Inppl1 (NM_001270843) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Ship2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217530 representing NM_001270843 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCAGTGTGTGGGGCACCGAGTCCCGGGGGCGCGCTAGGCAGCCAGGCCCCTGCCTGGTATCACC GTGACCTGAGCCGCGCGGCTGCGGAGGAGCTGCTAGCTCGGGCAGGCCGCGATGGCAGCTTCCTGGTGCG AGACAGCGAGAGCGTGGCGGGGGCCTTCGCACTCTGCGTCCTGTATCAAAAGCACGTGCACACCTACCGC ATTCTGCCAGATGGAGAGGATTTCCTGGCTGTGCAGACCTCACAGGGCGTTCCTGTGCGCCGCTTCCAGA CCCTGGGTGAGCTTATAGGCCTGTATGCCCAGCCCAACCAGGGTCTTGTCTGTGCTCTGCTGCTGCCTGT AGAGGGGGAGAGAGAGCCAGATCCACCGGATGACCGAGATGCCTCAGATGTGGAGGACGAGAAACCCCCA CTACCCCCGCGCTCTGGCTCTACCAGCATTTCTGTCCCTGCGGGGCCTAGCAGCCCCCTGCCAGCCCCTG AGACTCCCACAACTCCAGCAGCTGAGAGCACTCCTAATGGACTCAGCACTGTGTCACATGAGTATCTGAA GGGCAGCTACGGGCTGGACCTGGAGGCTGTACGAGGCGGAGCCAGCAACTTGCCCCATCTCACCCGAACC CTTGTCACCTCATGCCGTAGGCTACACAGCGAGGTGGACAAGGTCCTGTCAGGCCTAGAGATCCTGTCGA AGGTGTTTGACCAGCAGAGCTCACCCATGGTGACCCGCCTTTTGCAGCAGCAGAGCCTACCACAGACTGG AGAGCAAGAGTTGGAGAGCCTTGTGCTGAAGCTATCTGTGCTAAAGGACTTCCTGTCAGGCATCCAGAAG AAGGCCCTAAAGGCACTGCAGGACATGAGCTCCACAGCACCTCCGGCTCCATTGCAGCCCTCCATACGAA AGGCCAAGACCATCCCTGTGCAAGCCTTTGAGGTGAAGCTGGATGTGACACTGGGTGACCTGACCAAGAT CGGGAAGTCCCAGAAGTTCACACTGAGCGTGGATGTGGAGGGTGGGAGGCTGGTACTGCTGAGGAGACAG CGTGACTCCCAGGAGGACTGGACGACCTTCACACACGACCGGATCCGGCAGCTCATTAAATCCCAGCGTG TGCAGAACAAGCTGGGTGTTGTGTTTGAAAAGGAGAAAGATCGGACGCAGCGCAAGGACTTCATCTTTGT CAGTGCCCGGAAGCGAGAAGCCTTCTGCCAGCTTCTGCAGCTCATGAAGAACAAGCATTCCAAGCAGGAT GAACCTGACATGATCTCCGTCTTCATAGGCACCTGGAACATGGGAAGTGTACCACCACCAAAAAACGTGA CATCTTGGTTCACATCAAAGGGACTGGGGAAAGCCCTGGATGAGGTCACAGTGACTATACCCCACGATAT CTATGTCTTTGGGACTCAGGAGAACTCAGTGGGTGACAGAGAGTGGCTGGATCTGCTGCGTGGGGGCCTC AAGGAGCTTACAGATCTGGATTACCGTCCGATTGCTATGCAGTCACTGTGGAACATCAAGGTGGCCGTGC TGGTCAAGCCAGAACATGAGAACCGCATCAGCCACGTTAGTACGTCCAGTGTGAAGACTGGTATCGCCAA TACCCTGGGGAACAAGGGAGCTGTGGGTGTTTCCTTCATGTTCAATGGCACTTCTTTTGGCTTCGTGAAT TGCCATCTCACCTCAGGGAATGAGAAGACTACTCGGCGGAACCAGAATTATCTGGACATCCTGCGTCTTC TCTCATTGGGTGATCGGCAGCTCAGTGCCTTTGACATCTCTTTGAGGTTCACTCATCTCTTCTGGTTTGG GGACCTTAACTACCGCTTAGACATGGATATCCAGGAGATCCTGAACTACATTAGTAGGAGAGAGTTTGAG CCCCTGCTCAGGGTGGACCAGCTCAACCTGGAGCGGGAGAAGCATAAGGTCTTCCTTCGATTTAGTGAGG AGGAGATATCTTTCCCACCCACCTACCGCTACGAGCGGGGTTCCCGAGACACATATGCTTGGCACAAGCA GAAGCCAACTGGGGTCCGGACCAATGTGCCTTCATGGTGTGACCGGATTCTATGGAAATCCTATCCTGAA ACCCACATCATCTGCAATTCCTATGGTTGCACTGATGACATTGTTACCAGTGACCATTCTCCTGTGTTTG GGACATTTGAGGTTGGAGTGACTTCCCAGTTCATCTCCAAGAAAGGTCTCTCTAAGACCTCAGACCAGGC CTACATTGAGTTTGAGAGCATCGAGGCCATCGTGAAGACGGCCAGCCGCACCAAGTTCTTCATTGAGTTC TATTCTACCTGCTTGGAAGAGTACAAGAAGAGCTTCGAGAATGACGCTCAGAGCAGTGACAACATCAATT TCCTCAAGGTGCAGTGGTCCTCGCGCCAGCTGCCCACGCTCAAGCCAATTCTGGCTGACATTGAGTACCT GCAGGATCAGCATCTCCTGCTCACAGTCAAGTCCATGGATGGCTACGAATCATATGGGGAGTGTGTGGTT