Myof (NM_001191636) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Fer1l3; RGD1564216
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217730 representing NM_001191636 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTCGAGTGATTGTGGAATCTGCTACTAACATCCCTAAAACGAAATTTGGGAAACCGGATCCTATCG TTTCTGTCATTTTTAAGGATGAGAAAAAGAAAACAAAGAAAGTTGATAATGAACTCAACCCTGTCTGGAA TGAGATTTTGGAATTTGACTTGAGGGGGATACCACTGGACTCTTCATCTTCCCTGGTGATTGTTGTCAAA GATTTTGAGACGATTGGACAAAATAAATTAATTGGCACAGCAACTGTGTCCCTGAAGGACCTGATCGGGG ACCAGAACAGATCTCTGCCCTACAAACAGATCTCCCTGCTAAACGAGAAGGGACAAGACACGGGGGCCAC CATTGACCTGGTGATTGGCTATACCCCACCTTCAGCTCCATATCCAAATGATCCGAGTGGGACCAGTGTG ACAGGCGTGGGAGAAGAAGAGGAAGAAGACCAGGGGGATGAAGACAGGGTAGATGGCATGGTCAGAGGCC CTGGACCCAAGGGGCCATCTGGGACAATGTCAGAAGCTCAGCTTGCTCGGAGGATCACCAAAGGGAAGAG CAGCCGGAGGATGCTGTCCAACAAGCCACAGGATTTCCAGATCCGTGTCAGAGTGATTGAAGGCCGTCAG TTATGCGGCAACAACATAAGGCCTGTGGTGAAAGTTCACGTCTGTGGTCAGACACACCGCACGAGAATCA AGAGAGGGAACAATCCATTTTTTGATGAGTTATTTTTCTACAATGTGCACATCACACCCTCTGAACTCAT GGATGAGATCATCAGCATCCGGGTTTATAATTCCCACTCTCTGAGGGCAGATAGTCTGATGGGGGAATTT AAGATTGATGTTGGATTTGTTTACGATGAACCTGGCCATGCTGTCATGAGGAAATGGCTCCTTCTCAATG ACCCAGAAGACACCAGCTCAGGTGCAAAGGGGTACATGAAAGTCAGCATGTTTGTCCTGGGAACCGGAGA CGAACCCCCATCTGAGAAACGAGACCGGGACAATGACAGTGATGACGTCGAGAGCAACTTGTTACTTCCA GCTGGCATTGCCCTCCGGTGGGTAACCTTCTTGCTGAAGATCTACCGAGCTGAGGACATCCCCCAGATGG ATGATGCCTTCTCACAGACAGTGAAGGAAATATTTGGTGGCAATGCTGATAAAAAAAATCTCGTGGACCC TTTCGTAGAGGTCTCCTTTGCTGGGAAAAAGGTGTGTACAAATATTATTGAGAGAAATGCAAACCCCGAA TGGAATCAAGTTGTCAATCTTCAGATCAAGTTTCCTTCCATGTGTGAAAAAATAAAACTAACAGTGTATG ACTGGGACCGGCTTACCAAAAATGATGTAGTTGGGACGACATACCTGTACCTCTCTAAAATTGCTGCCTC TGGGGGAGAAGTGGAAGCAAATACAGGAGAGACTGAGGTGGGTTTCGTCCCAACATTTGGACCTTGTTAT TTGAACCTTTATGGAAGCCCCAGAGAGTACACAGGATTTCCAGATCCCTACGATGAGCTGAATTCAGGAA AGGGAGAAGGGGTGGCCTACAGAGGCAGGATCTTGGTTGAACTGAACACGTTCCTTGAAAAGAAACCACC AGAAAAGAAGCTTGAGCCCATTTCAAATGATGACCTTCTGGTTGTTGAGAAATACCAGCGACGGCGGAAG TACAGCCTGTCGGCCGTGTTCCATTCAGCCACCATGTTGCAAGATGTTGGCGAGGCCATTCAGTTTGAAG TCAGCATTGGGAACTATGGCAACAAGTTTGATGCAACCTGTAAACCTCTGGCATCAACAACTCAATACAG CCGTGCAGTATTTGATGGGAACTACTATTACTATTTACCTTGGGCCCGCACAAAGCCTGTTGTCACCCTG ACTTCCTACTGGGAGGATATTAGTCATCGCCTGGATGCAGTGAACACTCTCCTAGTCATGGCAGAACGGC