Acin1 (NM_001170468) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217593 representing NM_001170468 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGAGACGGAAACGACCGAACAGTTCCGGAGGAACCCGGGGAATTCTGAGCGGTAATAGAGGGGTAG ACTATGGCAGTGGGCGGGGCCAGTTCGGTACGTTCGAAGGGCGTTGGCGGAAATTACCGAAGATGCCCGA AGCCGTCGGGACGGACCCGAGTACCTCACGCAAGATGGCGGAGCTGGAGGAGGTGACTCTGGACGGGAAG CCTCTTCAGGCTCTGCGGGTGACCGACCTGAAGGCCGCACTGGAGCAGCGAGGCCTAGCCAAGAGCGGGC AGAAGAGTGCCCTGGTCAAGCGGCTCAAAGGGGCTCTAATGCTAGAAAATTTACAAAAACACTCAACACC CCATGCTGCATTCCAGCCAAATTCTCAGATTGGGGAGGAGATGAGCCAGAACAGTTTCATAAAGCAGTAC CTGGAAAAGCAGCAGGAGCTACTTAGGCAGCGCCTAGAACGGGAAGCTCGAGAGGCTGCAGAACTTGAAG AAGCTTCAGCTGAGTCGGAGGACGAGATGACCCATCCTGAGGGAGTGGCTTCCCTGCTGCCTCCTGACTT TCAGAGCAGCCTGGACAGACCAGAGCTGGAGCTCAGCACACATTCACCTCGAAAGAGCTCCTCTTTTTCT GAAGAGAAAGGTGAATCCGATGATGAGAAACCAAGGAAAGGAGAAAGACGATCATCCCGGGTCAGACAGG CAAGAGCTAAACTCCCTGAGTACAGTCAGGCTGCTGAGGAGGAAGAGGACCAAGAGACGCCATCTAGAAA CCTGAGGGTCAGAGCAGATCGAAATTTAAAAGTAGAGGAGGAGGAGGAAGAGGAGGAAGAAGAAGATGAT GAAGAAGAGGAAGAGGAGGTAGATGAGGGACAGAAATCTAGAGAAGCCGAGGCACCAATCCTGAAACAGT TTGAGGATGAAGACGGGGAGGAGATGACCAGAGCAAAACCAGAAAAGGTAGTGGATGAGAAACCCCCAAT CATAAGATCCCAGGAAAAGGATGAGTTAGAGAAAGGAGGGCGAGTTACAAGATCCCAGGAAGAAGCTAGA AGAAGTCATCTGGCCAGACAACAACAAGAGAAAGAGACACAAATTGTTTCTCTCCCTCAAGAAGAAAATG AGGTAAAATCTTCACAAGGTTTGGAAGAAAAATCCCAGTCTCCTTCCCTGCCTCCCCTCCCTGAAGACCT AGAGAAGGCCCCTGTCGTGCTGCAGCCAGAGCAAATAGTCAGTGAAGAGGAAACACCCCCACCTTTACTT ACCAAGGAAGCTTCATCTCCACCAACTCATATGCAGCTCCAGGATGAAGAAGAAGTGGAGCCAGCGGAAG GCCCAGCACCCCCTGTCCTCATTCAGTTATCTCCTCCTGGTACAGATGCTGGAACCAGGGAGCCCATAGT GTCTCCCCACACAGTGCAGCTGCTCAGAGGCCTGTCTCCTCTGTCAAGTACTGCAGACACCAAAGCAGAG TCTCCAGCAGAGAGAGTGTCAGAAGAGAGTGTCCTGCCCCTGGCACAGAGAAGCATACTGCCCGAACACT CAGCCCAGAAAGGTGCTGAAAGTGAGCGTGAAAAGTCTGCTCCCCTTCTCCCTCTAACAAGAGAGGAGTT AGCACCCGCCAAAGGGATCACTGAGGAGTCTGTGAAGAAACAGTCTTTGGAACAGGAGGAGGGCAGAAGA GCTTCCCACACTCTCTTCCCAGACCACAGTTTGAAACCGTCAGCCGACTCATCCTCGAGCCGATCATCCT CACCTTCATCCTCCAGCTCTAGATCCAGATCTCGATCTCCTGACAGTTCAGCTTCCCGCTCACAGTCCTC