H10 (H1F0) (AK091372) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	H1FV; H10
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK091372, the custom clone sequence may differ by one or more nucleotides AGACGCGGAGCTGGGAAAAGGGAGGCAGAGGAGGCGGAGGCAGAGGCAGAGGCAGAGCCCGGTGCCGAGA CCAAGCGACAGACCGGCGGGGCTGGGCCTCGCAAAGCCGGCTCGGCGAGCTCTCCCGACACCCGAGCCGG GGAGGAAAAGCAGCGACTCCTCGCTCGCATCCCCGGGAGCCGCACTCCAGACTGGCCCGGTAGTCAGGGG CTCAGGAGCAGATCCCGAGGCAGGCTTTGCTCAGCCTCCGACGAGGGCTGGCCCTTTGGAAGGCGCCTTC AACAGCCGGACCAGACAGGCCACCCCAAGTATTCAGACATGATCGTGGCTGCCATCCAGGCCGAGAAGAA CCGCGCTGGCTCCTCGCGCCAGTCCATTCAGAAGTATATCAAGAGCCACTACAAGGTGGGTGAGAACGCT GACTCGCAGATCAAGTTGTCCATCAAGCGCCTGGTCACCACCGGTGTCCTCAAGCAGACCAAAGGGGTGG GGGCCTCGGGGTCCTTCCGGCTAGCCAAGAGCGACGGACCCAAGAAGTCAGTGGCCTTCAAGAAGACCAA GAAGGAAATCAAGAAGGTAGCCACGCCAAAGAAGGCATCCAAGCCCAAGAAGGCTGCCTCCAAAGCCCCA ACCAAGAAACCCAAAGCCACCCCGGTCAAGAAGGCCAAGAAGAAGCTGGCTGCCACGCCCAAGAAAGCCA AAAAAACCCAAGACTGTCAAAGCCAAGCCGGTCAAGGCATCCAAGCCCAAAAAGGCCAAACCAGTGAAAC CCAAAGCAAAGTCCAGTGCCAAGAGGGCCGGCAAGAAGAAGTGACAATGAAGTCTTTTCTTGCGGACACT CCCTCCTGTCTCCTATTTTCTGTAAATAATTTTCTCCTTTTTTCTCTCTTGATGCTCACCACCACCTTTT GCCCCCTTCTGTTCTGACTTTATAAGAGACAGGATTTGGATTCTTCAGAAATTACAGAATAATTCACTTT TCCTTAACCAGTTGTGCAAGGACAGCAACAACCAATCTAATGATGAGAATGTACTTATATTTTGTTTTGC TATTAACCTACTTACGGGGTTAGGGATTTGCGGGGGGGCTTGTGTGTTTTGTTGGCTTGTTTGCCATGAA GGTAGATGTGGGTGGGGAGAAGACACAAGGCAGTTTGTTCTGGCTAGATGAGAGGGAACCCAGGAATTGT GAGGTTAGCAGGAATATCTTTAGGGTGAGTGAGTTTTCCTTGAGTTGGGCACCCGTTGTGAGAGTTTCAG AACCTTTGGCCAGCAGGAGAGAGGTGGTAGGGAGCAGCCAGCCGGCAAAGGAAGGAGGTGGAAAAAAACC GCCACCGGGCTGACTTCCACCTCCCAGTGGTGAGCAGTGGGGGCCCAAACCCAGTTTCCTTCTCATTTTT GTTAGTTTGCCCTTTCGGCCTCCCTATTTTCTTAGGGAAGGGGAGTGGGGTCCAAGTGACAGCTGGATGG GAGAAGCCATAGTTTCTCCCAGTCAGCTAGGATGTAGCCATTGGGGGATCTTTGTGGCTTCAGCAAATTC TCTTGTTAAACCGGAGTGAAAACTTCAGGGGAAGGGTGGGGAGTCAGCCAAGTGCCTCAGTGTGCCCTGT TGAAACTTAGGTTTTTCCACGCAATCGATGGATTGTGTCCTAGGAAGACTTTTCTTTTCCTCTGGATTTT TGTTCCTCCTGTACAAGAGGTGTCTTTGCTTGGTTTGGTGGGGCTGCGGCCACTTAAAACCTCCCGATCT CTTTTTGAGTCCTTTATTATAAGTAGTTGTAGCTGCGGGAGGGGGAGGGGGAGTGGGCGGGCAGTGGATA GTAAGACTTACTGCAGTCGATTTGGGATTTGCTAAGTAGTTTTACAGAGCTAGATCTGTGTGCATGTGTG TGTTTGTGTATATATACATATCTAGGGCTAGTACTTAGTTTCACACCCGGGAGCTGGGAGAAAAAACCTG TACAGTTGTCTTTCTCTTATTTTTAATAAAATAGAAAAATCGCGCACTTGCGCGTCCCCCCCCCACCCCC TTTTTTAAACAAGTGTTACTTGTGCCGGGAAAATTTTGCTGTCTTTGTAATTTTAAAACTTTAAAATAAA TTGGAAAAGGGAGAAACTG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK091372 