NCOA1 (NM_147223) Human Untagged Clone
CAT#: SC100652
NCOA1 (untagged)-Human nuclear receptor coactivator 1 (NCOA1), transcript variant 2
CNY 9,576.00
Cited in 1 publication. |
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | bHLHe42; bHLHe74; F-SRC-1; KAT13A; RIP160; SRC1 |
Vector | pCMV6-XL6 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_147223, the custom clone sequence may differ by one or more nucleotides
ATGAGTGGCCTCGGGGACAGTTCATCCGACCCTGCTAACCCAGACTCACATAAGAGGAAAGGATCGCCAT GTGACACACTGGCATCAAGCACGGAAAAGAGGCGCAGGGAGCAAGAAAATAAATATTTAGAAGAACTAGC TGAGTTACTGTCTGCCAACATTAGTGACATTGACAGCTTGAGTGTAAAACCAGACAAATGCAAGATTTTG AAGAAAACAGTCGATCAGATACAGCTAATGAAGAGAATGGAACAAGAGAAATCAACAACTGATGACGATG TACAGAAATCAGACATCTCATCAAGTAGTCAAGGAGTGATAGAAAAGGAATCCTTGGGACCTCTTCTTTT GGAGGCTTTGGATGGATTTTTCTTTGTTGTGAACTGTGAAGGGAGAATTGTATTTGTGTCAGAGAATGTA ACCAGCTACTTAGGTTACAATCAGGAGGAATTAATGAATACGAGCGTCTACAGCATACTGCACGTGGGGG ATCATGCAGAATTTGTGAAGAATCTGCTACCAAAATCACTAGTAAATGGAGTTCCTTGGCCTCAAGAGGC AACACGACGAAATAGCCATACCTTTAACTGCAGGATGCTAATTCACCCTCCAGATGAGCCAGGGACCGAG AACCAAGAAGCTTGCCAGCGTTATGAAGTAATGCAGTGTTTCACTGTGTCACAGCCAAAATCAATTCAAG AGGATGGAGAAGATTTCCAGTCATGTCTGATTTGTATTGCACGGCGATTACCTCGGCCTCCAGCTATTAC GGGTGTAGAATCCTTTATGACCAAGCAAGATACTACAGGTAAAATCATCTCTATTGATACTAGTTCCCTG AGAGCTGCTGGCAGAACTGGTTGGGAAGATTTAGTGAGGAAGTGCATTTATGCTTTTTTCCAACCTCAGG GCAGAGAACCATCTTATGCCAGACAGCTGTTCCAAGAAGTGATGACTCGTGGCACTGCCTCCAGCCCCTC CTATAGATTCATATTGAATGATGGGACAATGCTTAGCGCCCACACCAAGTGTAAACTTTGCTACCCTCAA AGTCCAGACATGCAACCTTTCATCATGGGAATTCATATCATCGACAGGGAGCACAGTGGGCTTTCTCCTC AAGATGACACTAATTCTGGAATGTCAATTCCCCGAGTAAATCCCTCGGTCAATCCTAGTATCTCTCCAGC TCATGGTGTGGCTCGTTCATCCACATTGCCACCATCCAACAGCAACATGGTATCCACCAGAATAAACCGC CAGCAGAGCTCAGACCTTCATAGCAGCAGTCATAGTAATTCTAGCAACAGCCAAGGAAGTTTCGGATGCT CACCCGGAAGTCAGATTGTAGCCAATGTTGCCTTAAACCAAGGACAGGCCAGTTCACAGAGCAGTAATCC CTCTTTAAACCTCAATAATTCTCCTATGGAAGGTACAGGAATATCCCTAGCACAGTTCATGTCTCCAAGG AGACAGGTTACTTCTGGATTGGCAACAAGGCCCAGGATGCCAAACAATTCCTTTCCTCCTAATATTTCGA CATTAAGCTCTCCCGTTGGCATGACAAGTAGTGCCTGTAATAATAATAACCGATCTTATTCAAACATCCC AGTAACATCTTTACAGGGTATGAATGAAGGACCCAATAACTCCGTTGGCTTCTCTGCCAGTTCTCCAGTC CTCAGGCAGATGAGCTCACAGAATTCACCTAGCAGATTAAATATACAACCAGCAAAAGCTGAGTCCAAAG ATAACAAAGAGATTGCCTCAATTTTAAATGAAATGATTCAATCTGACAACAGCTCTAGTGATGGCAAACC TCTGGATTCAGGGCTTCTGCATAACAATGACAGACTTTCAGATGGAGACAGTAAATACTCTCAAACCAGT CACAAACTAGTGCAGCTTTTGACAACAACTGCCGAACAGCAGTTACGGCATGCTGATATAGACACAAGCT