TCCAAGATCTAAACAGAGAGAGGTGTCTCAAGCGCGAGTTCACGCCAATCCTCATGATAGAGCCAAGATG GGCTCCAGGTCAACATCAGAGTCTAGGTCACGTTCTCGATCTCGTTCCCGTTCCGCATCAAGCGGCAGCA GAAAATCTCTGAGCCCTGGAGTCTCCAGAGACGGCAACACCACCTACACTGAAACCAAAGATCCCTCTTG TGGTCAGGAGGCTGCAGCTCCATCAGTGCCACAGCTGCAGGTCCTTGAACCAAAGGAGAAGGCTCCCACG TGCTCAGCCTCTGTCCGGGGGAGGCATATGAGCCAGCCTGAGCCAGAGCAAAAGCACGTGATCCAGAGGT TACAGCCGGAGCAGGAGAGCCCAAAGAAGTGTGAAGCTGAAGAGGCAGAGCCACCAGCTGCCACCCAGCC TCAGACCTCAGAGCCTCAGCTCTCTCAGCTGCCAGAGGCGGAAAGGACTCCTCACACCGCTGAAGAGAAG GAGGACGTGACCATGGACACAAGTGAAAACAGACCTGAAAATGAGGTTCCTGAGCCTCCTGTGCCTATTG CAGACCAAGTCAGCAATGATGACCGCCCAGAAGGCAGTGCTGAAGAAGAGGAGAGGAAAGAGAGCTCGAT GCCCAAATCATTCAAGAGGAAAATCTCCGTTGTCTCAGCTACCAAGGGGGTGCAAGCTGGAAACAGTGAC ACAGAGGGGGGCCAGCCTGGTCGAAAGCGTCGTTGGGGAGCCAGTACTGCTGCCACACAGAAGAAACCTT CTATCAGTATCACCACTGAGTCACTCAAGAGCCTCATCCCCGACATCAAACCCCTGGCGGGGCAGGAGGC TGTTGTGGATCTTCATGCTGATGACTCCCGAATCTCTGAGGATGAGACAGAGCGTAATGGCGACGATGGG ACCCATGACAAAGGACTGAAAATATGCCGGACAGTCACTCAGGTAGTACCTGCAGAGGGCCAGGAGAACG GGCAGAGGGAAGAGGAAGAAGAGAAAGAGCCTGAAGCAGAGCCTCCTGCGCCTCCCCAGGCGTCGGTGGA GGCTGCCTTGCCCCCACCTGTGGAGCATGAAGTAAAGAAAGTAACATTAGGAGATACCTTAACCCGGAGG TCCATTAGCCAACAGAAGTCTGGAGTTTCCATTACAATTGATGACCCAGTCCGAACTGCCCAGGTGCCCT CCCCACCCAGGGGCAAGATCAGTAACATTGTCCACATCTCCAACTTGGTTCGTCCCTTCACTCTAGGCCA GCTAAAGGAGTTATTAGGGCGTACAGGGACTTTGGTGGAGGAGGCCTTCTGGATTGACAAGATCAAATCT CATTGCTTTGTGACGTACTCAACAGTAGAGGAAGCCGTGGCCACCCGGACAGCTCTGCACGGGGTCAAGT GGCCCCAATCCAACCCCAAATTCCTCTGTGCTGACTATGCTGAGCAAGATGAGCTGGACTATCACCGGGG ACTCTTGGTAGATCGGCCATCTGAAGCTAAGACAGAGGAACAGGGAGCACCAAGGCCCTTGCATCCCCCA CCCCCACCCCCAGTCCAGCCACCACCTCATCCCAGGGCTGAGCCACGGGAGCAGGAAAGGGCTGTACGGG AGCAGTGGGCAGAACGGGAACGGGAAATGGAGCGGCGGGAGAGGACTCGGTCTGAGCGAGAATGGGATCG GGACAAAGTTCGAGAGGGGCCCCGCTCCCGATCACGGTCCCGTGACCGCCGCCGCAAAGAGCGAGCAAAA TCTAAAGAAAAGAAGAGTGAGAAGAAAGAGAAAGCTCAGGAGGAGCCGCCTGCCAAGTTGCTGGATGACC TCTTCCGTAAGACCAAGGCAGCTCCCTGCATCTATTGGCTCCCACTGACTGAGAGCCAGATTGTTCAGAA GGAGGCAGAGCAAGCAGAACGGGCCAAGGAGCGGGAGAAGCGGCGAAAGGAACGAGAAGAAGAAGAACAG