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PDE5A (NM_033437) Human Untagged Clone
CAT＃: SC100003
PDE5A (untagged)-Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 3
CN¥ 6,032.00
货期*
现货
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CGB-PDE; CN5A; PDE5 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_033437, the custom clone sequence may differ by one or more nucleotides
ATGGTCAATGCATGGTTTGCTGAGAGAGTTCACACCATCCCTGTGTGCAAGGAAGGTATCAGAGGCCACA CCGAATCTTGCTCTTGTCCCTTGCAGCAGAGTCCTCGTGCAGATAACAGTGCCCCTGGAACACCAACCAG GAAAATCTCTGCCTCTGAATTTGACCGGCCTCTTAGACCCATTGTTGTCAAGGATTCTGAGGGAACTGTG AGCTTCCTCTCTGACTCAGAAAAGAAGGAACAGATGCCTCTAACCCCTCCAAGGTTTGATCATGATGAAG GGGACCAGTGCTCAAGACTCTTGGAATTAGTGAAGGATATTTCTAGTCATTTGGATGTCACAGCCTTATG TCACAAAATTTTCTTGCATATCCATGGACTGATATCTGCTGACCGCTATTCCCTGTTCCTTGTCTGTGAA GACAGCTCCAATGACAAGTTTCTTATCAGCCGCCTCTTTGATGTTGCTGAAGGTTCAACACTGGAAGAAG TTTCAAATAACTGTATCCGCTTAGAATGGAACAAAGGCATTGTGGGACATGTGGCAGCGCTTGGTGAGCC CTTGAACATCAAAGATGCATATGAGGATCCTCGGTTCAATGCAGAAGTTGACCAAATTACAGGCTACAAG ACACAAAGCATTCTTTGTATGCCAATTAAGAATCATAGGGAAGAGGTTGTTGGTGTAGCCCAGGCCATCA ACAAGAAATCAGGAAACGGTGGGACATTTACTGAAAAAGATGAAAAGGACTTTGCTGCTTATTTGGCATT TTGTGGTATTGTTCTTCATAATGCTCAGCTCTATGAGACTTCACTGCTGGAGAACAAGAGAAATCAGGTG CTGCTTGACCTTGCTAGTTTAATTTTTGAAGAACAACAATCATTAGAAGTAATTTTGAAGAAAATAGCTG CCACTATTATCTCTTTCATGCAAGTGCAGAAATGCACCATTTTCATAGTGGATGAAGATTGCTCCGATTC TTTTTCTAGTGTGTTTCACATGGAGTGTGAGGAATTAGAAAAATCATCTGATACATTAACAAGGGAACAT GATGCAAACAAAATCAATTACATGTATGCTCAGTATGTCAAAAATACTATGGAACCACTTAATATCCCAG ATGTCAGTAAGGATAAAAGATTTCCCTGGACAACTGAAAATACAGGAAATGTAAACCAGCAGTGCATTAG AAGTTTGCTTTGTACACCTATAAAAAATGGAAAGAAGAATAAAGTTATAGGGGTTTGCCAACTTGTTAAT AAGATGGAGGAGAATACTGGCAAGGTTAAGCCTTTCAACCGAAATGACGAACAGTTTCTGGAAGCTTTTG TCATCTTTTGTGGCTTGGGGATCCAGAACACGCAGATGTATGAAGCAGTGGAGAGAGCCATGGCCAAGCA AATGGTCACATTGGAGGTTCTGTCGTATCATGCTTCAGCAGCAGAGGAAGAAACAAGAGAGCTACAGTCG TTAGCGGCTGCTGTGGTGCCATCTGCCCAGACCCTTAAAATTACTGACTTTAGCTTCAGTGACTTTGAGC TGTCTGATCTGGAAACAGCACTGTGTACAATTCGGATGTTTACTGACCTCAACCTTGTGCAGAACTTCCA GATGAAACATGAGGTTCTTTGCAGATGGATTTTAAGTGTTAAGAAGAATTATCGGAAGAATGTTGCCTAT CATAATTGGAGACATGCCTTTAATACAGCTCAGTGCATGTTTGCTGCTCTAAAAGCAGGCAAAATTCAGA ACAAGCTGACTGACCTGGAGATACTTGCATTGCTGATTGCTGCACTAAGCCACGATTTGGATCACCGTGG TGTGAATAACTCTTACATACAGCGAAGTGAACATCCACTTGCCCAGCTTTACTGCCATTCAATCATGGAA CACCATCATTTTGACCAGTGCCTGATGATTCTTAATAGTCCAGGCAATCAGATTCTCAGTGGCCTCTCCA TTGAAGAATATAAGACCACGTTGAAAATAATCAAGCAAGCTATTTTAGCTACAGACCTAGCACTGTACAT TAAGAGGCGAGGAGAATTTTTTGAACTTATAAGAAAAAATCAATTCAATTTGGAAGATCCTCATCAAAAG GAGTTGTTTTTGGCAATGCTGATGACAGCTTGTGATCTTTCTGCAATTACAAAACCCTGGCCTATTCAAC AACGGATAGCAGAACTTGTAGCAACTGAATTTTTTGATCAAGGAGACAGAGAGAGAAAAGAACTCAACAT AGAACCCACTGATCTAATGAACAGGGAGAAGAAAAACAAAATCCCAAGTATGCAAGTTGGGTTCATAGAT GCCATCTGCTTGCAACTGTATGAGGCCCTGACCCACGTGTCAGAGGACTGTTTCCCTTTGCTAGATGGCT GCAGAAAGAACAGGCAGAAATGGCAGGCCCTTGCAGAACAGCAGGAGAAGATGCTGATTAATGGGGAAAG CGGCCAGGCCAAGCGGAACTGA >OriGene 5' read for NM_033437 unedited
