Zc3h13 (NM_001170471) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1307729
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217687 representing NM_001170471 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAAAAATTAGGAGGAAGGTCACAGTGGAAAACACCAAGACCATATCTGAAAGCACATCCCGAAGAC CTAGTGTGTTTGAGAGGCTTGGACCCAGCACTGGAAGCACAACAGAGACACAGTGCCGTAACTGGCTGAA GACCGGCAGCTGCCTCTATGGAAACACATGTAGGTTCATACATGGCCCTTCACCTCGTGGGAAAGGTTAC AGCAGCAATTACAGAAGGTCACCGGAAAGGCCTACAGGTGATCTCAGAGAAAGAATGAAGAATAAGCGTC AGGATGTGGATTCTGAATCCCAGAAACGGAACACAGAGGAGTCATCCTCACCTGTTAGGAAAGAATCTTC AAGAGGGAGACATAGAGACAAGGAAGACATAAAAATTGTTAAAGAGAGAACTCCAGAAAGCGAAGAAGAA AACGTAGAATGGGAAACCAACAGAGATGATTCTGACAATGGAGATATTAATTACGATTATGTTCATGAGT TATCATTGGAAATGAAGCGTCAGAAAATACAGAGAGAATTAATGAAGTTGGAACAAGAAAACATGGATAA AAGAGAAGAAATTATTATTCAAAAGGAGGTTTCCCCAGAAGTGGTGAGATCAAAGTTGTCTCCATCACCT CTGAGAAAGTCGAGTAAATCTCCAAAGCGGAAGTCCAGCCCCAAGGCATCTTCAGCTGGCAAGAAAGAGC GGAAGGCTGCTGTGGTGGCTTCCCCGCTGCTGGACCAGCAGAGAAATTCAAAAAGCAACCAAAGTAAAAA GAAAGGGCCACGCACTCCTAGTCCACCTCCTCCTATACTAGAAGATATCATTCTGGGAAAAAAATACAAA GAAAAGTATAAAGTGAAAGACAAGATAGAAGAAAAACCAAGAGATGGAAAGGACAGAGGACGGGATTTTG AAAGGCAAAGAGAAAAACGAGACAAACCAAGGTCTTCCTCCCCGGGACAGCACCATTCTCCTCTGTCTTC TAGACATCACTCGTCGTCCTCACAGTCAGGGTCATCTATTCAGAGACATTCTCCCTCTCCTCGCCGGAAA AGAACTCCTTCCCCATCCTACCAGAGGACTTTAACTCCATCTTTACGACGTTCTGCATCTCCTTATCCAA CCCATTGTCTGTCTTCTCCTCAGAGAAAGCAAAGTCCTCCAAGACATCGCTCTCCAATGAGAGAGAAAGG GAGGCATGATCATGAACGGACTTCACAGTCACATGATCGGCGTCATGAAAGGAGGGAAGAAGCTCGAGGC AAAAGGGATAGAGAAAAGGACACCAGAGAAGAGCGAGAGTCTGAACATGACCACAGGGATGACAGAGAAC CGCGAGACAGTCGGGACCGGAGAGACACTAGAGACCGCAGGGAACTTAGAGACTCCAGAGACATGCGGGA CTCCAGAGAGATAAGGGATTATAGCAGAGACACCAAGGAGAGCCGTGATCCCAGAGATTCCCGGTCAGCT CGGGATGTCCATGACTACAGGGACCGTGAAGCTCGGGATGCTCATGCTCGAGATGTTCGGGATGTCAGGG ATGCTCGAGATGCTCGAGATGTTCGGGATGTCCGGGATGTCAGGGATGCTCGAGATGTCCGGGATGTTCG GGATGCTCGAGATGTCCGGGATGCCCGGGATGTCCGGGATGTTCGGGATGTCCGGGATGTCCGGGACACC