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MARCHF8 (NM_145021) Human Untagged Clone
CAT＃: SC100050
41341 (untagged)-Human membrane-associated ring finger (C3HC4) 8 (MARCH8), transcript variant 8
CNY 2,400.00
CNY 2,950.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | c-MIR; CMIR; MARCH-VIII; MARCH8; MIR; RNF178 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_145021, the custom clone sequence may differ by one or more nucleotides
ATGAGCATGCCACTGCATCAGATCTCTGCCATTCCATCCCAGGATGCCATCTCTGCTAGAGTCTACAGAA GTAAGACCAAAGAAAAGGAGAGGGAAGAACAGAATGAGAAGACTTTGGGACATTTCATGAGTCATTCAAG CAACATTTCTAAGGCTGGGAGTCCTCCGTCAGCATCAGCTCCGGCTCCGGTGTCCTCCTTCTCTCGCACT TCTATCACGCCATCCAGCCAGGACATCTGCAGGATCTGCCACTGTGAAGGAGATGATGAGAGCCCCCTGA TCACCCCCTGCCACTGCACAGGAAGCCTCCACTTCGTGCACCAGGCCTGCCTGCAGCAGTGGATCAAGAG CTCCGACACGCGCTGCTGCGAGCTCTGCAAGTATGAGTTCATCATGGAGACCAAGCTGAAGCCACTGAGA AAATGGGAGAAGTTGCAGATGACGTCCAGCGAGCGCAGGAAGATCATGTGCTCAGTGACATTCCACGTCA TTGCCATCACATGTGTGGTCTGGTCCTTGTATGTGCTCATTGACCGTACTGCTGAGGAGATCAAGCAGGG GCAGGCAACAGGAATCCTAGAATGGCCCTTTTGGACTAAATTGGTGGTTGTGGCCATCGGCTTCACCGGA GGACTTCTTTTTATGTATGTTCAGTGTAAAGTGTATGTGCAATTGTGGAAGAGACTCAAGGCCTATAATA GAGTGATCTATGTTCAAAACTGTCCAGAAACAAGCAAAAAGAATATTTTTGAAAAATCTCCACTAACAGA GCCCAACTTTGAAAATAAACATGGATATGGAATCTGTCATTCCGACACAAACTCTTCTTGTTGCACAGAG CCTGAAGACACTGGAGCAGAAATCATTCACGTCTGA >OriGene 5' read for NM_145021 unedited
NGGGTTATTACCCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTA TATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGC GGCCGCGAATTCGGCACGAGGGTTGGGATGAGCATGCCACTGCATCAGATCTCTGCCATT CCATCCCAGGATGCCATCTCTGCTAGAGTCTACAGAAGTAAGACCAAAGAAAAGGAGAGG GAAGAACAGAATGAGAAGACTTTGGGACATTTCATGAGTCATTCAAGCAACATTTCTAAG GCTGGGAGTCCTCCGTCAGCATCAGCTCCGGCTCCGGTGTCCTCCTTCTCTCGCACTTCT ATCACGCCATCCAGCCAGGACATCTGCAGGATCTGCCACTGTGAAGGAGATGATGAGAGC CCCCTGATCACCCCCTGCCACTGCACAGGAAGCCTCCACTTCGTGCACCAGGCCTGCCTG CAGCAGTGGATCAAGAGCTCCGACACGCGCTGCTGCGAGCTCTGCAAGTATGAGTTCATC ATGGAGACCAAGCTGAAGCCACTGAGAAAATGGGAGAAGTTGCAGATGACGTCCAGCGAG CGCAGGAAGATCATGTGCTCAGTGACATTCCACGTCATTGCCATCACATGTGTGGTCTGG TCCTTGTATGTGCTCATTGACCGTACTGCTGAGGAGATCAAGCAGGGGCAGGCAACAGGA ATCCTATAAGGGCCCTTTGGACAAATTGGTGGGTGTGGCCATCGGCTTCACCGGAGGACT TCTTTTTATGTATGTTCACTGCAAAGCGCATGTGCCATTGTCGGAAAAGACCCAGGCCCT TTATAGAAAGGACTATTGTTCAAAACCGTCCCGAAACAACCAAAAAGAATATTTTTCGAA AAACTCCACCAT >OriGene 3' read for NM_145021 unedited
