Arhgap21 (NM_001191693) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217714 representing NM_001191693 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGCCACACATTGGACTGGTCTGCCTGAGGGAGATGGTGACAAACTCAAGGCCTGTGGAGCCGCCT GTGAGGTGTCAAAAAATAAAGATGGAAAGGAACAGGGTGAACCCGTGTCACCGTCCGAAGACGAATCGTT CTCCTGGCCAGGTCCCAAGACAGTCATGCTGAAGAGAACATCTCAAGGATTCGGCTTCACACTGCGACAC TTCATCGTCTATCCCCCGGAGTCTGCAATCCAGTTCTCATACAAGGATGAAGAGAATGGAAACAGAGGAG GAAAGCAAAGGAACCGATTGGAGCCGATGGACACCATATTTGTTAAACAAGTGAAGGAAGGAGGGCCAGC CTTCGAGGCTGGGTTATGCACAGGTGACAGAATTATAAAAGTCAATGGAGAAAGTGTCATAGGAAAGACC TATTCTCAAGTAATTGCTCTCATTCAGAACAGTGACACCACACTGGAGCTCAGTGTTATGCCAAAGGATG AAGACATTCTCCAAGTGCTACAGTTTACAAAGGATGTCACAGCACTGGCGTACTCTCAAGATGCCTACCT GAAAGGCAACGGAGCTTACAGCGGCAATGCCCGTGACATTCCCGAGCCTCCACCAGTTTGCTATCCCTGG CTCCCGGCTACTCCGTCAGCCACGGCACAGCCAGTTGAAGTGTCTCCTCCCGACTCGTTACTGAATAAAC AGCAAACCAGTACACCAGTCCTGACACAACCGGGCAGGGCCTATAGAATGGAAATACAAGTGCCTCCATC ACCAACAGATGTTGCAAAGTCAAACACGGCCGTGTGTGTTTGCAGTGAGAGTGTGAGGACCGTCATTGTG CCTTCTGAGAAGGTGGTGGACTTGCTAGCCAATAGAAACAGCCCTTCAGGCCCTGCACATAGAACGGAAG AGGTAAGGTATGGTGTAAGTGACCAGGCCTCTGCAAAAGCAGCAGCCAGGACCACGCCTCCGCCATCTGC TCCCACTGCCCGTCTGAGTCATCAGACCATAGGCTCCAGGTCACTGGAACCTTCTGGAATTTTACTTAAA TCTGGAAATTACAGCGGACATTCAGAAGGAATCTCAAGTAGTAGATCACAAGCTGTGGATTCTCCCCCTG TGTCTGTCAATCACTATTCCGCAAACTCCCATCAGCACATAGACTGGAAAAACTATAAGACTTACAAAGA ATACATTGATAACAGGAGATTGCACATAGGTTGTCGGACAATCCAGGAGAGGTTGGACAGCTTGCGAGCT GCATCTCAGAGCGCAGCAGACTATAACCAGGTGGTGCCCGCCCGCACCGCCCTGCAGGTACGACGTCGAA GCACCTCTCATGACCGGGTGCCCCAGTCCATTCAGATCCGGCAGCGCAGTGTATCTCAGGAAAGACTGGA AGATTCCGTGCTGATGAAGTATTGCCCGAGGAGCGCATCTCAAGGAGCGCTGACCTCCCCTCCTGTTAGC TTCAGCAACCATAGAACTCGCTCGTGGGATTACATCGAGGGGCAGAGTGAAGCCACTGCCACTGTCAATT CTGAAAGTCAGACGCCCGACTCAAACGGGGAACGAAAACAGACTTACAAGTGGAGTGGCTTCACCGAGCA GGATGACCGACGGGGCATTCATGAGAGACCCAGGCAGCAAGAAATGCACAAACCTTTCCGAGGCTCAAAT TTGACTGTGGCCCCCGTTGTTAATTCTGATAACAGGCGACTGGGTGGGAGAGGGGTGGGACCTGTTTCAC AGTTTAAAAAAATCCCACCGGACCTAAGACCACCACATTCCAACAGAAATTTTCCAACCACGAGTGGGAT GTCACTGCCACGAGGTATTGCACAAGACAGGTCTCCACTTGTGAAAGTCCGAAGTAATTCTCTGAAAGCG CTGCCTCCACCTGTCCCAAAGCCATCCTTCAGCCAGCACTCCCTGGCCTCCACGAAAGACCAAAGGCCTG