Rp1 (NM_001195676) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1560182; Rp1h
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217744 representing NM_001195676 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGAGACACCTTCTACTAGTTTCTCCATGATTCGTCTGACTTCTTCTGAAGGTCAAGTTCCTTCTC GCCATTCAAATGTCACTCATCCCACAGTGGCTAAACGCATCAGTTTCTATAAGAGCGGAGATCCCCAGTT TGGTGGTGTTAGGGTGGTGGTCAACCCTCGTTCCTTTAAGACTTTTGATGCCCTGCTAGACAATTTATCC AGGAAAGTGCCCTTGCCCTTTGGGGTGAGGAATATCAGCACGCCCCGTGGACGGCACAGCATCACCAAGC TAGAGGAGCTAGAGGACGGCGAGTCTTATGTATGCTCCCACAATAAGAAGGTGCTTCCGGTTGACCTGGA CAAGGCCCGCAGGCGCCCTCGGCCCTGGCTCAGTAGTCGCTCTATAAGCACACATGTGAAGCTCCGCTCT GCTACTGCCACTATGCCCACCACCGCTCCTGGCTTGTTTCGTGCCCCAAGGAGGCTTGTGGTCTTTCGGA ACGGTGACCCAAAGACCAGGCGTGTGGTCCTTCTTAGCCGGAGGATCACTCAGAGCTTCGAAGCCTTTCT ACAGTACCTGACACAGGTCATGCAGTATCCTGTGGCAAAGCTGTATGCCACAGACGGAAGAAAAGTTCCT AGTCTCCAAGCAGTGATACTGAGCTCTGGAGCTGTGGTGGCAGCCGGAAGGGAGCCATTCAAACCAGGAA ATTACGACATCCAAAAATACTTGCTTCCTGTCAAGTTACCAGGAATCTCTCATCGCGTGCACCGGAAGGG AAAAGCCAAGATAGAAAAAAGAAAGATGAGCACACATTCAGTCCCAAGACCCCAGACTGATTCCCTGGCC TCAGAGAAAACATATGATTGCTTTTCAGACTTTTCTGTAGCCCCTGAGAATTACCTGGCCTTAGAGACAC ATGATTCTCAGACTCTATCAACATATCCTTCTGAAGATGGTGTTGAGAAATCGATAGTTTTTAATCAAGA TGGTACTATGACGGTCGTGATGAAAGTTCGATTCAAGATAAAAGAAGAAACTGTTAAGTGGACAACCACT GTCAATAGAGCTGATCTTTCTAATGATGATGAAAAGAGTAAGATAAGTAGCTATCCTGGTAAAACAGAAG ATCGATCGTCTAGTTTAAAGCTTGTGGTGCCATGCTCATTGTCTGAAGACATCACAGACACCACTCAGCA GGGCAGTTTGACAGAGGAAGAAAATACTCAAATGACAGAACAGCAGGCTCTGTCAAGTAGCTCTGCTGGC TGGGAGAATGCCTCCATGGAGGTAGGCATTCAGGGAAGCCAGAAACAAGTGAAACACTTTTATAGACCCC CAACCCCTGGACCAAGGAGAATGAGACAAAAGAAATCTGTGATAGGGACTGTCACCGTAGTATCTGAAAC AGAGGTTCAAGAAAAGCAGTTTTCTTATAGGGAAGAAAGAGAGGTTGGAGAAAAATCCAAGTACCACATG TTTACACATTCTTGCAGCAAAATGTCCTCAGTATCTAATAAACTCGTTCAGATCAGTAGCGATGATGAGA GGGAGTCAGCATTAGAAAGAACAAAGGAAAGTGGACCGCTCAAGTCACATGCCATAAATGCTGGTGCTAT AGAAATTACAAGTCAGAGGGTCTTAAAGATGTGCCATAACAATGCTTTAGCGTCAACTGTACCAGAGAAC TCAGTTGTGGAAGAAGGCACAGATGACAGTGCCATATCAGGCAAAGCCACTATCAAGCACTTCAGAACTT GTGGTAATGCCAATGACAGCTTCAGTTCTACTACAGCAGATACAACTCTTACCTCAGGTAACAACTGTCC