Fbx32 (FBXO32) (NM_148177) Human Untagged Clone

CAT#: SC101194

FBXO32 (untagged)-Human F-box protein 32 (FBXO32), transcript variant 2



  "NM_148177" in other vectors (6)

CNY 2,400.00

CNY 2,950.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Anti-FBXO32 Rabbit Polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms Fbx32; MAFbx
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC101194 sequence for NM_148177 edited (data generated by NextGen Sequencing)
ATGAATATTTTGGAAAAAGTGGTACTGAAAGTCCTTGAAGACCAGCAAAACATTAGACTA
ATAAGGGAACTACTCCAGACCCTCTACACATCCTTATGTACACTGGTCCAAAGAGTCGGC
AAGTCTGTGCTGGTCGGGAACATTAACATGTGGGTGTATCGGATGGAGACGATTCTCCAC
TGGCAGCAGCAGCTGAACAACATTCAGATCACCAGGCCTGCCTTCAAAGGCCTCACCTTC
ACTGACCTGCCTTTGTGCCTACAACTGAACATCATGCAGAGGCTGAGCGACGGGCGGGAC
CTGGTCAGCCTGGGCCAGGCTGCCCCCGACCTGCACGTGCTCAGCGAAGACCGGCTGCTG
TGGAAGAAACTCTGCCAGTACCACTTCTCCGAGCGGCAGATCCGCAAACGATTAATTCTG
TCAGACAAAGGGCAGCTGGATTGGAAGAAGATGTATTTCAAACTTGTCCGATGTTACCCA
AGGAAAGAGCAGTATGGAGATACCCTTCAGCTCTGCAAACACTGTCACATCCTTTCCTGG
AAGGGCACTGACCATCCGTGCACTGCCAATAACCCAGAGAGCTGCTCCGTTTCACTTTCA
CCCCAGGACTTTATCAACTTGTTCAAGTTCTGA

Clone variation with respect to NM_148177.2
>OriGene 5' read for NM_148177 unedited
TTGATACATCCTTAGGCGGCCGGAATCGCCGAGGTGGCATCGCCCAAAAAACTATGAATA
TTTTGGAAAAGTGGTACTGAAAGTCCTTGAAGACCAGCAAAACATTAGACTAATAAGGGA
ACTACTCCAGACCCTCTACACATCCTTATGTACACTGGTCCAAAGAGTCGGCAAGTCTGT
GCTGGTCGGGAACATTAACATGTGGGTGTATCGGATGGAGACGATTCTCCACTGGCAGCA
GCAGCTGAACAACATTCAGATCACCAGGCCTGCCTTCAAAGGCCTCACCTTCACTGACCT
GCCTTTGTGCCTACAACTGAACATCATGCAGAGGCTGAGCGACGGGCGGGACCTGGTCAG
CCTGGGCCAGGCTGCCCCCGACCTGCACGTGCTCAGCGAAGACCGGCTGCTGTGGAAGAA
ACTCTGCCAGTACCACTTCTCCGAGCGGCAGATCCGCAAACGATTAATTCTGTCAGACAA
AGGGCAGCTGGATTGGAAGAAGATGTATTTCAAACTTGTCCGATGTTACCCAAGGAAAGA
GCAGTATGGAGATACCCTTCAGCTCTGCAAACACTGTCACATCCTTTCCTGGAAGGGCAC
TGACCATCCGTGCACTGCCAATAACCCAGAGAGCTGCTCCGTTTCACTTTCACCCCAGGA
CTTTATCAACTTGNTCAAGTTCTGAATCCCAGCACATGACAACACTTCAGAAAGGTCCCC
CTGCTGACTGGAGAGCTGGGAATATGGCATTTGGACACTTCATTTGTAATAGTGTACATT
TTAAACATTGGCTCGAAACTTCANAGATAAGTCATGGAGAAGACATTGNAGGGGAGAAAT
GCATTGCTGACTGGGAAATTAAGAAG
>OriGene 3' read for NM_148177 unedited
TGGTGTGNATTGGCCCCNGTTAGGGATTTNCTAGTTATTTTTTTTTTTTTTTCTAAACAA
ATTTTCACATTGTAAACAAAGCGTCCTATTTTGTATTTGAGTTTTGCCTTTTTCTTCCTA
GGGAACTATAAGAGAGTTTCTGTGACCATTAAGAGGACTCCATTTTCACCGCTGAAATAG
AATTATTGCCCTTATAGTCTTGCTGGCAAATTGTTAGAAAAAAAGTTTATTTACAGTATT
TTGCTTTTCCATACCAATTCTAGTGAGAAGTTCACATTCTTAAATTCCCAGTCAGCAACT
GCATTTCTCCCCTCCAATGTCCTCTCCATGACTTATCTCTGAAGTTTCGAGCCAATGTTT
AAAATGTACACTATTTACAAATGAAGTGTCCAAATGCCATATTCCCAGCTCTCCAGTCAG
CAGGGGGACCCTTCTGAAGTGTTGTCATGTGCTGGGATTCAGAACTTGAACAAGTTGATA
AAGTCCTGGGGTGAAAGTGAAACGGAGCAGCTCTCTGGGTTATTGGCAGTGCACGGATGG
TCAGTGCCCTTCCAGGAAAGGATGTGACAGTGTTTGCAGAGCTGAAGGGTATCTCCATAC
TGCTCTTTCCTTGGGTAACATCGGACAAGTTTGAAATACATCTTCTTCCAATCCAGCTGC
CCTTTGTCTGACAGAATTAATCGTTTGCGGATCTGCCGCTCGGAGAAGTGGTACTGGCAG
AGTTTCTTCCACAGCAGCCGGTCTTCCCTGAGCACGTGCAGGTCGGGGGCAGCCTGGCCC
AGGCTGACCAGGTCCCGCCCGTCGCTCAGCCTCTGCATGATGTTCAGGTGGAGGGCACAA
AGCCGGTCATTGGAAGGGAGGCCCTTTAAGGCAGGCCTGGGGATCTGAATGTTGTTCAAC
TGCTGCTGCCAGTGGGAAATTCTCCTCCTTCCGATAACCCCCAGTTGAAG
Restriction Sites NotI-NotI     
ACCN NM_148177
Insert Size 1150 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_148177.1, NP_680482.1
RefSeq Size 1212 bp
RefSeq ORF 633 bp
Locus ID 114907
Domains F-box
Gene Summary This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
Transcript Variant: This variant (2) has a shorter and alternate 5' terminal sequence lacking an in-frame translation start codon, as compared to variant 1. It uses a downstream in-frame translation start codon and encodes a shorter isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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