RASGRP 4 (RASGRP4) (NM_170603) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	guanyl nucleotide releasing protein 4; RAS guanyl releasing protein 4
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC100713 sequence for NM_170603 edited (data generated by NextGen Sequencing) ATGAACAGAAAAGACAGTAAGAGGAAGTCCCACCAGGAATGCACCGGAAAAACAGGAGGG CGAGGCCGGCCCCGCCAAGTGCGCCGCCACAAGACATGCCCCAGCCCTCGGGAAATCAGC AAGGTCATGGCTTCCATGAACCTGGGCCTGCTGAGTGAGGGCGGCTGCAGCGAAGATGAG CTGCTGGAGAAATGCATCCAGTCCTTCGATTCAGCTGGCAGCCTGTGCCACGAGGACCAC ATGCTCAACATGGTGCTGGCCATGCACAGCTGGGTGCTGCCGTCCGCCGACCTGGCTGCC CGCCTGCTGACCTCATACCAGAAGGCCACAGGGGACACCCAGGAGCTGAGACGGCTGCAG ATCTGTCACCTGGTCAGGTACTGGCTGATGCGACACCCTGAGGTGATGCACCAGGATCCC CAGCTAGAAGAAGTCATAGGTCGTTTCTGGGCCACCGTGGCCCGGGAGGGCAACTCAGCC CAGAGAAGACTGGGAGACTCTTCTGACCTCCTGAGCCCTGGTGGCCCTGGCCCCCCACTC CCAATGAGCAGCCCAGGCCTGGGCAAAAAGCGCAAAGTGTCCTTGCTTTTCGACCACTTG GAGACGGGGGAGCTGGCTCAGCACCTCACCTACCTGGAGTTCCGGTCCTTCCAGGCTATC ACGCCCCAGGACCTGCGGAGCTACGTTTTGCAGGGCTCAGTACGAGGCTGCCCGGCCCTG GAGGGCTCCGTAGGTCTCAGCAACAGCGTGTCCCGCTGGGTGCAGGTGATGGTGCTGAGC CGTCCCGGGCCCCTACAGCGTGCACAGGTGCTGGACAAGTTCATTCACGTGGCACAGAGG CTCCACCAGCTGCAGAATTTCAACACGCTGATGGCAGTCACAGGGGGCCTGTGTCACAGT GCCATCTCCAGACTCAAGGACTCCCATGCCCACCTGAGCCCTGACAGCACCAAGGCCCTC CTGGAGCTCACTGAGCTCCTTGCCTCCCACAACAACTACGCCCGCTACCGCCGCACCTGG GCTGGCTGCGCGGGTTTCCGGCTGCCTGTACTGGGCGTGCACCTCAAGGACCTGGTGTCC CTGCATGAGGCACAGCCCGACAGGTTGCCTGACGGCCGCCTGCACCTACCCAAGCTGAAC AACCTCTACCTGCGGCTGCAGGAGCTGGTGGCCCTCCAAGGGCAGCATCCACCCTGCAGC GCCAATGAGGATCTGCTGCACCTGCTCACGCTCTCCCTGGACCTCTTCTACACGGAAGAC GAGATCTATGAGCTTTCTTATGCCCGGGAGCCGCGTTGTCCCAAGAGCCTGCCACCCTCC CCCTTCAATGCACCTCTGGTGGTGGAGTGGGCCCCTGGTGTGACACCCAAGCCGGACAGG GTCACACTGGGTCGGCATGTGGAGCAGCTGGTGGAGTCTGTGTTCAAGAATTATGACCCT GAAGGCCGAGGAACAATCTCTCAGGAGGACTTTGAGCGACTCTCGGGCAATTTTCCCTTC GCCTGCCATGGGCTTCACCCACCCCCACGCCAGGGGAGAGGATCCTTCAGCAGAGAGGAG CTGACAGGGTACCTGCTCCGGGCCAGCGCCATCTGCTCCAAGTTGGGCCTGGCCTTCCTG CACACCTTCCATGAGGTCACCTTCCGAAAGCCTACCTTCTGCGACAGCTGCAGTGGCTTC CTCTGGGGTGTCACCAAGCAAGGCTACCGCTGTCGGGAGTGCGGGCTGTGTTGCCACAAA CACTGCAGAGACCAGGTGAAGGTAGAATGTAAGAAGAGGCCAGGGGCCAAGGGCGATGCA GGACCCCCCGGAGCTCCTGTCCCATCCACACCAGCTCCCCATGCCAGCTGTGGCTCCGAG GAAAATCACTCCTACACGCTATCCCTGGAGCCTGAGACTGGGTGCCAGCTTCGCCATGCC TGGACCCAGACTGAATCCCCACACCCTTCCTGGGAAACAGATACGGTCCCCTGCCCGGTG ATGGACCCACCATCAACTGCATCCTCCAAGCTGGATTCCTAG Clone variation with respect to NM_170603.1 53 t=>c 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_170603 unedited