AAATGTCAGGACTATTTCTGAGGTTCCTTCTGTAGGATCTCCTGTGCTCACCACAGGACTTGTTAATGAA TTTGCTCATTGTGGTTTAACAGAACATCCAGGAAATGGAAGGCAGATTTCATCCCCTTTTGCCAGCAAAA AAAAGAAGAAATCTCAGCAGCGAATGATAACTTCCAAGGATAAGAAAAAAGTGATTGAAACCAAAGGAAT CCCTAACAAAGTTGGGAAAATACACAGAGCAGGAACAATTGCACAGGTGGTACTATTGCAAGATTCGGGT GGTCTGGATAAAGAAGGGAGAGTTCGTGAGGAGGAACTTGACACAAGTCCTATGGTAATTGAATCACATG ATATTTTCCCCAAAGGTAATTCCAAATTTTCCAAGAATTTCCATAAAAACAAATTAAATACTGTTCAAAA TCCTAAGACTCAAAAACTTTTAGCTAAAAGAAAATCAAGACCACTAAAGATTAAAAGCATAGGTGAACTC AGAAAACAAGAGACTGGTCAAGAAGATAAAGTATTTCTCCATAGCGAATCTAAACTTTGTGAAAGTAATT TGGAAAAACAAAGTTTACTTCATGTGTTTAGTAGTCTTGAGGAAGACCAGAAAGCTTTGCATAGATCACA GTCACAAGTGGAGACAGTAACTGAAAATTGGAAAGGAATGACAAAAAAGAGTTTAGTCCCAAAAGTTAAT GATCTACACGTAACTGTAAGAAACCAGAGAAAACAAAAGGGAGTTAAATTAAAGTCAGGTGCTACAGTAA GTGAACAGCATATTGCAACCAGGGCGGATCCCTTAGCATCTTTGAAAAAACCTGATTTAACCGAGGGTAT TCCCCATCACTCAGTTAAGAGCTATATACAGCGATGGTTGCAGAACATAAATCCATATCCAGATTTTGAG CACAGAAAGTCAGCTCCACTAGGCCAAGATAGAAGTAATGTGGTAAATTATAACAGTAATGGTTTTCCGG AAAATAATCTGCCTACAACTTCTTCAAAAGGAAAGAGTTTTTTAATGGAAAGTAATAAGCGCAAAACTAA AAATGCCAATTGGAAAGGCAATAAAAATCAAGAGACAAGTAAATCTCTTGTTGTTAAAGATAATGGTGAA CAGCCTAATAAACATTCCTGTGAGAGCCAAGATGAGTCTCTGTATGATTCTTACTTGGGTCCTTTGCATG ACAATTGTACTTTGTCACAGACCAGTATAAACGATCCTTGTACTAAAAGTCATTTATCTACTGAAAAGTC AAGACCAGAAGTGAAGCTTGTTTACCAAGAAATGAACTTTTCCACAAAAAGGCAAACTCTTGAGGTTGCC ATTCAGGTAGACACTATGGGAGAGAATGTTCCAAAAGACTTACTAGCTCTGTTGCTCCAGCACCTGGAAG CTTTTATTCCTAATAATCAGAAGCATCAAAATGGAATTTCTCAACTACCAGGTTCATTAGCAGAGGTTGT CTTCCCTTCTGCAATATATAATTCATCCACAAATCTCCTTCTGGCTTGGCTTTTGGTACTAAACCTAAAG AGAAATATGAATAGCTTCTGTCAAAGCGATGCTCCTAAGACGACCAGCAGATCTTCAGAAACGGCTGCAC TGTTGGATGTTCTGAAGCACGTCGCCATCACAGAGGAAGCCGATGACTTGAAGGCCGCTGTTGCCAACTT AGTGGAATCAACCAAAACTTGCAGTGGACCCAGCGGGAGAGAACAAGATACGGCACCAGCAAATTGTATA GCAGCCAGCCTTCACAGTGTGGACAAGTGTAAGGAAAACAAGAGTACCCAGAAGAGCCTATTGGATGAAG GCTACTCTGTCAGGGGGGACTGTGGCCCCGAGCTCTGTGTTTTAGAAATGGTTAGCAAGTCAAACAACAC TCCAAGTGAGGGGCATACTGTAAGCATGACTTATCCTCCAAAAGGGGTAAATAACCAAAATGATGCCCTT