Ttbk1 (NM_001271230) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1559518
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217583 representing NM_001271230 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTGCCTAGCGGCTGCTCTTAAAGACGAAACCAACATGAGTGGGGGAGGGGAGCAGGCCGACATCC TGCCGGCCAACTACGTGGTCAAAGATCGCTGGAAGGTGCTGAAAAAGATCGGGGGCGGGGGCTTCGGTGA GATCTATGAGGCCATGGACCTGCTGACCAGGGAGAACGTGGCCCTGAAGGTGGAGTCAGCCCAACAGCCT AAGCAGGTCCTCAAGATGGAGGTGGCTGTGCTCAAAAAGCTTCAAGGGAAGGACCACGTGTGCAGGTTCA TTGGCTGTGGCAGGAATGAGAAGTTTAACTATGTGGTGATGCAGCTCCAGGGTCGGAACCTGGCTGACTT GCGCCGCAGCCAGCCAAGGGGCACTTTCACACTGAGTACCACACTGCGTCTGGGCAAGCAGATTCTGGAG TCCATTGAAGCCATTCACTCCGTGGGCTTCCTGCACCGTGACATCAAGCCGTCCAACTTTGCCATGGGCC GGCTGCCCTCCACCTACAGAAAGTGCTACATGTTGGACTTTGGGCTGGCCCGGCAGTACACTAACACCAC TGGGGACGTCCGGCCTCCTCGGAATGTGGCCGGGTTCCGGGGGACTGTCCGCTACGCGTCGGTCAACGCT CACAAGAATCGGGAGATGGGCCGCCATGATGACCTGTGGTCCCTCTTCTACATGCTGGTGGAGTTTGCTG TGGGGCAGCTGCCCTGGAGGAAGATCAAGGACAAGGAGCAGGTAGGGATGATCAAGGAGAAGTATGAGCA CCGGATGCTACTGAAGCACATGCCTTCCGAGTTCCATCTCTTCCTGGACCACATCGCCAGCCTCGACTAC TTCACCAAGCCCGACTACCAGTTGATCATGTCGGTGTTTGAGAACAGCATGAAGGAACGGGGCATTGCTG AGAATGAGGCCTTTGACTGGGAGAAGGCAGGCACTGATGCCCTTCTGTCCACGAGCACCTCCACCCCACC CCAGCAGAACACACGGCAGACGGCAGCCATGTTTGGAGTGGTCAATGTGACACCTGTGCCCGGGGACCTG CTTCGTGAGAACACTGAGGATGTGCTCCAGGGAGAACACCTGAGTGACCAGGAGAATGCACCCCCAATCC TGCCTGGGAGGCCTCCGGAGGGCTTGGGTCCTGGTCCCCACCTTGTCCCCCACCCTGGGGGTCCTGAGGC TGAAGTGTGGGAGGAGACAGATGTCAACCGTAACAAACTGCGCATCAACATTGGCAAAACACCCTGTGTG GAAGAAGAGCAGAGTCGAGGAGTCGGGGTCCCCAGCTCCCCAGTGCGTGCTCCCCCAGACTCCCCTACAA CCCCAGTGCGGTCTTTACGCTATCGGAGGGTCAATAGCCCAGAGTCGGAAAGGCTGTCTACAGCAGCTGA TGGGCGGGCAGAACTTCAGGAGAGGAGGTCACGGATGGATCTGCCTGGCTCACCCTCACGCCAGGCCTGC TCCTCACAACCAGCTCAGATGCTGTCAGTGGACACAGGCCATGCCGACAGGCAGGCCAGTGGCCGCATGG ACGTGTCAGCCTCTGTGGAGCAGGAGGCCCTGAGCAACGCCTTCCGCTCAGTGCCACTGGCTGAGGAGGA AGACTTTGACAGCAAGGAGTGGGTCATTATTGACAAGGAGACAGAGCTCAAGGACTTCCCTCCTGGGGCT GAGCCCAGCACATCAGGTACCACAGATGAAGAACCTGAGGAGCTGCGGCCTTTGCCTGAGGAGGGCGAGG