Plch1 (NM_001191707) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Plcl3; RGD1561991
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217677 representing NM_001191707 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGTAATGCAGTCCGGGACACAGATGATCAAGCTGAAGCGTGGCACCAAAGGTCTCGTTCGCCTCT TTTACCTGGATGAACACCGGACCCGCCTTCGATGGCGACCCTCTAGGAAGAGTGAGAAGGCAAAAATACT TATTGATTCCATCTACAAAGTCACCGAGGGCAGGCAGTCTGAAATTTTCCACAGACAGGCTGAGGGGAAC TTTGATCCCAGCTGCTGCTTTACCATCTACCATGGCAACCACATGGAGTCCCTGGACCTCATTACCTCCA ACCCAGAGGAGGCACGCACCTGGATCACGGGCCTCAAGTATCTGATGGCTGGCATCAGTGATGAAGACTC CCTTGCCAAGAGGCAAAGGACCCATGACCAATGGGTGAAGCAGACCTTTGAGGAGGCTGATAAGAATGGT GATGGCTTATTGAATATTGAAGAGATTCACCAGCTGATGCATAAACTAAATGTCAATCTGCCTCGAAGAA AAGTCAGGCAAATGTTTCAGGAAGCGGACACAGATGAGAATCAGGGGACCTTGACATTTGAAGAGTTCTG CGTTTTTTACAAAATGATGTCATTGAGACGAGACCTCTATTTGCTGCTCTTGAGCTACAGTGACAAGAAA GACCACCTTACTGTGGAGGAGCTAGCTCAGTTCTTGAAGGTGGAACAAAAGATGAGTAATGTCACACTGG ACTATTGTCTGGACGTCATAAAGAAGTTCGAAGTTTCTGAAGAAAACAAAGTGAAAAACGTCCTCGGTAT AGAAGGTTTCACGAACTTCATGCGTAGCCCTGCTTGTGATGTATTTAACCCTTTGCACCATGAAGTATAC CAAGACATGGATCAGCCTCTCTGCAATTACTACATTGCTTCTTCTCATAACACATACCTGACTGGGGACC AGCTCCTTTCTCAATCCAAAGTGGACATGTATGCACGGGTGCTACAAGAGGGATGTCGATGCGTGGAAGT TGACTGTTGGGATGGCCCAGATGGAGAGCCAGTGGTCCACCATGGTTATACTCTCACTTCAAAAATTCTC TTCAAAGATGTTGTGGAGACCATCAACAAGCATGCATTTGTTAAAAATGAGTTTCCCGTTATACTGTCCA TTGAGAACCACTGCAGTATTCAGCAGCAGAGGAAGATTGCTCAGTACCTGAAAGGAATATTCCAGGACAA ACTGGACCTGTCATCTGTAGACACAGGAGAGTGCAGGCAGCTTCCAAGCCCTCAGAGTCTGAAAGGCAAA ATCCTGGTGAAGGGCAAGAAGTTGCCATATCACCTTGGGGATGATGCAGAGGAAGGAGAAGTGTCCGATG AGGACAGTGCAGATGAAATTGAAGATGAATGCAAGTTCAAGCTGCATTATAGTAACGGGACCACTGAGCA CCAGGTAGAATCTTTCATACGGAAAAAGCTGGACTCACTGTTGAAGGAGTCTCAGATTCGAGACAAGGAA GACCCAGACAGTTTCACAGTGAGGGCGTTACTCAAGGCTACACATGAAGGCTTAAATGCACACCTGAAGC AGAACCTGGATGTGAAGGAAAGTGGAAAGAAGTCCCATGGGCGATCCCTGATGGCCAACTTTGGGAAACA CAAGAAAACTCCAAAATCACGTTCTAAATCCTATAGTACTGATGATGAGGAAGATACACTACAGAATCCT GGCAAGGAGGGAGGCCAGCTGTACAGGCTGGGCCGCCGAAGGAGAACCATGAAACTCTGCAGAGAGCTCT CGGACTTGGTGGTGTACACAAACTCTGTGGCAGCCCAGGATATTGTGGATGATGGGACCACAGGGAATGT GTTGTCCTTCAGTGAAACAAGAGCACATCAAGTGGTTCAGCAGAAGTCAGAGCAGTTCATGATCTACAAC CAAAAACAGCTCACGAGGATTTACCCCTCTGCCTACCGCATCGATTCCAGTAACTTCAACCCTCTGCCCT ACTGGAATGCGGGCTGCCAGCTAGTGGCTCTGAACTACCAGTCTGAAGGGCGAATGATGCAGATAAATCG AGCAAAATTCAAAGCAAACGGCAACTGTGGCTACATTCTCAAGCCCCAGCAAATGTGCAAAGGAACATTC AACCCTTTGTCTGGTGACCCACTTCCTGCCAACCCCAAAAAACAGCTCATACTGAAAGTGATCAGTGGAC