Ttbk1 (NM_001271230) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1559518
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217583 representing NM_001271230 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTGCCTAGCGGCTGCTCTTAAAGACGAAACCAACATGAGTGGGGGAGGGGAGCAGGCCGACATCC TGCCGGCCAACTACGTGGTCAAAGATCGCTGGAAGGTGCTGAAAAAGATCGGGGGCGGGGGCTTCGGTGA GATCTATGAGGCCATGGACCTGCTGACCAGGGAGAACGTGGCCCTGAAGGTGGAGTCAGCCCAACAGCCT AAGCAGGTCCTCAAGATGGAGGTGGCTGTGCTCAAAAAGCTTCAAGGGAAGGACCACGTGTGCAGGTTCA TTGGCTGTGGCAGGAATGAGAAGTTTAACTATGTGGTGATGCAGCTCCAGGGTCGGAACCTGGCTGACTT GCGCCGCAGCCAGCCAAGGGGCACTTTCACACTGAGTACCACACTGCGTCTGGGCAAGCAGATTCTGGAG TCCATTGAAGCCATTCACTCCGTGGGCTTCCTGCACCGTGACATCAAGCCGTCCAACTTTGCCATGGGCC GGCTGCCCTCCACCTACAGAAAGTGCTACATGTTGGACTTTGGGCTGGCCCGGCAGTACACTAACACCAC TGGGGACGTCCGGCCTCCTCGGAATGTGGCCGGGTTCCGGGGGACTGTCCGCTACGCGTCGGTCAACGCT CACAAGAATCGGGAGATGGGCCGCCATGATGACCTGTGGTCCCTCTTCTACATGCTGGTGGAGTTTGCTG TGGGGCAGCTGCCCTGGAGGAAGATCAAGGACAAGGAGCAGGTAGGGATGATCAAGGAGAAGTATGAGCA CCGGATGCTACTGAAGCACATGCCTTCCGAGTTCCATCTCTTCCTGGACCACATCGCCAGCCTCGACTAC TTCACCAAGCCCGACTACCAGTTGATCATGTCGGTGTTTGAGAACAGCATGAAGGAACGGGGCATTGCTG AGAATGAGGCCTTTGACTGGGAGAAGGCAGGCACTGATGCCCTTCTGTCCACGAGCACCTCCACCCCACC CCAGCAGAACACACGGCAGACGGCAGCCATGTTTGGAGTGGTCAATGTGACACCTGTGCCCGGGGACCTG CTTCGTGAGAACACTGAGGATGTGCTCCAGGGAGAACACCTGAGTGACCAGGAGAATGCACCCCCAATCC TGCCTGGGAGGCCTCCGGAGGGCTTGGGTCCTGGTCCCCACCTTGTCCCCCACCCTGGGGGTCCTGAGGC TGAAGTGTGGGAGGAGACAGATGTCAACCGTAACAAACTGCGCATCAACATTGGCAAAACACCCTGTGTG GAAGAAGAGCAGAGTCGAGGAGTCGGGGTCCCCAGCTCCCCAGTGCGTGCTCCCCCAGACTCCCCTACAA CCCCAGTGCGGTCTTTACGCTATCGGAGGGTCAATAGCCCAGAGTCGGAAAGGCTGTCTACAGCAGCTGA TGGGCGGGCAGAACTTCAGGAGAGGAGGTCACGGATGGATCTGCCTGGCTCACCCTCACGCCAGGCCTGC TCCTCACAACCAGCTCAGATGCTGTCAGTGGACACAGGCCATGCCGACAGGCAGGCCAGTGGCCGCATGG ACGTGTCAGCCTCTGTGGAGCAGGAGGCCCTGAGCAACGCCTTCCGCTCAGTGCCACTGGCTGAGGAGGA AGACTTTGACAGCAAGGAGTGGGTCATTATTGACAAGGAGACAGAGCTCAAGGACTTCCCTCCTGGGGCT GAGCCCAGCACATCAGGTACCACAGATGAAGAACCTGAGGAGCTGCGGCCTTTGCCTGAGGAGGGCGAGG AGAGGAGGCGTCTGGGGACAGAGCCTACTGTCAGGCCTCGTGGACGTGGTATGCAGACGCTGGCAGAGGA GGACCCACGGCAGATGCTGCCCCAGCCTGCACCACCTCAGTTGAGCCAGGCTGATGGCCGGTCAGAGACA TCGCAGCCTCCCACGCCTGGCAGCCCTTCCCACTCACCCCTGCACTCGGGACCCCGCCCTCGACGGAGAG