TGCAATCAAACATCGAGGCTGTAAAATCAGGAATCCAAGGTAAAATTCCCACAAACCAGTTGGCAGAGCT GTGGCTGAAGCTAATAGACGAAGTTATAGAAGACACAAGATACACCTTGCCTGTCACTGAAGGAAAAGCC AACGTCACAGTTCTGGATACTCAGATCCGAAAGTTGCGGCTGAGGTTTCTCTCGCAGATACACGAGGCGG CTCTGAGGATGAGATCTGAAGCCACGGATGTGAAATCCACCTTGCTGGAGATCGAGGAGTGGCTTGATAA ATTAATGCAACTTACTGAAGAGCCTCAGAACAGTATGCCCGACATCATCATCTGGATGATCCGCGGGGAG AAAAGACTGGCTTATGCACGGATTCCTGCCCACCAAGTCTTATACTCCACCAGTGGTGGGAACGCCTCCG GAAAATACTGTGGGAAAACCCAAACCATCCTTCTGAAGTATCCACAAGAAAAGAACAATGGGCCCAAAGT GCCTGTGGAATTGCGAGTGAACATCTGGCTGGGTTTAAGCGCCGTGGAAAAGAAGTTCAATAGCTTCGCT GAAGGCACATTCACCGTCTTTGCTGAAATGTATGAAAACCAAGCCCTCGTGTTTGGGAAGTGGGGCACCT CTGGACTGGTGGGACGCCATAAATTCTCTGATGTCACAGGAAAAATAAAACTCAAAAGAGAATTCTTTTT ACCTCCCAAAGGCTGGGAATGGGAAGGAGACTGGGTAGTTGACCCTGAACGAAGCCTCCTGACTGAGGCT GATGCAGGTCACACAGAGTTCACTGATGAAGTGTACCAGAACGAGACTCGCTATCCTGGAGGCGAGTGGA AGCAGGCAGAGGACACCTATACCCGATGCGACGCGAATGGAGATAAAGCAGCATCACCCAATGAGATGAC TTGCCCTCCGGGTTGGGAGTGGGAAGATGACGCATGGACATATGACATAAATCGAGCAGTGGACGAGAAA GGCTGGGAGTATGGAATTACCATTCCTCCTGACAATAAGCCCAAATCCTGGGTCGCAGCAGAGAAGATGT ATCACACTCACAGGCGGCGGAGACTGGTCCGAAAGCGCAAGAAAGATTTGACACAGACGGCTTCCAGCAC TGCGAGGGCCATGGAAGAACTTGAAGACCGGGAGGGCTGGGAATACGCCTCGTTAATTGGCTGGAAATTC CACTGGAAACAGCGCAGTTCAGATACCTTTCGCCGCAGGCGCTGGAGGAGAAAAATGGCTCCTTCAGAGA CACATGGCGCAGCGGCCATCTTTAAACTTGAAGGTGCCCTTGGGGCAGACACCACAGAGGATGGAGATGA AAAGAGCCTAGAGAAGCAGAAGCACAGTGCCACCACTGTGTTTGGAGCAAACACACCCATTGTTTCCTGT AATTTCGACAGAGTCTACATCTACCACCTACGCTGCTACATTTATCAAGCCCGGAACCTCATGGCTTTGG ACAAAGACAGCTTTTCAGACCCGTATGCTCATGTCTCCTTCCTCCATCGAAGCAAAACCACTGAGATCAT TCACTCCACCCTGAATCCCACCTGGGACCAAACCATTATATTTGATGACGTTGAAATCTTTGGGGAGCCC CAAACAGTTCTGCAGAACCCGCCCAAAGTCATCATCGAACTTTTTGACAGTGACCAAGTGGGCAAAGATG AGTTTTTAGGTCGAAGCATTTGCTCTCCTCTGGTGAAACTGAACTCAGAAACAGACACCACACCTAAGCT TCTCTGGCACCCAGTTATGAATGGAGATAAGGCCTGTGGGGATGTCCTTGTAACAGCAGAACTGATTCTG AGGAACAAGGACGGCTCCAACCTTCCCATCCTCCCCTCTCAAAGAGCACCAAACTTATACATGGTCCCTC AAGGGATCAGACCTGTGGTTCAGCTCACCGCTATTGAGATCCTAGCTTGGGGCTTAAGAAATATGAAAAA CTACCAGATGGCTTCCATCACCTCGCCCAGCCTCGTGGTGGAGTGCGGAGGAGAGAGAGTAGAATCGGTG GTGATCAAAAGCCTCAAGAAGACACCCAACTTCCCAAGTTCTGTTCTCTTCATGAAAGTGTTCTTGCCCA AGGAAGAATTGTACATGCCACCACTGGTGATCAAGGTCATTGATCACAGGCAGTTCGGGCGGAAGCCTGT GGTCGGCCAATGCACCATTGACCACCTGGACCGCTTTCGGTGTGATCCATATGCAGGAAAGGAGGACATT