SNAP25 Interacting Protein (SRCIN1) (AK096097) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	P140; SNIP
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK096097, the custom clone sequence may differ by one or more nucleotides AGCCCCCCAAGGCCCCCCACGGCCAGAAGGCAGCCCCCCGAACGGAGCCCAGTGGGAGGAGGGGCTCAGA TGAGTTGACCGTGCCCCGATACCGCACAGAGAAGCCCTCCAAGTCGCCCCCACCGCCCCCTCCCCGCCGG AGCTTCCCCTCCTCCCATGGCCTGACCACCACACGTACCGGAGAGGTGGTGGTCACCAGCAAGAAGGACT CGGCCTTCATCAAGAAGGCTGAGTCCGAGGAGCTGGAGGTGCAGAAGCCCTAGGTGAAGCTGCGCCGGGC TGTGTCTGAGGTGGCCCGCCCAGCCTCCACACCACCCATCATGGCCTCGGCCATCAAGGACGAGGATGAC GAGGATCGCATCATCGCAGAGCTAGAGGTGTTTGAGAGAAGCTCAGTGTCTTCCCTCCCCCCCACGCCCC GCCGCCAGCTGATCCCCACCTTGCTGTCCCCCCAGGACCTGGGGCCCCCCGGGGGCTCAGCCCCAGGCCC TACACGGAAGAGTGGCGGAGGCAGTGTACCACCCATGAAGGTGGTGACTCCGGGGGCCTCTCGGCTGAAG GCGGCCCAGGGCCAGGCGGGCAGCCCCGACAAAAGCAAACATGGCAAGCAGAGGGCCGAGTACATGCGGA TCCAGGCCCAGCAGCAGGTCTAATGAGCAGGCGCGGAGGCTGTGTGGAGAGTGGACATACCTCACCTGTG GGTGTTTACTGCCCTTGTGGCCTGGTCAGAGGCTGCAGGGTGGCTCCTGGACCCAGATGTTGTGAGAGAC CCTTAGTGCACTCGTTTGATTTAGTTAATATTTATGAGCAGCTCCTGTATGACAGGCCCATTCTGGGTGT GAGGGTGGGGGCTGTGATATGTCCAGATGCTCTCACTTAAAGAAGGTTCTAGACTCAGAGGCAAGACGTT AGAGAAGGTTCTAGACTCAGAGGCAAGAGGTTAGAGATGGGTGAGTCCATCTCTTTTTCAGATGAGGGGG CAGCTCAGAGGAGGAGAGGGGCTTCCCAAAGGTGCACAGCAAGGTGGCATCACAGCCAGGATCGGGACCT AGGAATCTTGGAACCCTTCCCTGCACTGGCCCTGGGACAGCCCTGGGCACCCAAGGCCCTGCCTCCCTAC TGGCTCCCAGGAGAATTTCTCAAAAAGCAGCCCTCCAGCCCCCCGCAGCCTCTCCACAGCTCCCACCTGC CTCCTCATGGCCCCCAGACACCCTGCGAGCCCCACCTCCAGATCTTTTTCCTGCCACTGCCAGGGATGAG TTCCCCTTGTCCTGTCCGGGCTGGACCCTCCTGGGTGCCCAGCAGAGCAGCTGACACTGACAGAGCTTTC GCTGGGGCCAGGCATGCGCATTATCTCATCTCACACTCACCACACTGAGGAAGGTCTATTACCCCCATGT GACGGGGGCGGAAACTGAGGCTTAATCCAAGGCCACACGGCTAGCAAGTGGGGGGCAGGACACAAGTCCA GACTGCCTCCCTCCCCACCACAAAGTGCTTCAGCCTCTCCAGCAGCCCCTTCCCTCAGCTCTAATGATGT TGCACTGTTTCCCACCCTAGTCCCTCCCTCCCCAGAGCCCAGCTCCTCCGCTGTGGTGTCAGCCTCATGC CTTTATGGCCCAGCCTCCACCCCATCTCCGGGGTTGCCTGGGTCTCCAGTGACATCGAGATGGGCTCTTT TCACCCCCCTCCCCCACTGCTCTGCAGCATGTGGCCTTGTAGACCCCCCCCACACCTGCATAGGGCTGTG CAGACCCTCTGTTCCTCAGCATCCTCTCCCCAGCCTCCACCTGGAGCCTCTGGGCTCCTTTCTGGGTGAC TCCCTCTATTTTTTGAGACGGAGTCTTGCTCTGTCTCCCAGGCTGGAGTCCAGTGGTGTGATCTCAGCTC ACTGCAGCCTCCGCCTCCTGGGTTCAAGTGATTCTCGTGCCTCAGCCTCCCGAGCAGCTGAGACTACAGG CTAATTACCACACTGGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACCATGTTGGCCAGGCTGGT CTCAAACTCCTGAGCTCAGGTGATCCTCCCACCTCAGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCC ATTGCACCCTGCCTGGGCGACTCTCCTTTGCCTGCCTCTTCCCTTCCTGTGCCCCACTCCTCAGCCTCTT CTCCTCACACCATGCCTTCCTCTCCTCAGGCACCAGGTGCACTGCTTAGTCCCTGCCCCCACCCAGGTCT CCATTCTGAGCTCCAGTCACCCACACACGCTTCCCCGGGCATCCCCAGGCTCCTCGAATGCGTGTGTCCA GACATAATGCTCCCCCGACCACGCTGCCCCAGCCGGTACGAGGCTGCCCCAGCTGCTGTCTCCCTCAGGA AGGGTCGCCCACCCCCAGCCTTCCCAAGCCCCTCCGTGCCCCTGTCTCACTTCCAGTGCTCCAGTAGCCT