AGAGGAGGCGTCTGGGGACAGAGCCTACTGTCAGGCCTCGTGGACGTGGTATGCAGACGCTGGCAGAGGA GGACCCACGGCAGATGCTGCCCCAGCCTGCACCACCTCAGTTGAGCCAGGCTGATGGCCGGTCAGAGACA TCGCAGCCTCCCACGCCTGGCAGCCCTTCCCACTCACCCCTGCACTCGGGACCCCGCCCTCGACGGAGAG AGTCGGATCCTACAGGCCCTCAGAGACAGGTGTTCTCTGTGGCGCCCCCGTTTGAGGTGAATGGCCTTCC ACGAGCTGTGCCTCTGGGCTTGCCCTACCAGGACTTCAAGAGAGACCTCTCTGATTATCGAGAACGGGCC CGGCTGCTCAACAGGGTCCGAAGGGTGGGCTTCTCGCACATGCTGCTCACCACACCGCAGGTCCCATTGG CTCCTTTCCAGCCTCAAGCCAATGGGAAAGAGGGTGAAGAGGAAGAGGAGGAGGATGAAGAGGAAGAGGA GGAAGAAGAGGAGGAAGATGAGGAAGACGAGGAGGAAGAGGATGAGGAAGATGAAGATGAGGAGGAGGAG GAGGCAGGGGCTCTTGGGGAGGTGCTGGGGCCTCGAAGCGGCTCCAGCAGTGAGGGGAGTGAACGGAGCC AGGAGGGCGCCCCATCCACACTGCTGGCTGACGACCAGAAAGAGGCCAGGGGCCGGGCCTCCATGGCTGA TGGGGACCTGGAGCCTGAGGAGGGCTCCAAAACGCTGGTGCTCGTCTCCCCTGGTGACATGAAGAAGTCA CCAGTCACTGCCGAACTGGCCCCTGACCCTGATCTGGGCACCTTGGCTGCACTCACCCCTCAGCACGAGC GGCCTCAGCCTACTGGAAGCCAGCTGGATGTGTCAGAGCCGGGCACCCTGTCCTCCATCCTCAAGTCTGA ACCCAAGCCCTCAGGACCTGGAGCAGGGGTTGGGGTTGGGCTGGTGGCCCCTGGGGCTGGGGTCACCGCA GTGACATCACCCTTCACCAAAGTCGAAAGGACCTTTGTTCACATTGCAGAGAAATCCCACCTCAATGTCA TGTCTTCCGGAGGACAGGCCCCCAGGCCAGAGGAGCTGAGCACTGGGGGCGAGTTGGGCCTAGAGATGCT CTCTGAGGTGGGCGCTGCAGAGGAGGGCGCCCCACAGCCTCTGGAGAACGGCATGGCTTTGGTAGGCCCG GATGGGACGGAGATGGAAAGTTGTGCCCTGTCTGGCCCTCCTGGGGAGACACCCTCAGAAGTAGTCACAG ACTCATTGCCCGATGGCCCAGCCCTTGCCGATGAACCGGCTCCAGCATCCCCACAAGAGCCAATAACCAA GAAAGGGACCATCATCTCCCCCAGCCGCCATGCCATGCCAGGATCTCGCCCCAGGAGCCGCATTCCTGTC TTGTTGTCTGAAGAGGACACAGGCTCAGAGCCTTCGGGCTCACTGTCGGCCAAAGAGCGGTGGAGCAAGA GGGCTCGGCCACAGCAGGATCTGGCTCGCCTCGTGATGGAGAAGAGGCAGGGTCGGCTACTGCTGCGGCT GGCCTCAGGGGCCTCCTCCTCCTCCAGCGAGGAGCAGCGCCGTGCCTCTGAGACACTCTCAGGCACCGGC TCCGAGGAGGACACGCCTGCCTCAGAGCCTGCAGCAGCCTTGCCTAGGAAGGCTGGGCGGGCAGCGACCA CCCGGAGCCGGATCCCCCGCCCCATCAGTGTGTCCATGCCTGCAGAAGGCCAGCAGCTTCCTGGTAGACC CCACGGTGCGGCCTCAGCGACCGACTCGGCCATCACCAGCAGGCTCCAGCTGCAGAAGCCCCCAGGGTTG GCCACTGCTGCTGACCACCGTCCCAAACAGCCCGCAAGCCGGGCTTCCGGCCCAGGTCGCGCCCAAGTCT