GTACCGCAACTAAAAGCTTCCCTTATGTCTGCACCCCCATGCCGGGATGTCATTATTGAAATAGAAGACA CCAAACCATTGCTGGCTTCTAAGCTGTCAGAAAAGGAGGAAGAAATCGTTGACTGGTGGAGTAAATTTTA TGCTTCCTCGGGGGAGCATGAAAAATGTGGACAGTATATTCAGAAAGGCTATTCCAAACTCAAGATCTAT GACTGTGAACTGGAGGACGTAGCTGACTTTGAAGGCCTGACAGATTTCTCAGATACCTTCAAGTTGTACA GAGGCAAGTCCGATGAGAATGAAGATCCGTCCGTGGTAGGAGAGTTTAAAGGCTCCTTCCGAATCTACCC CTTGCCGGATGACCCCAGTGTGCCGGCCCCTCCCAGGCAGTTTCGGGAACTGCCTGACAGTGTCCCGCAG GAATGCACGGTTAGGATTTACATTGTTCAAGGCTTACAGCTCCAGCCCCAGGACAACAATGGCCTGTGTG ATCCTTACATAAAAATAACCCTGGGCAAAAAAGTAATTGAAGACAGAGACCATTACATTCCCAACACTAT CAACCCAGTGTTCGGCAGAATGTATGAACTGAGCTGCTACTTACCTCAAGAGAAAGACCTGAAGATCTCT GTCTATGACTACGACACCTTCACCCGAGATGAGAAGGTGGGAGAGACAACCATTGACCTGGAGAACCGCT TCCTGTCCCGGTTCGGGTCTCACTGCGGCATCCCTGAGCAGTACTGTGTTTCTGGAGTGAACACCTGGCG AGATCAGCTGAGACCGACACAGTTGCTTCAAAACGTAGCCAGATTCAAAGGCTTCCCGCCACCAGTCCTT TCTGAAGATGGAAGCAGGATCCAATATGGAGGAAGAGACTATCATCTGGATGAGTTTGAGGCCAACAAAA TCCTGCACCAGCACCTTGGAGCCCCTGAAGAGCGTCTTGCCCTCCATATTCTCAGGACTCAGGGCCTGGT CCCTGAGCACGTGGAGACAAGGACTTTGCACAGCACCTTCCAGCCCAATATTTCCCAGGGAAAACTTCAG ATGTGGGTGGATGTCTTCCCCAAGAGTCTAGGACCACCAGGTCCCCCTTTCAACATCACTCCCAGGAAAG CCAAGACGTACTATCTGCGTGTCATCATCTGGAATACCAGGGACGTTATCTTGGATGAGAAGAGCATCAC TGGGGAGGACATGAGCGACATCTACGTCAAAGGCTGGATTTCCGGCAGCGAAGAAAACAAACAGAAAACA GATGTCCACTACAGGTCCTTGGATGGCGAAGGCAATTTTAACTGGAGATTTGTTTTCCCATTTGACTACC TCCCCGCTGAGCAGCTCTGTATCGTCGCTAAAAAAGAGCATTTCTGGAGTATTGATCAAACAGAATTCCG AGTGCCCCCTCGGCTGATCATTCAGATATGGGACAATGACAAGTTTTCCCTGGACGACTACTTGGGTTTT CTGGAACTCGACTTGCATCGTACGATCATCCCAGCCAAGACCTCGGAGAAATGCAGTTTGGACATGATTC CAGACCTCAAAGCCATGGACCCTCTTAAGGCTAAGACTGCTTCTCTCTTTGAACAGAAGTCCATGAAAGG CTGGTGGCCATGCTACGCAGACAAAGATGGCTCCCGCGTGATGGCTGGGAAAGTGGAGATGACACTGGAG GTCCTCAACGAGAGGGAGGCCGACGAGAGGCCAGCCGGGAAAGGGCGGAGTGAACCCAACATGAACCCCA AGCTTGACCCACCAAACCGACCGGAAACCTCCTTCCTCTGGTTCACGAACCCTTGCAAGACCATGCGCTT CATCGTGTGGCGCCGGTTCAAGTGGGTGATCATCGGCCTGCTTCTGCTGCTCATCCTGCTGCTGTTCGTG GCTGTGCTCCTGTACTCGCTGCCGAACTATTTATCCATGAAGATTGTGAGGCCAAATGCATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191636
Insert Size	6153 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191636.1, NP_001178565.1
RefSeq Size	7235 bp
RefSeq ORF	6153 bp
Locus ID	309499

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