FLJ37035 (AK094354) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC102524 sequence for AK094354 edited (data generated by NextGen Sequencing) ATGTTTACCCCGAGTGAAAGTCCCATTCAAAGCCTTGCTTCCTGGAGAGCTTTGAGATTC TGGTACAAAAGGCATTTTATAAGCACAAAGTGGAAAAGCATCATTCATATTCATTTGCTG CTGCGGCATTTCTCCCTGGAATCTTCCTCTTGTCTGTCAACATTGGGCAAAAGGACTGGC CTCATCTACATTACAGATATCCTTGTGATGGTCTGGAGGGACCTGTGGGAGAGGCGGCCA TCCAGAGGGCTCTCTGAGGGGTCATTTAGAATGCAACTCTTCTGGGCAGAGGATGGTCTG CACTGGCCTCCACCACGCAGGAAGGCTTCCAGAGTGGAGAAATGTCCACTAACATGGCTT CTGTGA Clone variation with respect to AK094354.1 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK094354 unedited ATTCGGCACGAGTGACGCTCAGGCTCCGCCCCCTGCATGTCTTTGTTGGCAAACAGTTCT GGGGGTCGTCCGATCCTGCAGAGACCGTCCAGGCTGGACTTGAACTCCTTCCTCGGCTGA AGTGATCCTCCTGCCTCAGCGTCCTGAATAGGAAGTGGAGCCAGAATCCACAGATGTCAT TATCCATCTGCACACTTTGTTTACTACCCAGAAGGCAAGAATATTGCATTTTAAAAGTGC CCGGACTTTTGTTCTTTCTTATCACAAATGACTTGGGAAGCTCCGACCACTCATGTTTAC CCCGAGTGAAAGTCCCATTCAAAGCCTTGCTTCCTGGAGAGCTTTGAGATTCTGGTACAA AAGGCATTTTATAAGCACAAAGTGGAAAAGCATCATTCATATTCATTTGCTGCTGCGGCA TTTCTCCCTGGAATCTTCCTCTTGTCTGTCAACATTGGGCAAAAGGACTGGCCTCATCTA CATTACAGATATCCTTGTGATGGTCTGGAGGGACCTGTGGGAGAGGCGGCCATCCAGAGG GCTCTCTGAGGGGTCATTTAGAATGCAACTCTTCTGGGCAGAGGATGGTCTGCACTGGCC TNCACCACGCAGGGAGGGCTTCCAGAGTGGAGAAATGTCCACTAACATGGCTCCTGGGAG GTATCATTCTGATCATTGGTGGTACAGGAGAGATGAAAGGAAGTTAAGGGGCTGAAAAAC CAGAAACCTGTGGAAATCATGAATCAAAGAAAGGGCGTTTGCTTTTGGCTTCATTTTTT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK094354 unedited NNCGGTATGACTAGNACCGCGCCGCAANCTANGATCGATTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTGACCTTGGACCCCTTTTATTATAGAATTGAAGCCCAGA AGCCAACGACCCCCCTTTGTATTCATGATTTTCAACAGGGCTCCTGGTTCTTCTAGCCCC TCTAACTTCCTCTCATCTCTCCTGGAACAACAATGATCAGAATGATACCCCACAAAAGCC CTGTTAGTGGACATTTCTCCACTCTGGAAGCCTTCCTGCGTGGGGGAGGCCAATGCAAAC CATCCTCTGCCCAAAAAAATTGCCTTCTAAATGACCCCTCAAAAAGCCCTCTGGATGGCC GCCTCTCCCACAGGTCCCTCCAGACCATCACAAGGGTATCTGTAATGTAAATGAAGCCCG TCCTTTTGCCCAATGTTGACCGACAAGAGGAAAATTCCCGGGGGAAATGCCCCACCCCCC AATGAATATGAATGAAGCCTTTCCACTTTGTGCTTATAAAATGCCTTTTGTACCAAAATC TCAAAGCTCTCCAGGAAACAAGGCTTTGAATGGGACTTTCACTCGGGGTAAACATGAGTG GTCGGAGCTTCCCAAGTCATTTGTGATAAAAAAGAACAAAAGTCCCGGCACTTTTAAAAT GCAATATTCTTGCCTTCTGGGTAGTAAACAAAGTGTGCCGATGGATAATGACATCTGTGG ATTCTGGCTCCACTTCCTATTCAGGACGCCTGAGCCGGGGGATCACTTCACCCGAGGAAA GAGTTCAAGTCCAGCCTGGACGGGTCTGCAAGATCGGACGACCCCCAAAACTGTTTGCCA ACAAAACTGCAGGGGGCGGAGCCCGGGCGTCACTGTGGCGAATTCCGGCCGCCTATAGTG GGTCTTA
Restriction Sites	NotI-NotI
ACCN	AK094354
Insert Size	1000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK094354.1, BAC04337.1
RefSeq Size	2554 bp
RefSeq ORF	366 bp
Locus ID	399821

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