Myof (NM_001191636) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Fer1l3; RGD1564216
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217730 representing NM_001191636 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTCGAGTGATTGTGGAATCTGCTACTAACATCCCTAAAACGAAATTTGGGAAACCGGATCCTATCG TTTCTGTCATTTTTAAGGATGAGAAAAAGAAAACAAAGAAAGTTGATAATGAACTCAACCCTGTCTGGAA TGAGATTTTGGAATTTGACTTGAGGGGGATACCACTGGACTCTTCATCTTCCCTGGTGATTGTTGTCAAA GATTTTGAGACGATTGGACAAAATAAATTAATTGGCACAGCAACTGTGTCCCTGAAGGACCTGATCGGGG ACCAGAACAGATCTCTGCCCTACAAACAGATCTCCCTGCTAAACGAGAAGGGACAAGACACGGGGGCCAC CATTGACCTGGTGATTGGCTATACCCCACCTTCAGCTCCATATCCAAATGATCCGAGTGGGACCAGTGTG ACAGGCGTGGGAGAAGAAGAGGAAGAAGACCAGGGGGATGAAGACAGGGTAGATGGCATGGTCAGAGGCC CTGGACCCAAGGGGCCATCTGGGACAATGTCAGAAGCTCAGCTTGCTCGGAGGATCACCAAAGGGAAGAG CAGCCGGAGGATGCTGTCCAACAAGCCACAGGATTTCCAGATCCGTGTCAGAGTGATTGAAGGCCGTCAG TTATGCGGCAACAACATAAGGCCTGTGGTGAAAGTTCACGTCTGTGGTCAGACACACCGCACGAGAATCA AGAGAGGGAACAATCCATTTTTTGATGAGTTATTTTTCTACAATGTGCACATCACACCCTCTGAACTCAT GGATGAGATCATCAGCATCCGGGTTTATAATTCCCACTCTCTGAGGGCAGATAGTCTGATGGGGGAATTT AAGATTGATGTTGGATTTGTTTACGATGAACCTGGCCATGCTGTCATGAGGAAATGGCTCCTTCTCAATG ACCCAGAAGACACCAGCTCAGGTGCAAAGGGGTACATGAAAGTCAGCATGTTTGTCCTGGGAACCGGAGA CGAACCCCCATCTGAGAAACGAGACCGGGACAATGACAGTGATGACGTCGAGAGCAACTTGTTACTTCCA GCTGGCATTGCCCTCCGGTGGGTAACCTTCTTGCTGAAGATCTACCGAGCTGAGGACATCCCCCAGATGG ATGATGCCTTCTCACAGACAGTGAAGGAAATATTTGGTGGCAATGCTGATAAAAAAAATCTCGTGGACCC TTTCGTAGAGGTCTCCTTTGCTGGGAAAAAGGTGTGTACAAATATTATTGAGAGAAATGCAAACCCCGAA TGGAATCAAGTTGTCAATCTTCAGATCAAGTTTCCTTCCATGTGTGAAAAAATAAAACTAACAGTGTATG ACTGGGACCGGCTTACCAAAAATGATGTAGTTGGGACGACATACCTGTACCTCTCTAAAATTGCTGCCTC TGGGGGAGAAGTGGAAGCAAATACAGGAGAGACTGAGGTGGGTTTCGTCCCAACATTTGGACCTTGTTAT TTGAACCTTTATGGAAGCCCCAGAGAGTACACAGGATTTCCAGATCCCTACGATGAGCTGAATTCAGGAA AGGGAGAAGGGGTGGCCTACAGAGGCAGGATCTTGGTTGAACTGAACACGTTCCTTGAAAAGAAACCACC AGAAAAGAAGCTTGAGCCCATTTCAAATGATGACCTTCTGGTTGTTGAGAAATACCAGCGACGGCGGAAG TACAGCCTGTCGGCCGTGTTCCATTCAGCCACCATGTTGCAAGATGTTGGCGAGGCCATTCAGTTTGAAG TCAGCATTGGGAACTATGGCAACAAGTTTGATGCAACCTGTAAACCTCTGGCATCAACAACTCAATACAG CCGTGCAGTATTTGATGGGAACTACTATTACTATTTACCTTGGGCCCGCACAAAGCCTGTTGTCACCCTG ACTTCCTACTGGGAGGATATTAGTCATCGCCTGGATGCAGTGAACACTCTCCTAGTCATGGCAGAACGGC TGCAATCAAACATCGAGGCTGTAAAATCAGGAATCCAAGGTAAAATTCCCACAAACCAGTTGGCAGAGCT