SCN8A (AK091315) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CERIII; CIAT; EIEE13; MED; NaCh6; Nav1.6; PN4
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC101355 sequence for AK091315 edited (data generated by NextGen Sequencing) ATGGGCCTTGGTATCTGGCACCCATCAGCCAGCCTCATAGGAAAGAAGCCACCTCCGCTG TATTTGAAGTTTCACTTTTTAAAAAAAAAATGTTTCCCACCTTTTTATCCTTCACCCACT GTCCTTCCACCTGCTCACTCACTCACTCTCTCACCCATCCTGCTCCACACTTCTTTGGTA GTAAATGACACCATCACCAGCAGTTTACACCCGCAGTTAACAGGCATCGAACTGAAAGAA TCAGTGATGACGTTTTTGTGCATCTTCGCTGAGTGGGTAAGTGGCAACGCTTTGCCAAAT ATTAACGAAGCCAATCAAAGAGCAGCGCAGCCACAGTATCACTGCACATATATATATAGA TATATAAATATAATATAA Clone variation with respect to AK091315.1 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK091315 unedited GTCAGAATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGAATTTTTGTA CTACACCCAATACCAGACTGGGAAGGCCTATCTTTCAGTGACCCTGCTAACTGCTAGGAT GAATGTATAGTAACATGTACAGGCTACATTGTAAACAGCACAAAACAGAAGCTGACATGT GTGGTTATTCTTTTTAAGAGAATTATAAATACTGTAATATATAATTTTATTTTATTGGCA TGCCTTTTTGTAAATACAAATTATTAACTTATTAAATAGGAAATGGCTTCTGGCTTATGC CATTGTCATGTTTATTTTTATTTTTTATACTTTTCTTTCTTCTTAGAACTTAGATGCTGT CAGCCAATGTACATCCCCAAACCAGACAGACAGACAAAAAATTTTTTATTGCTAATGGTC TTTTCCAAAACAACAAAAAATATATATTTTTGTTCCAATGTGCAATAAATTCTAACCACG TCTATGCAAGATTTGGCTAAACTTAATAATTGTTCTTAGGTCTTGAGATGGGCAACAGAG CTGTAAGATCCCGCCAAGTACACCATGTGCTCATGCTAGTGATGTCATTAAGCTACTAGG GCATAGTGATGAGATTTTTCTGAGATCTAACCGTTTCCCTCCGCTAGCTCCTGACCCCAG CCCAGCAGGCTGTCTCCTCAGTAACACATGNGGCCTTGGTATCTGGCACCCATCAGCCAG CCTCATAGGAAAGAAGCCACCTNCGCTGTATTTGAAGTTNCACTNTNTAAAAAAAAAATG TTTCCCACCTTTTTATCCTTCACCCACTGTCCTTNCACCTGCTCACTCACTCACTCTCTC ACCCATCCTGCTNCACACTTNTTTGGTAGTNAATGACACCATCACCAGCAGTTTACACCC GCAGTTAACAGGCATCGAACTGAAGAATCAGTGATGACGTTTTTGTGCATCTTC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK091315 unedited NNAAATACTGGTACCGCGCCGCTATCNANNGATCGGTTTTTTTTGTTTTTTTTTGGCTTA GAGGAGTGATGGACTTTTTATCACGTCTCATTAGGGCAGCATTGGCCTCCATGCATTCAC TAATACACTAATCTTCACTGTTGGTCTTTTACAGGTTTACGTTGGTTGCAGCAAAAAATA AAAGAACTCTGGGCACTGTCGCTTTAGGGGCTTTGACTGGGAGTTTGTTTATCTCTCTCC AGTCAACTTCTGACGCCAAAAGTGGCTCGTTTAGAAAGGAGTGAGAGTAGGCCATGTTAA CATGGAAGTTTCTTCCTCCTCTCGTCCTCTGCTTTAAATTACAGAGTCTCCTCATCCTAA GCAACAAGGAGGTTAAGGAAAGAAGAGGGGGAAAATGTTTTTCACACACAAACCAGCAAC CATGTCAGCTGCTGCTCAGTGGCTTTGGCTCTGCATTTTAAATTATTAACAGCATCACAC ATCTTTTTGCTTTTTGTCCTTTTTTTAAAAGGTGGATTGTGGAGTATAAATACTGCACCA AGGACCTGGCTACAAAAAATAAATATTTATATTATATTTATATATCTATATATATATGTG CAGTGATACTGTGGCTGCGCTGCTCTTTGATTGGCTTCGTTAATATTTGGCAAAGCGTTG CCACTTACCCACTCAGCGAAGATGCACANAAACGTCATCANCTGATTCTTTCAGTTCGAT GCCTGTTAACTGCGGGTGTAAACTGCTGGTGATGGTGTCATTTACTACCAAAGAAGTGTG GGACANGATGGGTTGAAAAAGTGAGTGAGTGAACAAGTGGGAAGGACAGTGGGTGAAGGA TAAAAAAGTGGGAAACATTTTTTTTTTAAAAGATGAAACTTCAAATCAGCGGAAGTGGCT TTCTTTCT
Restriction Sites	NotI-NotI
ACCN	AK091315
Insert Size	1750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK091315.1, BAC03635.1
RefSeq Size	2849 bp
RefSeq ORF	378 bp
Locus ID	283401

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