Angiomotin like 1 (AMOTL1) (NM_130847) Human Untagged Clone
CAT#: SC100236
AMOTL1 (untagged)-Human angiomotin like 1 (AMOTL1)
CNY 10,512.00
Cited in 1 publication. |
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | JEAP |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC100236 sequence for NM_130847 edited (data generated by NextGen Sequencing)
ATGTGGAGGGCAAAGTTGCGCCGGGGAACTTGTGAGCCTGCGGTGAAAGGGTCCCCTTCT GCTTGTTATAGCCCCAGTAGTCCTGTCCAGGTTCTAGAAGACTCCACCTACTTTTCCCCA GACTTTCAGCTCTATTCTGGGAGGCATGAAACATCTGCTTTGACGGTGGAGGCAACCAGT AGCATCAGGGAAAAAGTTGTTGAAGATCCTCTTTGTAACTTCCACTCCCCAAACTTCCTG AGGATCTCAGAGGTGGAAATGAGAGGTTCCGAGGATGCGGCAGCTGGAACAGTATTGCAG CGGCTGATCCAGGAACAACTGCGGTATGGCACCCCAACCGAGAACATGAACTTGCTGGCC ATTCAGCACCAGGCCACAGGGAGTGCAGGACCAGCCCATCCTACAAACAACTTTTCTTCC ACGGAAAACCTCACTCAAGAAGACCCACAAATGGTCTACCAGTCAGCACGCCAAGAACCG CAGGGTCAAGAACACCAGGTGGACAATACGGTGATGGAGAAACAGGTCCGGTCCACGCAG CCTCAGCAGAACAACGAGGAACTGCCCACTTACGAGGAGGCCAAAGCACAGTCGCAGTTC TTCAGGGGGCAGCAGCAGCAGCAACAGCAGCAGGGGGCGGTGGGCCATGGTTACTACATG GCAGGGGGCACCAGTCAGAAGTCCCGAACTGAGGGGAGGCCCACTGTGAACCGTGCCAAC AGTGGACAGGCGCATAAGGACGAGGCGCTGAAGGAACTGAAGCAGGGCCACGTCCGCTCG CTCAGCGAGAGAATCATGCAGCTGTCCCTGGAGAGGAATGGGGCCAAGCAACACCTTCCC GGCTCGGGGAATGGAAAGGGCTTCAAAGTAGGAGGGGGGCCCTCCCCTGCCCAGCCTGCA GGTAAAGTGCTGGACCCTCGGGGTCCTCCACCTGAGTACCCCTTCAAGACCAAGCAAATG ATGTCCCCAGTCAGCAAGACCCAGGAGCACGGACTTTTTTATGGTGACCAGCACCCCGGG ATGCTCCACGAGATGGTCAAGCCCTACCCTGCTCCTCAGCCTGTGAGAACAGATGTGGCC GTCCTGCGGTACCAGCCACCCCCTGAGTATGGGGTAACGAGCCGCCCATGCCAACTTCCG TTCCCATCAACCATGCAGCAGCACAGCCCCATGTCCTCCCAGACCTCTTCCGCCAGCGGG CCACTGCACTCTGTCTCCCTGCCGCTTCCACTCCCGATGGCCCTGGGTGCTCCACAGCCC CCGCCTGCCGCCTCCCCCAGCCAGCAGCTTGGTCCAGATGCCTTTGCGATTGTGGAGCGA GCCCAGCAAATGGTGGAGATATTAACAGAGGAGAACCGGGTGCTTCACCAGGAACTTCAG GGTTACTACGACAATGCCGACAAGCTCCACAAGTTTGAAAAAGAACTTCAGAGAATTTCG GAAGCCTATGAAAGTCTGGTCAAGTCTACCACCAAGCGAGAATCGCTGGACAAGGCCATG AGAAACAAATTGGAAGGCGAGATTAGAAGACTTCATGATTTCAACAGAGACCTCCGAGAT CGACTAGAGACTGCTAACAGGCAACTATCCAGCAGGGAATACGAAGGGCATGAAGACAAA GCTGCAGAGGGGCATTATGCTTCCCAGAACAAAGAATTCTTGAAGGAAAAGGAGAAATTA GAAATGGAGTTAGCAGCAGTGCGGACTGCAAGTGAGGACCATCGGAGACACATCGAGATC CTGGACCAGGCTTTGAGCAACGCCCAGGCCAGGGTCATCAAGCTGGAAGAGGAGTTACGA GAGAAGCAAGCATATGTTGAGAAAGTTGAGAAGCTGCAGCAGGCCCTGACCCAGCTGCAG TCTGCATGTGAGAAGCGAGAACAGATGGAGCGGAGACTGCGGACTTGGCTGGAGAGAGAG