Drosha (NM_001107655) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Etohi2; RGD1307626; Rn3; Rnasen
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217604 representing NM_001107655 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAGGCAATACGTGTCATAGAATGTCTTACCACCCAGGACGAGGGTGTCCCCGGGGACGAGGAGGAC ATGGAGCCAGACCCTCAGCACCGACTTACAGGCCCCAAAATCTGAGACTTCTTCACCCTCAGCAGCCTCC TGCGCAGTATCAATATGAGCCTCCCAGTGCCCCCTCTTCCTCGTTCTCGAACTCTCAAGCCCCCAACTTT ATGCCCCCACGGCCTGACTTTGTCCCTTACCCTCCACCAGTTGCACCGTCAGCACAGGGCCCTCTCCCAC CCTGCCCAGTTAGGCCCCCTTACCCCAACCACCAGATGAGACACCCTTTCCCTGTGCCTCCCTGTTTTCC ACCCATGCCCCCTCCAATGCCTTGTCCCAACAACCCCCCAGTCTCCGGAGCACCTCCCGGCCAAGGCACT TTCCCCTTCATGGTCCCCCCTCCTTCCATGCCCCACCCCCCACCCCCTCCCGTCATGCCGCAGCAGGTTA ATTATCAGTACCCTCCTGGATACTCGCACAGTTTCCCGCCTCCCAATTTCAACAGTTTTCAGAACAACTC CAGTTCTTTCCCACCCAGTGCTAATAACAGCAGCACCCCTCATTTTAGACACCTCCCTCCATACTCACTT CCCAAGGCTCAGAGTGAGAGAAGGTCCCCAGAGAGGCTTAAGCACTACGATGACCACAGGCACCGAGATC ACAGTCACGGGCGAGGTGAGAGGCATCGGTCCCTGGAGCGCAGAGAGCGCGGCCGCAGCCCGGAGAGGAG AAGACCTGAGAGCCGCTACCGCTCGGAATATGATCGGGGGAGGACGCCACCGCCGCGCCATCGCAGCTAC GAAAGGAGCAGAGAGCGGGAACGAGAGAGACACAGGCACCGGGAAAACCGCAGATCACCGTCTCTGGAAA GGGCCTACAAAAAGGAATATAAGAGATCTGGAAGGAGCTATGGCTTACCAGTCACTCCTGAGCCGGCTGG ATGCACAGCAGAATTACCTGGGGAGATTATTAAAAATACAGATTCTTGGGCCCCACCCCAGGAGAATGTG AATCATCGTTCTCCAAGCAGGGAGAAGAAGAGAGCACGTTGGGAGGAGGAAAAAGACAGGTGGAGTGACA GCCAGAGCTCTGGCAAAGAGAAGAGCTATACGTCCATCAAAGAAAAGGAACCAGAGGAGGCGCCGCCTGA GAAAACCGAGGAAGAGGACGAAGAGCTCCTCAAGCCCGTGTGGATCCGATGCACACACTCGGAAAGTTAC TACTCCAGTGACCCCATGGATCAGGTGGGAGACTCTACTGTCGTAGGAACAAGCAGGCTCCGGGATCTGT ATGACAAATTTGAGGAAGAGTTGGGGAACAGGCAAGAGAAGGCCAAAGCTGCCCGGCCTCCATGGGAGCC CCCAAAGACAAAGCTGGATGAAGATTTAGAGAGCTCCAGTGAGTCTGAATGCGAGACGGACGAGGACAGC ACCTGCTCAAGCAGCTCGGACTCGGAGGTGTTCGATGTCATTGCAGAGATTAAACGCAAAAAGGCTCACC CTGACCGACTTCATGATGAACTTTGGTACAACGACCCAGGCCAGATGAACGACGGACCACTTTGCAAATG CAGTGCAAAAGCAAGACGCACAGGAATTCGGCACAGCATTTATCCCGGAGAAGAGGCAATCAAGCCCTGT CGTCCTATGACCAACAACGCTGGACGACTTTTCCACTACCGGATTACCGTCTCCCCGCCTACCAACTTTT TAACGGACAGGCCAACAGTCATAGAATACGATGATCATGAGTATATCTTTGAAGGATTTTCTATGTTTGC ACATGCCCCTCTGACCAATATTCCACTGTGTAAAGTAATTCGATTCAACATAGACTACACGATTCATTTT ATTGAAGAGATGATGCCTGAGAATTTTTGTGTGAAAGGACTTGAACTGTTTTCATTGTTCCTATTCAGAG ATATTTTGGAGTTATACGACTGGAATCTTAAAGGTCCTTTGTTTGAAGACAGCCCTCCCTGCTGTCCAAG ATTTCATTTCATGCCACGTTTTGTAAGATTTCTTCCAGATGGCGGCAAAGAAGTGTTATCAATGCACCAG ATTCTTCTCTACCTGCTTCGCTGTAGCAAGGCTTTGGTCCCTGAGGAGGAGATTGCCAATATGCTCCAAT GGGAGGAGCTCGAGTGGCAGAAATATGCGGAAGAGTGCAAAGGCATGATAGTTACCAACCCTGGGACGAA ACCAAGCTCTGTCCGCATCGATCAACTGGATCGTGAGCAGTTCAACCCTGAAGTGATCACTTTTCCGATT ATCGTTCACTTTGGGATACGCCCTGCACAGTTGAGTTATGCAGGAGACCCACAATACCAAAAATTATGGA AAAGTTACGTGAAACTTCGCCACCTCTTAGCAAATAGTCCCAAGGTCAAACAGACTGACAAACAGAAGCT GGCGCAGAGGGAGGAAGCGCTCCAGAAGATACGGCAGAAGAACACAATGCGTCGAGAAGTCACCGTGGAG CTGAGTAGCCAAGGATTCTGGAAAACTGGCATTCGTTCTGATGTCTGTCAGCACGCGATGATGCTGCCTG TTCTGACCCATCACATCCGATACCATCAATGCTTAATGCACTTGGACAAGTTGATAGGGTATACTTTTCA GGATCGTTGTCTGCTCCAGCTGGCCATGACTCATCCGAGTCATCATTTAAATTTTGGAATGAATCCTGAT CATGCCAGGAATTCTCTGTCTAACTGTGGAATTCGACAGCCCAAATATGGAGACAGAAAAGTTCATCACA