Cyclophilin E (PPIE) (AK093659) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CYP-33; CYP33
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK093659, the custom clone sequence may differ by one or more nucleotides ATGGCAGCCCCAGGGTGTGGGGCAGGTGGGCAGGGCACTAATGAGGGGCCTCGAGTCCCAGGCCAAGGGC TCAGACTTCCTCCTGGGGATAATGGGAAGGAGAACCTCATGGCCAGGTTTGTGCCCTTAGTGAGGTCCCC AAGGGGGAACCTGGAAGGACCACTGTCTTCTTATAGCCCTTCAGACCCCCCCAGAGGCTCTGCCTCCCCA CACCCTGGCCTCCCTCCCCAGGCTCTGGGAGGCCAGGCCTCGCCCCCTCTCCTGTGCTGCTCCTTCTCCC AGAGGACTGCATCCGCCCTCAGCACTTCAAGGCCCTGCATGTGCCCGCACCTCTCCACTCCCCTGGCTCA GCTTGGACCTCTCTTCTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK093659 unedited GTAGAATATGTAATACGACTTACTATAGGGCGGCCGCGAAATTCGCACGAGGCTTAGGCC ACCAAAGTGTCGGGCAGAGTGACAGCCACAAACCCCAGGGAGGGGCACAGAGGTACCGAG AAAGGACCAGAAGCCTGACAGGAAGTAGGCACCGAGAGTGAGGGCCCCTGACACTCCTGC TTGAGCTGTGACAGCATTTAAGGGTTTTCTGCCTCTGCAGGGCCCGATCCAGATGAGAAG GGTGGCTGCAGTAGGGCTGGGCGGGCTGACTCAGTGGCAGCCGCAGGCCTTGACCACCAT GTTGCGGTGCTTGCGCAGGATGACGTTGTTGCTGCTGTCATAGTAGAGCACAGAGGTGGC GCTCAGCTTGGTGGGTGCACAGCACGCCTTGGGGACTGCGTTTGGCTTCATCAGGTGCAC CTGGCCAGGAAGGGAGCACAGGCAGGGGCATGAGGCCAGTGGCCTCCTCTGAGGACCCAC CTAGCCCCGCCCCAGCTCCACCTGCTCCCTGCCCTGGCCATCTCCAAGCCACATGGGAGC AGCACAATAATAAGAATTATTAACAGGAATAAGAGCAGCCCTGAGAGCTCTGGGAGCGGG AGCTGTGGTGGCCCACAGTGCCCAGGGAGGGATACATGGCAGCCCCAGGGTGTGGGGCAG GTGGGCAGGGCACTAATGANGGGCCTCGAGTCCCAAGCCAAGGGCTCAGACTTNCTNNCT GGGATAATGGGAAGGAGAACCTCATGGCCAGGTTTGTGCCCTTAGTGAGGTCCCCAAGGG GAACCTGGAAGACACTGCTTCTATAGCCTTTTCAACCCCCCCAGAGCTCTGNCTNCCCAC ACCTGGCCTNNCTNCCAGCTCTGGGAGCCAGNCTCGCCCCTCTCTTGCTGNTCTCTCCAN AGACTGCTNCGCCTCACACTTTAGATCTGCTGTGCCGCACTCTCATTCCTGCTCACTGGA CTTT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK093659 unedited TTTATGNACCGCGCCGCAATCTANNGATCGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTCCGGGCATTCATTTATTTGACAAATGTGCTCATCAGGGCATCCC CCACCCTGGTACCCCATAGTAGCTGCACACAGTAGGAACCCCAAAAAAAACCTTGCCCCT TTTGTCCCTGCAAATGACGTCCACGGTTCCCACGGCCGGCAGGTTTGCCGTCGGCACGAG CTTTACGTCAGCTTCCAGGACCTTGGCTGGCTGGAAATTGCTGACTCTCCTTGTTTCTGG GCCGCACCACAGGCAGGAAGCTTCTAGATGGGGGGGCTCTCAACCTTTTGGCTTTTTCCC GCAGTTTCTCTCTTGGCTGGCTGGGTTTTTTCTGGATCCCCTGGCTTTTGATGCCTTGGT GTCTGGGGCAACCTAAAATCCCAGTTCCTGGTATTGCACAGCCTTATGTTTTCCTCTTAG GAGGTTCAACGGTGATGCCTTGATCAGGCGCAGTGGCTCACCCTGTAATCGCAGCACACG AGCCCAGAAGTTCAAGACAAGCCTGAGCAACACAGCAAAACCCTGTCTCTAAAATAAAAA TTAAAANANANACNCNCNCNCNCACACACACACACGTGCGCACACAATGCCTTGGTGTGA GAGGAAAGAAATTACCAAAAGCTGCTCTGAGCCTATGATAATACTTCCTTTCTGGGCAGT CAAATGGTGTTTGCTGGACACCTGGAGCCATCTCCTTGGAAAGGCCCAGGGGTGATGAGG ACCCGTGGGGTTGGCCCTGCCCCANCCACCCTTTATGCCNGTGTGGTTCTCACAGCTGCA TGTGTGGGGAGGTACATGGGAAGGTGAACTGCCCTGCAGCTCCCGGAACTCATTTCCTCT GCCCTTCCCCTGGCCCTAAGGGCTAACTAGAGGAGTGCTCCAGCCTACAGGGCACAACAG GCCTGTGTCTAGGGN
Restriction Sites	NotI-NotI
ACCN	AK093659
Insert Size	2700 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK093659.1, BAC04213.1
RefSeq Size	2235 bp
RefSeq ORF	2235 bp
Locus ID	10450
Protein Families	Transcription Factors
Protein Pathways	Spliceosome
Gene Summary	The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities, and it also exhibits RNA-binding activity. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on chromosome 1, has been identified. [provided by RefSeq, Aug 2010]

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