GTGGCTGAAGCTAATAGACGAAGTTATAGAAGACACAAGATACACCTTGCCTGTCACTGAAGGAAAAGCC AACGTCACAGTTCTGGATACTCAGATCCGAAAGTTGCGGCTGAGGTTTCTCTCGCAGATACACGAGGCGG CTCTGAGGATGAGATCTGAAGCCACGGATGTGAAATCCACCTTGCTGGAGATCGAGGAGTGGCTTGATAA ATTAATGCAACTTACTGAAGAGCCTCAGAACAGTATGCCCGACATCATCATCTGGATGATCCGCGGGGAG AAAAGACTGGCTTATGCACGGATTCCTGCCCACCAAGTCTTATACTCCACCAGTGGTGGGAACGCCTCCG GAAAATACTGTGGGAAAACCCAAACCATCCTTCTGAAGTATCCACAAGAAAAGAACAATGGGCCCAAAGT GCCTGTGGAATTGCGAGTGAACATCTGGCTGGGTTTAAGCGCCGTGGAAAAGAAGTTCAATAGCTTCGCT GAAGGCACATTCACCGTCTTTGCTGAAATGTATGAAAACCAAGCCCTCGTGTTTGGGAAGTGGGGCACCT CTGGACTGGTGGGACGCCATAAATTCTCTGATGTCACAGGAAAAATAAAACTCAAAAGAGAATTCTTTTT ACCTCCCAAAGGCTGGGAATGGGAAGGAGACTGGGTAGTTGACCCTGAACGAAGCCTCCTGACTGAGGCT GATGCAGGTCACACAGAGTTCACTGATGAAGTGTACCAGAACGAGACTCGCTATCCTGGAGGCGAGTGGA AGCAGGCAGAGGACACCTATACCCGATGCGACGCGAATGGAGATAAAGCAGCATCACCCAATGAGATGAC TTGCCCTCCGGGTTGGGAGTGGGAAGATGACGCATGGACATATGACATAAATCGAGCAGTGGACGAGAAA GGCTGGGAGTATGGAATTACCATTCCTCCTGACAATAAGCCCAAATCCTGGGTCGCAGCAGAGAAGATGT ATCACACTCACAGGCGGCGGAGACTGGTCCGAAAGCGCAAGAAAGATTTGACACAGACGGCTTCCAGCAC TGCGAGGGCCATGGAAGAACTTGAAGACCGGGAGGGCTGGGAATACGCCTCGTTAATTGGCTGGAAATTC CACTGGAAACAGCGCAGTTCAGATACCTTTCGCCGCAGGCGCTGGAGGAGAAAAATGGCTCCTTCAGAGA CACATGGCGCAGCGGCCATCTTTAAACTTGAAGGTGCCCTTGGGGCAGACACCACAGAGGATGGAGATGA AAAGAGCCTAGAGAAGCAGAAGCACAGTGCCACCACTGTGTTTGGAGCAAACACACCCATTGTTTCCTGT AATTTCGACAGAGTCTACATCTACCACCTACGCTGCTACATTTATCAAGCCCGGAACCTCATGGCTTTGG ACAAAGACAGCTTTTCAGACCCGTATGCTCATGTCTCCTTCCTCCATCGAAGCAAAACCACTGAGATCAT TCACTCCACCCTGAATCCCACCTGGGACCAAACCATTATATTTGATGACGTTGAAATCTTTGGGGAGCCC CAAACAGTTCTGCAGAACCCGCCCAAAGTCATCATCGAACTTTTTGACAGTGACCAAGTGGGCAAAGATG AGTTTTTAGGTCGAAGCATTTGCTCTCCTCTGGTGAAACTGAACTCAGAAACAGACACCACACCTAAGCT TCTCTGGCACCCAGTTATGAATGGAGATAAGGCCTGTGGGGATGTCCTTGTAACAGCAGAACTGATTCTG AGGAACAAGGACGGCTCCAACCTTCCCATCCTCCCCTCTCAAAGAGCACCAAACTTATACATGGTCCCTC AAGGGATCAGACCTGTGGTTCAGCTCACCGCTATTGAGATCCTAGCTTGGGGCTTAAGAAATATGAAAAA CTACCAGATGGCTTCCATCACCTCGCCCAGCCTCGTGGTGGAGTGCGGAGGAGAGAGAGTAGAATCGGTG GTGATCAAAAGCCTCAAGAAGACACCCAACTTCCCAAGTTCTGTTCTCTTCATGAAAGTGTTCTTGCCCA AGGAAGAATTGTACATGCCACCACTGGTGATCAAGGTCATTGATCACAGGCAGTTCGGGCGGAAGCCTGT GGTCGGCCAATGCACCATTGACCACCTGGACCGCTTTCGGTGTGATCCATATGCAGGAAAGGAGGACATT GTACCGCAACTAAAAGCTTCCCTTATGTCTGCACCCCCATGCCGGGATGTCATTATTGAAATAGAAGACA