KRT86 (AK057905) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK057905, the custom clone sequence may differ by one or more nucleotides CATGCAGCTACAGCTGGAAGGGAAGAAGCACTACCCAGGCAAGCTGACCCCAAGCCTTAATTAGGCTTCT CAGTAACAGGAATACCTGCTGCCAGATTACTGGATCTCAACTGAGATGAACAAGACTGCAATCTCAGATC AGTGCCTGGGTGAACATGAGAGACTCAAACGGGGCTAGATCATACTTAAAGGACTTACACAAATTACCTG TCTGGTTGCAGGGTGGGGAGGTTAAGAAACAAAACTTAAATGCTGCCAGGAGTGTGAGGACAGAGCACAC TGAGAACCCTGGCAGGGCTGGGAAAGGGAAAGGTCTATGGGACCCACCTCTGCTCCTCGCCCTCCAGCAG GCGCCTGTAGGTGGCGATCTCGATGTCCAGGCCCAGCTTGGAGTTCATCACCTCCTGGTACTCCCTGATC AGGCAGGCCATGTCCTGCTTGGCCTTCTGCAGGGCACCCTCCAGCTCGGCCAACTTGCAGCGGGCATCGC TGAGGGCCGCCTCACCCTGCTGCTCAGACTGAGCCACCGCAGCCTCCAGCTTGGAATTCTGAAGAGAACA GAGAGAACAAGGTAGATTAGAGTCCCTGGGTCTCTCTGATGCTTACCTCGATGAGACCATGCTCCCCACC AAGCTGGGAGCACCCCAGAACAGAGCCTCTTGCCTTCATCACCTGGGACTCACAGAGACCACAACCAGGG ACTCTACATGGACAGAGGGGCTGGAGAATGAATGCTGAGATCCAGATAGGGCACACATAGGCTGTGTGAC CATGGTTAGACCCTCGGTCTCTCTGACCTTGGGCACAGATGCCCCATACCTGGCACTTGGCATTCTCCAC CTCAGCCGTCAGCCTCTGGATCATGCGGTTCAGCTCGTTGATCTCCTCCTTGGTGCGGCGCAGGGTCTCC CCGTGCCTGATCACCGTGGCCTTCATCTCCTCACACTGGGGGAAGTAGAGATGCTCATGAGGTTCAGGGT GGGACCTCCCACCCATTCAGGGCATGACAGATGATTTTGCAGGTTGTGCAGTGCTCAGCCTGTGCAACCA CACATGGCAGTCCTGCCCTACCACCCCAACATCAGAGTGGCAGCCATCTCTTCTCATCCCTAGTGCATCC CCAGAAAAGGAAGCCTATTTAATAGATAATCTCAAATCCCTCCCACTGCCATGTCTAGCAGGCAGGTGTC CTGTGCCACTCACCTTGCTGCGGTACCAGGACTCGGCCTCAGCCCGGCTACGGGTGACAATGTCATCGTA CTGTGCCTTGATCTCGGCAATGATGCAGTCCATGTTCAGGTCCCGGCTGTTGTCCAGCTTGACAACCACG GAGGTGTCTGAGATGTGGGACTGGAGAACGCGGATCTCCTGCAGGAGGTGAGGGCAGTGACTTTAGTTGA GAACACAGCCCCACCCACCATGTCCTGACTCCACCTCCTCAGGCTTTCTCTGGTCCCCAACCCTGCCCCC TCACACAGCCTCAGGGACTGCAACCTCTACCCACATCCTGTCCAACACTCCCACCCCCATCTCTCCTCTC TGCTGGCTGCCAGGTCTCTGCCTGGCCCCTGAGCCCGCACCTCCTCATACAGCCGCCTCAGGAAGTCGAT CTCCTGGATCAGGGCCTCCACATTGGCCTCCAGGTCTGATTTGCGGAGGTAGGCGCAGTCCACATCCTGG AAAGGTGGGGAGTGTTGGAGCTCAAGGACCCTGGTGATCCAGCCTCTAGATTCCTCTCTCTTCCCACCCA TTCCATGAAGCCTGGGGAAAGGAGTCCCCAGTGTCTCATTCCCATGCCCTCTCCTTCCCTTCTGGCCCTT CCCGGGGTGACCTGGCATCCTCTGCCATTCTGAGGACAAACCCTGTTTGTCTTGGGCACGGCTCACCAGC CTTTCACTGCTCCTTACCCTCTTATCTCTCCTTTCTCCCCTCTCCTGCTCCAGGAGGCCTGCCCAGACTG ACCCCCCAGCTCTCACACCCTGACCCTTTCCTGTCCCCCAGCAGTGCTCCAAAAGCCCAAGGGCTACTCC TGGTTCTTCTCCATCCCCCCTTAGAACCTGGGATTGTGTCTCTGCCTCAAGGTCAGACTCCCCAAAATGC TAGACAGACTGCACTGTCTCTCAGCAGAGACATTGTATCTGCTATGGCTCCAGGGGCCTCAGCAATAGAC CACAACCTCTGGAACAGAGCAGGACACACAGAGGACAGTGCAGCCATCCCCTTGCCCAAGGTAGGTGCTG GTAGATATTCCCAAGTCATTGCCCTTTGTCAAGGCCCTGGATGCTCAATACAAAGGCCTGCATCTGGGCA GTCCTAGCCTGCTGTGGGAAGGTCATTGTGCCCCACGCTGAGGTTGAGACCCCCTGTGTGTCCCCAGGAA GAATCTGTCATTCTGAGATCCCACAGATCTGTGCTTCTC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK057905 