TCNAACGTAATCTATGNACGCGGCCGCATTCTANGATCGGTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTTTAAAAAAAGGCTTTATT GGTGCAATTAAACATCCCAGGGTATAGCACAAACCCCCCTGTACAAAAAATTATTCAAGG GGTAATACTGAAAAACAGTGAACCCCCCCAAAAAAATACAAAACTAGCCATTTAGATCAC TAAAAACTTTTTAAGGGAAAAAAAATTTCAAATGGGAAGGGCCTTTTAAAAACTACCCCC CACATGCCAGGGGAAATTTGGTTTAACAATCAATTTTTATAAACATTGGATTTAACTGGG CACTGATAAACTGGACAATTTTAAAAATTACTTCCAAGACAAAAATAAATGCTTTTATTT CCCTATGTTTTGGGCTTTCCTTGGGCTAAAAACAAAAGCTGCATGTTTCAAACCCAATTT CAAAGGGAAAGCCCTTTAAAACAAAAAGGACTTCAATTGGGTTTTTGCCTTTAGCCACAT CCAAAGACTTGGGGATCTCACCCGGGTGGTCCCAACCCTTTTTCAACCCTGAAACCCAAG CAGGGGGGCAAAGGCCCCCCCCAATTGTGCAAACATTCACCGAGGTTCCAAAATGGCCCA ACTGGCTTGGGCCTGTTTGCCTGTGGGCCAGTTAGGAACAATGTGCCGGTTTGAAAAACA AGGCCCCCCAAACTGGAAAAGTGGCGCGAAACCACAAGGGGGGAAAAAATTTGAGGCGCT CACGGGGGGAAAGAACCCTTTTATAGAGTTTTCCGGAAAATCTTACCAAAAAATTGGGCC ACAAAACAAATTTTTGGAGAGCAAAAACCCTCTTTATGGTACCGGGGGATT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_145021 |
| Insert Size | 4700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_145021.4, NP_659458.2 |
| RefSeq Size | 2511 bp |
| RefSeq ORF | 876 bp |
| Locus ID | 220972 |
| UniProt ID | Q5T0T0 |
| Domains | RING |
| Protein Families | Druggable Genome, Transmembrane |
| Gene Summary | MARCH8 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH8 induces the internalization of several membrane glycoproteins (Goto et al., 2003 [PubMed 12582153]; Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Apr 2010] Transcript Variant: This variant (2) differs in the 5' UTR and lacks an in-frame exon in the 3' coding region, compared to variant 4. This results in a shorter protein (isoform a), compared to isoform b. Variants 2 and 3 encode the same isoform (a). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The E3 ubiquitin ligase MARCH8 negatively regulates IL-1ß-induced NF-B activation by targeting the IL1RAP coreceptor for ubiquitination and degradation
,Rui Chen, Mi Li, Yu Zhang, Qian Zhou, and Hong-Bing Shu,
PNAS, Aug 2012; 109: 14128 - 14133.
[MARCHF8]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209891 | MARCH8 (Myc-DDK-tagged)-Human membrane-associated ring finger (C3HC4) 8 (MARCH8), transcript variant 8 |
CNY 2,400.00 |
|
| RC209891L1 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 8 (MARCH8), transcript variant 8, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209891L2 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 8 (MARCH8), transcript variant 8, mGFP tagged |
CNY 4,800.00 |
|
| RC209891L3 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 8 (MARCH8), transcript variant 8, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209891L4 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 8 (MARCH8), transcript variant 8, mGFP tagged |
CNY 5,890.00 |
|
| RG209891 | 41341 (tGFP-tagged) - Human membrane-associated ring finger (C3HC4) 8 (MARCH8), transcript variant 8 |
CNY 4,000.00 |
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