Plch1 (NM_001191707) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Plcl3; RGD1561991
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217677 representing NM_001191707 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGTAATGCAGTCCGGGACACAGATGATCAAGCTGAAGCGTGGCACCAAAGGTCTCGTTCGCCTCT TTTACCTGGATGAACACCGGACCCGCCTTCGATGGCGACCCTCTAGGAAGAGTGAGAAGGCAAAAATACT TATTGATTCCATCTACAAAGTCACCGAGGGCAGGCAGTCTGAAATTTTCCACAGACAGGCTGAGGGGAAC TTTGATCCCAGCTGCTGCTTTACCATCTACCATGGCAACCACATGGAGTCCCTGGACCTCATTACCTCCA ACCCAGAGGAGGCACGCACCTGGATCACGGGCCTCAAGTATCTGATGGCTGGCATCAGTGATGAAGACTC CCTTGCCAAGAGGCAAAGGACCCATGACCAATGGGTGAAGCAGACCTTTGAGGAGGCTGATAAGAATGGT GATGGCTTATTGAATATTGAAGAGATTCACCAGCTGATGCATAAACTAAATGTCAATCTGCCTCGAAGAA AAGTCAGGCAAATGTTTCAGGAAGCGGACACAGATGAGAATCAGGGGACCTTGACATTTGAAGAGTTCTG CGTTTTTTACAAAATGATGTCATTGAGACGAGACCTCTATTTGCTGCTCTTGAGCTACAGTGACAAGAAA GACCACCTTACTGTGGAGGAGCTAGCTCAGTTCTTGAAGGTGGAACAAAAGATGAGTAATGTCACACTGG ACTATTGTCTGGACGTCATAAAGAAGTTCGAAGTTTCTGAAGAAAACAAAGTGAAAAACGTCCTCGGTAT AGAAGGTTTCACGAACTTCATGCGTAGCCCTGCTTGTGATGTATTTAACCCTTTGCACCATGAAGTATAC CAAGACATGGATCAGCCTCTCTGCAATTACTACATTGCTTCTTCTCATAACACATACCTGACTGGGGACC AGCTCCTTTCTCAATCCAAAGTGGACATGTATGCACGGGTGCTACAAGAGGGATGTCGATGCGTGGAAGT TGACTGTTGGGATGGCCCAGATGGAGAGCCAGTGGTCCACCATGGTTATACTCTCACTTCAAAAATTCTC TTCAAAGATGTTGTGGAGACCATCAACAAGCATGCATTTGTTAAAAATGAGTTTCCCGTTATACTGTCCA TTGAGAACCACTGCAGTATTCAGCAGCAGAGGAAGATTGCTCAGTACCTGAAAGGAATATTCCAGGACAA ACTGGACCTGTCATCTGTAGACACAGGAGAGTGCAGGCAGCTTCCAAGCCCTCAGAGTCTGAAAGGCAAA ATCCTGGTGAAGGGCAAGAAGTTGCCATATCACCTTGGGGATGATGCAGAGGAAGGAGAAGTGTCCGATG AGGACAGTGCAGATGAAATTGAAGATGAATGCAAGTTCAAGCTGCATTATAGTAACGGGACCACTGAGCA CCAGGTAGAATCTTTCATACGGAAAAAGCTGGACTCACTGTTGAAGGAGTCTCAGATTCGAGACAAGGAA GACCCAGACAGTTTCACAGTGAGGGCGTTACTCAAGGCTACACATGAAGGCTTAAATGCACACCTGAAGC AGAACCTGGATGTGAAGGAAAGTGGAAAGAAGTCCCATGGGCGATCCCTGATGGCCAACTTTGGGAAACA CAAGAAAACTCCAAAATCACGTTCTAAATCCTATAGTACTGATGATGAGGAAGATACACTACAGAATCCT GGCAAGGAGGGAGGCCAGCTGTACAGGCTGGGCCGCCGAAGGAGAACCATGAAACTCTGCAGAGAGCTCT CGGACTTGGTGGTGTACACAAACTCTGTGGCAGCCCAGGATATTGTGGATGATGGGACCACAGGGAATGT GTTGTCCTTCAGTGAAACAAGAGCACATCAAGTGGTTCAGCAGAAGTCAGAGCAGTTCATGATCTACAAC CAAAAACAGCTCACGAGGATTTACCCCTCTGCCTACCGCATCGATTCCAGTAACTTCAACCCTCTGCCCT ACTGGAATGCGGGCTGCCAGCTAGTGGCTCTGAACTACCAGTCTGAAGGGCGAATGATGCAGATAAATCG AGCAAAATTCAAAGCAAACGGCAACTGTGGCTACATTCTCAAGCCCCAGCAAATGTGCAAAGGAACATTC AACCCTTTGTCTGGTGACCCACTTCCTGCCAACCCCAAAAAACAGCTCATACTGAAAGTGATCAGTGGAC AGCAACTCCCCAAACCCCCTGACTCTATGTTTGGAGACCGAGGCGAGATCATTGACCCTTTTGTTGAAGT TGAAATTATTGGGCTTCCTGTAGACTGTTGCAAGGACCAAACTCGTGTGGTGGATGACAATGGATTTAAC CCTGTGTGGGAAGAAACCCTAACTTTTACAGTTCACATGCCAGAAATAGCTTTGGTTCGATTTCTTGTGT GGGATCATGATCCTATCGGAAGAGACTTTGTGGGACAACGGACCGTGACCTTCAGCAGCCTCGTGCCTGG CTACCGGCATGTCTACCTGGAAGGCCTCACAGAAGCGTCTATTTTCGTTCACATAACAATCAATGAGATC