Med23 (NM_001277054) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Crsp3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217602 representing NM_001277054 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTACCAATGGAGACGCAACTGCAGAGCATTTTCGAGGAGGTGGTGAAAACAGAAATTATAGAAGAGG CTTTCCCAGGCATGTTTATGGATACCCCCGAGGATGAAAAAACAAAACTTATTAGCTGTTTGGCAGCCTT CCGGCAGTTTTGGAATGGACTTTCTCAGGAGTCTCATGAACAGTGTGTCCAGTGGATTGTTAAATTTATT CACGGCCAACATAGCCCGAAAAGGATTTCCTTTCTCTATGACTGCTTAGCCATGGCAGTTGAGACTGGTC TTCTTCCACCCAGGATGGTTTGTGAATCTTTGATAAACTCTGACACTCTTGAATGGGAAAGAACACAGCT TTGGGCCTTAACATTTAAGCTGGTTCGGAAAATCATTGGAGGAGTGGATTATAAGGGTGTTCGAGACCTC CTGAAAGTGATTTTGGAGAAAATCTTGACAATCCCGAATACTGTAAGCTCTGCTGTTGTCCAGCAGCTTC TGGCAGCAAGAGAGGTTATAGCGTATATCTTGGAACGAAATGCCTGCCTGTTGCCAGCCTACTTTGCAGT CACAGAGATCAGAAAATTATATCCCGAGGGAAAACTTCCACACTGGTTACTAGGAAACTTGGTATCAGAC TTTGTGGATACCTTCAGGCCCACAGCGAGGATAAACTCCATTTGTGGCCGTTGCAGTCTTCTGCCAGTTG TGAACAACTCGGGAGCCATCTGTAACTCATGGAAACTGGACCCTGCAACTCTCCGCTTTCCCTTGAAAGG CCTTTTGCCATACGATAAGGATCTGTTTGAACCCCAGACTGCTTTGTTGAGATATGTACTGGAGCAGCCT TACTCCAGGGATATGGTCTGCAATATGCTAGGTTTAAACAAGCAGCACAAGCAGCGCTGCCCTGTGCTGG AGGACCAGTTGGTGGACCTGGTGGTGTACGCCATGGAGAGGTCTGAGACCGAGGAGAAGTTTGACGATGG GGGAACCAGCCAGCTCCTGTGGCAGCACCTTTCCAGCCAGCTCATTTTCTTTGTGCTTTTCCAGTTTGCC AGTTTCCCACATATGGTTCTGTCTCTCCACCAGAAGTTAGCAGGGCGAGGACTGATTAAAGGGCGAGACC ATCTGATGTGGGTTCTGCTGCAGTTCATCTCGGGAAGTATTCAGAAAAATGCGCTTGCTGACTTCCTCCC TGTCATGAAGCTCTTTGACCTGCTGTACCCAGAGAAGGAATGTATCCCAGTTCCTGATATTAACAAACCA CAGTCAACACATGCCTTTGCAATGACTTGTATTTGGATTCATCTTAATAGAAAAGCTCAAAATGACAACT CCAAGCTGCAGATCCCCATACCCCACTCCCTGAAGCTGCACCACGAGTTCCTACAGCAGAGTCTGAGAAA CAAAAGTTTGCAGATGAATGACTATAAGATCGCCCTGCTGTGTAATGCGTATTCTACAAATTCAGAGTGT TTCACCTTACCTATGGGCGCTCTGGTAGAGACCATTTATGGCAATGGAATTATGAGAGTGCCTCTCCCCG GGACAAGCTGTCTGGCCTCAGCATCAGTAACTCCCTTACCAATGAACCTCCTGGATTCTCTGACAGTGCA TGCCAAGATGAGCCTTATTCACAGCATTGCAACCAGAGTGATAAAACTGGCTCATACAAAATCCAGTGTC GCATTGGCTCCAGCCCTGGTGGAAACTTACAGTCGTTTACTGGTCTACATGGAAATAGAATCTTTGGGCA TCAAAGGATTTATCAGTCAGCTCCTGCCCACTGTCTTCAAGTCCCATGCCTGGGGCATCCTGCACACGCT GCTGGAGATGTTCAGCCACCGGATGCACCACATTCAGCCACATTACCGAGTTCAGCTCCTGAGCCATCTC CACACCCTGGCTGCAGTTGCACAAACCAACCAGAACCAGCTCCATCTGTGCGTAGAAAGTACTGCGCTGA GGCTCATCACGGCGCTGGGGAGCTCCGAGGTCCAGCCGCAGTTTACACGCTTCCTCAACGATCCCAAAAC AGTGCTGTCCGCAGAATCTGAAGAGCTGAACCGAGCCTTGATCCTGACCTTGGCCAGAGCAACCCACGTC ACAGATTTTTTTACAGGATCTGATTCAATACAGGGAACTTGGTGTAAAGATATCCTTCAGACCATCATGA CTTTTACTCCTCATAACTGGGCTTCGCACACCCTTAGCTGTTTTCCAGCCCCTCTGCAGGCATTCTTCAA GCAAAATAATGTACCTCAGGAAAGCCGTTTTAATCTGAAAAAAAACGTGGAAGAAGAGTACAGGAAGTGG AAGTCAATGACTGATGAAAACGAGATCATCACCCAGTTCTCCATGCAAGGCTTCCCTCCGCTCTTCCTCT GTCTCCTCTGGAAAATGCTCCTGGAAACGGATCACATTAGTCAGATTGGGTACAAAGTACTAGAGAGAAT TGGGGCCAGGGCCTTGGTGGCCCACGTGAGAACCTTTGCAGATTTCTTGGTGTATGAGTTCTCTACATCA GCAGGAGGTCAGCAACTCAATAAATGCATTGAGATTCTCAATGACATGGTGTGGAAGTACAACATTGTCA CACTGGACAGACTAATTCTCTGCCTGGCTATGCGTAGTCATGAAGGAAATGAAGCTCAGGTTTGTTATTT CATAATTCAGTTGCTATTGCTAAAACCAAATGACTTCCGAAACCGAGTGAGTGACTTTGTGAAGGAGAAC TCCCCAGAGCACTGGCTGCAGAGCGACTGGCACACCAAACACATGAGCTACCACAAGAAATATCCAGAGA AGTTGTACTTTGAAGGCCTGGCAGAACAGGTTGACCCTCCTGTACCAATTCAGTCCCCGTATCTGCCCAT