unedited ACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGGAAAAGGAGGCAGAGGAGGC GGAGGCAGAGGCAGAGGCAGAGCCCGGTGCCGAGACCAAGCGACAGACCGGCGGGGCTGG GCCTCGCAAAGCCGGCTCGGCGAGCTCTCCCGACACCCGAGCCGGGGAGGAAAAGCAGCG ACTCCTCGCTCGCATCCCCGGGAGCCGCACTCCAGACTGGCCCGGTAGTCAGGGGCTCAG GAGCAGATCCCGAGGCAGGCTTTGCTCAGCCTCCGACGAGGGCTGGCCCTTTGGAAGGCG CCTTCAACAGCCGGACCAGACAGGCCACCATGACCGAGAATTCCACGTCCGCCCCTGCGG CCAAGCCCAAGCGGGCCAAGGCCTCCAAGAAGTCCACAGACCACCCCAAGTATTCAGACA TGATCGTGGCTGCCATCCAGGCCGAGAAGAACCGCGCTGGCTCCTCGCGCCAGTCCATTC AGAAGTATATCAAGAGCCACTACAAGGTGGGTGAGAACGCTGACTCGCAGATCAAGTTGT CCATCAAGCGCCTGGTCACCACCGGTGTCCTCAAGCAGACCAAAGGGGTGGGGGCCCTCG GGTCCTTCCGGCTAGCCAAGAGCGACGAACCCAAGAGTCAGGGGCCTTCAAGAAGACCAA GAAGGAAATCAGGAAGGTGCCACCCCAAAGAGGCATCCAGCCCCAGAAGGCCTGCTCCAA GCCCCCACCAAGAAACCCAAGCCACCCCGGTCAAGAGGCCCAGAAGAGCCGGGCTGCCCG CCCCAGAAGCCCAAAAACCAGGACTGTAAAGCCCAAGCGGTCAGAGCATCCAAGCCCAAA AGGGCAAACCATGAAACCCAAGCCAATTCCATGCCCAAGAAGGCCGGCAAGAAAAGGGGA CACAGGAATTTTTTTTGCCGAACACCCCTCCTGGGCCCTATTTCGGAAG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK091372 unedited AATCACTTGNACCGCGCCCGCTTTCTATGATCGATTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCAGTTTCCCCCTTTTCCAATTTAT TTTAAAGTTTTAAAATTCCAAGAACCGCAAATTTTTCCCGGCCCAAGTAACACTTGTTTA AAAAAGGGGGTGGGGGGGGGACCCCCAAGGCCCCTTTTTTTCTATTTTATTAAAAATAAA AAAAAGACAACTGTCCAGGTTTTTTCCCCCACCCCCCGGGGGGGAAACTAATTCCTACCC CTAGATTTGTTTTTTTCCCCAAACCCCCCCTTGCCCCCAGATTTAGCTCTGTAAAACTAC TTAACAAATCCCAAATCGACTGCGGTAAGTTTTACTATCCACTGCCCGCCCATTCCCCCT CCCCCTCCCGGAGTTACAGTTACTTTTAATAAAGGACTCAAAAAAAAATCGGGAGGTTTT AAGTGGCCGCACCCCCCCCAAACCAAGCAAAGACCCCTCTTGTCCAGGAGGAACAAAAAT CCAAAGGAAAAAAAAAGTCTTCCTAGGACACAACCCATCGATTGCGTGGAAAAACCTAAT TTTCAACAGGGCACACTGGGGCACTTGGCTGACTCCCCCCCCTTCCCCTGAAGTTTTCCC TCCGGTTAAACAAAAAAATTTGCTGAACCCCCAAAGATCCCCCCATGGCCTCCATCCCAA CCTGACTGGGGAAAAACTATGGCTTTCTCCCCTCCCCGCTGGTCCCTGTGACCCCCCCCT CCCCCTTCCCTAAAAAAAAAAGGAGGGGCCCAAGGCGCACACTTCCAAATATGAAAGGAA ATCTGGGTTTGGGCCCCCCCTGCTCCCCCTGGGAAGGGGAAACCCCCCCCGGTGGCGTTT TTTTCCCCCCCCTCCCTTTCCCGGTGGGTGTGTCCCTCCCCCT
Restriction Sites	NotI-NotI
ACCN	AK091372
Insert Size	2250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK091372.1
RefSeq Size	2119 bp
RefSeq ORF	2119 bp
Locus ID	3005
Domains	linker_histone
Protein Families	Druggable Genome
Gene Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family. [provided by RefSeq, Oct 2015]

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