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RNF185 (NM_152267) Human Untagged Clone
CAT＃: SC100933
RNF185 (untagged)-Human ring finger protein 185 (RNF185), transcript variant 1
CNY 2,400.00
CNY 2,950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_152267, the custom clone sequence may differ by one or more nucleotides
ATGGCAAGCAAGGGGCCCTCGGCCTCTGCATCTCCTGAGAACTCCAGTGCAGGGGGGCCCAGTGGGAGCA GCAATGGCGCTGGCGAGAGCGGAGGGCAGGACAGCACTTTCGAGTGCAACATCTGCTTGGACACAGCCAA GGATGCCGTCATCAGCCTGTGTGGCCACCTCTTCTGTTGGCCGTGTTTACATCAGTGGTTGGAGACCAGA CCTAACAGACAGGTGTGTCCTGTTTGCAAAGCTGGCATCAGCCGAGACAAGGTCATCCCCCTCTATGGAA GGGGCAGCACTGGGCAACAGGACCCCAGAGAGAAGACCCCTCCTCGTCCTCAAGGACAGAGGCCAGAGCC GGAGAATAGAGGGGGATTTCAAGGATTTGGATTTGGAGATGGTGGCTTCCAGATGTCTTTTGGAATTGGG GCATTTCCCTTTGGGATATTTGCCACAGCATTTAATATAAATGATGGGCGGCCTCCTCCAGCTGTCCCTG GGACACCCCAGTATGTGGACGAGCAGTTCCTGTCACGCCTCTTCCTATTTGTGGCCCTGGTGATCATGTT CTGGCTCCTGATTGCCTAA >OriGene 5' read for NM_152267 unedited
ATTCACCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGC AGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCG AATTCGGCACGAGGGTCGGAGGTCTTACCCAACAGATTGACGCGGCGTTAGTATTGGCCG TGTNACCCGAAAAACTGATTGACTGGGCTGGCGTTAACTGTGCGGAGGCAGGATTTCCCT GGGGAAAGTCTTCACTCAGAGCTTCGCTGACAGCCAAGGATGGCAAGCAAGGGGCCCTCG GCCTCTGCATCTCCTGAGAACTCCAGTGCAGGGGGGCCCAGTGGGAGCAGCAATGGCGCT GGCGAGAGCGGAGGGCAGGACAGCACTTTCGAGTGCAACATCTGCTTGGACACAGCCAAG GATGCCGTCATCAGCCTGTGTGGCCACCTCTTCTGTTGGCCGTGTTTACATCAGGGATTT CAAGGATTTGGATTTGGAGATGGTGGCTTCCAGATGTCTTTTGGAATTGGGGCATTTCCC TTTGGGATATTTGCCACAGCATTTAATATAAATGATGGGCGGCCTCCTCCAGCTGTCCCT GGGACACCCCAGTATGTGGACGAGCAGTTCCTGTCACGCCTCTTCCTATTTGTGGCCCTG GTGATCATGTTCTGGCTCCTGATTGCCTAATGCTGGGCTCCTGCCTACATCCGTGGCAGG GCTCTGGACTGGTGACGTGCCACCCCAACTCCTGGTGTTTGGCTTCCTGGCTAATCTTGA CTCCTGGGATCAGTGGGATCAGTAACACATCAAGGAGTCTTGTTTCTTCATCAGAGCTTT GGGACTCGAGACCAGTTGGCGATGACCCCTNGATATCGCCACCGCTGTAAACACTCTATA ACTTCAGGGCCTGGCATTGAGTCATCTCTC >OriGene 3' read for NM_152267 unedited
GAATTCTATGTNACCGCGGCCGCAATTCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATGGAAAACCCTTTGTTTAATTCTAAAT CAAATCACTTTCACAACAGTGAAAATTAGTGACTGGTTAAGGGGGGCCACTGGACATATC ATCATTTTCTGACTGGGGTCAGGACCTGGTCCTAATCCACTTTTTTTGCAAGAGGAGGGT GGAGGCTAAGAACCCCCAAAACCCCCCAAAAAAAGGAAAGCTGCCTTGGCAAAAGGATGA AGTGGTGAACTTGCCCAGGGATGGTGGGAAAGGGGCTCCCTGTTGGGGCCCAGCCAGGAG TCCCAAGTCAGCTCTCCTGCCTTACTTAACTCCTGGCAAAGGGGGAGTGGGGACCCACCA AGTTCAAAATCAAATGGCATTTGGCCCGCCTGGCTTTACTAACAGGTTCCCAAAGTGCCC CTGTTGGCTGAGCTCTCCTGGGCTCACTCCCTTTCATTGAAGAATCCCAATGATTCATTT TCCTACCCACAACTTTTTATTATTTTTTTGGAAACCCCTTTTTGGTGAGGCCCTCTGACT TAAGTCCTCTCTCCCTCCACCTATTGGGGCCCCTGCCCTGAGGGGGGGCCCACCAATTTT CTTTTAAAAAGAAACACCCCCAAAGCCCCTGCCAATTTGCGGGTTTTCCCAAAGGGGAAA AAAAAACCCCCTTTAAGGGGTGGCCCCGAATAGTTTTAAATCTTTCCGGCCCCCTTTTAA ACCGGGGGGTGTTTTCCCACCCCAAGGGTTTTGCCCTTTAACACCCCCNCCCCAAACCTT ATTTTTTTGGGCGGTTAAAACCCACGAACCCTGGGTTTGACCAGGCCCAAAAGAAAAAAA ATGGGGGCCTCTCAAAAGAGGGGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_152267 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_152267.2, NP_689480.2 |
| RefSeq Size | 3240 bp |
| RefSeq ORF | 579 bp |
| Locus ID | 91445 |
| UniProt ID | Q96GF1 |
| Domains | RING |
| Protein Families | Druggable Genome, Transmembrane |
| Gene Summary | E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating 'Lys-63'-linked polyubiquitination of BNIP1 (PubMed:21931693). Acts in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent proteasome-mediated degradation (PubMed:27485036). Protects cells from ER stress-induced apoptosis (PubMed:27485036). Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway (PubMed:24019521). Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2 (PubMed:24019521).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the predominant transcript and encodes the longer isoform (1). |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207827 | RNF185 (Myc-DDK-tagged)-Human ring finger protein 185 (RNF185), transcript variant 1 |
CNY 2,400.00 |
|
| RC207827L3 | Lenti ORF clone of Human ring finger protein 185 (RNF185), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207827L4 | Lenti ORF clone of Human ring finger protein 185 (RNF185), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG207827 | RNF185 (tGFP-tagged) - Human ring finger protein 185 (RNF185), transcript variant 1 |
CNY 4,000.00 |