GCAAAGATGTCCTGTCTTGCACAGGCACTTCCAACTCTGCCTCTGCTAACTCTTCAGGAGGTTCTTGTCC CTCTTCTCATAGCTCATTGACAGAACGGCATAAAATTCTACACCGGCTCTTACAGGAGGGTAGCCCCTCA GATATCACCACTTTGTCTGTCGAGCCTGATAAAAAGGACAGTGCATCTACTTCTGTGTCAGTGACTGGAC AGGTACAAGGAAACTCCAGTATAAAACTAGAACTGGATGCTTCAAAGAAAAAAGAATCAAAAGACCATCA GCTCCTACGCTATCTTTTAGATAAAGATGAGAAAGATTTAAGATCAACTCCAAACCTGAGCCTGGATGAT GTAAAGGTGAAAGTGGAAAAGAAAGAACAGATGGATCCATGTAATACAAACCCAACCCCAATGACCAAAC CCACTCCTGAGGAAATAAAACTGGAGGCCCAGAGCCAGTTTACAGCTGACCTTGACCAGTTTGATCAGTT ACTGCCCACGCTGGAGAAGGCAGCACAGTTGCCAGGCTTATGTGAGACAGACAGGATGGATGGTGCGGTC ACCAGTGTAACCATCAAATCGGAGATCCTGCCAGCTTCACTTCAGTCCGCCACTGCCAGACCCACTTCCA GGCTAAATAGATTACCTGAGCTGGAATTGGAAGCAATTGATAACCAATTTGGACAACCAGGAACAGGCGA TCAGATTCCATGGACAAATAATACAGTGACAGCTATAAATCAGAGTAAATCAGAAGACCAGTGTATTAGC TCACAATTAGATGAGCTTCTCTGTCCACCCACAACAGTAGAAGGGAGAAATGATGAGAAGGCTCTTCTTG AACAGCTGGTATCCTTCCTTAGTGGCAAAGATGAAACTGAGCTAGCTGAACTAGACAGAGCTCTGGGAAT TGACAAACTTGTTCAGGGGGGTGGATTAGATGTATTATCAGAGAGATTTCCACCACAACAAGCAACGCCA CCTTTGATCATGGAAGAAAGACCCAACCTTTATTCCCAGCCTTACTCTTCTCCTTCTCCTACTGCCAATC TCCCTAGCCCTTTCCAAGGCATGGTCAGGCAAAAACCTTCACTGGGGACGATGCCTGTTCAAGTAACACC TCCCCGAGGTGCTTTTTCACCTGGCATGGGCATGCAGCCCAGGCAAACTCTAAACAGACCTCCGGCTGCA CCTAACCAGCTTCGACTTCAACTACAGCAGCGATTACAGGGACAACAGCAGTTGATACACCAAAATCGGC AAGCTATCTTAAACCAGTTTGCAGCAACTGCTCCTGTTGGCATCAATATGAGATCAGGCATGCAACAGCA AATTACACCTCAGCCACCCCTGAATGCTCAAATGTTGGCACAACGTCAGCGGGAACTGTACAGTCAACAG CACCGACAGAGGCAGCTAATACAGCAGCAAAGAGCCATGCTTATGAGGCAGCAAAGCTTTGGGAACAACC TCCCTCCCTCATCTGGACTACCAGTTCAAATGGGGAACCCCCGTCTTCCTCAGGGTGCTCCACAGCAATT CCCCTATCCACCAAACTATGGTACAAATCCAGGAACCCCACCTGCTTCTACCAGCCCGTTTTCACAACTA GCAGCAAATCCTGAAGCATCCTTGGCCAACCGCAACAGCATGGTGAGCAGAGGCATGACAGGAAACATAG GAGGACAGTTTGGCACTGGAATCAATCCTCAGATGCAGCAGAATGTCTTCCAGTATCCAGGAGCAGGAAT GGTTCCCCAAGGTGAGGCCAACTTTGCTCCATCTCTAAGCCCTGGGAGCTCCATGGTGCCGATGCCAATC CCTCCTCCTCAGAGTTCTCTTCTCCAGCAAACTCCACCTGCCTCCGGGTATCAGTCACCAGACATGAAGG CCTGGCAGCAAGGAGCGATAGGAAACAACAATGTGTTCAGTCAAGCTGTCCAGAACCAGCCCACGCCTGC ACAGCCAGGAGTATACAACAACATGAGCATCACCGTTTCCATGGCAGGTGGAAATACGAATGTTCAGAAC ATGAACCCAATGATGGCCCAGATGCAGATGAGCTCTTTGCAGATGCCAGGAATGAACACTGTGTGCCCTG AGCAGATAAATGATCCCGCACTGAGACACACAGGCCTCTACTGCAACCAGCTCTCATCCACTGACCTTCT CAAAACAGAAGCAGATGGAACCCAGGACAAGAAGACAGAAGAGTTCTTCTCTGTGGTGACTACAGACTAG >OriGene 5' read for NM_147223 unedited