TTCACTAGTAATGATAGTTCTACTATGAGCCAGCGTTCCACACACAATGCTTCTTTCCTAGGAGAGGCCT GTTTACTTACAGATAGTTTGTGTTCCCATAAGGCTTGTGCCCAGAAGGACAACATCTATGAGGCAGCTTG CTTGAGTGATGAGACTCACATTCCCATCAGAGACTGCAAAACAATTGAGTTCGTGAATTCAAAAGAAAAC AAATGCACTGACAATCTGGAATTAACTGAAGAAATCAAAACTGTTGACAAAGTTCCAAAAGACCTAAATA TTTTGGCAGATTCTATAGATAAAAATGAATCTGATATATCAACTTCATCCCAAAATGTCAATAAGTTAAG TTCCCAAGGTATTTTCCTAAGCAAAACTCATCTAGATTCTGACAAGGACTATAGTCCTCTAGAAGAATTT CAAAATTGCCCACCTAAGAAAATTGTGAATAAAAAGAAATCTGTTTCTTCTGATAAGGAAGATTCAAGGA CTTCTGAAGAACCAAGGTCAATAACCAATAGTGTGACATCAAGTGAAAGAAATGCCACTTCAGAATTAGA ATCTTTTGAAGAATCAGAAAGTCAAGACACAGGTATCTTCAATATGAAGGTAAGGACTGAAGAAAAAGCT GCCAAGGAGTCAATGCAAAAAGAATCAGAAGCTAGGATGAGTTTGGAATTGATAAACATATCAGGGAGGA ATATTATAGAACAGGGAAGAAGAAATACTGCATTTCTGGAGACAACAGGTAGGGGGCAGGTGACACCACC ATCTTTAGTTTTTTGCTATGATTCTAATAAAAACACTGAAAAAGAGATGAGTGTAGGAGAAACTAAGATG AGAGTTAAGAAGATAGTAGACAGCATGGAAAATGAAAGTTACACAGACTCTTCACTTAATTGTAACAAAC AGCACAGAAGTCCAGGGACCTTAGATTGGTCAGATTATGGATCAGACAGTGAGAGCCGATATCCTTGTAA AGCATTGAGCTATGAGCACAGTGATGACACCGGCCAAGAGAGAGAGTACCCTAGAGGAATTGTGAGAAGG GCAATAGAAAAGCTTTATGGCAAAGCAGAGATTATCAAACCACCATTTTTCCATGGGTCTATACACAAAT CTCAAGTTTGTCCTTATAATCCTGTGGAAGTTCCGTGTGTTAAGAAAACACATTTTTATGAGTCTGAATG TCAGTCACTGGTCTCTTCTGAACAGGTATCTAGAAGTTCACTCATGTTGCAGGAATTCCAGGAGGAAAGA CAATCTGAGGTTGATGCCAATGGCATGGGAGACAGTTTTGGGGACAGCAGCATAGAAAAGGTTACAAAAC CAAGTGAGCATGGCAGGGTCTTTATGGAAAAAGAGAAGGGAAAACTGATTGACAATGGCAAATGGCTCCT GAGAGAAAATCATTTGTGGAGGGTGTCACTGGACAATCCTGGCATGTATGGCAATGCAGACACCACATCA GTGGATACACTAATGGATAAGAATAGCATTGAGGTGCCATATTCACATTTCGGAGAGTTGGCTCCTGGTC CAACAATGGCTGAATTGTCCTCTTCAGAAATAGAGGAAATGACTCAACCCCTTGAAGTGAAATGCAATTA TTTTAACTTTCCTCATGGTAGTGACTCTGAGCCTTTTGGTGAAGATTTTCTAGATGCACAGAATACAAAC TGCCCCAAAGAGAAAATACCCAACCATCACAAAGAGGAGAAGCGTAATTCTCCATCTGAAAGAACATGTA CGCCTGTTACTCAAACCTTTGCTTCTGCTGGTAATAAAGTCCACCCTGTCTGCAGTGATGTTATTAAAAC TCAGCCATTACCTGGAAGTAGTATAACTCATGGCGCACTTCAGGAAGGCGACTCTTTGGATAAACTCTAT GCTCTTTGTGGTCAACATTGTCCGATACTAACTGTTATTATTCAACCTGTAAATGAGGAAGACCGAGGAT TTGCTTATCGAAAAGATTCCGATATTGAAAATTCTTTGGGTTTTCAGTTATGGATGGAAATATATCCATT