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ZC3HAV1 (NM_024625) Human Untagged Clone
CAT＃: SC101064
ZC3HAV1 (untagged)-Human zinc finger CCCH-type, antiviral 1 (ZC3HAV1), transcript variant 2
CNY 7,688.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ARTD13; FLB6421; PARP13; ZAP; ZC3H2; ZC3HDC2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC101064 sequence for NM_024625 edited (data generated by NextGen Sequencing)
ATGGCGGACCCGGAGGTGTGCTGCTTCATCACCAAAATCCTGTGCGCCCACGGGGGCCGC ATGGCCCTGGACGCGCTGCTCCAGGAGATCGCGCTGTCTGAGCCGCAGCTCTGTGAGGTG CTGCAGGTGGCCGGGCCCGACCGCTTTGTGGTGTTGGAGACCGGCGGCGAGGCCGGGATC ACCCGATCGGTGGTGGCCACCACTCGAGCCCGGGTCTGCCGTCGCAAGTACTGCCAGAGA CCCTGCGATAACCTGCATCTCTGCAAACTCAACTTGCTGGGCCGGTGCAACTATTCGCAG TCCGAGCGGAATTTATGCAAATATTCTCATGAGGTTCTCTCAGAAGAGAACTTCAAAGTC CTGAAAAATCACGAACTCTCTGGACTGAACAAAGAGGAATTAGCAGTGCTCCTCCTCCAA AGTGATCCTTTTTTTATGCCCGAGATATGCAAAAGTTATAAGGGAGAGGGTCGGCAGCAG ATTTGTAACCAGCAGCCACCGTGTTCAAGACTCCACATCTGTGACCACTTCACCCGAGGG AACTGTCGTTTTCCCAACTGCCTCCGGTCCCATAACCTGATGGACAGAAAGGTGCTGGCC ATCATGAGGGAGCACGGGCTGAACCCCGACGTGGTCCAGAACATCCAGGACATCTGCAAC AGCAAGCACATGCAGAAGAATCCCCCAGGGCCCAGAGCTCCTTCTTCACATCGTAGAAAC ATGGCATATAGGGCTAGAAGCAAGAGTAGAGATCGGTTCTTTCAGGGCAGCCAAGAATTT CTTGCGTCTGCTTCAGCGTCTGCTGAGAGGTCCTGCACACCTAGTCCAGATCAGATCAGC CACAGGGCTTCCCTGGAGGACGCGCCTGTGGACGATCTCACCCGCAAGTTCACGTATCTG GGGAGTCAGGATCGCGCTCGGCCTCCCTCAGGCTCGTCCAAGGCTACTGATCTTGGAGGA ACAAGTCAGGCCGGGACAAGCCAGAGGTTTTTAGAGAACGGCAGTCAAGAGGACCTCTTG CATGGAAATCCAGGCAGCACTTACCTTGCTTCCAATTCAACATCAGCCCCCAACTGGAAG AGCCTCACATCCTGGACGAATGACCAAGGCGCCAGGAGAAAGACTGTGTTTTCTCCCACG CTACCTGCCGCCCGCTCTTCTCTTGGCTCTCTGCAAACACCTGAAGCTGTGACCACCAGA AAGGGCACAGGCTTGCTTTCCTCAGACTACAGGATCATCAATGGCAAAAGTGGAACTCAG GACATCCAGCCTGGCCCTCTTTTTAATAATAATGCTGATGGAGTGGCCACAGATATAACT TCTACCAGATCCTTAAATTACAAAAGCACTAGCAGCGGTCACAGAGAAATATCATCACCT AGGATTCAGGATGCTGGACCTGCTTCCCGAGATGTCCAGGCCACTGGCAGAATCGCAGAT GATGCTGACCCAAAAGTAGCACTTGTTAACGATTCTTTATCTGATGTCACAAGTACCACA TCTTCTAGGGTGGATGATCATGACTCAGAGGAAATTTGTCTTGACCATCTGTGTAAGGGT TGTCCGCTTAATGGTAGCTGCAGCAAAGTCCACTTCCATCTGCCTTACCGGTGGCAGATG CTTATTGGTAAAACCTGGACGGACTTTGAGCACATGGAGACGATCGAGAAAGGCTACTGT AACCCCGGAATCCAGCTCTGTTCTGTAGGAAGTTATACAATCAATTTTCGGGTAATGAGT TGTGATTCCTTTCCCATCCGACGCCTCTCCACTCCTTCTTCTGTCACCAAGCCAGCCAAT TCTGTCTTCACCACCAAATGGATTTGGTATTGGAAGAATGAATCTGGCACATGGATTCAG TATGGAGAAGAGAAAGACAAACGGAAAAATTCAAACGTCGACTCTTCATACCTGGAGTCT CTCTATCAATCCTGTCCGAGGGGAGTTGTGCCATTTCAGGCGGGCTCACGGAACTATGAG CTGAGTTTCCAAGGGATGATTCAGACAAACATAGCTTCCAAAACTCAAAAGGATGTCATC AGAAGACCAACATTTGTGCCTCAGTGGTATGTGCAGCAGATGAAGAGAGGGCCAGAGTAA Clone variation with respect to NM_024625.3 1454 g=>a;1695 c=>g >OriGene 5' read for NM_024625 unedited
AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCCCGCGCCGCGGCAGAG CCAGCACAGCCAGCGCGCCATGGCGGACCCGGAGGTGTGCTGCTTCATCACCAAAATCCT GTGCGCCCACGGGGGCCGCATGGCCCTGGACGCGCTGCTCCAGGAGATCGCGCTGTCTGA GCCGCAGCTCTGTGAGGTGCTGCAGGTGGCCGGGCCCGACCGCTTTGTGGTGTTGGAGAC CGGCGGCGAGGCCGGGATCACCCGATCGGTGGTGGCCACCACTCGAGCCCGGGTCTGCCG TCGCAAGTACTGCCAGAGACCCTGCGATAACCTGCATCTCTGCAAACTCAACTTGCTGGG CCGGTGCAACTATTCGCAGTCCGAGCGGAATTTATGCAAATATTCTCATGAGGTTCTCTC AGAAGAGAACTTCAAAGTCCTGAAAAATCACGAACTCTCTGGACTGAACAAAGAGGAATT AGCAGTGCTCCTCCTCCAAAGTGATCCTTTTTTTATGCCCGAGATATGCAAAAGTTATAA GGGAGAGGGTCGGCAGCAGATTTGTAACCAGCAGCCACCGTGTTCAAGACTCCACATCTG TGACCACTTCACCCGAGGGAACTGTCGTTTTCCCAACTGCCTCCGGTCCCATAACCTGAT GGACAGAAAGGTGCTGGCCATCATGAGGGAGCACGGGCTGAACCCCGACGTGGTCCAGAA TCATCCAGGACATCTGCACAGCAAGCACATGCAGAAGAATCCCCCAGGGCCAGAGCTNCT TCNTCACATCGTAAAAACATGGCATATAGGGCTAGAAGCAGAGTAGAGATCGGNTCTTTC AGGGCAGNNCAGATTNCTTGCGTCTGCTTCANCGCTGCTGAGAGGTCTGCACACTAGTCA GATCAGACAGCACAGGNCTCCCCTGAGACGCGCTGTTGCGATCTACCGCAGTCACTTCCT TGAG >OriGene 3' read for NM_024625 unedited
