DCAMKL2 (DCLK2) (NM_152619) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DCK2, DCDC3, MGC45428, DKFZp761I032
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF sequence for NM_152619 edited ATGGCCAGCACCAGGAGTATCGAGCTGGAGCACTTTGAGGAACGGGACAAAAGGCCGCGG CCGGGGTCGCGGAGAGGGGCCCCCAGCTCCTCCGGGGGCAGCAGCAGCTCGGGCCCCAAG GGGAACGGGCTCATCCCCAGTCCGGCGCACAGTGCCCACTGCAGCTTCTACCGCACGCGG ACCCTGCAGGCCCTCAGCTCGGAGAAGAAGGCCAAGAAGGCGCGCTTCTACCGGAACGGG GACCGCTACTTCAAGGGCCTGGTGTTTGCCATCTCCAGCGACCGCTTCCGGTCCTTCGAT GCGCTCCTCATAGAGCTCACCCGCTCCCTGTCGGACAACGTGAACCTGCCCCAGGGTGTC CGCACTATCTACACCATCGACGGCAGCCGGAAGGTCACCAGCCTGGACGAGCTGCTGGAA GGTGAGAGTTACGTGTGTGCATCCAATGAACCATTTCGTAAAGTCGATTACACCAAAAAT ATTAATCCAAACTGGTCTGTGAACATCAAGGGTGGGACATCCCGAGCGCTGGCTGCTGCC TCCTCTGTGAAAAGTGAAGTAAAAGAAAGTAAAGATTTCATCAAACCCAAGTTAGTGACT GTGATTCGAAGTGGAGTGAAGCCTAGAAAAGCCGTGCGGATCCTTCTGAATAAAAAGACT GCTCATTCCTTTGAACAAGTCTTAACAGATATCACCGAAGCCATTAAACTAGACTCAGGA GTCGTCAAGAGGCTCTGCACCCTGGATGGAAAGCAGGTTACTTGTCTGCAAGACTTTTTT GGTGATGACGATGTTTTTATTGCATGTGGACCAGAAAAATTTCGTTATGCCCAAGATGAC TTTGTCCTGGATCATAGTGAATGTCGTGTCCTGAAGTCATCTTATTCTCGATCCTCAGCT GTTAAGTATTCTGGATCCAAAAGCCCTGGGCCCTCTCGACGCAGCAAATCACCAGCTTCA GTTAATGGAACTCCCAGCAGCCAACTTTCTACTCCTAAATCTACGAAATCCTCCAGTTCC TCTCCAACTAGTCCAGGAAGTTTCAGAGGATTAAAGATTTCTGCTCATGGCAGATCTTCT TCCAATGTAAACGGTGGACCTGAGCTTGACCGTTGCATAAGTCCTGAAGGTGTGAATGGA AACAGATGCTCTGAATCATCAACTCTTCTTGAGAAATACAAAATTGGAAAGGTCATTGGT GATGGCAATTTTGCAGTAGTCAAAGAGTGTATAGACAGGTCCACTGGAAAGGAGTTTGCC CTAAAGATTATAGACAAAGCCAAATGTTGTGGAAAGGAACACCTGATTGAGAATGAAGTG TCAATACTGCGCCGAGTGAAACATCCCAATATCATTATGCTGGTCGAGGAGATGGAAACA GCAACTGAGCTCTTTCTGGTGATGGAATTGGTCAAAGGTGGAGATCTCTTTGATGCAATT ACTTCGTCGACCAAGTACACTGAGAGAGATGGCAGTGCCATGGTGTACAACTTAGCCAAT GCCCTCAGGTATCTCCATGGCCTCAGCATCGTGCACAGAGACATCAAACCAGAGAATCTC TTGGTGTGTGAATATCCTGATGGAACCAAGTCTTTGAAACTGGGAGACTTTGGGCTTGCG ACTGTGGTAGAAGGCCCTTTATACACAGTCTGTGGCACACCCACTTATGTGGCTCCAGAA ATCATTGCTGAAACTGGCTATGGCCTGAAGGTGGACATTTGGGCAGCTGGTGTGATCACA TACATACTTCTCTGTGGATTCCCACCATTCCGAAGTGAGAACAATCTCCAGGAAGATCTC TTCGACCAGATCTTGGCTGGGAAGCTGGAGTTTCCGGCCCCCTACTGGGATAACATCACG GACTCTGCCAAGGAATTAATCAGTCAAATGCTTCAGGTAAATGTTGAAGCTCGGTGTACC GCGGGACAAATCCTGAGTCACCCCTGGGTGTCAGATGATGCCTCCCAGGAGAATAACATG CAAGCTGAGGTGACAGGTAAACTAAAACAGCACTTTAATAATGCGCTCCCCAAACAGAAC AGCACTACCACCGGGGTCTCCGTCATCATGTTTGATTTGACAGTTTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_152619 unedited ATTCGCACGAGGGCGCGTTAAGGGCCCTCGCAGTCAGACGTCCCTGCACCGGCGCTCGCA