Inppl1 (NM_001270843) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Ship2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217530 representing NM_001270843 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCAGTGTGTGGGGCACCGAGTCCCGGGGGCGCGCTAGGCAGCCAGGCCCCTGCCTGGTATCACC GTGACCTGAGCCGCGCGGCTGCGGAGGAGCTGCTAGCTCGGGCAGGCCGCGATGGCAGCTTCCTGGTGCG AGACAGCGAGAGCGTGGCGGGGGCCTTCGCACTCTGCGTCCTGTATCAAAAGCACGTGCACACCTACCGC ATTCTGCCAGATGGAGAGGATTTCCTGGCTGTGCAGACCTCACAGGGCGTTCCTGTGCGCCGCTTCCAGA CCCTGGGTGAGCTTATAGGCCTGTATGCCCAGCCCAACCAGGGTCTTGTCTGTGCTCTGCTGCTGCCTGT AGAGGGGGAGAGAGAGCCAGATCCACCGGATGACCGAGATGCCTCAGATGTGGAGGACGAGAAACCCCCA CTACCCCCGCGCTCTGGCTCTACCAGCATTTCTGTCCCTGCGGGGCCTAGCAGCCCCCTGCCAGCCCCTG AGACTCCCACAACTCCAGCAGCTGAGAGCACTCCTAATGGACTCAGCACTGTGTCACATGAGTATCTGAA GGGCAGCTACGGGCTGGACCTGGAGGCTGTACGAGGCGGAGCCAGCAACTTGCCCCATCTCACCCGAACC CTTGTCACCTCATGCCGTAGGCTACACAGCGAGGTGGACAAGGTCCTGTCAGGCCTAGAGATCCTGTCGA AGGTGTTTGACCAGCAGAGCTCACCCATGGTGACCCGCCTTTTGCAGCAGCAGAGCCTACCACAGACTGG AGAGCAAGAGTTGGAGAGCCTTGTGCTGAAGCTATCTGTGCTAAAGGACTTCCTGTCAGGCATCCAGAAG AAGGCCCTAAAGGCACTGCAGGACATGAGCTCCACAGCACCTCCGGCTCCATTGCAGCCCTCCATACGAA AGGCCAAGACCATCCCTGTGCAAGCCTTTGAGGTGAAGCTGGATGTGACACTGGGTGACCTGACCAAGAT CGGGAAGTCCCAGAAGTTCACACTGAGCGTGGATGTGGAGGGTGGGAGGCTGGTACTGCTGAGGAGACAG CGTGACTCCCAGGAGGACTGGACGACCTTCACACACGACCGGATCCGGCAGCTCATTAAATCCCAGCGTG TGCAGAACAAGCTGGGTGTTGTGTTTGAAAAGGAGAAAGATCGGACGCAGCGCAAGGACTTCATCTTTGT CAGTGCCCGGAAGCGAGAAGCCTTCTGCCAGCTTCTGCAGCTCATGAAGAACAAGCATTCCAAGCAGGAT GAACCTGACATGATCTCCGTCTTCATAGGCACCTGGAACATGGGAAGTGTACCACCACCAAAAAACGTGA CATCTTGGTTCACATCAAAGGGACTGGGGAAAGCCCTGGATGAGGTCACAGTGACTATACCCCACGATAT CTATGTCTTTGGGACTCAGGAGAACTCAGTGGGTGACAGAGAGTGGCTGGATCTGCTGCGTGGGGGCCTC AAGGAGCTTACAGATCTGGATTACCGTCCGATTGCTATGCAGTCACTGTGGAACATCAAGGTGGCCGTGC TGGTCAAGCCAGAACATGAGAACCGCATCAGCCACGTTAGTACGTCCAGTGTGAAGACTGGTATCGCCAA TACCCTGGGGAACAAGGGAGCTGTGGGTGTTTCCTTCATGTTCAATGGCACTTCTTTTGGCTTCGTGAAT TGCCATCTCACCTCAGGGAATGAGAAGACTACTCGGCGGAACCAGAATTATCTGGACATCCTGCGTCTTC TCTCATTGGGTGATCGGCAGCTCAGTGCCTTTGACATCTCTTTGAGGTTCACTCATCTCTTCTGGTTTGG GGACCTTAACTACCGCTTAGACATGGATATCCAGGAGATCCTGAACTACATTAGTAGGAGAGAGTTTGAG CCCCTGCTCAGGGTGGACCAGCTCAACCTGGAGCGGGAGAAGCATAAGGTCTTCCTTCGATTTAGTGAGG