GGAGAGTCGCGGGGTGGGGGGCGGCGGGAAATTTTGGGGTTTCGCNCTTTNCCCCCCNNN NCCNNNNCGCGGCCCCGGTTTTGGTCCCCCTGTTCCTTTTCCGGGGGGCGTTACAAGGAA CCCCCCCGGCCACGTTTCCTTTAAAATCCCTTCAATCGGATTATCTCTCACCAAAACCCT TGCTCTAACAGCTAGCTCATGCCCTTTTTTCTATCTCTTTGTCGGGCCCTCATCCTTTAT AACATCTTGTAGCGAAAACAAGAGGACACCGCAAAAAAATCCACAGCAAAGCCAGGCACG GTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGACTGAGGTAGGAGGATCACTTGAGCT CAGGAGTTCAAGACCAGCCTGGGCAACAAAGTGAGACCCCCTCTAAAAAAAAAAAAAAAA AAAAAGAATCCACAGCAAAACAAAAACCCAGTATATATGATTATTCCTTAGGGGTACCGG CATCAATCCAGCTTTACCAGCCGGAAAAGCTAAAGCCCAGAGTAGTTAGGAAACTCGCTC AGACTTCCGTTTGAAAAAGAAAACACGTGCCCGATCTAGTATCCTTTCAGAACACACCAT GCTGGCTTAACAAACATCCTTAATTCACGCATAATGCCATACGCGAAACCGGCCAGAGAA GGGCCCACCAGGAACTTTATCGGAAAAACAGGCCCCAGAAAGCCGAGAAGGGAATTGGTG AAGCCCCCGGGAACGGAAGAAGCTAAAGCAATAGTGACAACCAAACAACGGGCTCCAGAA AAACATTACAGGGGGCCCTGTCTTACATCGGGGGGGACATCCACTGGAAGGCCAAAGTGT GAGTCACGAGAGAAAAACCCCTTGAAGTTCTGGAAAGCCTTGTGGTGACGGGAACAACCC CGAGGAGAAACGACTGTATAATTCACGGGGAACCGACATAGATGGGCCGGCCG |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_024625 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_024625.3, NP_078901.3 |
| RefSeq Size | 3218 bp |
| RefSeq ORF | 2100 bp |
| Locus ID | 56829 |
| UniProt ID | Q7Z2W4 |
| Domains | zf-CCCH |
| Gene Summary | This gene encodes a CCCH-type zinc finger protein that is thought to prevent infection by retroviruses. Studies of the rat homolog indicate that the protein may primarily function to inhibit viral gene expression and induce an innate immunity to viral infection. Alternative splicing occurs at this locus and two variants, each encoding distinct isoforms, are described. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region and lacks several downstream exons, compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus compared to isoform 1. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The Zinc-Finger Antiviral Protein ZAP Inhibits LINE and Alu Retrotransposition
,Moldovan, JB;Moran, JV;,
PLoS Genet.
,PubMed ID 25951186
[ZC3HAV1]
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Other Versions
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| RC208070L2 | Lenti-ORF clone of ZC3HAV1 (mGFP-tagged)-Human zinc finger CCCH-type, antiviral 1 (ZC3HAV1), transcript variant 2 |
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| RC208070L3 | Lenti-ORF clone of ZC3HAV1 (Myc-DDK-tagged)-Human zinc finger CCCH-type, antiviral 1 (ZC3HAV1), transcript variant 2 |
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| RC208070L4 | Lenti-ORF clone of ZC3HAV1 (mGFP-tagged)-Human zinc finger CCCH-type, antiviral 1 (ZC3HAV1), transcript variant 2 |
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| RG208070 | ZC3HAV1 (tGFP-tagged) - Human zinc finger CCCH-type, antiviral 1 (ZC3HAV1), transcript variant 2 |
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| SC324088 | ZC3HAV1 (untagged)-Human zinc finger CCCH-type, antiviral 1 (ZC3HAV1), transcript variant 2 |
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