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SPRED1 (NM_152594) Human Untagged Clone
CAT＃: SC101247
SPRED1 (untagged)-Human sprouty-related, EVH1 domain containing 1 (SPRED1)
CNY 3,656.00
CNY 7,220.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | hSpred1; LGSS; NFLS; PPP1R147; spred-1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC101247 sequence for NM_152594 edited (data generated by NextGen Sequencing)
ATGAGCGAGGAGACGGCGACTTCTNNNNNNGATAATAGTTATGCACGAGTGCGAGCTGTG GTGATGACCCGAGATGACTCAAGTGGTGGATGGTTACCACTTGGAGGGAGTGGACTAAGC AGCGTCACTGTCTTCAAAGTCCCTCATCAGGAAGAGAATGGCTGTGCTGACTTTTTTATC CGTGGAGAGCGACTCAGGGACAAAATGGTGGTTTTGGAATGTATGCTTAAAAAAGACCTC ATTTATAATAAGGTCACTCCAACATTTCACCACTGGAAGATTGATGACAAAAAGTTTGGT CTTACGTTTCAAAGTCCTGCTGATGCTAGGGCTTTTGATAGAGGTATCCGAAGAGCTATA GAGGATATTTCTCAAGGATGCCCCGAATCAAAAAATGAAGCTGAAGGGGCAGATGACTTA CAAGCAAATGAAGAGGATTCTTCCAGTTCTCTAGTGAAGGATCACCTTTTTCAGCAAGAG ACAGTTGTTACCAGTGAGCCTTATAGAAGCTCAAATATAAGACCTTCTCCCTTTGAAGAT CTGAATGCCAGAAGAGTCTACATGCAAAGCCAAGCCAATCAGATAACATTTGGTCAGCCA GGCTTGGACATTCAGAGCAGAAGTATGGAATACGTACAGCGGCAAATATCCAAGGAATGT GGAAGCCTAAAGTCCCAAAATAGGGTCCCTTTGAAATCAATCAGACATGTCAGCTTTCAA GATGAGGATGAGATTGTCAGAATAAACCCTCGAGATATCTTAATACGTCGCTATGCAGAC TACAGACATCCTGACATGTGGAAAAATGACTTGGAAAGAGATGATGCTGATTCCAGTATT CAGTTTTCTAAACCAGACAGTAAAAAATCAGACTATCTGTACTCTTGTGGGGATGAGACT AAGTTAAGTTCACCCAAAGACTCTGTGGTATTTAAGACGCAGCCTTCCTCATTAAAAATT AAGAAGTCAAAACGAAGAAAAGAGGATGGTGAACGTTCTCGCTGCGTATACTGCCAGGAA AGGTTTAATCATGAAGAAAATGTCAGGGGAAAATGTCAGGATGCTCCAGACCCTATTAAA AGATGCATATATCAAGTTAGTTGCATGCTCTGTGCAGAGAGCATGTTGTATCATTGTATG TCAGACTCAGAGGGAGATTTTTCTGATCCCTGTTCGTGTGACACTAGCGACGACAAGTTC TGCTTGCGATGGTTAGCCCTGGTAGCTTTGTCTTTCATTGTACCATGTATGTGCTGCTAC GTCCCTTTGAGAATGTGCCATCGCTGTGGTGAGGCATGTGGTTGCTGTGGTGGGAAACAT AAAGCTGCTGGATGA Clone variation with respect to NM_152594.2 25 g=>n;26 a=>n;27 c=>n;28 a=>n;29 a=>n;30 c=>n;291 g=>a;1044 t=>c >OriGene 5' read for NM_152594 unedited
TTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCGGTGCTGCTGTTGCTCC CCCGCCTGCTGTTGCTCCTCCATCTCCAGATCGGATCACGGTGAGGGAAAGATGAGCGAG GAGACGGCGACTTCTGACAACGATAATAGTTATGCACGAGTGCGAGCTGTGGTGATGACC CGAGATGACTCAAGTGGTGGATGGTTACCACTTGGAGGGAGTGGACTAAGCAGCGTCACT GTCTTCAAAGTCCCTCATCAGGAAGAGAATGGCTGTGCTGACTTTTTTATCCGTGGAGAG CGACTCAGGGACAAAATGGTGGTTTTGGAATGTATGCTTAAAAAAGACCTCATTTATAAT AAGGTCACTCCAACATTTCACCACTGGAAGATTGATGACAAAAAGTTTGGTCTTACGTTT CAAAGTCCTGCTGATGCTAGGGCTTTTGATAGAGGTATCCGAAGAGCTATAGAGGATATT TCTCAAGGATGCCCCGAATCAAAAAATGAAGCTGAAGGGGCAGATGACTTACAAGCAAAT GAAGAGGATTCTTCCAGTTCTCTAGTGAAGGATCACCTTTTTCAGCAAGAGACAGTTGTT ACCAGTGAGCCTTATAGAAGCTCAAATATAAGACCTTCTCCCTTTGAAGATCTGAATGCC AGAAGAGTCTACATGCAAAGCCAAGCCAATCAGATAACATTTGGTCAGCCAGGCTTGGAC ATTCAGAGCAGAAGTATGGAATACGTACAGCGGCAAATATCCAAGGGATGGTGGAAAGCC TAAGTCCCAAATAAGGTCCCTTTGAAATCATNAGACATGTCAGCTTTCAGATGAGGATGA GATTGTCAGAATAACCTCGAGATATCTAATACGTCGCTATGCAGACTCAGACATTCTGAC ATGTGAAAAAGACT >OriGene 3' read for NM_152594 unedited
