Nfasc (NM_001160314) Rat Tagged ORF Clone
CAT#: RR217559
- TrueORF®
Nfasc (myc-DDK-tagged) - Rat neurofascin (Nfasc), transcript variant 1
"NM_001160314" in other vectors (1)
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CNY 11,970.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | NF |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR217559 representing NM_001160314
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGGCAGCAGGCGCCACCCTGGGTCCACGTAGCCCTCATCCTCTTCCTCCTCAGCCTCGGAGGGG CCATTGAGATTCCGATGGATCCAAGCATTCAGAATGAGCTGACCCAACCCCCAACGATCACCAAGCAGTC GGTGAAGGACCACATCGTGGACCCCCGAGATAACATCCTGATTGAATGTGAAGCTAAAGGGAACCCCGCC CCCAGTTTCCACTGGACTCGCAACAGCAGGTTCTTCAACATTGCCAAGGACCCACGGGTGTCCATGAGGA GGAGGTCTGGGACCTTGGTGATCGACTTCCGCAGTGGTGGGCGGCCTGAGGAGTACGAAGGGGAGTACCA GTGCTTTGCCCGGAACAAATTCGGCACAGCTCTTAGCAACCGCATCCGCCTGCAGGTGTCCAAATCTCCC CTGTGGCCCAAGGAAAACCTAGACCCCGTCGTGGTTCAAGAGGGTGCCCCCTTAACCCTGCAGTGCAACC CCCCACCTGGCCTCCCATCCCCCGTCATCTTCTGGATGAGCAGCTCCATGGAGCCCATCACCCAGGACAA GCGTGTCTCCCAGGGTCACAACGGGGACCTGTACTTCTCCAACGTCATGCTGCAGGACATGCAGACCGAC TACAGCTGCAATGCACGCTTCCACTTCACCCACACCATTCAGCAGAAGAATCCCTTCACCCTCAAAGTCC TCACCACCCGAGGAGTTGCGGAAAGAACACCTAGCTTCATGTATCCCCAGGGCACGTCAAGCAGTCAGAT GGTACTGCGCGGCATGGACCTGCTGCTGGAGTGCATTGCCTCTGGGGTCCCAACACCAGATATTGCATGG TACAAGAAAGGTGGGGACCTCCCATCTGACAAGGCCAAGTTCGAGAATTTTAACAAGGCTCTGCGCATCA CCAATGTCTCTGAAGAGGACTCTGGGGAGTATTTCTGCCTGGCCTCCAACAAGATGGGCAGCATCCGGCA CACGATCTCGGTGAGAGTAAAGGCTGCCCCATACTGGCTGGATGAGCCCAAGAACTTGATCCTGGCTCCT GGTGAAGATGGGAGGCTGGTGTGTCGAGCCAATGGGAACCCGAAGCCGACCGTCCAGTGGCTGGTGAATG GAGACCCTTTGCAATCGGCACCACCCAACCCCAACCGTGAGGTGGCCGGAGACACTATCATCTTCCGGGA CACTCAGATCAGCAGCAGGGCAGTGTACCAGTGTAACACATCCAACGAACATGGCTACCTGCTGGCCAAT GCCTTCGTCAGCGTATTAGATGTACCCCCTCGGATGCTGTCTCCCCGGAACCAGCTCATCAGGGTGATCC TTTACAACCGGACGCGACTGGACTGTCCGTTCTTTGGGTCTCCCATTCCAACACTCCGATGGTTTAAGAA TGGGCAAGGAAGCAACCTGGATGGTGGTAACTACCACGTCTACGAAAATGGCAGCCTGGAAATCAAGATG ATTCGCAAAGAGGACCAAGGCATCTACACCTGTGTGGCCACCAACATCCTGGGAAAAGCTGAAAATCAAG TCCGCCTGGAGGTCAAAGACCCCACCAGGATCTACAGGATGCCTGAAGACCAGGTGGCCAAGAGGGGCAC CACAGTGCAGCTGGAGTGCCGTGTGAAGCATGACCCCTCCTTGAAACTCACAGTCTCCTGGCTGAAGGAT GACGAGCCACTCTACATTGGAAACAGGATGAAAAAGGAAGATGACTCCCTGACCATCTTCGGAGTGGCAG AGCGGGACCAAGGCAGTTACACGTGCATGGCCAGCACCGAGCTGGACCAGGACCTGGCAAAGGCCTACCT CACTGTTCTAGCTGATCAGGCCACTCCGACTAACCGTTTGGCTGCCCTGCCCAAAGGGCGGCCAGACCGA CCCAGGGACCTGGAGCTCACTGACCTGGCGGAAAGGAGTGTGAGGCTGACCTGGATCCCGGGGGATGATA ACAACAGTCCCATCACAGACTACGTTGTCCAGTTTGAAGAGGACCAGTTCCAGCCAGGAGTCTGGCATGA CCACTCCAAGTTCCCAGGCAGTGTCAACTCAGCCGTCCTCCATCTGTCCCCGTATGTCAACTATCAGTTC CGAGTCATCGCTGTCAACGAGGTTGGGAGCAGCCACCCCAGCCTTCCATCCGAGCGGTACCGAACAAGCG GGGCACCCCCTGAATCCAACCCCAGTGATGTGAAGGGCGAAGGGACACGAAAAAACAATATGGAGATCAC ATGGACGCCTATGAATGCTACCTCCGCCTTTGGCCCCAACCTGCGCTACATTGTCAAGTGGCGACGGAGA GAAACCCGAGAGACTTGGAACAATGTCACCGTGTGGGGCTCTCGCTATGTGGTGGGGCAGACCCCTGTCT ACGTACCCTATGAGATCCGAGTCCAGGCTGAAAATGACTTTGGGAAAGGCCCGGAGCCTGAAACCGTCAT TGGGTACTCGGGGGAAGATTTACCCAGTGCCCCCAGGCGTTTCAGAGTCCGACAGCCCAACCTGGAGACC