TIGD1 (AK022026) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK022026, the custom clone sequence may differ by one or more nucleotides AGCAATAAAACAAGTCATATGAATGTTTTGGTTTCCCAGTGCATATAAAGTTTTGTTTACACTATACTGT AGTCTATTTGGGTGCAATGGCATTATGTCTAAAAACAATGTAAATACCTTAATTAAAAAATACTATATTG CTAAAAGATGCTACCAATCATCGCTGAGCCTTCAGTGAGTAGTAATCTTTATGCTGGCAGAGGGCCTTGC CTTGATGTTGATAGCTGCTGACTGATCAGAATGTGTTTGGTGAAGCTTGGGCTGGCTGCAGCAATTTTGT AAGACAACAATGAAGTTTGCCTCCTTGATGGATTCTTCCTTTCACGAAAGATTTCTCCATAGCATGCCAT GCTGTTTGATAGGGTTTTACCCACAGTAAATCTTCTTTCAAAATTGGAGTTGATCCTCTCGAATCCTGCT GCTGCCTTATCAACAAAGTTTATAGAATTTTCTAAACCCTTTGTTGTCATTTCAACAAATTTTCACAACA TCTTCGCCAGGAGTAGATTCCAGGTCAAGAAACCACTTTCTTTATTTAACCATAAGAAGCAACTGCTCAT TCATTGAAGTTTTATCATGAGACTGCAGCAAGTCAGGCACATCTTGAGGCTCCACTTATTCTAATTCTCT TGCTGTTGCTACCATATCTGCAGTTACTTCTTCCTCTGAAGTCTTGAACCCCTCAAAGTTATTCATGAGG GTTAAAATCAACTTCTTCCAAACTTCTGTTAATGTTGCTATTTTTACTTCCTCCCATGAATCATACTATT ATTATTATTATTTTTCTTTTTTTTGAGACAGAGTCTTGCTCTGTTTCCCCTGGCTGGAGTGCACTGTCAT GCTTATGGCTTACTGCAGCCTCAACTGGGCCCAAGTGATCCTTGCAAGTAGCTGGGTCTACACCTGAGTT TTTTTTTAAAAACTTTTTATAGAGATGGAGTCTCCCTATATTGCCCAGGCTGGTCTCAAACTTCTGGGCT CAAGGGATCCACCTGCCTTGGCCTCCCAAAGAACTGAGATTACAGACATGAGCCACTGTTCCTGGCCACA AATGTTCCTAATGACATCTAGCATGGTGAATTATTTCCAGAAGGTTTTTAATTTACTTTGCCAAGGTCCA TCAGAAGAATCACTATCCATGGCAGCTATAGCCTTACCAAATATATTTCTTATGAAACTTGAAAGTCAAA ATTACTTATTGATCCATGGGCTGCTGAATGGTGGCTGTGTCAGCAGGCATGAAAACAACATTCATCTCCT TGTACATCCCCATCAGAGTTCTGAGGTAACATTGTCAATGAGGAGTAATATTTCAAAGGATTTTTTTTTC TGGGCAGTAGGTCTCAAGAGTGGACTTAAAATACTCACCAAACCATGCTGAAAACAGATATGCTATCATT TAAGCTTTCTTGTTTCATTTCTAGATGAAGGGCAGTAGGATTTTGGAAATGGTCAGTGACCATTGGCTTC AACTTGAAGTTACCAGTGGCATTAGCTCCTAACAAGGGAGTCAGCCTGCCCTTTGAAGCTTTAAAGCCGG GCATTGACTTTTGCTCTCTAGCTATCAAATCCCTGTATGGCTTCTTCCAACAGAAAGCTGATTTGTATAC ACTGAAAATCCATACACTGTTTAGTGACTTCCTTAATGATCTCAGCTAGATCCTTTGAATATCTTGCTGT AACTTCTGCTTGCGTTTTTATGCTATAGAGATGGCTTTTTTCCCCTTAAACCTCATGAACCAACCTCTGC TAATTTCCCACTTTTCTTTTGCAGCTCCCTCACCTCTCTCAGCTCGCAAAGAATTGAAGAGAGTTAGGGC CTTGCTCTGGGTTAGGCTTTGGCTCAAGAGCATGTTATGGCTGGTTTAATCACTCAAACTTACTCCATAT CAACAGTAAGACTTTTGTTTTCTTACCATTCATGTGTTCACTGGAGGAGCAATTTTAATTTTCTTTGAAA ACTTTTCTTTTGCATTTACAACTTGGCTGTTTGGTGCAAGAGGCCCAGTTTTTGGCCCATTTTGGCTTTC TACATGCCTTCCTCACTAGGCTTAATCATTTCTGGCTTTTGATTTAATGTAAGAGACATGCAACTCTTTC TTTCAAACTTGAACACTTAAAGCCCATTGTAGAGTTATTAATTGGCCTAATTTCAGTAGTATTATGTCTC AGGGAATAGGGAGTCCCCGAGAGAGATGGAAGAATGGCTGGTTGGTGGAACAGTTCACAACATTTATGGA TGCAGTTTGCCATCTGATATGGGCCCCAATATAATTATAATAGTAACATCAAAGATCCCGGTCACAAGTC ACTATAACAAATATAATAATAGTGAAAAAGCTTGAAGTATTTTGAGCATTATCAATATGTGACACAGATA AATGAAGTGTGTACATACTATTGGAAAAATTGTGCCGATAGACTTGCTGGATGCAGGGTTACCACAAACC TTCAATTTGC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK022026 unedited