CTCTGGAATTTGTAATACGACTTACTATAGNNGGCGGCCGCGCAATTCGGCACCAGGCGG GAAGCATGAACAGAAAAGACAGTAAGAGGAAGTCCCACCAGGAATGCACCGGAAAAACAG GAGGGCGAGGCCGGCCCCGCCAAGTGCGCCGCCACAAGACATGCCCCAGCCCTCGGGAAA TCAGCAAGGTCATGGCTTCCATGAACCTGGGCCTGCTGAGTGAGGGCGGCTGCAGCGAAG ATGAGCTGCTGGAGAAATGCATCCAGTCCTTCGATTCAGCTGGCAGCCTGTGCCACGAGG ACCACATGCTCAACATGGTGCTGGCCATGCACAGCTGGGTGCTGCCGTCCGCCGACCTGG CTGCCCGCCTGCTGACCTCATACCAGAAGGCCACAGGGGACACCCAGGAGCTGAGACGGC TGCAGATCTGTCACCTGGTCAGGTACTGGCTGATGCGACACCCTGAGGTGATGCACCAGG ATCCCCAGCTAGAAGAAGTCATAGGTCGTTTCTGGGCCACCGTGGCCCGGGAGGGCAACT CAGCCCAGAGAAGACTGGGAGACTCTTCTGACCTCCTGAGCCCTGGTGGCCCTGGCCCCC CACTCCCAATGAGCAGCCCAGGCCTGGGCAAAAGCGCANAGTGTCCTTGCTTTTCGACCC TTGGAGACGGGGGAGCTGGCTCAGCACCTCACCTACCTGGAGTTCCGGTCCTTNNCAGCT ATCACGCCCCAGGACCTGCGAGCTACGTTTTGCAGGGCTCAGTACGAGGCTGCCCCGCCC TGGAGGGCTCCGTAAGTCTCAGCACAGCGTGTCCCNGCTGGTGCAGTGATGGTGCTGANC CCNTCCGGGCCCNTACACGTGCACAGTGCTGGACAGTTCATTCCTTGCACAGAGGCTCCA CAGCTGCCGATTCCACCCCTGATGGCATAAAGGGGGCCTGGTCCAATGGCATTTCAAA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_170603 unedited GAAAATAGTGAATTTNAATTTCGNACATCTGTGGGTACCTCTCTCTGTGGGGGCCAATCG GGGTGTTGGGGTTTGTGAAGGGGTTGGGGGGGTCTCTGCTCCCTGGGACTGAATGGGCCC CTGCTGCTCGACCCTCAAAGTTCTTTGCCCTATAGCACATCTATCCTGTGCCGTGGGCAT GGGTAAGGAGACATTCAGGCATCAGACAAGCATCTGTCTTGTTCCTACCACCTGCCAGCC CTGTTCTAGGCACTGGGGATGCAGCAGTGAATGACATTGATAGAAATCCCTGTTCTCCTG GAGTTGATGCTCCTGTTGAGGAAAGACAGACGTTCAACGAGATAAAGAGGGAAAAAGTAT AGGCTGTCAGAGGGAGCACATTCTATGCAGAAAAACAGGAGCTGGGCGTGGTGGCTCACG CCTGTATTCCCAGCACTTTGGGAGGCTGAGGCTGGTGGATCATGAGGTCAGGAGTTTGAG ACCATCTTGGCCAACATGGGGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGGC ATGGTGGCGCGTGCCTGTAATCCCAGCTACTTGCGAGGCTGAGGCAGAAGAATCACTTTG ACCCAGNGAGCGGAAGTTGCAGTGAGCTGAGATTGCATCATTGCACTCCAGCCTGGGTGA CAGAGCAAGACTCTGTCTAAAAAAAAAAAGAAATGGAATGTAGGGAGTTGGTAAGAATAA GCCCTGAGTCTTAATGTTTTCAACACACAGGCCGACAGAATCCTAAATGCAGGGGAAAGG GAGCACGGGATTACCATCCCCCAGGATGCCAAATAAGAAAGTCACTTCCCGTGGAAAAAG GAGACCGGCCTCCCACCTTAAAGCCACTTTGGATTGGGAAAGCCGGTTGCCTTTGGGGGC CCTCCTTTTGGGAACCCTGCCAAAATTTACCGCAGGACCTAGACCTGACGGG
Restriction Sites	NotI-NotI
ACCN	NM_170603
Insert Size	3000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_170603.1, NP_733748.1
RefSeq Size	2634 bp
RefSeq ORF	2634 bp
Locus ID	115727
Domains	RasGEF, DAG_PE-bind
Protein Families	Druggable Genome
Protein Pathways	MAPK signaling pathway
Gene Summary	The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009] Transcript Variant: This variant (1) encodes the longest isoform (1).

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