Semaphorin 3E (SEMA3E) (AK021753) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK021753, the custom clone sequence may differ by one or more nucleotides ATAAATTAAACTTTATCATGGGTATGTATATAGGAAAAAATAATAGTTTGTATAAGGTTCGAATAGTTTG TATAAGGTTCGGTACTATCCACAGTTTCAGGCATACACCGGGGGTCTTGGAACATATTCCCCTCAGATAA GAGAGAATTCCTGTGTATGGAAGAGACTCCTCAGATACAGCTTCTCTTCAACTGTAAACCTATGAATTAA AAAAAAGTTATTGGTCCTATCCACCCCCGCACATACAACCTACATTGTTATGGCAAGGATACGATGTCAC ATGAATTGACTAAGTTTACAAGAGAGGAAATTGAAGGCATGTAGCAATCCCATGGCAGTTGTGAAATCCA TCTGCCTATATGTCACCAATTCCCCCAATTCCAGGGGTAGGGAACATTTGATTAGTCTACTTTGGTTCTC TGAAGTTGGCTCCCTTTTCTTTTTCTCAGTTCTTGACTTTTTTCTTTGAGCTGTCTTTCCTTTTCCATGA GAAATGTCCTCTTTTTGTAGCTTTCTCAGCCTGCTTCTAGGCTCTGTCCCAACTGGCACAGTTATCCACA CTGGCACAACTTCTTTAAAAAGCTTTGTGGACTTTCAAATTATAAACCACTCACTCCACCAGAGAGAAGC CACACCCACAAATTTCTTCAAGAAGTCCTCTATGTACTTTGAATGTCAATCAGGGAATGATACCCTTTAG AGTCATATATGTCTTTTGTCTACCTGAGAGCGTCAGCTAGACACTGGCTTAAATCTTTCTGAAGTACAGG TGGTCGTCCACTTATGATGGTTCAACTTAGAATTCTTTTACTTTAGGATGGTATGAAAGCTATATGCATT CAGTAGCAACCATACTTCAAGTACCCATACAACCATTCTATTTTTTACATTCAGTACAGTATTCAGTAAA TCATGAAATATTCAGCACTTCATTATAAAATAGGCTTTGTGTTAGATTACTTTGTTCAATATAACATAAT GCAAGTGTTCTGAGCACATTTAAGCAATGACGGGTTAAGCTGTGATGTATGGTAGGTTACGTGTACTATT CAACTTAATATTTTCAGCTTACGATGGGTTTGTCAGGACATAACCCCATTGTAAGTCAGGGAGCATCTGT AGTAGTAACAAGGTTGTATTGCATGTGCTTTATTTTATCTTGATCCTCAGACCATAATCTTACAGTTAAC ACCCTGGATTTTTTTTTTTTTAACTTCAGAACCTTTTGCTGAAGAAGCTGGTAACGAGAAAGTTTTATTT TGTAACCCTGCAAGTCCCAGGTTGAAAGTATTTTCCTCTAAATTCTGCTTGAAACTGAGCAGTTCCTTGT TTAGTTCTTCTCTCTTTTAATACCTTTCTACAGGTGTTTTTTGAAAAATTGCTTATCACTTTCAGCATTT TTCCTGGAAACCTTAGCCAGATCTATAACTTCAATAGGTACTTTTTCTATCTTCCAAGATACTGTCTCAC TTGTTTTGTCAGTAGATTACATGGCTTCTGTCCAGCCTGAAATACCAATTTCCTCAGTGGTTTTCCAGCC TCCGTTAGTAGTCTCTTTGCTGCTCTTCCACCAAATGTCTATAACCCAGTCCCCAAACTAGTGCTACATG TCTTAAGTTTCTGTCATGGCAATGCCCTGTTTAAATACCAAATACTATTTCAGTTATCTTTTTCTGCCTA ACAAATCACCCCAAAATTTACTACCTTAAAACAAAACTATTCTTTGCTTGAGATTCTACTGTCTGAACTG GACTCAGCTGGGCATTTCTTCTGGTCTCACCTGGAGTCATTTATGCAACTGCAGACATGTGGGGACTCCA CCAAAGATGGCTTTACTCAAATGTCTGGGGCCTCAACTGGGGTGGCTGCATTAGCTCTGGCACAGCTGCA GTTCCCTCTCCAGCAGGGTCCTGGGCCATTTCACATGATGACTGAGGGCTCCAAGAGGGTGAAAGCAGGA GCATCTGGGCTAGGCCTCTTAGAGCCTGTGCATAAAACTGAAACAGCACTACTTCATCCATGCTGCCTTT CAAAGCAAGTCCCGGGGCCTGCTCAAAATTACAGGCAGGGAAAATATCCTCTACCTGATGGTAGTGACAA AGAATATGTGTCCCATCGTTAATTCACCAGTTGCCTTCACAGTTGCAACAATGAGACTGTTAGCTTTTAA ACAGAAATGATAAAAACTAGAAGCCGGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAG GCCGAGGCGGGTGGATCAGGAGGTCAGGAGATTGAGACCGTCCTGGCTAACAAGGTGAAACCCCGCCTCT ACTAAAAATACAAAAAATTAGCCGGGCGCAGTGGCGGGCGCCTCTAGTCCCGGCTACTCGGGAGGCTGAG GCAGGAGAATGGCGTGAACCCGGGAAGCGGAGCTTGCAGTGAGCCGAGATTGCGCCACTGCAGTCCGCAG