Ubr1 (NM_001178072) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1562326
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217690 representing NM_001178072 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGAAGAGATGGACGGCGCCGAGAGGATGGACGTCAGCCCGGAGCCTCCCCTGGCCCCTCAGC CGCCCGCATCCTGGTGGGATCAGCAAGGTGATTTTTATACTGCTTTCTTACATCATTTGGCAAAATTAGT GCCAGAAATTTATTTTGCTGAGATGGACCCAGATTTGGAAAAGCAAGAAGAGAATGTACAAATGTCAATA TTCACTCCTTTGGAATGGTACTTATTTGGAGAGGATCCAGACGTTTGCTTAGAGAGATTAAAGCACAGTG CAGCATTTCAGTTGTGCGGGAAGGTTTTCAAAAGTGGAGAAACAACATATTCCTGTAGGGATTGTGCAAT TGATCCAACGTGTGTGCTCTGTATGGACTGCTTCCAGAGTAGTGTTCATAAAAACCATCGTTACAAGATG CATACTTCTACTGGAGGAGGCTTCTGTGACTGTGGAGACACAGAAGCGTGGAAAACTGGTCCCTTTTGTA TGGATCACGAACCTGGAAGAGCAGGTACTACAAAAGAGACCTTGCATTGCCCACTGAATGAAGAGGTAAT TGCTCAAGCCAGGAGAATATTCCCTTCAGTGATAAAGTACATTGTAGAAATGACTATATGGGAAGAGGAA AAGGAACTGCCTCCTGAACTGCAGAGAAGGGAGAAAAATGAAAGATATTACTGTGTCCTTTTCAACGATG AGCACCATTCATATGATCATGTGATATACAGTCTGCAAAGAGCTCTTGACTGTGAGCTTGCAGAGGCACA GTTGCACACAACTGCCATAGACAAAGAGGGTCGCCGGGCTGTCAAAGCAGGTGTGTATTCCGCTTGCCAG GAAGCAAAGGAGGATATAAAGAGTCACTCAGAGAACGTCTCTCAGCACCCCCTCCATGTGGAAGTGCTGC ATTCCGTGGTTATGGCCCACCAGAAATTTGCTCTGCGCCTTGGATCCTGGATGAACAAAATTATGAGCTA TTCAAGTGACTTTAGGCAGATCTTCTGCCAAGCCTGCCTCATAGAGGAGCCTGGCTCTGAACATCCCTGT CTTATAAGCAGACTAATGCTTTGGGATGCAAAACTTTATAAAGGTGCCCGTAAGATCCTTCACGAATTGA TCTTTAGTAGTTTTTTTATGGAGATGGAATACAAAAAACTCTTTGCTATGGAATTTGTGAAGTATTATAA ACAACTACAGAAAGAGTACATCAGTGACGACCACGAGAGAAGCATCTCTATAACCGCACTGTCCGTTCAG GTGTTCACTGTCCCCACCTTGGCCCGACATCTTATTGAAGAGCAGAATGTTATTTCTGTCATTACTGAAA CTCTGCTGGAAGTTCTACCTGAGTACTTGGACAGGAACAATAAGTTCAACTTCCAGGGCTATAGCCAGGA CAAGCTGGGAAGAGTGTACGCGGTGATCTGTGACCTGAAGTACATCCTGATTAGCAAGCCTCTCGTATGG ACAGAAAGACTAAGAGCACAGTTCCTGGAAGGGTTCCGGTCTTTTCTGAAGATTCTCACCTGTATGCAGG GAATGGAAGAAATCAGAAGACAAGTTGGACAGCACATTGAAGTAGACCCTGACTGGGAGGCTGCCATCGC TATACAGATGCAACTAAAGAATATTTTGCTCATGTTCCAAGAGTGGTGTGCTTGTGATGAAGATCTCTTA