GTATGATTATGTAATACGATTTACTATAGGGCGGCCGCGAAATTCGCACCAGGCGGGGAA GCTGGGGTGAGGGACGAGTCCACGGGACAGCCCAAAGGCAACATGACGGAACCTTGCCAA AAGAAATGGTCAATGCATGGTTTGCTGAGAGAGTTCACACCATCCCTGTGTGCAAGGAAG GTATCAGAGGCCACACCGAATCTTGCTCTTGTCCCTTGCAGCAGAGTCCTCGTGCAGATA ACAGTGTCCCTGGAACACCAACCAGGAAAATCTCTGCCTCTGAATTTGACCGGCCTCTTA GACCCATTGTTGTCAAGGATTCTGAGGGAACTGTGAGCTTCCTCTCTGACTCAGAAAAGA AGGAACAGATGCCTCTAACCCCTCCAAGGTTTGATCATGATGAAGGGGACCAGTGCTCAA GACTCTTGGAATTAGTGAAGGATATTTCTAGTCATTTGGATGTCACAGCCTTATGTCACA AAATTTTCTTGCATATCCATGGACTGATATCTGCTGACCGCTATTCCCTGTTCCTTGTCT GTGAAGACAGCTCCAATGACAAGTTTCTTATCAGCCGCCTCTTTGATGTTGCTGAAGGTT CAACACTGGAAGAAGTTTCAAATAACTGTATCCGCTTAGAATGGAACAAAGGCATTGTGG GACATGTGGCAGCGCTTGGTGAGCCCTTGAACATCAAAGATGCATATGANGATCCTCGGT TCAATGCAGAAGTTGACCAAATTACAGGCTACAAGACACAAAGCATTCTTTGTATGCCAA TTAAGAATCATANGGNNAAGAGTNGTTGGTGTAGCCCAGGCCATCAACAAGAAATCAGNA AAACGTGGGACATTTACTGAANAAAGATGAAAAGACTNTGCTNGCTATTTGGCATTNTGN GGNATTNGTCTTATAATGCTCAGCTCTATGAGACTTACTGCTGNAGAACAAGAGAATANG TGCTGNCTGACTTGCTAT >OriGene 3' read for NM_033437 unedited
AACTGTGNACCGCGGCCCGCTATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTGCTTCT ATTTTTTGTTTTTTTACCAGAATGATAAAAATAAAACTGCATTATGTCAAGATATTTGTA CACACTCAGAATTTTAAAAACTTAAGGAACATACTGAATTTTAGGCAAAATTTAGGGGAA TCGGGACATAGATGGGAAAACACATTTTGGCGAAAGGTCCTTGAGAATGCTGGATTACCA TGTCCCTTAGAAATTTTCTATCCAAATTAGTGATAGAAACTCAGATCAAATATCTTTTTT GTGGGTTGGAGGTAGAAGTTAACAAATCAAGCAAATCTCTCCTTCTACTCTCCCCAAATC CAAACATTCCTGCATATTTGAAACAAATTTTCCTTTCCTAGACCAGTTGCTTAATTCATT ATCTACTTCTCCAATATTGAGTAAATGTGTCTTTAGGGGCACACATGTAGAATATTAATC CACTTAAATGGGCTCAAATTCTAAGAAAATATTAACTATACTAAATGTTATGGGAAGAAA ATACATTGAATGTGAGTTAAGGGCCAGTGTTTAGCATTCACATCCAGGATTCTCTGGATC TCAGTTGACTTGTCCTATACATCAAAATATGGCAATTTTTCAATCACAATACTGCTAAAT GTTATTCAGGCATAAAGTTTCTTACCCACTGTTCCCTCAAATTCATTTTTGACCCTAGTA TTGGCAATAGCCCTTTGCTATTTATATAATTAAAACTTTNTCTTTAATTTCAATTGACAG GAAAAAGAGTCATTTTGTACTCTCTTATCTATACCATGTCTCACTTCAGTCAATGCTACA TTGTCACTTTTATTTTGAAAAGGAGGCTCAGAGAAAAAANCTCTAGAGAGATCTCTACAG ACATTTTNCTTCCTGATAGACATCCAGCCATTCATTCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_033437 |
| Insert Size | 4700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_033437.2, NP_246273.2 |
| RefSeq Size | 6788 bp |
| RefSeq ORF | 2472 bp |
| Locus ID | 8654 |
| UniProt ID | O76074 |
| Domains | PDEase, GAF |
| Protein Families | Druggable Genome |
| Protein Pathways | Progesterone-mediated oocyte maturation, Purine metabolism |
| Gene Summary | This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream start codon, compared to variant 1. The encoded isoform (3, also known as PDE5A3) is shorter at the N-terminus, compared to isoform 1. There are no publicly available full-length transcripts supporting this variant; it is represented based on full-length cloning by RT-PCR in PMID:10679255. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216759 | PDE5A (Myc-DDK-tagged)-Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 3 |
CN¥ 8,170.00 |
|
| RC216759L3 | Lenti-ORF clone of PDE5A (Myc-DDK-tagged)-Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 3 |
CN¥ 10,070.00 |
|
| RC216759L4 | Lenti-ORF clone of PDE5A (mGFP-tagged)-Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 3 |
CN¥ 10,070.00 |
|
| RG216759 | PDE5A (tGFP-tagged) - Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 3 |
CN¥ 9,030.00 |
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