TCTGCTACAGTCAAAGAAAAACATGTTCACAAGTGAGAGCAGGAAAGTGAATGTGGGCAAACAATTTACT GATAATGCCATTGGTAATCTGTGTGATCGACTTTATTTCAAAAACATGATTGACTTGGTGGATAAAAGAG CTAACTCTTTGAGGGAGGAAGTTAATTTGAACAAAGTTCAATTATATTTAAAGAAAAGGTTTTCAGATCT TTTGCCTTCATCGTCACTAGTGGTGTGGGATATGAATTCTGTTTCACTAACCCCCAGCAACTGGACAGAT AACTTCAAAAGTGTTGATGAGAACAACTTCATTAACAGATTCCAGAACTCAAGAAAAAATCCCAACCAAG TAGTAAGAGAAGAAAACAGTTGTCAATTCCATTGGGAGCTGTTTGGTCAAGTTTACTTGTTAGATATTTG CCAAGTTGAGAAATCCTTACATATCAAAACCAGAAGTAAATTAGAAATATATTACATTTTAGGGGGTGAA GTTCTTTTCATTTGGGAAGAAGAAAATCAATTAAATATAATAGATATTGAAAGCCCTAATGATCAGAATG ATTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217744 representing NM_001195676 Red=Cloning site Green=Tags(s) MSETPSTSFSMIRLTSSEGQVPSRHSNVTHPTVAKRISFYKSGDPQFGGVRVVVNPRSFKTFDALLDNLS RKVPLPFGVRNISTPRGRHSITKLEELEDGESYVCSHNKKVLPVDLDKARRRPRPWLSSRSISTHVKLRS ATATMPTTAPGLFRAPRRLVVFRNGDPKTRRVVLLSRRITQSFEAFLQYLTQVMQYPVAKLYATDGRKVP SLQAVILSSGAVVAAGREPFKPGNYDIQKYLLPVKLPGISHRVHRKGKAKIEKRKMSTHSVPRPQTDSLA SEKTYDCFSDFSVAPENYLALETHDSQTLSTYPSEDGVEKSIVFNQDGTMTVVMKVRFKIKEETVKWTTT VNRADLSNDDEKSKISSYPGKTEDRSSSLKLVVPCSLSEDITDTTQQGSLTEEENTQMTEQQALSSSSAG WENASMEVGIQGSQKQVKHFYRPPTPGPRRMRQKKSVIGTVTVVSETEVQEKQFSYREEREVGEKSKYHM FTHSCSKMSSVSNKLVQISSDDERESALERTKESGPLKSHAINAGAIEITSQRVLKMCHNNALASTVPEN SVVEEGTDDSAISGKATIKHFRTCGNANDSFSSTTADTTLTSGNNCPNVRTISEVPSVGSPVLTTGLVNE FAHCGLTEHPGNGRQISSPFASKKKKKSQQRMITSKDKKKVIETKGIPNKVGKIHRAGTIAQVVLLQDSG GLDKEGRVREEELDTSPMVIESHDIFPKGNSKFSKNFHKNKLNTVQNPKTQKLLAKRKSRPLKIKSIGEL RKQETGQEDKVFLHSESKLCESNLEKQSLLHVFSSLEEDQKALHRSQSQVETVTENWKGMTKKSLVPKVN DLHVTVRNQRKQKGVKLKSGATVSEQHIATRADPLASLKKPDLTEGIPHHSVKSYIQRWLQNINPYPDFE HRKSAPLGQDRSNVVNYNSNGFPENNLPTTSSKGKSFLMESNKRKTKNANWKGNKNQETSKSLVVKDNGE QPNKHSCESQDESLYDSYLGPLHDNCTLSQTSINDPCTKSHLSTEKSRPEVKLVYQEMNFSTKRQTLEVA IQVDTMGENVPKDLLALLLQHLEAFIPNNQKHQNGISQLPGSLAEVVFPSAIYNSSTNLLLAWLLVLNLK RNMNSFCQSDAPKTTSRSSETAALLDVLKHVAITEEADDLKAAVANLVESTKTCSGPSGREQDTAPANCI AASLHSVDKCKENKSTQKSLLDEGYSVRGDCGPELCVLEMVSKSNNTPSEGHTVSMTYPPKGVNNQNDAL FTSNDSSTMSQRSTHNASFLGEACLLTDSLCSHKACAQKDNIYEAACLSDETHIPIRDCKTIEFVNSKEN