unedited NGTCAAAATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGGCTGGAAGG GAAGAAGCACTACCCAGGCAAGCTGACCCCAAGCCTTAATTAGGCTTCTCAGTAACAGGA ATACCTGCTGCCAGATTACTGGATCTCAACTGAGATGAACAAGACTGCAATCTCAGATCA GTGCCTGGGTGAACATGAGAGACTCAAACGGGGCTAGATCATACTTAAAGGACTTACACA AATTACCTGTCTGGTTGCAGGGTGGGGAGGTTAAGAAACAAAACTTAAATGCTGCCAGGA GTGTGAGGACAGAGCACACTGAGAACCCTGGCAGGGCTGGGAAAGGGAAAGGTCTATGGG ACCCACCTCTGCTCCTCGCCCTCCAGCAGGCGCCTGTAGGTGGCGATCTCGATGTCCAGG CCCAGCTTGGAGTTCATCACCTCCTGGTACTCCCTGATCAGGCAGGCCATGTCCTGCTTG GCCTTCTGCAGGGCACCCTCCAGCTCGGCCAACTTGCAGCGGGCATCGCTGAGGGCCGCC TCACCCTGCTGCTCAGACTGAGCCACCGCAGCCTCCAGCTTGGAATTCTGAAGAGAACAG AGAGAACAAGGTAGATTAGAGTCCCTGGGTCTCTCTGATGCTTACCTCGATGAGACCATG CTCCCCACCAAGCTGGGAGCACCCCAGAACAGAGCCTCTTGCCTTCATCACCTGNGACTC ACAGAGACCACAACCAGGGACTCTACATGGACAGAGGGGCTGGAGAATGATGCTGAGATC AGATGGGCACACATAGCTGGTGACCATGGTAGACNNCTCGTCTTCTGACCTTGGCACAGA TGCCCATACCTGCACTTGGCATTCTCACCTCANCGTCAGCTCTGANNCATGCGTCAGCTC GTGACTCTTCTGGCCGGGGCAGGTCTCCGTGCTGANACCGTGGC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK057905 unedited AATGGGGGTTGGACAGAACCCTTTTCNGGGGTACACACAGGGAGGTCTCAACCTCAGCGT GGGGCACAATGACCTTTCCACAGCAGCTAGGACTGCCCAGATGCAGGCCTTTGTATTGAG CATCCAGGGCCTTGACAAAGGGCAATGACTTGGGAATATCTACCAGCACCTACCTTGGGC AAGGGGATGGCTGCACTGTCCTCTGTGTGTCCTGCTCTGTTTTAGAGGTTGTGGTCTATT GCTGAGGCCCCTGGAGCCATAGCAGATACAATGTCTCTGCTGAGAGACAGTGCAGTCTGT CTAGCATTTTGGGGAGTCTGACCTTGAGGCAGAGACACAATCCCAGGTTCTAAGGGGGGA TGGAGAAGAACCAGGAGTAGCCCTTGGGCTTTTGGAGCACTGCTGGGGGACAGGAAAGGG TCAGGGTGTGAGAGCTGGGGGGTCAGTCTGGGCAGGCTTCCTGGAGCAGGAGAGGGGAGA AAGGAGAGATAAGAGGGTAAGGAGCAGTGAAAGGCTGGTGAGCCGTGCCCAAGACAAACA GGGTTTGTCCTCAGAATGGCAGAGGATGCCAGGTCACCCCGGGAAGGGCCAGAAGGGAAG GAGAGGGCATGGGAATGAGACACTGGGGACTCCTTTCCCCAGGCTTCATGGAATGGGTGG GAAGAGAGAGGAATCTANGAGCTGGATCACCAGGGTCCTTGAGCNNTCACACTCCCCACC TTTCCAGGATGTGGACTGCGCCTACCTCCGCAAATCAGACCTGGAGCCCAATGTGGAGCC CTGATCCAGGAGATCGACTTCCTGNAGCGGCTGTATGAGGAGGTGCCGGCTCAGGGGCCC AGCAGAGACCTGGCANCCANCANANAGAAAGATGGGGTGGGAGTGTTGACAGATTGGTTA NAAGGTTTCAATCCCCTAGACTTTTTAAGGGGCCAGTTTGGGACCCAAAAAGCCTT
Restriction Sites	NotI-NotI
ACCN	AK057905
Insert Size	2500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK057905.1
RefSeq Size	2419 bp
RefSeq ORF	2419 bp
Locus ID	3892
Gene Summary	This gene encodes a type II keratin protein, which heterodimerizes with type I keratins to form hair and nails. This gene is present in a cluster of related genes and pseudogenes on chromosome 12. Mutations in this gene have been observed in patients with the hair disease monilethrix. [provided by RefSeq, Feb 2016]

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