TGCACATGCGGAAAAAAGGAATTAACACCCTAATAAATATCATGTCACGCCTCGGCCAAGATGATCCAAC TCCTTCAAGGATTAATCACAATGAAAGGTTGGAGTTCCTGGGAGATGCTGTTGTTGAGTTTCTGACCAGT GTCCACCTGTACTATTTGTTTCCTAGCTTGGAGGAAGGAGGCTTGGCAACCTATCGGACAGCCATTGTTC AGAATCAGCACCTCGCCATGCTCGCAAAGAAACTTGAACTGGATCGATTTATGCTGTATGCCCATGGGCC CGACCTATGTAGAGAATCAGACCTCCGGCATGCGATGGCCAATTGTTTCGAAGCATTGATAGGAGCTGTT TACTTGGAGGGGACCCTGGAGGAAGCCAAACAGTTGTTTGGACGCTTACTCTTTAATGATCCGGACCTGA GAGAAGTCTGGCTCAATTATCCTCTCCACCCACTCCAACTACAAGAGCCAAATACAGATCGACAACTTAT TGAAACTTCCCCAGTTCTACAGAAACTGACCGAGTTTGAAGAAGCAATTGGGGTGATCTTCACTCACGTC CGGCTTCTGGCACGGGCATTCACACTAAGAACTGTGGGCTTTAACCACCTGACCCTAGGCCACAATCAGA GGATGGAATTCCTGGGTGACTCCATAATGCAGCTGGTAGCCACGGAGTACTTGTTCATCCACTTCCCCGA CCACCACGAAGGACACTTGACGTTGTTACGAAGTTCTTTAGTGAACAACAGAACTCAAGCCAAGGTAGCA GAGGAACTGGGCATGCAAGAGTATGCCATCACCAACGACAAAACCAAGAGACCCGTGGCCCTAAGAACCA AGACTTTGGCAGACCTTTTGGAATCATTTATTGCAGCCCTGTACATCGACAAGGACTTGGAATACGTCCA TACTTTCATGAACGTCTGCTTCTTTCCACGTCTGAAGGAGTTCATTCTGAACCAGGACTGGAATGACCCC AAGTCGCAGCTGCAGCAGTGCTGCCTGACCCTGAGGACTGAAGGGAAAGAACCAGACATCCCCTTGTACA AGACTCTGCAGACAGTGGGGCCGTCCCACGCTAGAACCTACACGGTGGCCGTTTACTTCAAAGGAGAGAG GATAGGCTGTGGGAAAGGACCAAGTATTCAGCAGGCCGAAATGGGAGCAGCAATGGACGCACTTGAGAAA TATAACTTTCCCCAGATGGCTCATCAGAAGCGGTTCATTGAACGGAAATACAGACAAGAGTTAAAGGAAA TGAGGTGGGAAAGAGAGCATCAGGAGAGAGAGCCGGATGAGGCTGAAGACATAAAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217604 representing NM_001107655 Red=Cloning site Green=Tags(s) MQGNTCHRMSYHPGRGCPRGRGGHGARPSAPTYRPQNLRLLHPQQPPAQYQYEPPSAPSSSFSNSQAPNF MPPRPDFVPYPPPVAPSAQGPLPPCPVRPPYPNHQMRHPFPVPPCFPPMPPPMPCPNNPPVSGAPPGQGT FPFMVPPPSMPHPPPPPVMPQQVNYQYPPGYSHSFPPPNFNSFQNNSSSFPPSANNSSTPHFRHLPPYSL PKAQSERRSPERLKHYDDHRHRDHSHGRGERHRSLERRERGRSPERRRPESRYRSEYDRGRTPPPRHRSY ERSRERERERHRHRENRRSPSLERAYKKEYKRSGRSYGLPVTPEPAGCTAELPGEIIKNTDSWAPPQENV NHRSPSREKKRARWEEEKDRWSDSQSSGKEKSYTSIKEKEPEEAPPEKTEEEDEELLKPVWIRCTHSESY YSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGNRQEKAKAARPPWEPPKTKLDEDLESSSESECETDEDS TCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKPC RPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIHF IEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGPLFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMHQ ILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEECKGMIVTNPGTKPSSVRIDQLDREQFNPEVITFPI IVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQTDKQKLAQREEALQKIRQKNTMRREVTVE LSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNPD HARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLTS VHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAV YLEGTLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFEEAIGVIFTHV RLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVA EELGMQEYAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDP KSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEK YNFPQMAHQKRFIERKYRQELKEMRWEREHQEREPDEAEDIKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107655
ORF Size	4119 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107655.2, NP_001101125.2
RefSeq Size	4479 bp
RefSeq ORF	4122 bp
Locus ID	310159
MW	159 kDa

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