Retinoblastoma binding protein 6 (RBBP6) (BC029352) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DKFZp686P0638; DKFZp761B2423; MY038; P2P-R; PACT; proliferation potential-related protein; RB-binding Q-protein 1; RBQ-1; retinoblastoma-binding protein 6; retinoblastoma-binding protein 6, isoform 3; retinoblastoma binding protein 6; SNAMA
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC029352, the custom clone sequence may differ by one or more nucleotides ATGTCCTGTGTGCATTATAAATTTTCCTCTAAACTCAACTATGATACCGTCACCTTTGATGGGCTCCACA TCTCCCTCTGCGACTTAAAGAAGCAGATTATGGGGAGAGAGAAGCTGAAAGCTGCCGACTGCGACCTGCA GATCACCAATGCGCAGACGAAAGAAGAATATACTGATGATAATGCTCTGATTCCTAAGAATTCTTCTGTA ATTGTTAGAAGAATTCCTATTGGAGGTGTTAAATCTACAAGCAAGACATATGTTATAAGTCGAACTGAAC CAGCGATGGCAACTACAAAAGCAGTATGTAAAAACACAATCTCACACTTTTTCTACACATTGCTTTTACC TTTATAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC029352 unedited GTTCACAATAGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGTAGACCCGC CGGGAGGTGCGGCTCGCGCTATGGACCCCTGACCCCGTGGGGTCGCTCGGACTCTTAACG TGTGGACTGACCGCTACTGACTGCACCGCCAATCCCCCCGTCTCTGCCGGCCCCTTAGCA TGAGCGAGGGGGACCCAGCCGGGTGACATTGTGCCCGTTGGCGGATTCTCGATTTCCCCT CTTCCCCGTCCTCGTCCTCCTCCTCCCCCATGAAGTGATTCTGAGTATCGGGGGGTCTCT GGATTATTGTTCTGACGAACCCCTGCTTGTGGTTGGGGGGTATTTAATCTGAGGCCTTAG GGTCCTTCGGTGTCTTTGAGTGTTTTGTGTGTACATATTTTGCTCTTAAAGTTTATAAAT ATACGTATATTGAGAGTGTCCACGTCTCCTCGCTGAACCTTAGGAATCCCTTGGCACCAT GTCCTGTGTGCATTATAAATTTTCCTCTAAACTCAACTATGATACCGACACCTTTGATGG GCTCCACATCTCCCTCTGCGACTTAAAGAACCAAATTATGGGGAGAGAGAAGCTGAAAGC TGCCGACTGCGACCTGCAGATCACCAATGCGCAGACGAAAGAAGAATATACTGATGATAA TGCTCTGATTCCTAAGAATTCTTCTGTAATTGCTAGAAGAATTCCTATTGGACGTGTTAA ATCTACAAGCAAGACATATGTTATAAGTCGAACTGAACCAGCGATGGCAACTACCAAAGC AGTATGTAAAACACAATCTCACACTGTTTCTACACATTGCTTTTACCTTTATGATGTAGC AGTGAAGTTAATCATTTTAGGACTAATATCCAACTGATCATAGTGCATATTGTACATAAA TGTTTTTTGATGACAGCTCAGNTGATATGGATAATAGTGGCACACCTGCC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC029352 unedited CTTGGACCGCGCCGCTTTCTAGGATCGAGTTTTTTTTTTTTTTTTTTACACTCCAAACAT TTTTTATTGCAAGAAACAAAAAGCACACAACAGCCTGTACATACATACAAAATACTAACT ATAGACAGACAAATGTAGAACATGTCATCATGTTCCTTAGTGTAAAACCTAAACGATCTA AAAAGGATCATACATTAACTGTACAACACAGTGTCATACAGGGATAATGCTATCATATTT AATATGAAACAGTGTTACGGGCACAAATTACCCATTTCTACAAAATAAGTGTGCAAGTGA TGCCACATATTATCCATATTCAACTGAGCTGTCATCAAAATACATTTTATTTACAATATG TACTATGATCAGTTGGATATTAAGTTCTAAAATGATTTACTTCACTGCTACATTATAAAG GTAAAAGCAATGTGTAGAAAAAGTGTGAGATTGTGTTTTTACATACTGCTTTTGTAGTTG CCATCGCTGGTTCAGTTCGACTTATAACATATGTCTTGCTTGTAGATTTAACACCTCCAA TAAGAATTCTTCTAACAATTACAGAAGAATTCTTAGGAAATCAGAGCATTATCTTCAGTA TATTCTTCCTTCGTCTGCGCCTTGGTGATCTGCAGGTCCCAGTCCGCAGCTTTAAACTTT TTTCTCCCCATAATCCGGTTCTCTTAGTTCCCAGAGGGAGATTGTGGAACCCATCAAAGG GGAACGGATCCATAATTAATTTTAGAGGGAAAATTTTTAATGGCCGACCGGACAAGGGGG CCCAAGGGATTTCCCAAGGTTTCACCGAGGGAAAATGGGAACCCTTTTCAATATTACGTT ATTTTATTACACCTTCACAAACCAAAATTTTCCCCCCACCAAAAACCCCGTAACCCCCCG AGGGACCCCTAGGCCCCCTAATTAAAACCCCCCCCACCCCGAGC
Restriction Sites	NotI-NotI
ACCN	BC029352
Insert Size	1200 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC029352.1, AAH29352.1
RefSeq Size	871 bp
RefSeq ORF	357 bp
Locus ID	5930
Protein Families	Druggable Genome
Gene Summary	The retinoblastoma tumor suppressor (pRB) protein binds with many other proteins. In various human cancers, pRB suppresses cellular proliferation and is inactivated. Cell cycle-dependent phosphorylation regulates the activity of pRB. This gene encodes a protein which binds to underphosphorylated but not phosphorylated pRB. Multiple alternatively spliced transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

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