CCAAACCATTGCTGGCTTCTAAGCTGTCAGAAAAGGAGGAAGAAATCGTTGACTGGTGGAGTAAATTTTA TGCTTCCTCGGGGGAGCATGAAAAATGTGGACAGTATATTCAGAAAGGCTATTCCAAACTCAAGATCTAT GACTGTGAACTGGAGGACGTAGCTGACTTTGAAGGCCTGACAGATTTCTCAGATACCTTCAAGTTGTACA GAGGCAAGTCCGATGAGAATGAAGATCCGTCCGTGGTAGGAGAGTTTAAAGGCTCCTTCCGAATCTACCC CTTGCCGGATGACCCCAGTGTGCCGGCCCCTCCCAGGCAGTTTCGGGAACTGCCTGACAGTGTCCCGCAG GAATGCACGGTTAGGATTTACATTGTTCAAGGCTTACAGCTCCAGCCCCAGGACAACAATGGCCTGTGTG ATCCTTACATAAAAATAACCCTGGGCAAAAAAGTAATTGAAGACAGAGACCATTACATTCCCAACACTAT CAACCCAGTGTTCGGCAGAATGTATGAACTGAGCTGCTACTTACCTCAAGAGAAAGACCTGAAGATCTCT GTCTATGACTACGACACCTTCACCCGAGATGAGAAGGTGGGAGAGACAACCATTGACCTGGAGAACCGCT TCCTGTCCCGGTTCGGGTCTCACTGCGGCATCCCTGAGCAGTACTGTGTTTCTGGAGTGAACACCTGGCG AGATCAGCTGAGACCGACACAGTTGCTTCAAAACGTAGCCAGATTCAAAGGCTTCCCGCCACCAGTCCTT TCTGAAGATGGAAGCAGGATCCAATATGGAGGAAGAGACTATCATCTGGATGAGTTTGAGGCCAACAAAA TCCTGCACCAGCACCTTGGAGCCCCTGAAGAGCGTCTTGCCCTCCATATTCTCAGGACTCAGGGCCTGGT CCCTGAGCACGTGGAGACAAGGACTTTGCACAGCACCTTCCAGCCCAATATTTCCCAGGGAAAACTTCAG ATGTGGGTGGATGTCTTCCCCAAGAGTCTAGGACCACCAGGTCCCCCTTTCAACATCACTCCCAGGAAAG CCAAGACGTACTATCTGCGTGTCATCATCTGGAATACCAGGGACGTTATCTTGGATGAGAAGAGCATCAC TGGGGAGGACATGAGCGACATCTACGTCAAAGGCTGGATTTCCGGCAGCGAAGAAAACAAACAGAAAACA GATGTCCACTACAGGTCCTTGGATGGCGAAGGCAATTTTAACTGGAGATTTGTTTTCCCATTTGACTACC TCCCCGCTGAGCAGCTCTGTATCGTCGCTAAAAAAGAGCATTTCTGGAGTATTGATCAAACAGAATTCCG AGTGCCCCCTCGGCTGATCATTCAGATATGGGACAATGACAAGTTTTCCCTGGACGACTACTTGGGTTTT CTGGAACTCGACTTGCATCGTACGATCATCCCAGCCAAGACCTCGGAGAAATGCAGTTTGGACATGATTC CAGACCTCAAAGCCATGGACCCTCTTAAGGCTAAGACTGCTTCTCTCTTTGAACAGAAGTCCATGAAAGG CTGGTGGCCATGCTACGCAGACAAAGATGGCTCCCGCGTGATGGCTGGGAAAGTGGAGATGACACTGGAG GTCCTCAACGAGAGGGAGGCCGACGAGAGGCCAGCCGGGAAAGGGCGGAGTGAACCCAACATGAACCCCA AGCTTGACCCACCAAACCGACCGGAAACCTCCTTCCTCTGGTTCACGAACCCTTGCAAGACCATGCGCTT CATCGTGTGGCGCCGGTTCAAGTGGGTGATCATCGGCCTGCTTCTGCTGCTCATCCTGCTGCTGTTCGTG GCTGTGCTCCTGTACTCGCTGCCGAACTATTTATCCATGAAGATTGTGAGGCCAAATGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217730 representing NM_001191636 Red=Cloning site Green=Tags(s) MLRVIVESATNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDSSSSLVIVVK DFETIGQNKLIGTATVSLKDLIGDQNRSLPYKQISLLNEKGQDTGATIDLVIGYTPPSAPYPNDPSGTSV TGVGEEEEEDQGDEDRVDGMVRGPGPKGPSGTMSEAQLARRITKGKSSRRMLSNKPQDFQIRVRVIEGRQ LCGNNIRPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVHITPSELMDEIISIRVYNSHSLRADSLMGEF KIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGAKGYMKVSMFVLGTGDEPPSEKRDRDNDSDDVESNLLLP AGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIERNANPE WNQVVNLQIKFPSMCEKIKLTVYDWDRLTKNDVVGTTYLYLSKIAASGGEVEANTGETEVGFVPTFGPCY LNLYGSPREYTGFPDPYDELNSGKGEGVAYRGRILVELNTFLEKKPPEKKLEPISNDDLLVVEKYQRRRK YSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDATCKPLASTTQYSRAVFDGNYYYYLPWARTKPVVTL TSYWEDISHRLDAVNTLLVMAERLQSNIEAVKSGIQGKIPTNQLAELWLKLIDEVIEDTRYTLPVTEGKA NVTVLDTQIRKLRLRFLSQIHEAALRMRSEATDVKSTLLEIEEWLDKLMQLTEEPQNSMPDIIIWMIRGE KRLAYARIPAHQVLYSTSGGNASGKYCGKTQTILLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFA EGTFTVFAEMYENQALVFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGDWVVDPERSLLTEA DAGHTEFTDEVYQNETRYPGGEWKQAEDTYTRCDANGDKAASPNEMTCPPGWEWEDDAWTYDINRAVDEK GWEYGITIPPDNKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELEDREGWEYASLIGWKF HWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGDEKSLEKQKHSATTVFGANTPIVSC NFDRVYIYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDDVEIFGEP QTVLQNPPKVIIELFDSDQVGKDEFLGRSICSPLVKLNSETDTTPKLLWHPVMNGDKACGDVLVTAELIL RNKDGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASITSPSLVVECGGERVESV VIKSLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIDHLDRFRCDPYAGKEDI VPQLKASLMSAPPCRDVIIEIEDTKPLLASKLSEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIY DCELEDVADFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQ ECTVRIYIVQGLQLQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTINPVFGRMYELSCYLPQEKDLKIS VYDYDTFTRDEKVGETTIDLENRFLSRFGSHCGIPEQYCVSGVNTWRDQLRPTQLLQNVARFKGFPPPVL SEDGSRIQYGGRDYHLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQGKLQ MWVDVFPKSLGPPGPPFNITPRKAKTYYLRVIIWNTRDVILDEKSITGEDMSDIYVKGWISGSEENKQKT DVHYRSLDGEGNFNWRFVFPFDYLPAEQLCIVAKKEHFWSIDQTEFRVPPRLIIQIWDNDKFSLDDYLGF LELDLHRTIIPAKTSEKCSLDMIPDLKAMDPLKAKTASLFEQKSMKGWWPCYADKDGSRVMAGKVEMTLE VLNEREADERPAGKGRSEPNMNPKLDPPNRPETSFLWFTNPCKTMRFIVWRRFKWVIIGLLLLLILLLFV AVLLYSLPNYLSMKIVRPNA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191636
ORF Size	6150 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191636.1, NP_001178565.1
RefSeq Size	7235 bp
RefSeq ORF	6153 bp
Locus ID	309499
MW	233.6 kDa

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