AGGAGATATCTTTCCCACCCACCTACCGCTACGAGCGGGGTTCCCGAGACACATATGCTTGGCACAAGCA GAAGCCAACTGGGGTCCGGACCAATGTGCCTTCATGGTGTGACCGGATTCTATGGAAATCCTATCCTGAA ACCCACATCATCTGCAATTCCTATGGTTGCACTGATGACATTGTTACCAGTGACCATTCTCCTGTGTTTG GGACATTTGAGGTTGGAGTGACTTCCCAGTTCATCTCCAAGAAAGGTCTCTCTAAGACCTCAGACCAGGC CTACATTGAGTTTGAGAGCATCGAGGCCATCGTGAAGACGGCCAGCCGCACCAAGTTCTTCATTGAGTTC TATTCTACCTGCTTGGAAGAGTACAAGAAGAGCTTCGAGAATGACGCTCAGAGCAGTGACAACATCAATT TCCTCAAGGTGCAGTGGTCCTCGCGCCAGCTGCCCACGCTCAAGCCAATTCTGGCTGACATTGAGTACCT GCAGGATCAGCATCTCCTGCTCACAGTCAAGTCCATGGATGGCTACGAATCATATGGGGAGTGTGTGGTT GCACTCAAATCCATGATTGGCAGCACGGCCCAGCAGTTCTTGACCTTCTTGTCCCACCGTGGAGAGGAGA CAGGCAACATTCGTGGCTCCATGAAGGTGCGGGTGCCCACAGAACGCCTGGGCACCCGTGAGCGGCTCTA TGAATGGATTAGCATTGATAAGGATGACACAGGAGCCAAAAGCAAGGCTCCTTCAGTGTTGCGGGGCAGC CAGGAGCACAGATCTGGGAGCCGCAAGCCAACTTCCACAGAGGCCTCCTGTCCACTGTCCAAGTTGTTTG AAGAGCCTGAAAAGCCACCACCGACTGGCAGGCCCCCAGCCCCACCACGGGCAGTTCCTAGGGAGGAGTC CTTGAACCCCAGGTTGAAGTCAGAGGGGACACCTGAACAGGAAGGAGTAGCAGCCCCTCCACCCAAGAAC AGCTTCAATAACCCTGCCTACTACGTCCTTGAAGGGGTCCCACATCAGCTGCTGCCCCTGGAGCCAACCT CATTTGCCAGGGCCCCTATCCCACCTACCACCAAGAACAAAGTGGCCATCACAGTGCCTGCTCCTCAGCT TGGGCGCCACCGGACCCCTCGTGTGGGGGAGGGAAGCTCTTCGGATGAGGACTCTGGGGGCACACTGCCT CCTCCAGACTTCCCACCTCCACCACTGCCAGACTCAGCCATCTTCCTGCCCCCTAACCTGGATCCTTTAT CAATGCCAGTGGTCAGGGGCCGAAGTGTGGGTGAGGCCCGTGGCCCACCACCTCCCAAGGCCCATCCAAG ACCACCACTACCGCCGGGCACCTCACCTGCCAGTACTTTTTTGGAAGAGGTTGCAAGTGCGGATGACCGG TCTTGCTCAGTACTGCAGATGGCCAAGACACTCAGTGAGGTAGATTATTCTCCTGGGCCTGGACGCTCAG CACTCCTCCCCAACCCCTTGGAATTGCAGCTTCCCCGAGGGCCCTCGGACTACGGACGACCCCTCAGCTT CCCTCCACCCCGCATCCGGGAGAGCATCCAAGAAGACTTGGCAGAGGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270843
Insert Size	3552 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270843.1, NP_001257772.1
RefSeq Size	4640 bp
RefSeq ORF	3552 bp
Locus ID	65038
UniProt ID	Q9WVR3
Gene Summary	phosphatase that plays a negative regulatory role in insulin-induced mitogenesis [RGD, Feb 2006] Transcript Variant: This variant (2) differs in the 5' UTR and lacks an internal exon in the 3' coding region, compared to variant 1. The resulting isoform (2) is C-terminal truncated, compared to isoform 1.

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