KCTDNLELTEEIKTVDKVPKDLNILADSIDKNESDISTSSQNVNKLSSQGIFLSKTHLDSDKDYSPLEEF QNCPPKKIVNKKKSVSSDKEDSRTSEEPRSITNSVTSSERNATSELESFEESESQDTGIFNMKVRTEEKA AKESMQKESEARMSLELINISGRNIIEQGRRNTAFLETTGRGQVTPPSLVFCYDSNKNTEKEMSVGETKM RVKKIVDSMENESYTDSSLNCNKQHRSPGTLDWSDYGSDSESRYPCKALSYEHSDDTGQEREYPRGIVRR AIEKLYGKAEIIKPPFFHGSIHKSQVCPYNPVEVPCVKKTHFYESECQSLVSSEQVSRSSLMLQEFQEER QSEVDANGMGDSFGDSSIEKVTKPSEHGRVFMEKEKGKLIDNGKWLLRENHLWRVSLDNPGMYGNADTTS VDTLMDKNSIEVPYSHFGELAPGPTMAELSSSEIEEMTQPLEVKCNYFNFPHGSDSEPFGEDFLDAQNTN CPKEKIPNHHKEEKRNSPSERTCTPVTQTFASAGNKVHPVCSDVIKTQPLPGSSITHGALQEGDSLDKLY ALCGQHCPILTVIIQPVNEEDRGFAYRKDSDIENSLGFQLWMEIYPFLLQSKKNMFTSESRKVNVGKQFT DNAIGNLCDRLYFKNMIDLVDKRANSLREEVNLNKVQLYLKKRFSDLLPSSSLVVWDMNSVSLTPSNWTD NFKSVDENNFINRFQNSRKNPNQVVREENSCQFHWELFGQVYLLDICQVEKSLHIKTRSKLEIYYILGGE VLFIWEEENQLNIIDIESPNDQNDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001195676
ORF Size	6375 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001195676.1, NP_001182605.1
RefSeq Size	6991 bp
RefSeq ORF	6378 bp
Locus ID	681377
MW	237.9 kDa
Gene Summary	This gene encodes a member of the doublecortin family. The protein encoded by this gene contains two doublecortin domains, which bind microtubules and regulate microtubule polymerization. The encoded protein is a photoreceptor microtubule-associated protein and is required for correct stacking of outer segment disc. This protein and the RP1L1 protein, another retinal-specific protein, play essential and synergistic roles in affecting photosensitivity and outer segment morphogenesis of rod photoreceptors. Because of its response to in vivo retinal oxygen levels, this protein was initially named ORP1 (oxygen-regulated protein-1). This protein was subsequently designated RP1 (retinitis pigmentosa 1) when it was found that mutations in this gene cause autosomal dominant retinitis pigmentosa. Mutations in this gene also cause autosomal recessive retinitis pigmentosa. [provided by RefSeq, Sep 2010]

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