AGAGATGCCCGGGATGCCCGGGATGCTCGAGATGGTCATCGAAAGGAGGATGTATACCAGGAAGAAGCCC GTAGTTATGGCAGAAACCACCTGAGAGAAGAGAGTTCTAGAGTTGAACTAAGGAATGACTCTAGAAATGA GTCTCGAAGTGAAATTAGAAATGACCGCATGGGCAGAAGTAGGGGGAGAGGTCCAGAACTGCCTGAAAAG GGAGGTCGAAGCACAAGAGGTTCCCAAATGGATAGCCACAGTAGTGGCAGCAACTACCATGACAGCTGGG AAACTCGAAGTAGCTATCCTGAAAGAGACAGATACCCTGAGAGAGATACCAGAGATCAAGCAAGGGATTC TTCCTTTGAGAGAAGGCATGGAGAAAGAGACCGTCGGGATAACAGAGAACGAGATCAAAGACCAAGCTCA CCTATTCGCCATCAGGGGAGGAGTGAGGAGCTTGAGCGAGAGGAAAGAAGAGAGGAAAGACGAATAGACA GAGTGGATGAGAGACGAGATGAAAGGGTCAGAGACAGAGATAGGGAGAGAGAGAGAGAGCGGGAGAGGGA GAGAGATCGGGAACGGGAGCGGGAGCGGGAAAAGGAAAGAGAAAGAGAACTAGAAAGAGAGAGAGCTAGA GAGCGGGAGAGAGAGAGAGAGAAAGAGAGGGAGAGAGAGCGAGAAAGAGAAAGAGATCAGAGAGATCGTG ACCATGATCGAGAGCGAGAAAGAGAGAGGGAGCGAGAGAGGGAAAAAGAACGGGAGCGAGAAGAGAGAGA GAGGGAGAGAGAAAGGGAAAGGGAACGGGAAAGAGAAAGGGAACGGGAGCGGGAGAGAGAGCGAGAACGT GAGCGGGAGCGAGAAAGGGCCAGAGAAAGAGAAAAAGAACGAGAGCGGCAAAGAGAATGGGAGGATAAAG ACAAGGGACGCGATGACCGGAGGGAGAAGCGCGAGGACGTTCATGTTCGAGAGGACAGGATCCCCAGAGA CGGCCACGAGGAGAGAAAGTCCAAGAAACGCTATAGAAATGAAGGGAGTCCCAGCCCCCGGCAGTCCCCC AAGCGCCGGCGGGAGCATTCTCCTGACAGTGACACCTACCACAGTGGGGATGACAAAAATGAAAAGCATA GACTCTTGAGCCAGGTCGTACGGCCACAGGAATCTCGTTCTCTCAGCCCGTCCCACCTCACAGAAGACAG GCAGGGTAGATGGAAAGAGGAGGAGCGTAAATCGGAAAGGAAGGAGAGTTCAAGGCGCTATGAAGAACAA GAACTCAAAGAGAAGCTTTCTTGTGGCGACAGGCAAAGGGAGCAGACAGAAAGCGTGGACGGCGGCTCGA GAGCGCGCAGCGCGCAAGACCTGCTCAGCCATCGCCAGGCTGAAGACCGCGACCGAGATGGGTCTGATCG AGCCCATGATGAAAAGAAAAAAGCAAAAGCTCCGAAGAAACCCGTAAAGAAAAAGAGGGAGGAGGATGTT GGAATAGAGAGGGGCAACCCTGAGACACACGAGGATAGTCAAGTGTTTTCACCAAAAAAAGGACAGAAAA AGAAAAACATTGAAAAAAAGCGTAAAAGATCCAAGGGGGATTCTGATGTTTCTGACGAAGAGGCAGCCCC GCAGAGTAAGAAGAAGAGGGGTCCACGCACACCCCCTCTTGCCATCAAGGAGGAGCTGATTGAGATTTCC ACTGACAAGGAAGGCATCCTTGAGGATCCCCTGAAGAAAGAAGATACCGCCTTTAGTGACTGGTCTGATG AGGATGTGCCTGACCGCACAGACGGCTTGGAAGCTGAGCACACTGCAGCCGCGGCCACTCCCGGGAGCAC CCCTTCCCCCATGTCGTCTCTTCTCCCTCCCCCGCCCCCTGTGGCTGCTGCCGGTACTGCAGCCACTGCT