ATG16L1 (AK027854) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	APG16L; ATG16A; ATG16L; IBD10; WDR30
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC102544 sequence for AK027854 edited (data generated by NextGen Sequencing) ATGGCCCAACTGAGGATTAAGCACCAAGAGGAACTGACTGAATTACACAAGAAACGTGGG GAGTTAGCTCAACTGGTGATTGACCTGAATAACCAAATGCAGCGGAAGGACAGGGAGATG CAGATGAATGAAGCAAAAATTGCAGAATGTTTGCAGACTATCTCTGACCTGGAGACGGAG TGCCTAGACCTGCGCACTAAGCTTTGTGACCTTGAAAGAGCCAACCAGACCCTGAAGGAT GAATATGATGCCCTGCAGATCACTTTTACTGCCTTGGAGGGAAAACTGAGGAAAACTACG GAAGAGAACCAGGAGCTGGTCACCAGATGGATGGCTGAGAAAGCCCAGGAAGCCAATCGG CTTAATGCAGAGAATGAAAAAGACTCCAGGAGGCGGCAAGCCCGGCTGCAGAAAGAGCTT GCAGAAGCAGCAAAGGAACCTCTACCAGTCGAACAGGATGATGACATTGAGGTCATTGTG GATGAAACTTCTGATCACACAGAAGAGACCTCTCCTGTGCGAGCCATCAGCAGAGCAGCC ACGAGACGCTCTGTCTCTTCCTTCCCAGTCCCCCAGGACAATGTGGATACTCATCCTGGT TCTGGTAAAGAAGTGAGGGTACCAGCTACTGCCTTGTGTGTCTTCGATGCACATGATGGG GAAGTCAACNNNNNNNNGTTCAGTCCAGGTTCCCGGTTACTGGCCACTGGAGGCATGGAC CGCAGGGTTAAGCTTTGGGAAGTATTTGGAGAAAAATGTGAGTTCAAGGGTTCCCTATCT GGCAGTAATGCAGGAATTACAAGCATTGAATTTGATAGTGCTGGATCTTACCTCTTAGCA GCTTCAAATGATTTTGCAAGCCGAATCTGGACTGTGGATGATTATCGATTACGGCACACA CTCACGGGACACAGTGGGAAAGTGCTGTCTGCTAAGTTCCTGCTGGACAATGCGCGGATT GTCTCAGGAAGTCACGACCGGACTCTCAAACTCTGGGATCTACGCAGCAAAGTCTGCATA AAGACAGTGTTTGCAGGATCCAGTTGCAATGATATTGTCTGCACAGAGCAATGTGTAATG AGTGGACATTTTGACAAGAAAATTCGTTTCTGGGACATTCGATCAGAGAGCATAGTTCGA GAGATGGAGCTGTTGGGAAAGATTACTGCCCTGGACTTAAACCCAGAAAGGACTGAGCTC CTGAGCTGCTCCCGTGATGACTTGCTAAAAGTTATTGATCTCCGAACAAATGCTATCAAG CAGACANNNNNNNNNNCTGGGTTCAAGTGCGGCTCTGACTGGACCAGAGTTGTCTTCAGC CCTGATGGCAGTTACGTGGCGGCAGGCTCTGCTGAGGGCTCTCTGTATATCTGGAGTGTG CTCACAGGGAAAGTGGAAAAGGTTCTTTCAAAGCAGCACAGCTCATCCATCAATGCGGTG GCGTGGTCGCCCTCTGGCTCGCACGTTGTCAGTGTGGACAAAGGATGCAAAGCTGTGCTG TGGGCACAGTACTGA Clone variation with respect to AK027854.1 200 g=>a;670 g=>n;671 c=>n;672 t=>n;673 g=>n;674 c=>n;675 g=>n;676 c=>n;677 a=>n;1267 t=>n;1268 t=>n;1269 c=>n;1270 a=>n;1271 g=>n;1272 t=>n;1273 g=>n;1274 c=>n;1275 a=>n;1276 a=>n 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK027854 unedited GGAATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCCGGCATGAGC CGGAAGACCGTCCCGGATGGCCTCGGGGACTGCCAGTGTGTGGAGGTGAGCTCCGGGATT