CTAGTGGCTTATAAAGAATGTCACAAAGCTGTAATGAGGTGCAGCACAAATTTCATGTCTAGTACCAAGA CAGTAGTACAACTGTGTGGTCATAGTTTGGAAACTAAATCCTACAAAGTGTCTGAGGACCTTGTCAGCAT ACATCTACCACTCTCTAGAACTCTCGCTGGTCTTCATGTACGTTTAAGCAGACTAGGTGCTGTTTCAAGA CTGCATGAATTTGTGCCTTTTGATGGCTTTCAAGTAGAGGTCCTAGTAGAGTACCCTTTGCGCTGCCTGG TGCTGGTTGCTCAGGTTGTTGCCGAGATGTGGCGAAGAAACGGGCTCTCCCTCATTAGCCAGGTTTTCTA TTATCAAGATGTTAAATGCAGGGAAGAAATGTATGATAAAGATATCATCATGCTTCAGATTGGAGCGTCT ATAATGGATCCCAACAAGTTCTTGTTACTGGTACTTCAGAGGTATGAACTTGCTGATGCTTTTAACAAGA CCATATCCACAAAAGACCAGGATTTGATTAAACAGTATAACACATTAATAGAAGAAATGCTTCAGGTCCT CATCTATATTGTGGGAGAACGTTATGTACCCGGAGTGGGAAACGTGACCAAAGAGGAGGTTATAATGAGA GAGATCACTCACTTACTTTGCATTGAGCCCATGCCACACAGTGCCATTGCCAGAAACCTACCGGAGAATG AAAATAACGAAACTGGCTTAGAGAATGTCATAAACAAAGTGGCCACATTTAAGAAACCGGGTGTGTCGGG CCATGGTGTTTATGAATTGAAAGACGAATCACTGAAAGACTTCAATATGTACTTTTACCATTATTCTAAA ACACAGCATAGCAAGGCTGAACATATGCAGAAGAAGAGGAGAAAACAAGAAAATAAAGATGAAGCCTTGC CACCGCCACCTCCTCCTGAGTTCTGTCCCGCTTTCAGCAAAGTAACTGACCTTCTCAACTGTGATATTAT GATGCACATTCTCAGGACCATCTTCGAGCGGGCAGTGGACCCCGAGTCTAATCTGTGGACAGAAGGGATG CTCCAAATGGCCTTCCATATATTGGCGCTGGGCTTGCTGGAGGAGAAGCAGCAGCTTCAGAAAGCTCCTG AAGAAGAAGTGGCTTTCGACTTTTACCATAAGGCTTCAAGATTGGGAAGTTCAGCAATGAATGCTCAGAA TATACAAATGCTTTTGGAAAAACTCAAAGGAATCCCCCAATTAGAAGGCCAGAAGGACATGATAACATGG ATTCTCCAGATGTTTGACACAGTGAAGCGATTAAGAGAAAAATCTTGTTTAGTTGTGGCAAGCACTTCAG GATTGGAGTCCATTAAAAGTGAGGAGATTACTCATGATAAAGAAAAAGCAGAACGGAAGAGAAAAGCTGA AGCTGCTAGGCTACATCGCCAGAAGATCATGGCCCAGATGTCTGCCTTACAGAAAAACTTCATTGAAACT CACAAACTCATGTATGACAATACATCAGAAATAACAGGGAAGGAAGACTCCATTATGGAAGAAGAAAGCC CCTCAGCAGTCAGTGAGTGCTCTCGAATTGCTCTGGGCCCTAAACGGGGCCCAGCTCTTACAGAAAAGGA GGTGCTGACGTGCATCCTCTGCCAAGAAGAACAAGAGGTGAAACTAGAAAACAACGCCATGGTGTTGTCC GCCTGTGTGCAGAAATCCACCGCCCTAACCCAGCACAGGGGGAAGCCTGCGGGCCTCTTAGGGGAATCAC TGGACCCTCTTTTCATGGATCCAGACCTGGCACATGGCACTTACGCAGGAAGCTGTGGTCATGTAATGCA TGCAGTGTGCTGGCAGAAGTATTTTGAAGCAGTGCAGCTGAGCTCTCAGCAGCGCATTCACGTCGACCTG TTTGACTTGGAGAGTGGAGAGTACCTGTGCCCTCTCTGCAAGTCTCTCTGCAATACCGTCATCCCCATCA