TTTGGAAAGAACAGACAGCTCCAAGGCTTGAAGGGACTGTTCAATAAGAATCCCAGGCACGGTTCTTCAG AAAATAACTCCCATTATGTTCGGAAGCGATCAATTGGAGATAGAATTCTGCGACGTACAGCCAGTGCTCC AGCCAAAGGCAGGAAAAAGAGCAAAGTGGGCTTCCAAGAAATGGTTGAGATAAAAGATTCTGTCTGTGAG GCTACAAGAGATCAAGATGGTGTCCTGAGGAGGACCACACGGAGTCTGCAACTGCGCCCTGTCTCTATGC CTGTTGACAAGAACCTTCTGGGGGCTTTGTCACTGCCTATCTCTGAAGCAGCAAAAAACACAGATGGAAA AGAAAACTATCTGGCAGAGGACAAGGATGATGGAAGAAAGGGGCCAGCAACTATAAAAGACCCACATTTT TCCAATTTCAACAAAAAGTTATCCTCCTCCTCCAGTGCCCTCCTCCACAAAGATGCCAAAAACCAAGGGC CAACAGCCAGTGTATCAAACCCAGAACAGTGTGGAGGACTAGGTTTAAAGAGTGGGAGAATCAAACCACA TATGACAAATGATTGCCAGGAAAACCGCAGTCCCCAAAACTTCCTCTCTCCAAGGAAGCATTTGGCACTT GATCCCGCAACCAAGGGACTGCAAGATCTGCATGGAATGAGAACTGATGAAAAGGAGCAGGAGCGTGCCA AGGGCTTTGTGGGAAGGAAAAGCACGCAGAGCGAAAGTGTTCTTTCTCAAAGTTCTCTGGAGGTGGAGAA TCTGGAAGGCAGCAAGGCCAAGGGCAGAGCTGCAACGTCCTTTTCTCTCTCCGATGTCTCTGCACTCTGT TCTGACATTCCTGATTTGCATTCAACTGCCATTCTGCAGGACACTGAAATTTCCGATCTCATTAATGATG TGACTTTAACAAATGCAAATGAGTCGGGCAGTTCCATCTCAGCACTGATCGGCCAGTTTGAAGAGAGCAA CCATCGGGCTAACGTTGCCGTGGTTTCTCGTCTCCTTAGCACCAGTGGGACATCAGAAGGTGTTGCTTTG CCAGTCGCGTTGGGCCTACAAACACCCATCAAGAATGGCTTTTCCCAGGGGAAGCACAAGGCCTCCTTCT TGTGCTCATCTCCTGAGTTGAACAAGCCCTCTAGTGCGGAAACCACCAAACTGGCAAATAACACAGTTGC CTGTGGAGTGATTGGCTCTCCCATCTCTACACCAAAGCCAGGTGATGACGCTTCTGATAAAGCCAAGACA AGGGTCACGGAAGACAACCTGCCTGATTCCCCTGATGCTTTTCCTGGCCAGTTTCCCCAAAGCCCCACCC ACGGAGAGGACCATAGGCAAGTGATGAGCAGCCCTGCTCTCTCCATGCCGTTGGCCATGGAAGATACAAC AGCTGACCCCGCTCTCTGTATAAATTCTGAGGAGAGCAGTCTTGTGGAAATTGATGGAGACTCTGAAAGC CTATCTCTAACAGCCTGTGACTATGGGGAAGAAGCTCTGAGTCAACTTGTTTCTCCACTAAAACTGCAGC AGAGCCAGGAGACGGTGGAACACATCCAGAAAGGCTTGAGCAATGGCTACTGTAAAGAGACTCTCCTCCC TTCTGAAATATTCAACAATATTCCAGGTGTCAAAAATCAGAGTATTTCTCATCTAACCTATCAGGGTGCT GGCTTTGTGTATAACCATTTCTCAAGTTCAGATGCAAAAACGAACCAAATCTGTGGGCCCCAGCAGCCTA GGGCTCCAGATATGCATGTCCCTACACCCACACCCTCGACACATGCCCCTTTGGCTGCTTTGAAACTGCC AAGCCCATGCAAATCCAAAAGTCTGGGGGACTTAACATCAGAGGACATTGCCTGCAATTTTGAAAGCAAA TACCAATGTATTAGTAGGAGTTTCGTGACAAACAGCATTAGAGACAAGAGCGTGACTATGAAGACAAAGT CATTCGAGCCTCTAGATGCCCTAACTGAACAGCTCCGGAAGCTGGTGTCCTTTGACCAAGAAGACAGCTG TCAAGGGTTATACTCAAAGCAGGATGTCAATCAGTGTCCCAGGGCCTTAGTCAGAAAGTTGTCCTCTAGA AGTCAGAGCAGAGTTCGCAACATTGCTAGTCGTGCCAAGGAGAAGCAGGAAGCTGGCAAGCAAAAAGCTA TGACTCAGAACCCCAGAGGAGGAGTGGTCCTTAGAAGTAAACCGCCAGCTCCTACTCTGGCTGTGAACCG TCACTCCACGGGCTCATACATTGCAAGCTACCTGAGGAACATGAAAGCTAGTGGCCTAGAAGGTCGCGGC ATCCCAGAGGGAGCATGCACAGCCCTTCGCTACGGCTACATGGACCAGTTCTGTTCAGACAATTCAGTTC TGCAGACTGAGCCAAGCAGTGAAGATAAACCCGAAATTTATTTTCTTTTGAGGCTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191707
Insert Size	4959 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191707.1, NP_001178636.1
RefSeq Size	5623 bp
RefSeq ORF	4959 bp
Locus ID	310463

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