CCCTTAGTCGGCCCGGAACGTCTTTTTGCGGACGCCCTCGGAGCAGCCGCGATGGCCAGC ACCAGGAGTATCGAGCTGGAGCACTTTGAGGAACGGGACAAAAGGCCGCGGCCGGGGTCG CGGAGAGGGGCCCCCAGCTCCTCCGGGGGCAGCAGCAGCTCGGGCCCCAAGGGGAACGGG CTCATCCCCAGTCCGGCGCACAGTGCCCACTGCAGCTTCTACCGCACGCGGACCCTGCAG GCCCTCAGCTCGGAGAAGAAGGCCAAGAAGGCGCGCTTCTACCGGAACGGGGACCGCTAC TTCAAGGGCCTGGTGTTTGCCATCTCCAGCGACCGCTTCCGGTCCTTCGATGCGCTCCTC ATAGAGCTCACCCGCTCCCTGTCGGACAACGTGAACCTGCCCCAGGGTGTCCGCACTATC TACACCATCGACGGCAGCCGGAAGGTCACCAGCCTGGACGAGCTGCTGGAAGGTGAGAGT TACGTGTGTGCATCCAATGAACCATTTCGTAAAGTCGATTACACCAAAAATATTAATCCA AACTGGTCTGTGAACATCAAAGGTGGGACATCCCGAGCGCTGGCTGCTGCCTCCTCTGTG AAAAAGTGAGTAAAAGAAAGTAAAGATTTCATCAAACCCCAGTTAGTGACTGTGATTCGA AGTGGAGTGAAGCCTAAAAAGCCGTGCGGATCCCTCTGAATAAAAGACTGCTCATTCCTT TGAACAAAGTCTACAGATATCACCGAAACCTTTAACTAAACTCAGGAGTCGCCAAGAGCT CTGCACCCTGAAGGAAAGCAGGTACCTGTCTGCAAGACTTTTTTGAGAAGACGATGTT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_152619 unedited NAAAAATACTTGTTTCGNGAGGGCAAAGATTTATTGCCTAGGTTGGGGNACACTTGNTAA AAGGCAAAATCTAACAATGAGAACGAACTATAGGGCTCGCCAGTTTAGAAGATGAGCAGG TAAAACAAATACACGGATTATGTACAATTATTAAAAAGAGCAGGGTGGTGGACAAAAAGG AAAAACTGCCAGCAGCCTTCCAGAAGGTTCACAGAGTATATCCAGGGAGCACCTTCCCGC CCAGTCCTGGCTGCCTCGGGCATGCCTCCTCCTGTGGTTACCTGCCCTGCTCCAGATGAA ATGCAGATGAGGAAGCAGCTACCGGGGCTCAGGAGCAGAGCTCATTCTGCTCGGCAAGTC GCTGGCTTGCAGGGAAGCAGCCGCTGGGTGCCGCATGCGCCAAGCCTCTCATTAAAAAGC CCAGAGGGCAAGTTCACGGGCATTTCCTAACACACGTGTGGGAAGAGCTGGGGGAAACCC ACACCGCCCAGGGAAGAAGTGGCTTCTTCCATCGTCTGTGTATGAATGAATACTGAGCAT TGTCTCTGGGGAGCCTGGTGCCCCAAGCCGAATGGCTGAGCCCGCCGCCCTGGGCCTCTG AGCGCTTCTCAAAGCCTGCAATCCGTGTCCAATGACCAAATTGTCCTCTCAGGCGCTCAC TTCTTCTATTAGAAAGCCAGTCCTCTTAGCCCCCATATCACCTCTCACAGGACACCCACA AAAGGACAAATGCAATTCTAAGTCCTTCGTCAAAAAAAAGGAGAGAGAGAGAAAAGAAAA GAAAGGGAATGTTCTGGATTCTACTTTAATAAGGAAAAGGCCTTGGTTTCATCCAAAGTG TTTCCAAAGTGTTTTTATAAAAATGCCATCGATGGCTGGATAAAGTTGCCCATTCTCCTG GTGAAGCCGTAAAAGGCCG
Restriction Sites	NotI-NotI
ACCN	NM_152619
Insert Size	4700 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_152619.1, NP_689832.1
RefSeq Size	3626 bp
RefSeq ORF	2088 bp
Locus ID	166614
Domains	pkinase, TyrKc, S_TKc, DCX
Protein Families	Druggable Genome, Protein Kinase
Gene Summary	This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. Mouse studies show that the DCX gene, another family member, and this gene share function in the establishment of hippocampal organization and that their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Sep 2010]

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