CTACTTTGGAAACGTGTGTCTCCGGTTTCTTCCAGTATTTGATATAGTGATCCACAGGTTTTTAGAGTTG CTGCCAGTGTCCAAATCACTAGAGACTCTCCTGGATCACCTAGGGGGCTTGTACAAATTCCACGACCGGC CGGTGACTTACCTGTATAACACTTTACACTATTATGAAATGTGCCTGAGAAACCGTGACCATCTCAAACG AAAACTCGTCCATGCCATCATTGGCTCCCTCAAAGATAACCGGCCACAGGGCTGGTGTCTCAGTGACACC TACCTGAAGCATGCTATGAATGCACGGGAGGACAACCCCTGGGTTCCAGAGGACTCCTACTACTGCAAGC TGATTGGCCGGCTGGTAGACACCATGGCTGGCAAGTCTCCGGGACCCTTCCCAAACTGTGACTGGAGATT CAATGAGTTCCCCAACCCTGCTGCCCATGCTCTGCATGTGACGTGTGTGGAACTCATGGCCTTGGCAGTT CCAGGCAAAGATGTCGGGAATGCCCTTCTCAATGTTGTCCTGAAAAGCCAGCCCTTAGTGCCAAGGGAGA ACATCACAGCATGGATGAATGCAATTGGTCTGATCATCACGGCCCTCCCAGAACCATACTGGATTGTGCT TCATGACCGAATCGTGAGTGTAATCAGCAGTTCTAGCCTGACGTCAGAGACAGAGTGGGTTGGTTATCCG TTCCGCCTCTTCGACTTCACCGCTTGTCACCAGTCCTACTCTGAGATGAGCTGCAGCTACACGTTAGCGC TGGCTCACGCCGTGTGGCACCACTCCAGCATCGGACAGCTCTCGCTTATCCCGAAGTTTCTCACTGAAGT GCTTCTTCCCGTGGTGAAGACTGAATTCCAGTTGCTTTACGTGTACCATCTCGTAGGGCCATTTTTACAG CGATTCCAGCAGGAGAGAACCCGGTGCATGATCGAGATTGGAGTGGCATTTTATGACATGCTGCTGAATG TAGACCAGTGCAGCACCCACCTCAATTACATGGACCCCATCTGCGACTTCCTATACCATATGAAGTATAT GTTCACTGGAGACAGTGTGAAGGAGCAGGTAGAGAAGATCATCTGTAATTTAAAGCCAGCTTTGAAGCTT CGTCTTCGGTTCATCACGCACATCAGCAAGATGGAGCCGGCAGTGCCACCCCAGGCCCTCAGCAGCGGGT CTCCAGCCCCTCAGGCCAACCAGGTGCCCACAGCTCTGCCCGTGACTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217602 representing NM_001277054 Red=Cloning site Green=Tags(s) MVPMETQLQSIFEEVVKTEIIEEAFPGMFMDTPEDEKTKLISCLAAFRQFWNGLSQESHEQCVQWIVKFI HGQHSPKRISFLYDCLAMAVETGLLPPRMVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDL LKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSD FVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQP YSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFA SFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKECIPVPDINKP QSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLKLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSEC FTLPMGALVETIYGNGIMRVPLPGTSCLASASVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHTKSSV ALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSHRMHHIQPHYRVQLLSHL HTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTLARATHV TDFFTGSDSIQGTWCKDILQTIMTFTPHNWASHTLSCFPAPLQAFFKQNNVPQESRFNLKKNVEEEYRKW KSMTDENEIITQFSMQGFPPLFLCLLWKMLLETDHISQIGYKVLERIGARALVAHVRTFADFLVYEFSTS AGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKEN SPEHWLQSDWHTKHMSYHKKYPEKLYFEGLAEQVDPPVPIQSPYLPIYFGNVCLRFLPVFDIVIHRFLEL LPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMCLRNRDHLKRKLVHAIIGSLKDNRPQGWCLSDT YLKHAMNAREDNPWVPEDSYYCKLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAV PGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSSSLTSETEWVGYP FRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPVVKTEFQLLYVYHLVGPFLQ RFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKL RLRFITHISKMEPAVPPQALSSGSPAPQANQVPTALPVTQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001277054
ORF Size	4110 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001277054.1, NP_001263983.1
RefSeq Size	4727 bp
RefSeq ORF	4113 bp
Locus ID	309565
MW	156.6 kDa

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