FRG1 (AK057099) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC102079 sequence for AK057099 edited (data generated by NextGen Sequencing) ATGGATGAGGGAACCTATATACATGCACTCGATAATGGTCTTTTTACCCTGGGAGCTCCA CACAAAGAAGTTGATGAGGGCCCTAGTCCTCCAGAGCAGTTTTCGGCTGTCAAATTATCT GATTCCAGAATCACCCTGAAATCTGGCTATGGAAAATATCTTGGTATAAATTCAGATGAA CTTGTTGTTGGGCGTTCAGATGCAATTGGACCAAGAGAACAATGGGAACCAGTCTTTAAA ATTGGGAAAATGGCTTTGTCGACCTCAAATAGCTGCTTTATTAGATACAGTGAAGCAGAG GACATAGAAGCAAAAAGTAAAACAGCAGGAGAAGAAGAAATGATCAAGAGTCTCAGTCAT AGCCTGCCTTCATCTCTGAAGGGACCAGGCGACCCACGGAGTGCCTCGGCAGCCTTGTTA TTGCATGCAATCTTCACAGACAAGATCTCACTGCAACCAGGTTTCTACAGGAGCACAGGT GTGACATTATAG Clone variation with respect to AK057099.1 371 t=>c 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK057099 unedited GGGTTCACAAATGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCTCCGCGC AGAAGTCTCCCGGAGCCGACTTCTGAGAGTCGAGTACTCCTATGTTAAGACTACCAAGCT CGTGTTCAAGGGAACCAAGGCGAAGAGGCGAGATGGAAAGAAAGTATCAAGGAGTGAGCT GAAGCCACTGCCCTTGAGAACCCTCTCGAGGAGTCTGGGCTCATGAGGATGCCAGAATAA GTGGCAGTAAGAAGAAAAAGAACAAAGATAAGAAAAGAAAAAGAGAAGAAGATGAAGAAA CCCAGCTTGATATGTTGGAATCTGGTGGACAGTAACAAACTTTGGTGAAAATTTCAGGAA CCATAGCCATTGAAATGGATGAGGGAACCTATATACATGCACTCGATAATGGTCTTTTTA CCCTGGGAGCTCCACACAAAGAAGTTGATGAGGGCCCTAGTCCTCCAGAGCAGTTTTCGG CTGTCAAATTATCTGATTCCAGAATCACCCTGAAATCTGGCTATGGAAAATATCTTGGTA TAAATTCAGATGAACTTGTTGTTGGGCGTTCAGATGCAATTGGACCAAGAGAACAATGGG AACCAGTCTTTAAAATTGGGAAAATGGCTTTGTCGACCTCAAATAGCTGCTTTATTAGAT ACAGTGAAGCAGAGGACATAGAAGCCAAAAGTAAAACAGCAGGAGAAGAAGAAATGATCA AGAGTCTCAGTCATAGCCTGCCTTCATCTCTGAAGGGACCANGCGACCCACGGAGTGCCT TCGCAGCCTTGTTATTGCATGCAATCTTCACAGACAAGATCTCACTGCAACCCAGTTTCT ACAGGAGCACAGGTGTGACATTATAGTGATGACATCATTCTCAGAGAAAATCATTTTACG CACTAAGGACCACAGNTTCAAGGNAGCTTACACAAAGGGATGAC
Restriction Sites	NotI-NotI
ACCN	AK057099
Insert Size	3300 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK057099.1, BAB71366.1
RefSeq Size	2306 bp
RefSeq ORF	492 bp
Locus ID	2483
Gene Summary	This gene maps to a location 100 kb centromeric of the repeat units on chromosome 4q35 which are deleted in facioscapulohumeral muscular dystrophy (FSHD). It is evolutionarily conserved and has related sequences on multiple human chromosomes but DNA sequence analysis did not reveal any homology to known genes. In vivo studies demonstrate the encoded protein is localized to the nucleolus. [provided by RefSeq, Jul 2008]

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