AAGGAGCGGGAGAAGGAAGCTGAGCGGGAACGGAACCGACAGCTAGAACGGGAGAAACGGAGGGAGCACA GCAGGGAGAGAGAGAGGGACAGGGAAAGAGAGAGGGACAGGGGCGACCGAGATCGGGACAGGGAAAGAGA CCGAGACCGAGGCAGGGAGAGGGATCGCAGAGACACCAAGCGACACAGCAGAAGCCGGAGTCGAAGCACA CCTGTACGGGACCGGGGTGGGCGCCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217593 representing NM_001170468 Red=Cloning site Green=Tags(s) MWRRKRPNSSGGTRGILSGNRGVDYGSGRGQFGTFEGRWRKLPKMPEAVGTDPSTSRKMAELEEVTLDGK PLQALRVTDLKAALEQRGLAKSGQKSALVKRLKGALMLENLQKHSTPHAAFQPNSQIGEEMSQNSFIKQY LEKQQELLRQRLEREAREAAELEEASAESEDEMTHPEGVASLLPPDFQSSLDRPELELSTHSPRKSSSFS EEKGESDDEKPRKGERRSSRVRQARAKLPEYSQAAEEEEDQETPSRNLRVRADRNLKVEEEEEEEEEEDD EEEEEEVDEGQKSREAEAPILKQFEDEDGEEMTRAKPEKVVDEKPPIIRSQEKDELEKGGRVTRSQEEAR RSHLARQQQEKETQIVSLPQEENEVKSSQGLEEKSQSPSLPPLPEDLEKAPVVLQPEQIVSEEETPPPLL TKEASSPPTHMQLQDEEEVEPAEGPAPPVLIQLSPPGTDAGTREPIVSPHTVQLLRGLSPLSSTADTKAE SPAERVSEESVLPLAQRSILPEHSAQKGAESEREKSAPLLPLTREELAPAKGITEESVKKQSLEQEEGRR ASHTLFPDHSLKPSADSSSSRSSSPSSSSSRSRSRSPDSSASRSQSSPRSKQREVSQARVHANPHDRAKM GSRSTSESRSRSRSRSRSASSGSRKSLSPGVSRDGNTTYTETKDPSCGQEAAAPSVPQLQVLEPKEKAPT CSASVRGRHMSQPEPEQKHVIQRLQPEQESPKKCEAEEAEPPAATQPQTSEPQLSQLPEAERTPHTAEEK EDVTMDTSENRPENEVPEPPVPIADQVSNDDRPEGSAEEEERKESSMPKSFKRKISVVSATKGVQAGNSD TEGGQPGRKRRWGASTAATQKKPSISITTESLKSLIPDIKPLAGQEAVVDLHADDSRISEDETERNGDDG THDKGLKICRTVTQVVPAEGQENGQREEEEEKEPEAEPPAPPQASVEAALPPPVEHEVKKVTLGDTLTRR SISQQKSGVSITIDDPVRTAQVPSPPRGKISNIVHISNLVRPFTLGQLKELLGRTGTLVEEAFWIDKIKS HCFVTYSTVEEAVATRTALHGVKWPQSNPKFLCADYAEQDELDYHRGLLVDRPSEAKTEEQGAPRPLHPP PPPPVQPPPHPRAEPREQERAVREQWAEREREMERRERTRSEREWDRDKVREGPRSRSRSRDRRRKERAK SKEKKSEKKEKAQEEPPAKLLDDLFRKTKAAPCIYWLPLTESQIVQKEAEQAERAKEREKRRKEREEEEQ KEREKEAERERNRQLEREKRREHSRERERDRERERDRGDRDRDRERDRDRGRERDRRDTKRHSRSRSRST PVRDRGGRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001170468
ORF Size	4017 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001170468.1, NP_001163939.1
RefSeq Size	4636 bp
RefSeq ORF	4020 bp
Locus ID	305884
MW	151 kDa

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