CTTGCCGCCTCAGCCATTTCTGCCACCACCTCTACCACCATTTCAAGCTCTGGTGCCACCTCCAATATCA ATGCCAGTGAAGACTCACACAGAAAATGCCACAGGGCACGAGGGGAGAAGGTAGAGGTGTCTCATGTGAC CCTGGAAGATACACCACATCGCAAACTGGTGGATCAAAAGAGAAGCAGCAGCCTGGGAAGCAATCGGAGT CACCGCAGCCATACCTCTGGTCGCCTGCGCTCCCCATCTAATGATTCTGCACATCGAAGTGGGGACGATC AGGGTGGTCGGAAGAGAGTGCTCCACAGTGGCTCCAGAGATAGAGAAAAGACAAAGAGCCTAGAGCTCAC AGGAGAGAGAAAATCTAGGATCGATCAGTTAAAGCGTGGAGAGCCCAGTCGGAGTACTTCTTCAGATCGC CAGGATTCCAGAAGCCACAGCTCCAGAAGAAGCTCTCCTGAGTCAGACCGACAGGTTCACTCCAGGTCTG GGTCATTTGACAGCAGAGACCGGCTTCAAGAACGAGATAGGTATGAGCATGACAGAGAGCGTGAGAGGGA GCGGAGAGATCCCAGACAAAGGGAGTGGGACCGGGAAGCAGACAAAGAGTGGCCTCGGACCCGAGACCGT GATAGACTTCGGGAACGCGAGAGAGACCGAGACAAAAGGAGAGACTTGGACAGAGAGAGAGAGAGACTGG TCGCCGACCCCATGGAAAGAGACAGAGAACGAGATAGAACTTTTGAGACGTCTCAGTTGGAATCTGTGAA GCGAAGTGAAGTCAAATTGGAGAGTGAACACGACAGAGACCTGGAGGGCAGCTCCCGGGACTCTGTGGCC TTGGACAAAGAAAGGATGGACAGAGACCTGGGGTCTGCGCAGGGGTTTGAGGATGTGAGTAAAGCGGAGA GAACAGAGAGTCTGGAAGCAGGAGATGACGAGTCCAAGTTAGATGATGCGCATTCGTTAGGGTCTGGTGC TGGGGAAGGCTATGAGCCCATCAGTGACGACGAGCTAGATGAAATTCTGGCAGGGGATGCAGAAAAGAGA GAGGACCAGCAGGAAGAGGAGAAGATGCCAGATCCATTAGATGTGATAGATGTGGACTGGTCTGGACTAA TGCCAAAGCATCGGAAAGAGCCGAGAGAGCCCGGGGCTGCACTCTTAAAGTTCACACCCGGAGCCGTGCT ACTACGAGTTGGGATTTCTAAAAAGTTGGCCGGTTCTGAGCTCTTTACAAAAGTTAAAGAAACATGTCAA CAACTTCTAGAGAAACCCAAAGATGCAGACAGCCTTTTTGAACATGAACTAGGGGCTCTCAACATGGCTG CTTTACTTCGAAAAGAAGAGCGGGCGAGTCTCCTTAGTGACCTGGGACCATGTTGTAAAGCATTATGTTT CAGACGGGATTCTGCAATCCGGAAGCAGCTTGTGAAGAACGAAAAGGGAACCGTGAAGCAGGCGTACACC AACACTCCGATGGTGGACAACGAGCTGCTTCGGCTGAGTCTTCGGTTACTCAAGAGGAAGACTTCCTGCC ACACCCCTGGGCAGGAAAAGACTGAAGATGGGAAATTAGCCCCGTGCAGTGGCCAGCAGGAGCTGTGTGT GTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217687 representing NM_001170471 Red=Cloning site Green=Tags(s) MSKIRRKVTVENTKTISESTSRRPSVFERLGPSTGSTTETQCRNWLKTGSCLYGNTCRFIHGPSPRGKGY SSNYRRSPERPTGDLRERMKNKRQDVDSESQKRNTEESSSPVRKESSRGRHRDKEDIKIVKERTPESEEE