ATCAACCTGGAATGGGATCACCCAGAGCACCCCAACGGGATCCTGATTGGATACACGCTCAGATACGTGC CCTTTAATGGAACCAAACTGGGAAAGCAGATGGTGGAAAACTTCTCTCCCAATCAGACCAAGTTCTCGGT GCAGAGAGCAGACCCCGTGTCCCGTTACCGCTTCTCCCTCAGTGCCAGGACGCAGGTGGGCTCTGGAGAA GCAGCCACGGAGGAGTCCCCAACACCTCCAAATGAAGCTACTCCAACTGCAGCTCCTCCCACATTGCCCC CGACTACCGTGGGTACCACAGGCCTTGTGAGCAGTACTGATGCTACTGCCCTTGCTGCCACCAGTGAAGC CACAACAGTTCCCATCATCCCAACGGTGGTACCTACCACCGTCGCCACCACCATTGCCACAACTACTACA ACCACTGCCGCCGCCACCACCACTACCACCACGGAGAGTCCTCCCACTACCACCACTGGGACTAAGATTC ACGAAACCGCCCCCGACGAGCAGTCCATTTGGAACGTCACGGTGCTCCCCAACAGTAAATGGGCCAACAT CACCTGGAAGCACAATTTCAGGCCTGGAACTGACTTTGTGGTTGAGTACATCGACAGCAACCATACGAAA AAAACTGTCCCTGTTAAGGCCCAAGCCCAGCCCATACAGCTGACAGACCTCTTTCCCGGGATGACGTACA CGTTGCGGGTGTATTCCCGGGACAACGAGGGCATCAGCAGTACCGTCATCACCTTTATGACCAGTACAGC TTACACCAACAACCAGACTGACATCGCCACCCAGGGCTGGTTCATCGGGCTCATGTGTGCCATTGCCCTT CTGGTGCTGATCCTGCTGATCGTCTGCTTCATCAAGAGGAGTCGTGGCGGCAAGTACCCAGTGCGAGAAA AGAAGGATGTCCCCTTGGGCCCTGAAGACCCCAAAGAAGAAGATGGTTCATTTGACTACAGTGACGAGGA CAACAAGCCCCTGCAGGGCAGCCAGACATCTCTGGACGGCACCATCAAGCAGCAGGAGAGTGACGACAGC CTAGTGGACTACGGCGAGGGTGGCGAGGGCCAGTTCAACGAAGATGGCTCCTTTATTGGCCAGTACACTG TCAGAAAGGACAAGGAGGAGACCGAGGGCAATGAGAGCTCAGAGGCCACATCTCCAGTCAATGCCATCTA TTCCCTGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR217559 representing NM_001160314
Red=Cloning site Green=Tags(s) MARQQAPPWVHVALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPA PSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSP LWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTD YSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAW YKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAP GEDGRLVCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLAN AFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKM IRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKD DEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDR PRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLHLSPYVNYQF RVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRR ETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPETVIGYSGEDLPSAPRRFRVRQPNLET INLEWDHPEHPNGILIGYTLRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGE AATEESPTPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTT TTAAATTTTTTESPPTTTTGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTK KTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQTDIATQGWFIGLMCAIAL LVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDS LVDYGEGGEGQFNEDGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001160314 |
ORF Size | 3720 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001160314.1, NP_001153786.1 |
RefSeq Size | 9825 bp |
RefSeq ORF | 3723 bp |
Locus ID | 116690 |
UniProt ID | P97685 |
MW | 138 kDa |
Gene Summary | This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined.[provided by RefSeq, May 2009] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN217559 | Nfasc (untagged) - Rat neurofascin (Nfasc), transcript variant 1 |
CNY 11,970.00 |