RPH3AL (AK026755) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK026755, the custom clone sequence may differ by one or more nucleotides TTTTGCTTATATTTTGGAGGGGTTGTCTTTTTCTTAACGATTCGTAAGAGTTCCTTACGTATTCGATCAT TTTGTATTTTTGTGGGTTACGTATCTCTTTGTAGCTTGTCTTTTCACATTAGGGCATTTGATGTAGATTA AAAATTTGTATATTAACAGAATCTTGATATTTATCTTCATAGTTTGTGCTTCTTATGTCTTGATTTGTAA TGACATCTCTATATTGGGTTTTCATAGATTCATGGGGTGCTAATGTTTAAATCTGTAATTATAACTATTT TTAGTTTCATTATAATTTTTCCCTATTTAATTCAGTACTTTATTTCCCCCACTACCCCCCACCCCCCAAG AGACGGGGTCTTCCTTTGTTGGCCAGGCTGAAGTGAGTGCAGTGACACGATCACAGCTCACTGCAACCTT GAACTCCTGATATCAAGTGATCCTCCCACCTCAGCCTCCAGAAGAATTAGGAGTACAGGCACGTGCCACC ATGTCTGGCTTATATATATTTTTGGGGGGTGGTGGGGGAGAGTAGAGATGAGGTTTCGCTATGTTGTCCA GGCTGGTCTCGACCTCTTGGGCTGAAGTGGTCCTCCTGCCTCAGCCTCCCAAAGTACAGGATTGCAGGTG TGAGCCACCACGCCTAGCCCTTTTATAATTCCTTCTATTTGTTCATTCATTCAACAATTCAGTCAGAAAT ACTGAGTGCCCATTACACACTAGATATGCTGCTAGGTGCTGGGATTACAGGCATGAGCTACTGTGCCCAG CCAGTTTTTGGTATTTTTAGTAGAGACGAGGTTTCACCATGTTGGCCAAGCTGTTCTTGAACTCCTGACC TCAGGTGATCCACCTGCCTGAGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCATGCCCGGCCGA TAAATTTTTTAAAATCTTCTTTCTTTATACATGTTTCTGTGCTCTGTTTAGACAGTCTCATAGATATATT TATTTGTTACGAATTGTCCCTTCATCAGGAAAATGATTCCTTTTTCCCTATTTAAGGTATTTGTTACGAA TTCTAGTTTTATATTATCTACTATATCTTACCCTTAACTTTTCTGAGTCATTTTCAGTGTATCTTTTATA GAAGGCTATGGTTGGAATTTTTTTAAAACCTGAGTATCATTGTCTTCTGATACAGAATCTGAAAACCTTT TAAAAATTAGTGAGGCGAAAAAAATTAGCCAGGCATGGTGACGGGTGCCTGTGGTCCCAGCTACTCGGGA GGCTGAGGCAGGAGAATGGCACGAACCCGGAAGCCAGAGCTTGCAGTGAGCCGAGATCGCGCCACTGCAC TCCAGCCTGGGCGACAGAGTGAGACTCCGGCTCAAAAAAAAAAAAAAAAAAAAAAAGTATTCAGGCTGGG ATTACAGGTGTGAGCCACCGCACCCAATCCTATTAGGTTTCTTTGAATCCCCTCATGGCCTGCCTGGTTT TTGCTCAGCCTGTCTTCAGCTTGAGGAGCTGGGAAGCTCTGGTGGATGCTATGAACTCACTTGCTGAAGA GCAGCGTTCAGGTGCATCCCCAGCCAGGGCACGTGGCTCCCTCAGCCATGAATTCACTTCTCTTCAGGAG GTTTGGCTTGGCATGAAAATACTTCATTCAGAGTATGGGCAAATGCTTCTGGAAAACCCATCCCTGAAGA GAGAGAACGTGTGTGTGTGTGTCGGTGATCACACCCTCCCATCCTTCCTGCCTCCTGCCCCAAACCCCGG GTTCCTGGGTCTGGAAGGGCCTTCTCTCCAAGCTGGGAGCTCCTGGGCCCCCACCATTCACTTTTTGTCC TTGCTGCTGGCAAACAGTAAAGAAACTCACTTTCCCTGTGGCACGTTATGCTTCAGAATTAAAACAATGA AGTATTAAAATTTAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK026755 unedited TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCACATGCAGTGTCAGAGGG GCCGCCCGGTGGGGCTATCTCCGTTGCTATATTAATGGCAAGACTAAATGAAACCTAGGG CACGGCCTCCGAAGCTGCGTGTGGCCCCTTAGAGGTGAGCATCAGAGCCAGAGCAGTGAG GGGGAGACTCACCCACCCTCTCCCTCTCCCTTCAGCTCTGGGAGGCAGGCGCAGTGCCCC