CCAAGCCCGCAGCACCCCGCAGTCCCGGCCTCCCCACCTCCACCGCGCGCCACTCCAGCGGGTCCCCGCG GAGCCAGTCCCTGTCCCGCAAAGAGAGCTCCTCCCCCTCGCATCAAGCCCGGCCTGGTTTCCCTCCATCT CGGGGGGTCCTCCAAGTCAGATCTCAGTCGGAGGCCTCCCCGGGGGGCCCCAAGAAAGGACCCAAGGGGA AACAACTGCAGACTCTGCGCGCAGCAACCAAAGGCCGGCCGGCGGTTTCAGAGGGTCGGCCCGGGAACAG A AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217583 representing NM_001271230 Red=Cloning site Green=Tags(s) MQCLAAALKDETNMSGGGEQADILPANYVVKDRWKVLKKIGGGGFGEIYEAMDLLTRENVALKVESAQQP KQVLKMEVAVLKKLQGKDHVCRFIGCGRNEKFNYVVMQLQGRNLADLRRSQPRGTFTLSTTLRLGKQILE SIEAIHSVGFLHRDIKPSNFAMGRLPSTYRKCYMLDFGLARQYTNTTGDVRPPRNVAGFRGTVRYASVNA HKNREMGRHDDLWSLFYMLVEFAVGQLPWRKIKDKEQVGMIKEKYEHRMLLKHMPSEFHLFLDHIASLDY FTKPDYQLIMSVFENSMKERGIAENEAFDWEKAGTDALLSTSTSTPPQQNTRQTAAMFGVVNVTPVPGDL LRENTEDVLQGEHLSDQENAPPILPGRPPEGLGPGPHLVPHPGGPEAEVWEETDVNRNKLRINIGKTPCV EEEQSRGVGVPSSPVRAPPDSPTTPVRSLRYRRVNSPESERLSTAADGRAELQERRSRMDLPGSPSRQAC SSQPAQMLSVDTGHADRQASGRMDVSASVEQEALSNAFRSVPLAEEEDFDSKEWVIIDKETELKDFPPGA EPSTSGTTDEEPEELRPLPEEGEERRRLGTEPTVRPRGRGMQTLAEEDPRQMLPQPAPPQLSQADGRSET SQPPTPGSPSHSPLHSGPRPRRRESDPTGPQRQVFSVAPPFEVNGLPRAVPLGLPYQDFKRDLSDYRERA RLLNRVRRVGFSHMLLTTPQVPLAPFQPQANGKEGEEEEEEDEEEEEEEEEEDEEDEEEEDEEDEDEEEE EAGALGEVLGPRSGSSSEGSERSQEGAPSTLLADDQKEARGRASMADGDLEPEEGSKTLVLVSPGDMKKS PVTAELAPDPDLGTLAALTPQHERPQPTGSQLDVSEPGTLSSILKSEPKPSGPGAGVGVGLVAPGAGVTA VTSPFTKVERTFVHIAEKSHLNVMSSGGQAPRPEELSTGGELGLEMLSEVGAAEEGAPQPLENGMALVGP DGTEMESCALSGPPGETPSEVVTDSLPDGPALADEPAPASPQEPITKKGTIISPSRHAMPGSRPRSRIPV LLSEEDTGSEPSGSLSAKERWSKRARPQQDLARLVMEKRQGRLLLRLASGASSSSSEEQRRASETLSGTG SEEDTPASEPAAALPRKAGRAATTRSRIPRPISVSMPAEGQQLPGRPHGAASATDSAITSRLQLQKPPGL ATAADHRPKQPASRASGPGRAQVSKPAAPRSPGLPTSTARHSSGSPRSQSLSRKESSSPSHQARPGFPPS RGVLQVRSQSEASPGGPKKGPKGKQLQTLRAATKGRPAVSEGRPGNR SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271230
ORF Size	3921 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271230.1, NP_001258159.1
RefSeq Size	3924 bp
RefSeq ORF	3924 bp
Locus ID	316229
MW	141.6 kDa

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