ATG16L1 (AK027854) Human Untagged Clone

CAT#: SC102544

(untagged)-Human cDNA FLJ14948 fis, clone PLACE2000164, weakly similar to TIPD PROTEIN



CNY 3,760.00

CNY 6,270.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
ATG16L1 mouse monoclonal antibody,clone OTI5A2
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms APG16L; ATG16A; ATG16L; IBD10; WDR30
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC102544 sequence for AK027854 edited (data generated by NextGen Sequencing)
ATGGCCCAACTGAGGATTAAGCACCAAGAGGAACTGACTGAATTACACAAGAAACGTGGG
GAGTTAGCTCAACTGGTGATTGACCTGAATAACCAAATGCAGCGGAAGGACAGGGAGATG
CAGATGAATGAAGCAAAAATTGCAGAATGTTTGCAGACTATCTCTGACCTGGAGACGGAG
TGCCTAGACCTGCGCACTAAGCTTTGTGACCTTGAAAGAGCCAACCAGACCCTGAAGGAT
GAATATGATGCCCTGCAGATCACTTTTACTGCCTTGGAGGGAAAACTGAGGAAAACTACG
GAAGAGAACCAGGAGCTGGTCACCAGATGGATGGCTGAGAAAGCCCAGGAAGCCAATCGG
CTTAATGCAGAGAATGAAAAAGACTCCAGGAGGCGGCAAGCCCGGCTGCAGAAAGAGCTT
GCAGAAGCAGCAAAGGAACCTCTACCAGTCGAACAGGATGATGACATTGAGGTCATTGTG
GATGAAACTTCTGATCACACAGAAGAGACCTCTCCTGTGCGAGCCATCAGCAGAGCAGCC
ACGAGACGCTCTGTCTCTTCCTTCCCAGTCCCCCAGGACAATGTGGATACTCATCCTGGT
TCTGGTAAAGAAGTGAGGGTACCAGCTACTGCCTTGTGTGTCTTCGATGCACATGATGGG
GAAGTCAACNNNNNNNNGTTCAGTCCAGGTTCCCGGTTACTGGCCACTGGAGGCATGGAC
CGCAGGGTTAAGCTTTGGGAAGTATTTGGAGAAAAATGTGAGTTCAAGGGTTCCCTATCT
GGCAGTAATGCAGGAATTACAAGCATTGAATTTGATAGTGCTGGATCTTACCTCTTAGCA
GCTTCAAATGATTTTGCAAGCCGAATCTGGACTGTGGATGATTATCGATTACGGCACACA
CTCACGGGACACAGTGGGAAAGTGCTGTCTGCTAAGTTCCTGCTGGACAATGCGCGGATT
GTCTCAGGAAGTCACGACCGGACTCTCAAACTCTGGGATCTACGCAGCAAAGTCTGCATA
AAGACAGTGTTTGCAGGATCCAGTTGCAATGATATTGTCTGCACAGAGCAATGTGTAATG
AGTGGACATTTTGACAAGAAAATTCGTTTCTGGGACATTCGATCAGAGAGCATAGTTCGA
GAGATGGAGCTGTTGGGAAAGATTACTGCCCTGGACTTAAACCCAGAAAGGACTGAGCTC
CTGAGCTGCTCCCGTGATGACTTGCTAAAAGTTATTGATCTCCGAACAAATGCTATCAAG
CAGACANNNNNNNNNNCTGGGTTCAAGTGCGGCTCTGACTGGACCAGAGTTGTCTTCAGC
CCTGATGGCAGTTACGTGGCGGCAGGCTCTGCTGAGGGCTCTCTGTATATCTGGAGTGTG
CTCACAGGGAAAGTGGAAAAGGTTCTTTCAAAGCAGCACAGCTCATCCATCAATGCGGTG
GCGTGGTCGCCCTCTGGCTCGCACGTTGTCAGTGTGGACAAAGGATGCAAAGCTGTGCTG
TGGGCACAGTACTGA