CTGGATGCACTGAGAACCCAGCAGAAACATGGAAATGGCCAGCCAGCCAACATGCCGGAA TACAATGCCCCAGCCCTCCTGGAACTTGTGCGGGAGAAGGAGGAGCGGATCCTGGCCCTG GAGGCCGACATGACAAAGTGGGAGCAGAAGTACCTGGAGGAGAGCACCATCCGACACTTT GCCATGAATGCCGCAGCCACTGCAGCAGCTGAGAGGGACACCACGATCATCAACCACTCA CGGAATGGCAGCTACGGAGAGAGCTCGCTGGAGGCCCACATCTGGCAAGAGGAGGAGGAG GTGGTGCAGGCCAACAGAAGGTGTCAGGACATGGAATACACTATTAAAAATCTCCATGCC AAAATCATAGAGAAAGATGCTATGATTAAGGTCCTGCAGCAGCGATCTCGTAAAGATGCC GGGAAGACAGACTCCTCCAGCCTACGTCCTGCCCGCTCCGTTCCATCCATAGCAGCAGCT ACTGGGACACACTCTCGCCAGACCTCTCTTACCAGCAGCCAGCTGGCTGAGGAAAAGAAG GAAGAGAAGACCTGGAAGGGGAGCATAGGATTGCTGCTGGGGAAGGAGCACCATGAGCAT GCCTCTGCCCCACTGCTGCCACCCCCACCCACCTCAGCACTGTCCTCCATAGCCTCCACT ACGGCAGCCAGCAGTGCCCACGCCAAGACAGGCAGCAAGGACAGCAGCACACAGACTGAC AAGAGTGCCGAGCTCTTCTGGCCCAGCATGGCCTCCCTTCCCAGCCGCGGCCGGCTGAGC ACGACCCCTGCTCACAGCCCCGTCCTGAAACACCCAGCGGCCAAAGGGACCGCAGAGAAA CTGGAGAACTCTCCTGGCCATGGGAAGTCGCCTGACCACAGAGGCCGGGTCAGCAGCTTG CTGCACAAGCCCGAGTTCCCTGATGGAGAGATGATGGAAGTCCTCATCTAA Clone variation with respect to NM_130847.2 >OriGene 5' read for NM_130847 unedited
TCAACATTTGTAATACGACTCACTATAGGGCGGCCGCGCAGCGGTTGTCGGGTTTGGGGC TGGAGGTGAAGCCCTGTGTGAATGGGGTTGATTGTCCGGCGCCACTTCCCCGCGCTGCCC GGCAGCCGTCTTCCCCAGCCGAGGGACTGAACTAGCCATGATCGCCTCATGTGGAGGGCA AAGTTGCGCCGGGGAACTTGTGAGCCTGCGGTGAAAGGGTCCCCTTCTGCTTGTTATAGC CCCAGTAGTCCTGTCCAGGTTCTAGAAGACTCCACCTACTTTTCCCCAGACTTTCAGCTC TATTCTGGGAGGCATGAAACATCTGCTTTGACGGTGGAGGCAACCAGTAGCATCAGGGAA AAAGTTGTTGAAGATCCTCTTTGTAACTTCCACTCCCCAAACTTCCTGAGGATCTCAGAG GTGGAAATGAGAGGTTCCGAGGATGCGGCAGCTGGAACAGTATTGCAGCGGCTGATCCAG GAACAACTGCGGTATGGCACCCCAACCGAGAACATGAACTTGCTGGCCATTCAGCACCAG GCCACAGGGAGTGCAGGACCAGCCCATCCTACAAACAACTTTTCTTCCACGGAAAACCTC ACTCAAGAAGACCCACAAATGGTCTACCAGTCAGCACGCCAAGAACCGCAGGGTCAAGAA CACCAGGTTGGACAATACGGTGATGGAGAAACAGGTTCCGTCCACGCAGCCTCAGCAGAA CAACGAAGAACTGCCCACTTACGAGGAGGCCAAAGCACAGTCGCAGTTTTTCAAGGGGCA GCAGCATCACCACAGCAGCATGGGGCCGTGGGCCATGGTTACTACATGGCAGGGGGCACC ATTCAAAATTCCCGACCTGAGGGATGCCCCTGTGTACCGTGCCACCGGGACAAGCGCTTA AGACCAGCGCTTAAGAACTAATCAGGCCCTTCCGTTCGTCACGAGAGATATGCACTGCCC TGAGAGG >OriGene 3' read for NM_130847 unedited
NNNGGGTATAGCTATGNAACCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGATTCCCAGGGTGATCC CCATTTGGGTTTATCTGTTTATAAACAAAAAAAGGGGGGACCCCAGCCCCCCCCAAAAAA ACATTTTTTGGACAAAGAAAAAATCAATTTCCCATTTTTTAAATTTGTTTGGAAAACTAC ACCCCTCTTTTTTTTTTCACCATGTGTAAAAAAAACCCTCGCAATTTCTTTTATCTTCAA TCATGGAGTTTTTGGGGTTAAAAATTATAAAAAGGGTTCTTATGAATCTGAAAACTTTAT CAATTCAAAACATTCCTGAATTCCCATCTACAACCCTTTAAGGCTTTTTTCTTTTTCTGC CGCTTTCCTTGGTTGGCAATGTTCTGGACTGAAATTCCCCAAGGGTGGGCGTTAAATGAA GACTTTCCTCATCTCTCCCTCAAGGAACTCGGGCTTGTGCAACAAGCTGCTGACCCCGCC CCTGTGGTCAAGCGAATTTCCCTGGGCCGGAAAATTTTCCAGTTTTTCTGCGGGCCCTTT TGCCCCTGGGTGTTTTAAGACCGGGCTTTGAACAAGGGTTCTGCTCAACCCGCCGCGGCT TGGAAAGGAAGCCCTGCTTGGCCAAAAAAACTCGGCACCCTTGTCAATCCGTGTGCCGCT GGCCCTGCTGCCCGTCTTGGCGTGGGCACTGGTGGCTGCCCTAATGGAAGCCATTGAAGA CAATGCCTTAGTGGGTTGGGGGGGGGCCAACCATGGGGGCACAAGGCATTCCTCTGGGTG GTTTCCTTTCCCCAGAAACCATCCTATAGCTCCCTTTCAGGTTTTTTCCTTCCTTTTTTC |
Restriction Sites | NotI-NotI |
ACCN | NM_130847 |
Insert Size | 4000 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_130847.1, NP_570899.1 |
RefSeq Size | 5299 bp |
RefSeq ORF | 2871 bp |
Locus ID | 154810 |
UniProt ID | Q8IY63 |
Protein Pathways | Tight junction |
Gene Summary | The protein encoded by this gene is a peripheral membrane protein that is a component of tight junctions or TJs. TJs form an apical junctional structure and act to control paracellular permeability and maintain cell polarity. This protein is related to angiomotin, an angiostatin binding protein that regulates endothelial cell migration and capillary formation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Hippo Pathway-independent Restriction of TAZ and YAP by Angiomotin
,Siew Wee Chan, Chun Jye Lim, Yaan Fun Chong, Ajaybabu V. Pobbati, Caixia Huang, and Wanjin Hong,
J. Biol. Chem., Mar 2011; 286: 7018 - 7026
[AmotL1]
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207693 | AMOTL1 (Myc-DDK-tagged)-Human angiomotin like 1 (AMOTL1) |
CNY 10,504.00 |
|
RC207693L1 | Lenti-ORF clone of AMOTL1 (Myc-DDK-tagged)-Human angiomotin like 1 (AMOTL1) |
CNY 12,904.00 |
|
RC207693L2 | Lenti-ORF clone of AMOTL1 (mGFP-tagged)-Human angiomotin like 1 (AMOTL1) |
CNY 12,904.00 |
|
RC207693L3 | Lenti-ORF clone of AMOTL1 (Myc-DDK-tagged)-Human angiomotin like 1 (AMOTL1) |
CNY 5,890.00 |
|
RC207693L4 | Lenti-ORF clone of AMOTL1 (mGFP-tagged)-Human angiomotin like 1 (AMOTL1) |
CNY 5,890.00 |
|
RG207693 | AMOTL1 (tGFP-tagged) - Human angiomotin like 1 (AMOTL1) |
CNY 12,104.00 |
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