PSMD5 (AK001065) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	S5B
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK001065, the custom clone sequence may differ by one or more nucleotides AAAAACTGATGAAGGAAATTGAAGAAGACACAAATAAATGGAAAGGTATCCTATGTTCATGGATTGGAAG GATTAATATTATTAAAATGTTCATGCTGCCCAAAGCACTATATAGATTCAGTGCATTCCCTCCAAAATTT TTAAATTTAATGACATTTTTCACGAAAGCAGAAAAAATAATTTTTAAATATACATATTTCAGAATACATT GTTCACAATAAAGATATATGATTTTTTGTCAGTTAAAAGTTAATTTTTTTAAAAAGATACCTATAGCCTC TTATTGAGGTGGAATCTGTTGCTCTTGGAGCTACGTACTGTTGACTTTGGTTTTCTGGAAACATCAGTGC TATTTAGAACATTAGGATATTTTTCTTTTTGAATATCAACAAAAACATAAATAAAAATACTAGTAATGAC ATTTACAGTATGTTTCACAGATTAATCACATCTCTGAGTGGGAGTCTTTATCTGTTGTAGGATCTACTCT TGCCATTCTATTCTGGGTTTTGGTTGATTAGTCTTTCTTGAACACTTCCAGAAATTGGAGATGTATGCCT GCCTTAGGTAGCCTAGCTTATTTTATTAATGAACATATCCATTTAAAAATTTCTTCCTGGTAATGAGTCT AAACTTGCCTTCCTGTAATTTGTATCCACTGGTCCTTTTTCTTTGTTCTCCCTCTTGCATATGCCAGTAC TTCAATAGCTGGTCCCAGCTCCATTGACGCCCTCTCTCAGGATCACACATCACAGGTCCTTCAGAATCTC CTAATGGTCTCTCTGATCTCCAGGCAACACACTACTCTAACCTTTCTATGCTTTTCTGGCCTGCAGTTCA TTTCTGTTCCTGTGAAGTCATAGAGCCCGCAATTCATGCTGTCTTGTTCCCCTATTAAAATATATTTTTG TAAATCTCTTTAGGATTCGTGAAGTTTTTTGGAAACCTGGCTGTCATGGATAGTCCTCAACAGATCTGTG AGCGTTATCCTATCTTTGTGGAAAAAGTCTTTGAAATGATAGAAAGTCAGGACCCCACTATGATTGGTGT AGCTGTAGACACAGTTGGAATCTTGGGATCCAATGTTGAAGGAAAACAGGTTTTACAGAAAACAGGTTGG TATGACTTGGCCCTGTTCAGGGATATCTTAATCTTTGACAGGGGTTCAATATGTAAGTTCATTTGCTGGT GGGTTAACTACATTGTAGAAACTATTTTATGGGTGGCAGTCTTGTTAACCATGACCTTGTACTCATGTCT GAAAATCTAATAATTATTACGGAAAACCTTTTTTTTTTTTTTTTGACTTTTCACTGGTTTAGGTTTTCAT TGGTTTAGGTTTCTACCAATCGATTTCAACCAAACATTGTTTCCTGTTTCCCCTCTATGATGTCTTCTTT GACTAGATTTCATGGTACAAAGACCACGGGCTAGAGTCAGAAAGGTCTGGATTCATGTCCTTGGGTTTTC TATTCTTTTTTTTTTTTTTTTTTTAAGAGATTGAGAGTGTCTCTCTGTTACCCAGGCTGGCCCCGAACTC CCGGGCTCAAGCAATCCTCCTGCCTCATCCTCTTGATTGGCTGGGTCTACAGGTCCACACCATTGTATCC AGCCCTGACTTTGCTTTTTGAACTTCATTTACTCACTTCACTTTTCTAGGCCTCATTTTCTTCATTTATA AAAATGGCATAACAACTGCTTCTCAGGGTTGTTGTGAATATTTATGAAATTTTGAGAAAGGAAAATAAAA GTTTGTTATAAGATTTCATGTTAAGAGATACTTTTGGCATGTTAAGAGATACAATGGTTCATGCCTGTAA TCCAGCACTTTGGGAGGCTAAGGTGGGTGGGTTGCCTGAGCTCAGGAGTTCAAGACCAGGCTGGGCAACA TGGCAAAATCCTATCTCTACTCAAGAATACAAAAATTAACCAGGCATGGTACTGCACAACTGTAGTCCCA GCTACTTGAGAAGCTGAGGAAGGAGAATCTCTTGAACCTGGCAGGTGGAGGTTGCAGTGAGCTGAGATCA TGCCACTACACTCCAGCCTGGGTGACAGAGTGAAATTCTATCTCAAAAAAAAAAAAAAAAAAGAGGCCAG GTATGGTGGCTCATGCCTGTAATCCCAGCATTTTGGGAGGCCAAAGCAGGTGGATCACTTGAGGTCAGGA GCTCGAGACCAGCCTGGCCAACATGGTGAAACCCTGTCTCTACTAAAAGTACAAAAATCAGCCAAGCGTG GTGGTGCATGCCTGCAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGCATGAACACAGGAGGTAA AGGTTGCAGTGAGCCGAGATCACACCACTGCACTCCAGCCTGGGCGACAGTGCGAGACTCTGT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK001065 unedited GTCAGAAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGTAGCCTAGC TTATTTTATTAATGAACATATCCATTTAAAAATTTCTTCCTGGTAATGAGTCTAAACTTG CCTTCCTGTAATTTGTATCCACTGGTCCTTTTTCTTTGTTCTCCCTCTTGCATATGCCAG TACTTCAATAGCTGGTCCCAGCTCCATTGACGCCCTCTCTCAGGATCACACATCACAGGT CCTTCAGAATCTCCTAATGGTCTCTCTGATCTCCAGGCAACACACTACTCTAACCTTTCT ATGCTTTTCTGGCCTGCAGTTCATTTCTGTTCCTGTGAAGTCATAGAGCCCGCAATTCAT GCTGTCTTGTTCCCCTATTAAAATATATTTTTGTAAATCTCTTTAGGATTCGTGAAGTTT TTTGGAAACCTGGCTGTCATGGATAGTCCTCAACAGATCTGTGAGCGTTATCCTATCTTT GTGGAAAAAGTCTTTGAAATGATAGAAAGTCAGGACCCCACTATGATTGGTGTAGCTGTA GACACAGTTGGAATCTTGGGATCCAATGTTGAAGGAAAACAGGTTTTACAGAAAACAGGT TGGTATGACTTGGCCCTGTTCAGGGATATCTTCATCTTTGACAGGGGTTCAATATGTAAG TTCATTTGCTGGTGGGTTAACTACATTGTAGAAACTATTTTATGGGTGGCAGTCTTGTTA ACCATGACCTTGTACTCATGTCTGAAAATCTAATAATTATTACGGAAAAACCTTTTTTTT TTTTTTTTGACTTTTCACTGGTTTAGGTTNTCATTGGTTTANGTTTCTACCAATCGATTT CAACCAAACATTGTTTCCTGTTTCCCCTCTATGATGTCTTCTTTNGACTAGATTCATGGN ACAAAGACACNGNCTANAGTCAGAAAAGTCTGGATTCATGTCCTC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK001065 unedited NNAAAATACTGTGNACCGCGGCCCGCTATCTANATCGAGTTTTTTTTTTTTTTTTTTACA GAGTCTCGCACTGTCGCCCAGGCTGAAGTGCAGTGGTGTGATCTCGGCTCACTGCAACCT TTACCTCCTGTGTTCATGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTGCAGG CATGCACCACCACGCTTGGCTGATTTTTGTACTTTTTTTTTTTACAGGGTTTCACCATGT TGGCCAGGCTGGTCTCGAGCTCCTGACCTCAAGTGATCCACCTGCTTTGGCCTCCCAAAA TGCTGGGATTACAGGCATGAGCCACCATACCTGGCCTCTTTTTTTTTTTTTTTTTTTTGA GAAAAAATTTCACTCTGTCACCCAAGCTGGAGTGTAATGGCATGATCTCAGCTCACTGCA ACCTCCACCTGCCAGGTTCAAGAAATTCTCCCTCCTCAGCCTCTCAAGTAGCTGGGACTA CAGTTGTGCAATACCATGCCTGGTTAATTTTTGTATTCTTGAGTAGAGATAGGATTTTGC CATGTTGCCCAGCCTGGTCTTGAACTCCTGAGCTCAGGCAACCCACCCACCTTAGCCTCC CAAAATGCTGGATTACAGGCATGAACCATTGTATCTCTTAAAAGCCCAAAGTATCTCTTA AACATGAAAACTTAAAAAAAATTTTTTTTTTCTTTTCCAAATTTTATAATATTTAACAAA AACCCTGAAAAACCTTTGTATGCCATTTTATAAATTAACAAAAGTAGGCCTCAAAAATTA ATTGGGTTAATGAATTTCAAAACCAAATTCCGGCTGGAATCATGGGGGGGACCCTTTAAC CCCCCCCCCTAACAAGTTAAGCCCGGAGGATTTGTTTAACCCGGGAATTA
Restriction Sites	NotI-NotI
ACCN	AK001065
Insert Size	2250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK001065.1
RefSeq Size	2373 bp
RefSeq ORF	2373 bp
Locus ID	5711
Gene Summary	The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a non-ATPase subunit of the 19S regulator base that functions as a chaperone protein during 26S proteasome assembly. [provided by RefSeq, Jul 2012]

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