AGCAACTCCCCAAACCCCCTGACTCTATGTTTGGAGACCGAGGCGAGATCATTGACCCTTTTGTTGAAGT TGAAATTATTGGGCTTCCTGTAGACTGTTGCAAGGACCAAACTCGTGTGGTGGATGACAATGGATTTAAC CCTGTGTGGGAAGAAACCCTAACTTTTACAGTTCACATGCCAGAAATAGCTTTGGTTCGATTTCTTGTGT GGGATCATGATCCTATCGGAAGAGACTTTGTGGGACAACGGACCGTGACCTTCAGCAGCCTCGTGCCTGG CTACCGGCATGTCTACCTGGAAGGCCTCACAGAAGCGTCTATTTTCGTTCACATAACAATCAATGAGATC TTTGGAAAGAACAGACAGCTCCAAGGCTTGAAGGGACTGTTCAATAAGAATCCCAGGCACGGTTCTTCAG AAAATAACTCCCATTATGTTCGGAAGCGATCAATTGGAGATAGAATTCTGCGACGTACAGCCAGTGCTCC AGCCAAAGGCAGGAAAAAGAGCAAAGTGGGCTTCCAAGAAATGGTTGAGATAAAAGATTCTGTCTGTGAG GCTACAAGAGATCAAGATGGTGTCCTGAGGAGGACCACACGGAGTCTGCAACTGCGCCCTGTCTCTATGC CTGTTGACAAGAACCTTCTGGGGGCTTTGTCACTGCCTATCTCTGAAGCAGCAAAAAACACAGATGGAAA AGAAAACTATCTGGCAGAGGACAAGGATGATGGAAGAAAGGGGCCAGCAACTATAAAAGACCCACATTTT TCCAATTTCAACAAAAAGTTATCCTCCTCCTCCAGTGCCCTCCTCCACAAAGATGCCAAAAACCAAGGGC CAACAGCCAGTGTATCAAACCCAGAACAGTGTGGAGGACTAGGTTTAAAGAGTGGGAGAATCAAACCACA TATGACAAATGATTGCCAGGAAAACCGCAGTCCCCAAAACTTCCTCTCTCCAAGGAAGCATTTGGCACTT GATCCCGCAACCAAGGGACTGCAAGATCTGCATGGAATGAGAACTGATGAAAAGGAGCAGGAGCGTGCCA AGGGCTTTGTGGGAAGGAAAAGCACGCAGAGCGAAAGTGTTCTTTCTCAAAGTTCTCTGGAGGTGGAGAA TCTGGAAGGCAGCAAGGCCAAGGGCAGAGCTGCAACGTCCTTTTCTCTCTCCGATGTCTCTGCACTCTGT TCTGACATTCCTGATTTGCATTCAACTGCCATTCTGCAGGACACTGAAATTTCCGATCTCATTAATGATG TGACTTTAACAAATGCAAATGAGTCGGGCAGTTCCATCTCAGCACTGATCGGCCAGTTTGAAGAGAGCAA CCATCGGGCTAACGTTGCCGTGGTTTCTCGTCTCCTTAGCACCAGTGGGACATCAGAAGGTGTTGCTTTG CCAGTCGCGTTGGGCCTACAAACACCCATCAAGAATGGCTTTTCCCAGGGGAAGCACAAGGCCTCCTTCT TGTGCTCATCTCCTGAGTTGAACAAGCCCTCTAGTGCGGAAACCACCAAACTGGCAAATAACACAGTTGC CTGTGGAGTGATTGGCTCTCCCATCTCTACACCAAAGCCAGGTGATGACGCTTCTGATAAAGCCAAGACA AGGGTCACGGAAGACAACCTGCCTGATTCCCCTGATGCTTTTCCTGGCCAGTTTCCCCAAAGCCCCACCC ACGGAGAGGACCATAGGCAAGTGATGAGCAGCCCTGCTCTCTCCATGCCGTTGGCCATGGAAGATACAAC AGCTGACCCCGCTCTCTGTATAAATTCTGAGGAGAGCAGTCTTGTGGAAATTGATGGAGACTCTGAAAGC CTATCTCTAACAGCCTGTGACTATGGGGAAGAAGCTCTGAGTCAACTTGTTTCTCCACTAAAACTGCAGC AGAGCCAGGAGACGGTGGAACACATCCAGAAAGGCTTGAGCAATGGCTACTGTAAAGAGACTCTCCTCCC TTCTGAAATATTCAACAATATTCCAGGTGTCAAAAATCAGAGTATTTCTCATCTAACCTATCAGGGTGCT GGCTTTGTGTATAACCATTTCTCAAGTTCAGATGCAAAAACGAACCAAATCTGTGGGCCCCAGCAGCCTA GGGCTCCAGATATGCATGTCCCTACACCCACACCCTCGACACATGCCCCTTTGGCTGCTTTGAAACTGCC AAGCCCATGCAAATCCAAAAGTCTGGGGGACTTAACATCAGAGGACATTGCCTGCAATTTTGAAAGCAAA TACCAATGTATTAGTAGGAGTTTCGTGACAAACAGCATTAGAGACAAGAGCGTGACTATGAAGACAAAGT CATTCGAGCCTCTAGATGCCCTAACTGAACAGCTCCGGAAGCTGGTGTCCTTTGACCAAGAAGACAGCTG