NVEWETNRDDSDNGDINYDYVHELSLEMKRQKIQRELMKLEQENMDKREEIIIQKEVSPEVVRSKLSPSP LRKSSKSPKRKSSPKASSAGKKERKAAVVASPLLDQQRNSKSNQSKKKGPRTPSPPPPILEDIILGKKYK EKYKVKDKIEEKPRDGKDRGRDFERQREKRDKPRSSSPGQHHSPLSSRHHSSSSQSGSSIQRHSPSPRRK RTPSPSYQRTLTPSLRRSASPYPTHCLSSPQRKQSPPRHRSPMREKGRHDHERTSQSHDRRHERREEARG KRDREKDTREERESEHDHRDDREPRDSRDRRDTRDRRELRDSRDMRDSREIRDYSRDTKESRDPRDSRSA RDVHDYRDREARDAHARDVRDVRDARDARDVRDVRDVRDARDVRDVRDARDVRDARDVRDVRDVRDVRDT RDARDARDARDGHRKEDVYQEEARSYGRNHLREESSRVELRNDSRNESRSEIRNDRMGRSRGRGPELPEK GGRSTRGSQMDSHSSGSNYHDSWETRSSYPERDRYPERDTRDQARDSSFERRHGERDRRDNRERDQRPSS PIRHQGRSEELEREERREERRIDRVDERRDERVRDRDRERERERERERDREREREREKERERELERERAR EREREREKERERERERERDQRDRDHDREREREREREREKEREREERERERERERERERERERERERERER ERERERAREREKERERQREWEDKDKGRDDRREKREDVHVREDRIPRDGHEERKSKKRYRNEGSPSPRQSP KRRREHSPDSDTYHSGDDKNEKHRLLSQVVRPQESRSLSPSHLTEDRQGRWKEEERKSERKESSRRYEEQ ELKEKLSCGDRQREQTESVDGGSRARSAQDLLSHRQAEDRDRDGSDRAHDEKKKAKAPKKPVKKKREEDV GIERGNPETHEDSQVFSPKKGQKKKNIEKKRKRSKGDSDVSDEEAAPQSKKKRGPRTPPLAIKEELIEIS TDKEGILEDPLKKEDTAFSDWSDEDVPDRTDGLEAEHTAAAATPGSTPSPMSSLLPPPPPVAAAGTAATA LAASAISATTSTTISSSGATSNINASEDSHRKCHRARGEKVEVSHVTLEDTPHRKLVDQKRSSSLGSNRS HRSHTSGRLRSPSNDSAHRSGDDQGGRKRVLHSGSRDREKTKSLELTGERKSRIDQLKRGEPSRSTSSDR QDSRSHSSRRSSPESDRQVHSRSGSFDSRDRLQERDRYEHDRERERERRDPRQREWDREADKEWPRTRDR DRLRERERDRDKRRDLDRERERLVADPMERDRERDRTFETSQLESVKRSEVKLESEHDRDLEGSSRDSVA LDKERMDRDLGSAQGFEDVSKAERTESLEAGDDESKLDDAHSLGSGAGEGYEPISDDELDEILAGDAEKR EDQQEEEKMPDPLDVIDVDWSGLMPKHRKEPREPGAALLKFTPGAVLLRVGISKKLAGSELFTKVKETCQ QLLEKPKDADSLFEHELGALNMAALLRKEERASLLSDLGPCCKALCFRRDSAIRKQLVKNEKGTVKQAYT NTPMVDNELLRLSLRLLKRKTSCHTPGQEKTEDGKLAPCSGQQELCVS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001170471
ORF Size	5184 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001170471.1, NP_001163942.1
RefSeq Size	6199 bp
RefSeq ORF	5187 bp
Locus ID	305955
MW	203.5 kDa

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