Clone variation with respect to AK027854.1
200 g=>a;670 g=>n;671 c=>n;672 t=>n;673 g=>n;674 c=>n;675 g=>n;676 c=>n;677 a=>n;1267 t=>n;1268 t=>n;1269 c=>n;1270 a=>n;1271 g=>n;1272 t=>n;1273 g=>n;1274 c=>n;1275 a=>n;1276 a=>n
>OriGene 5' read for AK027854 unedited
GGAATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCCGGCATGAGC
CGGAAGACCGTCCCGGATGGCCTCGGGGACTGCCAGTGTGTGGAGGTGAGCTCCGGGATT
GCCGGCATTCCCGCTTCTGCTGGTTGCTTCATGCTGCAGGCTGCGGCCGTCAGCCCTCGC
TCGCATTGGTGGCGCTGAGGTGCCGGGGCAGCAAGTGACATGTCGTCGGGCCTCCGCGCC
GCTGACTTCCCCCGCTGGAAGCGCCACATCTCGGAGCAACTGAGGCGCCGGGACCGGCTG
CAGAGACAGGCGTTCGAGGAGATCATCCTGCAGTATAACAAATTGCTGGAAAAGTCAGAT
CTTCATTCAGTGTTGGCCCAGAAACTACAGGCTGAAAAGCATGACGTACCAAACAGGCAC
GAGATAAGTCCCGGACATGATGGCACATGGAATGACAATCAGCTACAAGAAATGGCCCAA
CTGAGGATTAAGCACCAAGAGGAACTGACTGAATTACACAAGAAACGTGGGGAGTTAGCT
CAACTGGTGATTGACCTGAATAACCAAATGCAGCGGAAGGACAGGGAGATGCAGATGAAT
GAAGCAAAAATTGCAGAATGTTTGCAGACTATCTCTGACCTGGAGACGGAGTGCCTAGAC
CTGCGCACTAAGCTTTGTGACCTTGAAAGAGCCAACCAGACCCTGANAGATGAATATGAT
GCCCTGCAGATCACTTTTACTGCCTTGGGAGGAAAACTGAGGAAAACTACGGAAGAGAAA
CCAGGAGCTGTCACCAGATGGATGGCTGAGAAAGCCCAGGAAGCCAATCGGCTTAATGCA
GAGAATGAAAAAGAACTCCAGGAGCNGCAAGCCCCGCTGCAGAAAGAGCTTGCAGAAGCA
GCAAAGAACCCTCTACAGTCGACAGGATGATGACATTTGAGGCATTGTGGATGAACTNCT
GATACACAGAAGAGACTCTNCTGTGCGAGCATCAGCAGACAGCCCGAGACCN
>OriGene 3' read for AK027854 unedited
NTTTTNAGGGTATTAGCTAGNNACCGCGGCCGCAATCTAGNGATCGATTTTTTTTTTTTT
TTTTTGAAAAGTTATAACAGATTTCTTTATTATTATTTACAATCAAGTTCTGTTGGCCAA
CATAATGAAATAAATAAAAGATGTGCCCTGGCCTGTGAATTTCAACTCTCCTTGACTTAA
GTTCTCTGAAGGGCAAATTGGAAAGCGGTGATCAGGCAGGGAAGAGAGGGCAGGTGGAGG
CCAGGACCATCGGTGGGAAGGCCACCTGACTCCTCTCTCACCAGCTCTAACACTCACATC
CCCAAATGTCCAGAGAACAAGCATGGAAGAAAAAAAATAAAGTGCAAATTTAAAAGTGAT
AAAAAGGGTGTTTCGCACACCCAATGAACTAAAACTTTATACGTAGGTAAAATAGTAAAG
ATAAATGTTTTTCCTTGGCCTTCATCACAACCCCTGAAACGGAAAGATGGCGCTGCTGTG
CTTCTGAGCCTAGGCTTCTTGCACTAAAGCACCAAGGGCATCGCACACAGGCTTGGCAGA
GGGGCCATGGCCAGAATCACCACCTTCAGACAAGTATGNTTGGAGGTCTCGAATCCCTTG
GCACCCCCAAGCATGCAGTGTGCACCCACCTTCTGGGTGTCTGCAGATCGATACAGGAAC
ACATCCTTACCTCTGCAGCGAACCCATTTTCCTGGAAAAGATTTTCACACATCCTCTCCC
ACCTCAAAAAAACAAAAAAACAAAAACCATTTTTTTAAGCCACACGGAGATTTTTAATAT
GTCCAAATTTGTGGGGGAAAGAGAGCCCGAAAGCTCTGAAGAACGTGAAAGAAATTTGAG
ACTTTTCCACCTTATGAGACCCTTCTTGCCACCCACAGGGAGGGGCTTGAAGTT
Restriction Sites NotI-NotI     
ACCN AK027854
Insert Size 3410 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq AK027854.1, BAB55412.1
RefSeq Size 2923 bp
RefSeq ORF 1515 bp
Locus ID 55054
Domains WD40
Gene Summary The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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