ARSB (AK026942) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ASB; G4S; MPS6
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK026942, the custom clone sequence may differ by one or more nucleotides TTGCATGGAGTAGTCAATTGTGATTGTAGCTTTATTTCATAATATATTAAGACTTGCACTGCTATTTACT AGCAGTGAGAAGAAACCTCAGGAAAGGATATGAAAAAGCAAGTGGCCAGTGTCTGGGATACTGGGCCTTG GTAAAGCAGAGGAGGGCACACCCACAGTCCTCTTATTCTCTGTTTTACTGCTTGTTTTGAGGTTCTGGGG TCTGGCAAAGAGGATGCAGTTTGACACCTGCAGCCCTTTCTCAATCCCACTAATGTCTTACTAATGTGGA ACAGTCCATATTAGCTCCAGAGAGTGTCAAACCCAGAGAAATGTGTGCAAAAATGATACTCTTTTCTGCA TTAGCCCCACCATTGTGTTCACCAATGCTTGGAACACTGCCTGAAGGCACTCATTTTTTAATTTTTATTT TATTTTTAATTTTTTATATCTTTATGAGACGATCTCACTCTGTCACCAGGTTGGAGTACAGTGGTACAAT CACAACTCACCGTAGCCTCAAACTCCTGGGCTCAAGTGATTCTCCCACCTCAGGCACCCAAATAGCTGGA ACTACAGGCATATACCAGCCACACCCAGCTAATTTTATTTTTTGAAAAGACAAGGTTCCCTATGTTGCCC AGCTGGTCTTAAACTCCTGGGCTCCAGCAATTATCCCAGCCTTGGGCCTCCAAAAGTGCATGGGATTACA GGCATGAGTCACCATGCCTGGCCTCATTTTTTAAAACAAATGAATAAATGGACAAATGAGTAAATGAGAA AGTCTCACACCATGAAAGATGCTAGTCCAATGAGCTGAATACAGAGGTAATATAAATGTCTTCCAGCTGT TGCTTTTCTGTTCTCAAGCTGCCCCTCCTGGGGTAGGAGCATAATCTACATCACTGGGCAGTCACAGGAC ACTCTATAGCAAGGTTGTAGCGTCCTCTCCGGTGGGGGGAGAAAAGGAACTGTGCCTACCAAAGGTACTC TCTTGTCAGCAATTTCCATTTCTATACTTTATGGGACACTAGAAACTAAAAGCAACAAATAATCTGATAT AAGTCCTTGTATAGTCATCCTTCAATTCAGTAGCAATATTTTCTGGTCACTACTAACCTGTATTGTATTA AAATGAGACTATTGGAAGGAAATGGTGCTAAAACTAATAACATCTCTTACCAACCTTTACCCAACTCCTG GGTTGGCAAACAGCTGACCAAACTGCCATCACCTCCCACTTGGAAGTGTATGGCCGACAGCATGAAATAG CTGAGCCCAGATGTTCCTTCTGCATCCTCCGAATCCCAGGGCTGGGTGTAGGTAGCCGTTGGAGGCCATC GCTACAGGGCACCTATCTGTTATCGCTGCTGTCCTCCCAACAGCTGTCTCCAGTTCTAGTTCCTTGGTTT TCAGGCACAGTGGGGGATGTTCTGCACCCAGTGGACTTCAAAAGAGTTTTGAAGACTTAATTTTTTGTAA AACAAGTACTTGAGATTTTGGTTTATCCATAATAGAATGTATTTCATTAGATTCTCTGATTCTATATAAG AATGTGAAAAGATTGATATATTGTTGTTAGAAATAATGTTATTTCTTTCCAATTTTTTTTTTTTTTTTTT TTTTGAGATGGAGTCTCGCTCTGTCACCCAGGCCTGGAGTCAGTGGTGTGATCTCGGCTCACTGCAGCCT CTAACTCCCAGGTTCAGGCTATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGATTACAGGCATACACCACC ACGCCTGGCTATGTTTTGTATTTTTCGTGGAGATAGGGTTTCACCATGTTGGCCAGGCTGGTCTCGAACT CCTGACCTCAGGTGATCCACCCACTTCAGCTTCCCAGAGCACTGGGATTACAGGTGTGAGCCACTGTGCC CGGCAATTTTTTTACCTTTACAGAAGGTTTTGCTTATTTAATTGTGAGCTCATTTTTCTTTGTTACTTTT GTCCCCCCAGATTTGGGGGACAAAATAAAATTAATCTTTTAAAATGTGTCAGCCATATGTATGGGGCTTC CATTTGGGGTGAGGAGAAAGTTCTGGAACTAGATAGTGGTCATGGTTATACAACATCATAAATGCATTAC TGCCCTGAATTGTATGTTTTAAAGTGGTTAAAATGTTAAGTTTTATGTTTTATTACATTTTTAAATGTGT CAACCACTTTATAGTACATAAATTATATCTCAGTAAAGCTGTTAAATAAATAAATATAGTAAAAATTTTA GAACTAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK026942 unedited TCAGATTATGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGGCCGACAG CATGAAATAGCTGAGCCCAGATGTTCCTTCTGCATCCTCCGATCCCAGGCTGGGTGTAGG TAGCCGTTGGAGGCCATCGCTACAGGGCACCTATCTGTTATCGCTGCTGTCCTCCCAACA GCTGTCTCCAGTTCTAGTTCCTTGGTTTTCAGGCACAGTGGGGGATGTTCTGCACCCAGT GGACTTCAAAAGAGTTTTGAAGACTTAATTTTTTGTAAAACAAGTACTTGAGATTTTGGT TTATCCATAATAGAATGTATTTCATTAGATTCTCTGATTCTATATAAGAATGTGAAAAGA TTGATATATTGTTGTTAGAAATAATGTTATTTCTTTCCAATTTTTTTTTTTTTTTTTTTT TTGAGATGGAGTCTCGCTCTGTCACCCAGGCTGGAGTGCAGTGGTGTGATCTCGGCTCAC TGCAGCCTCTAACTCCCAGGTTCAAGCTATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGA TTACAGGCATACACCACCACGCCTGGCTATGTTTTGTATTTTTCGTAGAGATAGGGTTTC ACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCAAGTGATCCACCCACTTCAGCTT CCCAAAGCACTGGGATTACAGGTGTGAGCCACTGTGCCCGGCAAATTTTTTTACCTTTAC AGAAGGTTTTGCTTATTTAATTGTGAGCTCATTTTTCTTTGTTACTTTTGTCCCCCCAGA TTTGGGGGACAAAATAAATTAATCTTTTAAAATGTGTCAGCCATATGTATGGGGCTTNCA TTTGGNGTGAATGAGAAAGTCTGGAACTAGATAGTGGNCATGGGTATACCACATCATAAA ATGCANTACTGNCACTGNATNGTATGTTNTAAAAGTGGTAAAAAGGTAAAAGTTTATGTT TATTACAATTTTAAATGTGTCAACCAACTTATAGTACATAN
Restriction Sites	NotI-NotI
ACCN	AK026942
Insert Size	1150 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK026942.1
RefSeq Size	2265 bp
RefSeq ORF	2265 bp
Locus ID	411
Protein Families	Druggable Genome
Protein Pathways	Glycosaminoglycan degradation, Lysosome, Metabolic pathways
Gene Summary	Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targeted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2016]

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