TCAACCATCTGCACCAACACGGCCTGCTGAGTCAGCAGACACGGGGCCGACCCGAAAGCACCTTCCCTCA CCTCGAGCTGCAGACCGACGTCTCCCCGAGCTTACCAGGAACCTCTGCCAAGACCAGTCCTCAGCTCAGT GAGAACCTGGGGACGTCAGACTTAGACTTACATGCCAGTCCACGGAATGGAGATGTAGACTCACAGGAGG CTGACGTTCAGCCGTCAGATGTACCAGATAATAAAGAGTCTGTCATCCTGAGAGAGAAACCTCAGCCTGG CCGCCAGACGCCACAGCCTTTAAGACATCAGTCTTACATCTTGGCAGTGAATGACCAGGAGACCGGGTCA GACCCCACCTGCTGGCTGCCAAATGATGCTCGCAGAGAGGTCCACATCAAAAGAATGGAGGAGAGGAAGG CCTCCTGCACCAGCCCTCCTGGCGATTCCCTGGCTTCCATACCTTTTATAGACGAACCGACCAGCCCTAG CATCGACCACGAGGTCGCACACATCCCTGCCTCTGCTGTCATCTCTGCCTCTACCTCCCAGGTCCCCTCC ATTGCAACTGTTCCCCCCAGCCTCACCACCTCTGCTCCTCTGATTCGACGTCAGCTCTCCCATGACCAAG AATCTGTTGGACCTCCCAGCCTGGATGGCCAGCACACCTCAAAGACAGAAAGATCAAAATCCTATGATGA AGGCCTGGATGATTACAGAGAAGACGCAAAGTTATCCTTTAAGCACGTGTCTAGCCTGAAGGGAATCAAG ATTGCAGACAGCCAGAAGTCATCAGAAGACTCTGGGTCCAGAAAGGATTCTTCCTCCGAGGTCTTCAGTG ATGCAGCCAAAGAAGGGTGGCTCCAGTTCCGGCCGCTCGTCACCGATAAGGGCAAGAGAGTCGGTGGCAG TATTCGGCCATGGAAACAGATGTATGTTGTCCTTCGGGGACATTCACTTTACTTGTACAAAGACAGACGA GAGCAGACGACGCCATCAGAAGAGGAGCAGCCCATCAGTGTCAACGCTTGCCTGATAGACATCTCGTACA GTGAGACCAAGAGGAGGAACGTGTTCCGACTCACCACGTCCGACTGCGAATGCTTGTTCCAGGCTGAAGA CAGAGACGATATGTTAGCATGGATCAAGACTATTCAGGAGAGTAGCAACCTAAATGAAGAGGACACTGGA GTGACCAACAGAGATCTAATTAGTCGAAGAATCAAAGAGTATAACAGTCTGCTGAGCAAAACAGAACAGT TGCCAAAAACACCTCGCCAGAGTCTTAGCATCCGGCAAACTCTGCTCGGCGCTAAATCAGAGCCAAAGAC TCAAAGCCCACATTCTCCAAAGGAGGAGTCGGAGAGGAAGCTTCTCAGTAAAGATGATACCAGTCCTCCG AAAGACAAAGGTACTTGGAGAAGAGGCATTCCGAGCATCGTGAGGAAGACGTTTGAGAAAAAGCCGGCTG CCACAGGGACCTTCGGAGTTAGGCTGGACGATTGCCCGCCTGCCCACACCAACCGGTATATTCCATTAAT CGTTGACATATGTTGCAAATTGGTTGAAGAAAGGGGTCTTGAATATACAGGTATTTACCGAGTTCCCGGC AACAACGCAGCCATTTCAAGCATGCAGGAAGAGCTCAACAAGGGGATGGCAGACATTGACATACAAGATG ACAAATGGCGAGATTTGAATGTGATCAGCAGTTTACTAAAATCCTTCTTCAGAAAGCTCCCCGAGCCTCT CTTTACTAACGACAAATATGCTGACTTCATCGAGGCCAATCGCAAGGAGGACCCTCTAGATCGGCTGAGG ACGCTAAAGAGACTAATTCACGATCTGCCTGAGCATCATTTCGAGACACTGAAGTTTCTCTCGGCCCATC TGAAGACAGTGGCAGAAAACTCAGAAAAAAATAAGATGGAACCCAGAAACCTAGCGATTGTTTTCGGTCC AACTCTAGTGAGAACATCAGAAGACAACATGACCCACATGGTCACACACATGCCTGACCAGTACAAGATC GTAGAGACCCTCATCCAGCACCATGACTGGTTTTTCACAGAAGAGGGTGCTGAAGAGCCTCTCACAGCAG