GCACGAGGCCCATTCCCCGGGAAGCTACCCTCTGGAACTCAAGATTTGACCATATCTGTT TTGAGGATTCATTATGAACAAAGAAGTCTCCCAGGTGTGAAGTTTTTCAACATGAGTGGC CTCGGGGACAGTTCATCCGACCCTGCTAACCCAGACTCACATAAGAGGAAAGGATCGCCA TGTGACACACTGGCATCAAGCACGGAAAAGAGGCGCAGGGAGCAAGAAAATAAATATTTA GAAGAACTAGCTGAGTTACTGTCTGCCAACATTAGTGACATTGACAGCTTGAGTGTAAAA CCAGACAAATGCAAGATTTTGAAGAAAACAGTCGATCAGATACAGCTAATGAAGAGAATG GAACAAGAGAAATCAACAACTGATGACGATGTACAGAAATCAGACATCTCATCAAGTAGT CAAGGAGTGATAGAAAAGGAATCCTTGGGACCTCTTCTTTTGGAGGCTTTGGATGGATTT TTCTTTGTTGTGAACTGTGAAGGGAGAATTGTATTTGTGTCAGAGAATGTAACCAGCTAC TTAGGTTACAATCAGGAGGAATTAATGAATACCAGCGTCTACAGCATACTGCACGTGGGG GATCATGCAGAATTTGTGAAGAATCTGCTACCAAAATCACTAGTAAATGGAGTTCCTTGG CCTCAAGAGGCACACGACGAAATAGCCATACCTTTAACTGCAGG |
Restriction Sites | NotI-NotI |
ACCN | NM_147223 |
Insert Size | 4500 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_147223.2, NP_671756.1 |
RefSeq Size | 6952 bp |
RefSeq ORF | 4200 bp |
Locus ID | 8648 |
UniProt ID | Q15788 |
Protein Families | Druggable Genome, Transcription Factors |
Gene Summary | The protein encoded by this gene acts as a transcriptional coactivator for steroid and nuclear hormone receptors. It is a member of the p160/steroid receptor coactivator (SRC) family and like other family members has histone acetyltransferase activity and contains a nuclear localization signal, as well as bHLH and PAS domains. The product of this gene binds nuclear receptors directly and stimulates the transcriptional activities in a hormone-dependent fashion. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2), also known as SRC1e, contains an additional segment in the coding region compared to variant 1. The resulting isoform (2) contains a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Human Pregnane X Receptor Agonism by Ginkgo biloba Extract: Assessment of the Role of Individual Ginkgolides
,Aik Jiang Lau, Guixiang Yang, Ganesh Rajaraman, Christie C. Baucom, and Thomas K. H. Chang,
J. Pharmacol. Exp. Ther., Dec 2010; 335: 771 - 780
[NCOA1]
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Other Versions
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RC224865L4 | Lenti-ORF clone of NCOA1 (mGFP-tagged)-Human nuclear receptor coactivator 1 (NCOA1), transcript variant 2 |
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RG224865 | NCOA1 (tGFP-tagged) - Human nuclear receptor coactivator 1 (NCOA1), transcript variant 2 |
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