GCCGGCATTCCCGCTTCTGCTGGTTGCTTCATGCTGCAGGCTGCGGCCGTCAGCCCTCGC TCGCATTGGTGGCGCTGAGGTGCCGGGGCAGCAAGTGACATGTCGTCGGGCCTCCGCGCC GCTGACTTCCCCCGCTGGAAGCGCCACATCTCGGAGCAACTGAGGCGCCGGGACCGGCTG CAGAGACAGGCGTTCGAGGAGATCATCCTGCAGTATAACAAATTGCTGGAAAAGTCAGAT CTTCATTCAGTGTTGGCCCAGAAACTACAGGCTGAAAAGCATGACGTACCAAACAGGCAC GAGATAAGTCCCGGACATGATGGCACATGGAATGACAATCAGCTACAAGAAATGGCCCAA CTGAGGATTAAGCACCAAGAGGAACTGACTGAATTACACAAGAAACGTGGGGAGTTAGCT CAACTGGTGATTGACCTGAATAACCAAATGCAGCGGAAGGACAGGGAGATGCAGATGAAT GAAGCAAAAATTGCAGAATGTTTGCAGACTATCTCTGACCTGGAGACGGAGTGCCTAGAC CTGCGCACTAAGCTTTGTGACCTTGAAAGAGCCAACCAGACCCTGANAGATGAATATGAT GCCCTGCAGATCACTTTTACTGCCTTGGGAGGAAAACTGAGGAAAACTACGGAAGAGAAA CCAGGAGCTGTCACCAGATGGATGGCTGAGAAAGCCCAGGAAGCCAATCGGCTTAATGCA GAGAATGAAAAAGAACTCCAGGAGCNGCAAGCCCCGCTGCAGAAAGAGCTTGCAGAAGCA GCAAAGAACCCTCTACAGTCGACAGGATGATGACATTTGAGGCATTGTGGATGAACTNCT GATACACAGAAGAGACTCTNCTGTGCGAGCATCAGCAGACAGCCCGAGACCN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK027854 unedited NTTTTNAGGGTATTAGCTAGNNACCGCGGCCGCAATCTAGNGATCGATTTTTTTTTTTTT TTTTTGAAAAGTTATAACAGATTTCTTTATTATTATTTACAATCAAGTTCTGTTGGCCAA CATAATGAAATAAATAAAAGATGTGCCCTGGCCTGTGAATTTCAACTCTCCTTGACTTAA GTTCTCTGAAGGGCAAATTGGAAAGCGGTGATCAGGCAGGGAAGAGAGGGCAGGTGGAGG CCAGGACCATCGGTGGGAAGGCCACCTGACTCCTCTCTCACCAGCTCTAACACTCACATC CCCAAATGTCCAGAGAACAAGCATGGAAGAAAAAAAATAAAGTGCAAATTTAAAAGTGAT AAAAAGGGTGTTTCGCACACCCAATGAACTAAAACTTTATACGTAGGTAAAATAGTAAAG ATAAATGTTTTTCCTTGGCCTTCATCACAACCCCTGAAACGGAAAGATGGCGCTGCTGTG CTTCTGAGCCTAGGCTTCTTGCACTAAAGCACCAAGGGCATCGCACACAGGCTTGGCAGA GGGGCCATGGCCAGAATCACCACCTTCAGACAAGTATGNTTGGAGGTCTCGAATCCCTTG GCACCCCCAAGCATGCAGTGTGCACCCACCTTCTGGGTGTCTGCAGATCGATACAGGAAC ACATCCTTACCTCTGCAGCGAACCCATTTTCCTGGAAAAGATTTTCACACATCCTCTCCC ACCTCAAAAAAACAAAAAAACAAAAACCATTTTTTTAAGCCACACGGAGATTTTTAATAT GTCCAAATTTGTGGGGGAAAGAGAGCCCGAAAGCTCTGAAGAACGTGAAAGAAATTTGAG ACTTTTCCACCTTATGAGACCCTTCTTGCCACCCACAGGGAGGGGCTTGAAGTT
Restriction Sites	NotI-NotI
ACCN	AK027854
Insert Size	3410 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK027854.1, BAB55412.1
RefSeq Size	2923 bp
RefSeq ORF	1515 bp
Locus ID	55054
Domains	WD40
Gene Summary	The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2010]

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