ATCTGTGNNACCGCGGCCGCTATCTAGNATCGAGTTTTTTTTTTTTTTTTTTCATCACCA TAGTTTTTAATGAAGAAACTTGTTTAAAATTGTAAAGGAAAAAATGGGAATGGGACGGCA AAATCTTAGCAGCAAAGTGGTTAAACAAATTGAAAATATTAATGCACAAACATTAAAATA TTAAAGCATATATGTTGCATATAAAATACAGTACAGAACCAGGAGTTGCACTATACTGAT TAGTGCTTAACAGAAGAAATGATTAAATTTGTTCCTCCCAGAAGTATATACACAGTTCAT TTCCACAGCATTTTCCTATATAGCCAGCAAGTTATTTTCTTCAGTTATTCACACCTTGAT CAAACCTGAATTATAAACTTAGCACTTACAAATATGAAAATTCATTCACAAGGAAAAACA GTATTTCCATTTCACCAATAAAAATTTTGAAAGTTAACAGTCTATTCTAGGAAACCAAGT TTAGCTGANAACTTCAGGGATGAAGATCATCTGTTGTAGCAGCATTCAAATATATAAACA GTAAAAATAAGACTTAAAACTGCTGCCTACAGTGTCATGTTTTGGATTTAGTTCATCCAA TTGATTTTTAGTACAAAACTAGAAATACCCTCTTCTTCATAACATCTATAGTTATCAATA TATTTTTCTCTTTTCAATGTGAAATAATACTNTCAGATGATCTATATACACAATGTTGTC AGTTCAAGCTGTCATATAATATAAATGCTTTTCTAAAAAAAGTATTTACAGACAGTGGTA TATACATTGGTCAGTTTGATCTGGGGGCAAAGAAAAAAGCTAACACAGCTTAAGTGTGAT ATATAAAAAGAATGATNCNTAAATGACGTTTAANAACCTGCAATAGAAATGAAGCTNCAA AAGTATTGTGATTTAAATTTTTATACACTCTGAAACTTCAAGCATTAAAAN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_152594 |
| Insert Size | 4600 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_152594.1, NP_689807.1 |
| RefSeq Size | 3816 bp |
| RefSeq ORF | 1335 bp |
| Locus ID | 161742 |
| UniProt ID | Q7Z699 |
| Domains | WH1 |
| Protein Pathways | Jak-STAT signaling pathway |
| Gene Summary | The protein encoded by this gene is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. The encoded protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Striatal microRNA controls cocaine intake through CREB signaling
,null,
Nature
,PubMed ID 20613834
[SPRED1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220714 | SPRED1 (Myc-DDK-tagged)-Human sprouty-related, EVH1 domain containing 1 (SPRED1) |
CNY 3,656.00 |
|
| RC220714L1 | Lenti ORF clone of Human sprouty-related, EVH1 domain containing 1 (SPRED1), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC220714L2 | Lenti ORF clone of Human sprouty-related, EVH1 domain containing 1 (SPRED1), mGFP tagged |
CNY 5,890.00 |
|
| RC220714L3 | Lenti ORF clone of Human sprouty-related, EVH1 domain containing 1 (SPRED1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC220714L4 | Lenti ORF clone of Human sprouty-related, EVH1 domain containing 1 (SPRED1), mGFP tagged |
CNY 5,890.00 |
|
| RG220714 | SPRED1 (tGFP-tagged) - Human sprouty-related, EVH1 domain containing 1 (SPRED1) |
CNY 5,256.00 |