TTCTTATAAACATGGCTTCTTTCTTGTAAAGTAATAAATGTTTACAGTGG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK096097 unedited GTAATACGACTCACTATAGGGCGGCCCGCGATTCGGCACGAGGCTGCTATTCAGGGCCCC ACTCTCATCCTGGTCTCCCTCTTAGCTCTTAGGCCACCCCTAGCCAAGGGAGAAGCCAAC TTTGCATCTGCTTTTCAGCCTTGTGTTTGGGTTTCAGTGGGGTCACCTCCCGTCTTTGCA AGGTTGGGTGGGGACAGGGCGAGGCACTTTGGCCCTCAGGAAGGGTAGGGGTGCAGCAGG AATTGCAGGGATGCCGCAATCTCTTTCAGGTCTAATGAGCAGGCGCGGAGGCTGTGTGGA GAGTGGACATACCTCACCTGTGGGTGTTTACTGCCCTTGTGGCCTGGTCAGAGGCTGCAG GGTGGCTCCTGGACCCAGATGTTGTGAGAGACCCTTAGTGCACTCGTTTGATTTAGTTAA TATTTATGAGCAGCTCCTGTATGACAGGCCCATTCTGGGTGTGAGGGTGGGGGCTGTGAT ATGTCCAGATGCTCTCACTTAAAGAAGGTTCTAGACTCAGAGGCAAGACGTTAGAGAAGG TTCTAGACTCAGAGGCAAGAGGTTAGAGATGGGTGAGTCCATCTCTTTTTCAGATGAGGG GGCAGCTCAGAGGAGGAGAGGGGCTTCCCAAAGGTGCACAGCAAGGTGGCATCACAGCCA GGATCGGGACCTAGGAATCTTGGAACCCTTCCCTGCACTGGCCCTGNGACAGCCCTGNGC ACNCAAGGCCCTGCCTCCCTACTGGCTCCCAGGAAATTNCTCAAAAACAGCCCTTCAGCC CCCGNAGCCTCNCACAGCTNCCACCTGCCTNCTCATGGCCCCCAGACACCCTGCGAGCCC CACCTCCAGATCTTTTTCCTGCCACTGCCANGNATGAGTNCCCCTTGTCCTGTCCNGGCT GNACCCTNNCTGGTGCCAGCANAGCAGCTGCACTGACAC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK096097 unedited TACATCTATGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCCCTG TAAACATTTATTACTTTACAAGAAAGAAGCCTGTTTATAAGAAAGGCTACTGGAGCACTG GAAGTGAGACAGGGGCACGGAGGGGCTTGGGAAGGCTGGGGGTGGGCGACCCTTCCTGAG GGAGACAGCAGCTGGGGCAGCCTCGTACCGGCTGGGGCAGCGTGGTCGGGGGAGCATTAT GTCTGGACACACGCATTCGAGGAGCCTGGGGATGCCCGGGGAAGCGTGTGTGGGTGACTG GAGCTCAGAATGGAGACCTGGGTGGGGGCAGGGACTAAGCAGTGCACCTGGTGCCTGAGG AGAGGAAGGCATGGTGTGAGGAGAAGAGGCTGAGGAGTGGGGCACAGGAAGGGAAGAGGC AGGCAAAGGAGAGTCGCCCAGGCAGGGTGCAATGGCTCACGCCTGTAATCCCAGCACTTT GGGAGGCTGAGGTGGGAGGATCACCTGAGCTCAGGAGTTTGAGACCAGCCTGGCCAACAT GGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCAGTGTGGTAATTAGCCTGTAG TCTCAGCTGCTCGGGAGGCTGANGCACGAGAATCACTTGAACCCANGAGGCGGAGGCTGC AGTGAGCTGAGATCACACCACTGGACTCCAGCCTGGGAGACAGAGCAAGACTCCGTCTCA AAAATAGAGGGAGTCACCCCGAAAGGAGCCCAGAAGCTCCCCAGTGGAGCTGGGGAGAGG ATGCTGNAGAACANAGGGTCTGCACAGCCCTATGCANGTGTGGGGGGGGGTCTACAAGGC CACATGCTGCANAACANTGGGGGAAGGGGGTGGAAAAAAAACCCCTTCTTGTTTTCCTTG GGAGACCCGGGCAACCCCCGAGATGGGTGGGGAGGCTTGGC
Restriction Sites	NotI-NotI
ACCN	AK096097
Insert Size	2000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK096097.1
RefSeq Size	2500 bp
RefSeq ORF	2500 bp
Locus ID	80725
Gene Summary	Acts as a negative regulator of SRC by activating CSK which inhibits SRC activity and downstream signaling, leading to impaired cell spreading and migration. Regulates dendritic spine morphology. Involved in calcium-dependent exocytosis. May play a role in neurotransmitter release or synapse maintenance.[UniProtKB/Swiss-Prot Function]

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