TTCCTTTGCAGCCACAGAAGATCAACAGTGAGAATGCAGAGGCTCTTTCTCAACTTTTGACCTTGGCACG GTGGATACAGACTGTCCTTGCAAGAATATCAGGTTATAATATGAAACACGCTAAAGGAGAAGCTCCAGCA GTTCCTGTCCTGTTTAATCAAGGAATGGGGGATTCTACTTTTGAGTTTCATTCCATCCTGAGTTTTGGAG TTCAGTCTTCGGTGAAATACTCAAATAGTATCAAGGAAATGGTCATTCTCTTTGCCACAACAATTTACAG AATCGGCCTGAAAGTGCCTCCTGATGAATTAGACCCACGAGTACCCATGATGACCTGGAGCACGTGTGCT TTCACCATCCAGGCAATTGAAAACCTTTTGGGAGATGAAGGAAAACCTCTATTTGGAGCACTTCAAAATA GACAGCATAATGGTCTGAAAGCGTTAATGCAATTTGCAGTTGCACAGAGGACTACCTGTCCTCAGGTCCT GATACAGAAACATCTGGTTCGACTCCTATCAGTTGTTCTTCCTAACCTACAATCAGAAAATACACCATGC CTTCTATCCGTTGATCTATTTCATGTTCTGGTGGGCGCTGTCTTAGCGTTCCCGTCCTTGTACTGGGATG ACACGGTGGATCTGCAGCCTTCACCGCTCAGCTCTTCATACAGCCACCTTTATCTCTTCCATCTGGTCAC CATGGCCCATATGCTTCAGATACTCCTCACGGTAGATGCAGATCAGTCTCCGGGACTCCCACTTAATGAG GGTGGAGAGGACAGTGAAGAGGCTCGCTGTGCGTCTGCTCTCTTCGTGGAAGTCTCACAGCACACAGACG GCCTCATTGGGTGTGGTGCTCCTGGCTGGTACCTGTGGCTCTCCCTGAGGAATGGCATCACCCCTTACCT CCGCTGTGCTGCACTGCTTTTCCACTATTTACTCGGAGTAACTCCGCCTGAAGAACTGTTTGCCAACTCT GCTGAAGGAGAATTCAGTGCACTCTGTAGCTATCTCTCTTTACCCACAAATTTGTTCCTGCTTTTCCAGG AATATTGGGATACCATAAGGCCCTTACTTCAGAGGTGGTGTGGAGATCCTGCCTTACTCAACTCTTTGAA GCAGAAAAGTGCTCTGGTCAGGTACCCTAGAAAAAGAAATAGTTTGATAGAGCTTCCTGATGACTACAGC TGTCTTCTAAATCAAGCTTCTCACTTTAGGTGTCCACGGTCTTCAGATGATGAACGAAAGCACCCTGTCC TCTGTCTTTTCTGTGGGGCCATCCTGTGTTCTCAGAACATCTGTTGCCAAGAAATCGTGAATGGGGAGGA GGTTGGAGCGTGCGTTTTTCATGCACTTCAGTGTGGTGCTGGAGTCTGCATTTTCCTAAAAATCAGAGAA TGCAGAGTGGTCCTGGTAGAAGGAAAAGCCAGAGGCTGTGCCTACCCAGCCCCTTACTTGGATGAATATG GAGAAACAGACCCAGGGCTAAAGAGGGGAAACCCACTTCATTTATCTCGGGAGCGGTATCGGAAGCTGCA TTTGGTCTGGCAACAGCACTGCATTATAGAAGAGATTGCTCGGAGCCAGGAGACTAATCAGATGCTGTTT GGATTCAACTGGCAGTTACTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217690 representing NM_001178072 Red=Cloning site Green=Tags(s) MADEEMDGAERMDVSPEPPLAPQPPASWWDQQGDFYTAFLHHLAKLVPEIYFAEMDPDLEKQEENVQMSI FTPLEWYLFGEDPDVCLERLKHSAAFQLCGKVFKSGETTYSCRDCAIDPTCVLCMDCFQSSVHKNHRYKM