TCAAGGGTTATACTCAAAGCAGGATGTCAATCAGTGTCCCAGGGCCTTAGTCAGAAAGTTGTCCTCTAGA AGTCAGAGCAGAGTTCGCAACATTGCTAGTCGTGCCAAGGAGAAGCAGGAAGCTGGCAAGCAAAAAGCTA TGACTCAGAACCCCAGAGGAGGAGTGGTCCTTAGAAGTAAACCGCCAGCTCCTACTCTGGCTGTGAACCG TCACTCCACGGGCTCATACATTGCAAGCTACCTGAGGAACATGAAAGCTAGTGGCCTAGAAGGTCGCGGC ATCCCAGAGGGAGCATGCACAGCCCTTCGCTACGGCTACATGGACCAGTTCTGTTCAGACAATTCAGTTC TGCAGACTGAGCCAAGCAGTGAAGATAAACCCGAAATTTATTTTCTTTTGAGGCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217677 representing NM_001191707 Red=Cloning site Green=Tags(s) MSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGN FDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNG DGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKK DHLTVEELAQFLKVEQKMSNVTLDYCLDVIKKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVY QDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKIL FKDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFQDKLDLSSVDTGECRQLPSPQSLKGK ILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLDSLLKESQIRDKE DPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKKTPKSRSKSYSTDDEEDTLQNP GKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYN QKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTF NPLSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFN PVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEI FGKNRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVCE ATRDQDGVLRRTTRSLQLRPVSMPVDKNLLGALSLPISEAAKNTDGKENYLAEDKDDGRKGPATIKDPHF SNFNKKLSSSSSALLHKDAKNQGPTASVSNPEQCGGLGLKSGRIKPHMTNDCQENRSPQNFLSPRKHLAL DPATKGLQDLHGMRTDEKEQERAKGFVGRKSTQSESVLSQSSLEVENLEGSKAKGRAATSFSLSDVSALC SDIPDLHSTAILQDTEISDLINDVTLTNANESGSSISALIGQFEESNHRANVAVVSRLLSTSGTSEGVAL PVALGLQTPIKNGFSQGKHKASFLCSSPELNKPSSAETTKLANNTVACGVIGSPISTPKPGDDASDKAKT RVTEDNLPDSPDAFPGQFPQSPTHGEDHRQVMSSPALSMPLAMEDTTADPALCINSEESSLVEIDGDSES LSLTACDYGEEALSQLVSPLKLQQSQETVEHIQKGLSNGYCKETLLPSEIFNNIPGVKNQSISHLTYQGA GFVYNHFSSSDAKTNQICGPQQPRAPDMHVPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESK YQCISRSFVTNSIRDKSVTMKTKSFEPLDALTEQLRKLVSFDQEDSCQGLYSKQDVNQCPRALVRKLSSR SQSRVRNIASRAKEKQEAGKQKAMTQNPRGGVVLRSKPPAPTLAVNRHSTGSYIASYLRNMKASGLEGRG IPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLLRL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191707
ORF Size	4956 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191707.1, NP_001178636.1
RefSeq Size	5623 bp
RefSeq ORF	4959 bp
Locus ID	310463
MW	184.2 kDa

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