MANEA (AY048775) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ENDO; hEndo
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AY048775, the custom clone sequence may differ by one or more nucleotides ATGGCAAAGTTTCGGAGAAGGACTTGCATCATTTTGGCACTTTTTATTCTATTTATTTTCTCTCTGATGA TGGGTTTAAAAATGCTGAGACCAAATACAGCTACTTTTGGAGCTCCTTTTGGACTTGACCTTCTTCCAGA ACTTCATCAACGAACTATTCATTTGGGGAAAAATTTTGATTTCCAAAAGAGTGACAGAATCAACAGTGAA ACAAATACCAAGAATTTAAAAAGTGTTGAAATCACTATGAAACCTTCCAAAGCCTCTGAACTTAACTTGG ATGAACTACCACCTCTGAACAATTATCTACATGTATTTTATTACAGTTGGTATGGAAATCCACAATTTGA TGGTAAATATATACATTGGAATCATCCAGTGTTAGAGCATTGGGACCCTAGAATAGCCAAGAATTATCCA CAAGGGAGACACAACCCTCCAGATGACATTGGCTCCAGCTTTTATCCTGAATTGGGAAGTTACAGTTCTC GGGATCCTTCTGTCATAGAAACTCACATGAGACAAATGCGCTCAGCTTCAATTGGTAATTATTGTATATA TATATATATATGTGTGTTTGTGTCTGTATATATGCATATAAATGATTTTTTGTGTAACTTTACTAGTTAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AY048775 unedited GTCAGCAATTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGCCTGAAACGCC CGGAACGCTGTGCGCGCGCCTTCGGGGAGGAGGGGCTGTCTGGGCTCGGGGCGCGGCGGC AGTCAGCTCTATGTTCGCGGTCTTAACCTCTCCTCTGGCCGAGTCCTTGCAAGAAGTGAA TTACCCGACCCTGCAAAACACTTACTATTTTGGAGTTTAAAGTATGTCATCATGGCAAAG TTTCGGAGAAGGACTTGCATCATTTTGGCACTTTTTATTCTATTTATTTTCTCTCTGATG ATGGGTTTAAAAATGCTGAGACCAAATACAGCTACTTTTGGAGCTCCTTTTGGACTTGAC CTTCTTCCAGAACTTCATCAACGAACTATTCATTTGGGGAAAAATTTTGATTTCCAAAAG AGTGACAGAATCAACAGTGAAACAAATACCAAGAATTTAAAAAGTGTTGAAATCACTATG AAACCTTCCAAAGCCTCTGAACTTAACTTGGATGAACTACCACCTCTGAACAATTATCTA CATGTATTTTATTACAGTTGGTATGGAAATCCACAATTTGATGGTAAATATATACATTGG AATCATCCAGTGTTAGAGCATTGGGACCCTAGAATAGCCAAGAATTATCCACAAGGGAGA CACAACCCTCCAGATGACATTGGCTCCAGCTTTTATCCTGAATTGNGAAGTTACAGTTCT CGGGATCCTTCTGTCATAGAAACTCACATGAGACAAATGCGCTCAGCTTCAATTGGTGTA CTAGCCCTCTCTTGGTACCCACCTGATGTAAATGATGAAAATGGAGAACCTACTGATNAC TGGNTACCCACTANTTTGGATAAAGCTCATAAATATAAACCTANAGGNNNTACTTTCACA TAGAACCCATATAGCATCGAGATGATCAAAACATGTACANAANATGTCAGTATATTATAG ACAAA
Restriction Sites	NotI-NotI
ACCN	AY048775
Insert Size	4530 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AY048775.1, AAL07307.1
RefSeq Size	1096 bp
RefSeq ORF	630 bp
Locus ID	79694
Protein Families	Transmembrane
Gene Summary	N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 [PubMed 15677381]).[supplied by OMIM, Sep 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.