TGCAGGAGGAGAACACAGTAGACTCCCAGCCAGTACCAAACATAGATCACTTACTCACCAACATTGGAAG GACAGGCGTGCTCCCTGGAGATGTATCAGATTCAGCCACTAGTGACTCGACAAAATCGAAGGGCTCTTGG GGATCGGGGAAGGATCAGTATAGCAGGGAACTGCTTGTGTCCTCCATCTTCGCAGCCGCTAGTCGCAAGA GGAAAAAGCCCAAAGAGAAAGCCCAGCCCAGCAGCTCGGAAGACGAGCTGGACAGTGTGTTCTTTAAGAA GGAGAACATGGAACCGAGTCACAGTGAGATTAAAGAAGAGTCCAAAAGAGAAAGTGAGACACCAGGTAGA AAGCAGAGGGTTGTCGTTGCCAAAGACAGTAACACTAAGAAAGACACGGGTACAACGAAGGAGGAGAAGA AGATGCCATCGGACGAACCATCCCCACCACACAGCTCCAAACGCAACAGGTCACCAACGCTCAGCTGTCG CCTGGCCATGCTGAAGGAGAGCCCCAGATCACTTCTGACACAGAAGCCCCACTGTGAAGAGACAGGGTCT GACTCGGGCACTCTGTCTTCCCAGGCTTCACTAGCCAGGTCCTCCACTAAAAAATCCACCAGTCCAGAAA CCAGGCACACTGAGTTTTTGTCCAATGCTGGTACCATCACCTCAGACTATTCTACCACATCATCCACTAC CTACTTGACCAGCCTGGACTCCAGCCGCCTGAGCCCTGAGGTGCAGTCAGTGGCAGAGAGCAAGGGCGAC GAGGCTGATGACGAGAGGAGCGAACTGGTGAGTGAGGGGCGGCCCGTGGAGACGGACAGTGAGAGCGAGT TCCCGGTGTTCCCCTCCGCCCTGACTTCAGGGCAGTTCAGAGGAAAGCTACAGGAGGTCGCCAGGTCGAG CCGCAGAAACTCGGAAGGCAGTGAAGCCAGCTGCACCGAGGGAAGCCTCACACCAAGCTTAGACAGCCGC AGACAGCAGTTCAGCTCCCACAGGCTGATCGAATGCGACACGCTGTCCAGGAAGAAGTCTGCTCGCTTCA AGTCGGACAGTGGGAGCCCCGGAGACACCAGGACGGAGAAAGAAACGCCTGCCCTGGCCAAGATGTTTGA TGTCATGAAGAAAGGCAAGTCGACGGGGAGCCTCTTGACACCCAGCAGAAGTGAATCCGAAAAGCAGGAG GCCACTTGGAAAACAAAAATAGCAGATCGCTTAAAACTGAGACCCAGGGCACCCGCAGATGACATGTTTG GAGTTGGGAATCAAAAACCTACTGCCGAGACAGCAAAAAGGAAAAACATCAAACGCAGACACACGCTCGG AGGCCACAGAGATGCTACAGAAATCAGCGTCCTGAGCTTCTGGAAAGCGCAAGAGCAGAGTGCGGACAAA GAGTCCGAACTTTCAGCTGTGAACCGGTTAAAACCCAAATGCTCAGCCCAGGACCTCTCCATCTCAGACT GGCTGGCCAGGGAGCGCATCCGCTCCAGCGCCTCCGACCTCAGCCGAAGCGAAGGCTGCGAGCCCCAGGC CGAGAGCCCCAGCGTGCTGGGAACACCCACCAGCACCCACACACCGCCGCCCTCTCAGCAGCCTGAAGCT AGGGCTGCTGCCAGCACCTCGGCTTCAACCAGCCAGCCCCCTCTTTCCACACCACCACAGTCACCTGACC AAATAAACGGAGACAGCTTCCAGAACACGAGCCAAAATGCTAGTTCTGCGGCCAACGCCCACCCTCACAA ACTGTCTGAAAGTCCCAACACTAAAACAGAGTCTCCTCCCTGCCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217714 representing NM_001191693 Red=Cloning site Green=Tags(s) MMATHWTGLPEGDGDKLKACGAACEVSKNKDGKEQGEPVSPSEDESFSWPGPKTVMLKRTSQGFGFTLRH FIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKT YSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNGAYSGNARDIPEPPPVCYPW LPATPSATAQPVEVSPPDSLLNKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCSESVRTVIV PSEKVVDLLANRNSPSGPAHRTEEVRYGVSDQASAKAAARTTPPPSAPTARLSHQTIGSRSLEPSGILLK SGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRA ASQSAADYNQVVPARTALQVRRRSTSHDRVPQSIQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVS FSNHRTRSWDYIEGQSEATATVNSESQTPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSN LTVAPVVNSDNRRLGGRGVGPVSQFKKIPPDLRPPHSNRNFPTTSGMSLPRGIAQDRSPLVKVRSNSLKA LPPPVPKPSFSQHSLASTKDQRPVNHLHQHGLLSQQTRGRPESTFPHLELQTDVSPSLPGTSAKTSPQLS ENLGTSDLDLHASPRNGDVDSQEADVQPSDVPDNKESVILREKPQPGRQTPQPLRHQSYILAVNDQETGS DPTCWLPNDARREVHIKRMEERKASCTSPPGDSLASIPFIDEPTSPSIDHEVAHIPASAVISASTSQVPS IATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHTSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIK IADSQKSSEDSGSRKDSSSEVFSDAAKEGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRR EQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTG VTNRDLISRRIKEYNSLLSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPP KDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPG NNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLR TLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKI VETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSTKSKGSW GSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENMEPSHSEIKEESKRESETPGR KQRVVVAKDSNTKKDTGTTKEEKKMPSDEPSPPHSSKRNRSPTLSCRLAMLKESPRSLLTQKPHCEETGS DSGTLSSQASLARSSTKKSTSPETRHTEFLSNAGTITSDYSTTSSTTYLTSLDSSRLSPEVQSVAESKGD EADDERSELVSEGRPVETDSESEFPVFPSALTSGQFRGKLQEVARSSRRNSEGSEASCTEGSLTPSLDSR RQQFSSHRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSESEKQE ATWKTKIADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAQEQSADK ESELSAVNRLKPKCSAQDLSISDWLARERIRSSASDLSRSEGCEPQAESPSVLGTPTSTHTPPPSQQPEA RAAASTSASTSQPPLSTPPQSPDQINGDSFQNTSQNASSAANAHPHKLSESPNTKTESPPCL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191693
ORF Size	5856 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191693.1, NP_001178622.1
RefSeq Size	6640 bp
RefSeq ORF	5859 bp
Locus ID	307178
MW	215.6 kDa

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