TCCGGCCTGGGCGACAGAGCGAAACTCCGTCTC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK021753 unedited TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATCTGCCTGGCTTC TCAAAGCCTAGAAACTCATAGAGCATCATTTCAACTGCATTCTTGTTGGTCAGAGCCAGT CATCACAAAGCCAGCTGAGATTCAAGGAAACAGATAGAACTTCACTTCTTGATAAGACAT GGGTGAAGAGGAGGGCAGATAGAATTTTAGGGCATCTCTCATTTGCCTGAGTCTTCCTAC TGGCTCACATTGCTTAAATTCCTCCGACATGCAAAATGACACCCACCCCAAGAACCCCAC AGTCCCATCCAATTATGGCATCAGGCTCAGAGTCTACTTGTGTACAGTAGTTCCCCCTCA ACTGTGGTTTCGCTTTCCACAGTTTTCAGTTACCCACAGTCAACTGAGGTTCAAAAATAG ATGAGTACAGTATTAATAAGACATTTTGAGGTAGAGAAAGATGCAGACCACATCCACACA ACTTCTATTACAGTGTATTATTTTAATTGTTCTGTTTTATTATTATTAATCTCTTACTGT GTCTAATTTATAAATTAAACTTTATCATGGGTATGTATATAGGAAAAAATAATAGTTTGT ATAAGGTTCGAATAGTTTGTATAAGGTTCGGTACTATCCACAGTTTCAGGCATACACCGG GGGTCTTGGAACATATTCCCCTCAGATAAGAGAGAATTCCTGTGTATGGAAGAGACTCCT CAGATACAGCTTCTCTTCAACTGTAAACCTATGAATTAAAAAAAAGTTATTGGTCCTATC CACCCCCGCACATACAACCTACATTGTTATGGNCAGGATACGATGTCACATGAATTGACT AAGTTTACAAGAGAGGANNNATGAGGCATGTNAGCATCCATGGNCAGTGTGAAATCATCT GCCTATATGTCACCAATTCCCCCATNC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK021753 unedited GTCATTAGNACCGCGCCGCAATCTANGATCAGTTTTTTTTTTTTTTTTTTTTTTTTTTTG AGACGGAGTTTCGCTCTGTCGCCCAGGCCGGACTGCGGACTGCAGTGGCGCAATCTCGGC TCACTGCAAGCTCCGCTTCCCGGGTTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTAGC CGGGACTACAGGCGCCCGCCACTGCGCCCGGCTAATTTTTTGTATTTTTAGTAAAGGCGG GGTTTCACCTTGTTAGCCAGGACGGTCTCAATCTCCTGACCTCCTGATCCACCCGCCTCG GCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGCGCCCGGCCGGCTTCTAGTTTT TATCATTTCTGTTTAAAAGCTAACAGTCTCATTGTTGCAACTGTGAAGGCAACTGGTGAA TTAACGATGGGACACATATTCTTTGTCACTACCATCAGGTAGAGGATATTTTCCCTGCCT GTAATTTTGAGCAGGCCCCGGGACTTGCTTTGAAAGGCAGCATGGATGAAGTAGTGCTGT TTCAATTTTATGCACAGGCTCTAAGAGGCCTAGCCCAGATGCTTCTGCTTTCACCCTCTT GGAGCCCTCAGTCATCATGTGAAATGGCCCAAGACCCCGCTGGAGAAGGAACTGCAGCTG TGCCAAACCTAATGCAGCCACCCCATTTGAGGCCCCAAACATTTGAGTAAAGCCCTCTCT TGGTGGAGTCCCCCCATGTCTTGCAGTTGCCCTAATGAATCCCGGGGGAGACCCAAAAAA ATGCCCACCTGATTCCCCTTCTAACCGGGTGATCCTTAGCAAGGAAATATTTGGTTTAAG GAGTAAAATTTGGGGGGAGTTTGTTAGGCACAAAAAAGAACCGAAAAAATTTTTGGTTTT TAAACAGGGATTCCCCGACAAA
Restriction Sites	NotI-NotI
ACCN	AK021753
Insert Size	3080 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK021753.1
RefSeq Size	2483 bp
RefSeq ORF	2483 bp
Locus ID	9723
Protein Families	Secreted Protein
Protein Pathways	Axon guidance
Gene Summary	Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. This gene encodes a class 4 semaphorin. This gene encodes a class 3 semaphorin. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]

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