquitin mediated proteolysis
Gene Summary	This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia, and expansion of CGG repeats in the 5' UTR has been associated with Jacobsen syndrome. Mutations in this gene are also the cause of Noonan syndrome-like disorder. [provided by RefSeq, Jul 2016]

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