GCACTCAAATCCATGATTGGCAGCACGGCCCAGCAGTTCTTGACCTTCTTGTCCCACCGTGGAGAGGAGA CAGGCAACATTCGTGGCTCCATGAAGGTGCGGGTGCCCACAGAACGCCTGGGCACCCGTGAGCGGCTCTA TGAATGGATTAGCATTGATAAGGATGACACAGGAGCCAAAAGCAAGGCTCCTTCAGTGTTGCGGGGCAGC CAGGAGCACAGATCTGGGAGCCGCAAGCCAACTTCCACAGAGGCCTCCTGTCCACTGTCCAAGTTGTTTG AAGAGCCTGAAAAGCCACCACCGACTGGCAGGCCCCCAGCCCCACCACGGGCAGTTCCTAGGGAGGAGTC CTTGAACCCCAGGTTGAAGTCAGAGGGGACACCTGAACAGGAAGGAGTAGCAGCCCCTCCACCCAAGAAC AGCTTCAATAACCCTGCCTACTACGTCCTTGAAGGGGTCCCACATCAGCTGCTGCCCCTGGAGCCAACCT CATTTGCCAGGGCCCCTATCCCACCTACCACCAAGAACAAAGTGGCCATCACAGTGCCTGCTCCTCAGCT TGGGCGCCACCGGACCCCTCGTGTGGGGGAGGGAAGCTCTTCGGATGAGGACTCTGGGGGCACACTGCCT CCTCCAGACTTCCCACCTCCACCACTGCCAGACTCAGCCATCTTCCTGCCCCCTAACCTGGATCCTTTAT CAATGCCAGTGGTCAGGGGCCGAAGTGTGGGTGAGGCCCGTGGCCCACCACCTCCCAAGGCCCATCCAAG ACCACCACTACCGCCGGGCACCTCACCTGCCAGTACTTTTTTGGAAGAGGTTGCAAGTGCGGATGACCGG TCTTGCTCAGTACTGCAGATGGCCAAGACACTCAGTGAGGTAGATTATTCTCCTGGGCCTGGACGCTCAG CACTCCTCCCCAACCCCTTGGAATTGCAGCTTCCCCGAGGGCCCTCGGACTACGGACGACCCCTCAGCTT CCCTCCACCCCGCATCCGGGAGAGCATCCAAGAAGACTTGGCAGAGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217530 representing NM_001270843 Red=Cloning site Green=Tags(s) MASVCGAPSPGGALGSQAPAWYHRDLSRAAAEELLARAGRDGSFLVRDSESVAGAFALCVLYQKHVHTYR ILPDGEDFLAVQTSQGVPVRRFQTLGELIGLYAQPNQGLVCALLLPVEGEREPDPPDDRDASDVEDEKPP LPPRSGSTSISVPAGPSSPLPAPETPTTPAAESTPNGLSTVSHEYLKGSYGLDLEAVRGGASNLPHLTRT LVTSCRRLHSEVDKVLSGLEILSKVFDQQSSPMVTRLLQQQSLPQTGEQELESLVLKLSVLKDFLSGIQK KALKALQDMSSTAPPAPLQPSIRKAKTIPVQAFEVKLDVTLGDLTKIGKSQKFTLSVDVEGGRLVLLRRQ RDSQEDWTTFTHDRIRQLIKSQRVQNKLGVVFEKEKDRTQRKDFIFVSARKREAFCQLLQLMKNKHSKQD EPDMISVFIGTWNMGSVPPPKNVTSWFTSKGLGKALDEVTVTIPHDIYVFGTQENSVGDREWLDLLRGGL KELTDLDYRPIAMQSLWNIKVAVLVKPEHENRISHVSTSSVKTGIANTLGNKGAVGVSFMFNGTSFGFVN CHLTSGNEKTTRRNQNYLDILRLLSLGDRQLSAFDISLRFTHLFWFGDLNYRLDMDIQEILNYISRREFE PLLRVDQLNLEREKHKVFLRFSEEEISFPPTYRYERGSRDTYAWHKQKPTGVRTNVPSWCDRILWKSYPE THIICNSYGCTDDIVTSDHSPVFGTFEVGVTSQFISKKGLSKTSDQAYIEFESIEAIVKTASRTKFFIEF YSTCLEEYKKSFENDAQSSDNINFLKVQWSSRQLPTLKPILADIEYLQDQHLLLTVKSMDGYESYGECVV ALKSMIGSTAQQFLTFLSHRGEETGNIRGSMKVRVPTERLGTRERLYEWISIDKDDTGAKSKAPSVLRGS QEHRSGSRKPTSTEASCPLSKLFEEPEKPPPTGRPPAPPRAVPREESLNPRLKSEGTPEQEGVAAPPPKN SFNNPAYYVLEGVPHQLLPLEPTSFARAPIPPTTKNKVAITVPAPQLGRHRTPRVGEGSSSDEDSGGTLP PPDFPPPPLPDSAIFLPPNLDPLSMPVVRGRSVGEARGPPPPKAHPRPPLPPGTSPASTFLEEVASADDR SCSVLQMAKTLSEVDYSPGPGRSALLPNPLELQLPRGPSDYGRPLSFPPPRIRESIQEDLAEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001270843
ORF Size	3549 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270843.1, NP_001257772.1
RefSeq Size	4640 bp
RefSeq ORF	3552 bp
Locus ID	65038
UniProt ID	Q9WVR3
MW	131.1 kDa
Gene Summary	phosphatase that plays a negative regulatory role in insulin-induced mitogenesis [RGD, Feb 2006]

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