AGTCGGATCCTACAGGCCCTCAGAGACAGGTGTTCTCTGTGGCGCCCCCGTTTGAGGTGAATGGCCTTCC ACGAGCTGTGCCTCTGGGCTTGCCCTACCAGGACTTCAAGAGAGACCTCTCTGATTATCGAGAACGGGCC CGGCTGCTCAACAGGGTCCGAAGGGTGGGCTTCTCGCACATGCTGCTCACCACACCGCAGGTCCCATTGG CTCCTTTCCAGCCTCAAGCCAATGGGAAAGAGGGTGAAGAGGAAGAGGAGGAGGATGAAGAGGAAGAGGA GGAAGAAGAGGAGGAAGATGAGGAAGACGAGGAGGAAGAGGATGAGGAAGATGAAGATGAGGAGGAGGAG GAGGCAGGGGCTCTTGGGGAGGTGCTGGGGCCTCGAAGCGGCTCCAGCAGTGAGGGGAGTGAACGGAGCC AGGAGGGCGCCCCATCCACACTGCTGGCTGACGACCAGAAAGAGGCCAGGGGCCGGGCCTCCATGGCTGA TGGGGACCTGGAGCCTGAGGAGGGCTCCAAAACGCTGGTGCTCGTCTCCCCTGGTGACATGAAGAAGTCA CCAGTCACTGCCGAACTGGCCCCTGACCCTGATCTGGGCACCTTGGCTGCACTCACCCCTCAGCACGAGC GGCCTCAGCCTACTGGAAGCCAGCTGGATGTGTCAGAGCCGGGCACCCTGTCCTCCATCCTCAAGTCTGA ACCCAAGCCCTCAGGACCTGGAGCAGGGGTTGGGGTTGGGCTGGTGGCCCCTGGGGCTGGGGTCACCGCA GTGACATCACCCTTCACCAAAGTCGAAAGGACCTTTGTTCACATTGCAGAGAAATCCCACCTCAATGTCA TGTCTTCCGGAGGACAGGCCCCCAGGCCAGAGGAGCTGAGCACTGGGGGCGAGTTGGGCCTAGAGATGCT CTCTGAGGTGGGCGCTGCAGAGGAGGGCGCCCCACAGCCTCTGGAGAACGGCATGGCTTTGGTAGGCCCG GATGGGACGGAGATGGAAAGTTGTGCCCTGTCTGGCCCTCCTGGGGAGACACCCTCAGAAGTAGTCACAG ACTCATTGCCCGATGGCCCAGCCCTTGCCGATGAACCGGCTCCAGCATCCCCACAAGAGCCAATAACCAA GAAAGGGACCATCATCTCCCCCAGCCGCCATGCCATGCCAGGATCTCGCCCCAGGAGCCGCATTCCTGTC TTGTTGTCTGAAGAGGACACAGGCTCAGAGCCTTCGGGCTCACTGTCGGCCAAAGAGCGGTGGAGCAAGA GGGCTCGGCCACAGCAGGATCTGGCTCGCCTCGTGATGGAGAAGAGGCAGGGTCGGCTACTGCTGCGGCT GGCCTCAGGGGCCTCCTCCTCCTCCAGCGAGGAGCAGCGCCGTGCCTCTGAGACACTCTCAGGCACCGGC TCCGAGGAGGACACGCCTGCCTCAGAGCCTGCAGCAGCCTTGCCTAGGAAGGCTGGGCGGGCAGCGACCA CCCGGAGCCGGATCCCCCGCCCCATCAGTGTGTCCATGCCTGCAGAAGGCCAGCAGCTTCCTGGTAGACC CCACGGTGCGGCCTCAGCGACCGACTCGGCCATCACCAGCAGGCTCCAGCTGCAGAAGCCCCCAGGGTTG GCCACTGCTGCTGACCACCGTCCCAAACAGCCCGCAAGCCGGGCTTCCGGCCCAGGTCGCGCCCAAGTCT CCAAGCCCGCAGCACCCCGCAGTCCCGGCCTCCCCACCTCCACCGCGCGCCACTCCAGCGGGTCCCCGCG GAGCCAGTCCCTGTCCCGCAAAGAGAGCTCCTCCCCCTCGCATCAAGCCCGGCCTGGTTTCCCTCCATCT CGGGGGGTCCTCCAAGTCAGATCTCAGTCGGAGGCCTCCCCGGGGGGCCCCAAGAAAGGACCCAAGGGGA AACAACTGCAGACTCTGCGCGCAGCAACCAAAGGCCGGCCGGCGGTTTCAGAGGGTCGGCCCGGGAACAG ATAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001271230
Insert Size	3924 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271230.1, NP_001258159.1
RefSeq Size	3924 bp
RefSeq ORF	3924 bp
Locus ID	316229

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