CCTCCCGTGGGCTGGCCCAGGACCGCGGGTGAAACCTGGGTCTGTTTAGTTTCTTTGGTT TTTGTATGTTTGTTTGTTTTTGACACAGTCTCGCTTTGTTGCCCAGGCTGGGGTGCAGTG GCACGATCGCGGCTCACTGCAACCTCCACCTCCCGGGCTCAAGCGATTCTCTCACCTCAG CCTCCTGAGTAGGTGGGATTACAGATGCCCGCCACCACACCCAGTTAATTTTTGTATTTT TAGAAGAGATGGGGTTTCTCCATGTTGGCCAGGCTGGTCTTGAACTCCTGGTCTCAAGTG ATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCGCACCCAAT CCTATTAGGTTTCTTTGAATCCCCTCATGGCCTGCCTGGTTTTTGCTCAGCCTGTCTTCA GCTTGAGGAGCTGGGAAGCTCTGGTGGATGCTATGAACTCACTTGCTGAAGAGCAGCGTT CAGGTGCATCCCCAGCCAGGGCACCGTGGCTCCCTCAGCCATGAANTNCACTTCTCTTCA GGAGGTTNGGGCTTGGCATGAAAATACTTCATTCANAGTATGGGCANATGGCTCTGGNAA AACCCTTCCCTGANAGAGAAACGTGTGTNGTGTGTGTCGGTGATCACACCCTTCCATNCT TTCTGNCTNCTGCCCAAAACCNNGGGTTCTGGGTCTGGAAGGGCCTTCTCTNNCAGCTGG GACTCCTGGGGCCCACCATCACTTTTGN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK026755 unedited NNCGGTACTACTATGNNACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTT AATCTTCATTGTTTTAATTCTGAAGCATAACGTGCCACAGGGGAAAGTGAGTTTCTTTAC TGTTTGCCAGCAGCAAGGACAAAAAGTGAATGGTGGGGGCCCAGGAGCTCCCAGCTTGGA GAGAAGGCCCTTCCAGACCCAGGAACCCGGGGTTTGGGGCAGGAGGCAGGAAGGATGGGA GGGTGTGATCACCGACACACACACACACGTTCTCTCTCTTCAGGGAAGGGTTTTCCAGAA GCATTTGCCCATACTCTGAATGAAGTATTTTCATGCCAAGCCAAACCTCCTGAAGAGAAG TGAATTCATGGCTGAGGGAGCCACGTGCCCTGGCTGGGGATGCACCTGAACGCTGCTCTT CAGCAAGTGAGTTCATAGCATCCACCAGAGCTTCCCAGCTCCTCAAGCTGAAGACAGGCT GAGCAAAAACCAGGCAGGCCATGAGGGGATTCAAAGAAACCTAATAGGATTGGGTGCGGT GGCTCACACCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACTTGAGACCA GGAGTTCAAGACCAGCCTGGCCAACATGGAGAAACCCCATCTCTTCTAAAAATACAAAAA TTAACTGGGTGTGGTGGCGGGCATCTGTAATCCCACCTACTCAGGAAGCTGTAGTGAGAG AATCGCTTGAGCCCGGGAGGTGGAAGTTGCACTGAGCCGCGATCGTGCCACTTGACCCCA AGCTGGGCAACAAACGAGACTGTGTCAAAAACAAACAAACTTTCTAAAACCCAAGGAACT TAACCGACCCGGGTTTCACCCCCGGGTCCCTGGGCCACCCCACGGGAAGGGGGCACTGTG CCCTGGCTTCCC
Restriction Sites	NotI-NotI
ACCN	AK026755
Insert Size	1250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK026755.1
RefSeq Size	1917 bp
RefSeq ORF	1917 bp
Locus ID	9501
Protein Families	Secreted Protein
Gene Summary	The protein encoded by this gene plays a direct regulatory role in calcium-ion-dependent exocytosis in both endocrine and exocrine cells and plays a key role in insulin secretion by pancreatic cells. This gene is likely a tumor suppressor. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jun 2010]

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