HTSTGGGFCDCGDTEAWKTGPFCMDHEPGRAGTTKETLHCPLNEEVIAQARRIFPSVIKYIVEMTIWEEE KELPPELQRREKNERYYCVLFNDEHHSYDHVIYSLQRALDCELAEAQLHTTAIDKEGRRAVKAGVYSACQ EAKEDIKSHSENVSQHPLHVEVLHSVVMAHQKFALRLGSWMNKIMSYSSDFRQIFCQACLIEEPGSEHPC LISRLMLWDAKLYKGARKILHELIFSSFFMEMEYKKLFAMEFVKYYKQLQKEYISDDHERSISITALSVQ VFTVPTLARHLIEEQNVISVITETLLEVLPEYLDRNNKFNFQGYSQDKLGRVYAVICDLKYILISKPLVW TERLRAQFLEGFRSFLKILTCMQGMEEIRRQVGQHIEVDPDWEAAIAIQMQLKNILLMFQEWCACDEDLL LVAYKECHKAVMRCSTNFMSSTKTVVQLCGHSLETKSYKVSEDLVSIHLPLSRTLAGLHVRLSRLGAVSR LHEFVPFDGFQVEVLVEYPLRCLVLVAQVVAEMWRRNGLSLISQVFYYQDVKCREEMYDKDIIMLQIGAS IMDPNKFLLLVLQRYELADAFNKTISTKDQDLIKQYNTLIEEMLQVLIYIVGERYVPGVGNVTKEEVIMR EITHLLCIEPMPHSAIARNLPENENNETGLENVINKVATFKKPGVSGHGVYELKDESLKDFNMYFYHYSK TQHSKAEHMQKKRRKQENKDEALPPPPPPEFCPAFSKVTDLLNCDIMMHILRTIFERAVDPESNLWTEGM LQMAFHILALGLLEEKQQLQKAPEEEVAFDFYHKASRLGSSAMNAQNIQMLLEKLKGIPQLEGQKDMITW ILQMFDTVKRLREKSCLVVASTSGLESIKSEEITHDKEKAERKRKAEAARLHRQKIMAQMSALQKNFIET HKLMYDNTSEITGKEDSIMEEESPSAVSECSRIALGPKRGPALTEKEVLTCILCQEEQEVKLENNAMVLS ACVQKSTALTQHRGKPAGLLGESLDPLFMDPDLAHGTYAGSCGHVMHAVCWQKYFEAVQLSSQQRIHVDL FDLESGEYLCPLCKSLCNTVIPIIPLQPQKINSENAEALSQLLTLARWIQTVLARISGYNMKHAKGEAPA VPVLFNQGMGDSTFEFHSILSFGVQSSVKYSNSIKEMVILFATTIYRIGLKVPPDELDPRVPMMTWSTCA FTIQAIENLLGDEGKPLFGALQNRQHNGLKALMQFAVAQRTTCPQVLIQKHLVRLLSVVLPNLQSENTPC LLSVDLFHVLVGAVLAFPSLYWDDTVDLQPSPLSSSYSHLYLFHLVTMAHMLQILLTVDADQSPGLPLNE GGEDSEEARCASALFVEVSQHTDGLIGCGAPGWYLWLSLRNGITPYLRCAALLFHYLLGVTPPEELFANS AEGEFSALCSYLSLPTNLFLLFQEYWDTIRPLLQRWCGDPALLNSLKQKSALVRYPRKRNSLIELPDDYS CLLNQASHFRCPRSSDDERKHPVLCLFCGAILCSQNICCQEIVNGEEVGACVFHALQCGAGVCIFLKIRE CRVVLVEGKARGCAYPAPYLDEYGETDPGLKRGNPLHLSRERYRKLHLVWQQHCIIEEIARSQETNQMLF GFNWQLL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001178072
ORF Size	5271 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001178072.1, NP_001171543.1
RefSeq Size	5274 bp
RefSeq ORF	5274 bp
Locus ID	499877
MW	200.2 kDa

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