TCAGAATTTTGTAATACGACTCACTATAGGGCGGCCCGCGAATTCGCACGAGGGTCTACA CCTGACTTTTTTTTAAAAAACTTTTTATAGAGATGGAGTCTCCCTATATTGCCCAGGCTG GTCTCAAACTTCTGGGCTCAAGGGATCCACCTGCCTTGGCCTCCCGAAGATCTGAGATTA CAGACATGAGCCACTGTTCCTGGCCACAAATGTTCCTAATGACATCTAGCATGGTGAATT ATTTCCAGAAGGTTTTTAATTTACTTTGCCAAGGTCCATCAGAAGAATCACTATCCATGG CAGCTATAGCCTTACCAAATATATTTCTTATGAAACTTGAAAGTCAAAATTACTTATTGA TCCATGGGCTGCTGAATGGTGGCTGTGTCAGCAGGCAGGAAAACAACATTCATCTCCTTG TACATCCCCATCAGAGTTCTGAGGTAACATTGTCAATGAGGAGTAATATTTCAAAGGATT TTTTTTTCTGGGCAGTAGGTCTCAAGAGTGGACTTAAAATACTCACCAAACCATGCTGAA AACAGATATGCTATCATTTAAGCTTTCTTGTTTCATTTCTAGATGAAGGGCAGTAGGATT TTGGAAATGGTCAGTGACCATTGGCTTCAACTTGAAGTTACCAGTGGCATTAGCTCCTAA CAAGGGAGTCAGCCTGCCCTTTGAAGCTTTAAAGCCGGGCATTGACTNTTGCTCTCTAGC TATCAAATCCCTGTATGGCTTCTTCCAACAGAAAGCTGATTTGTATACACTGAANATCCA TACACTGGNTTAGTGACTTCCTTAATGATCTCAGCTAGATCCTTTGAATATCNTGCTGTA ACTTTCTGCTTACGTTTTATGCTTTAGAAGAGGNNCTTTTTTCCCTTNAAACTCATGAAC CAACCTCTGCTAATTTCCCACTTTTNCTTTTGCAGCTCCTCACTCTCTCAGCTCGCAAGA AAATGAGAAAAGTANGGCCTTGCTTTGGGT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK022026 unedited ATTCGGGGTTTGTGGCTACCTCTGCTNCCANCAAGTCTATCGGCACAATTTTTCCAATAG TATGTACACACTTCATTTATCTGTGTCACATATTGATAATGCTCAAAATACTTCAAGCTT TTTCATTATTATTATATTTGTTATAGTGACTTGTGACCGGGATCTTTGATGTTACTATTA TAATTATATTGGGGCCCATATCAGATGGCAAACTGTATCCATAAATGTTGTGAACTGTTC CACCAACCAGCCATTCTTCCATCTCTCTCTTGGGGACTCCCTATTCCCTGAGACATAATA CTACTGAAATTAGGCCAATTAATAACTCTACAATGGGCTTTAAGTGTTCAAGTTTGAAAG AAAGAGTTGCATGTCTCTTACATTAAATCAAAAGCCAGAAATGATTAAGCCTAGTGAGGA AGGCATGTAGAAAGCCAAAATGGGCCAAAAACTGGGCCTCTTGCACCAAACAGCCAAGTT GTAAATGCAAAAGAAAAGTTTTCAAAGAAAATTAAAAATGCTCCTCCAGTGAACACATGA ATGGTAAGAAAACAAAAGTCTTACTGTTGATATGGAGAAAGTTTGAGTGATTAAACCAGC CATAACATGCTCTTGAGCCAAAGCTTAACCCAGAGCAAGGCCCTAACTCTCTTCAATTCT TTGCGAGCTGAGAGAGGTGAGGGAGCTGCANAAGAAAAGTGGGAAATTAGCAGAGGTTGG TTCATGAGGTTTAAGGGGAAAAAAGCCATCTCTATAGCATAAAAACGTAAGCAGAGTTAC AGGCAGATATTCAAAGATCTAGCTGAGATCATTAAGGAAGTCACTAAACAGTGTATGGAT TTTCAGTGTATACAAATCAGCTTTCTGTTGGAAGAAGCCTACAGGGATTTGATGCTAGAG AGCAAAATTCATGCCCGGCCTTTAAGCCTTCAAGGG
Restriction Sites	NotI-NotI
ACCN	AK022026
Insert Size	1750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK022026.1
RefSeq Size	2460 bp
RefSeq ORF	2460 bp
Locus ID	200765
Protein Families	Transcription Factors
Gene Summary	The protein encoded by this gene belongs to the tigger subfamily of the pogo superfamily of DNA-mediated transposons in humans. These proteins are related to DNA transposons found in fungi and nematodes, and more distantly to the Tc1 and mariner transposases. They are also very similar to the major mammalian centromere protein B. The exact function of this gene is not known. [provided by RefSeq, Jul 2008]

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